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The aim is to investigate the application of periodontal tissue regeneration combined with orthodontics in oral restoration, and explore its effect and significance on the expressions of Interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin-5 (IL-5) in periodontal tissue. The patients in observation group were treated with orthodontics combined with periodontal tissue regeneration, and the control group was treated with periodontal tissue regeneration. The total effective rate, adverse reactions, recurrence rate and treatment satisfaction were compared. The masticatory function, language function, aesthetic level, VAS score, quality of life, gingival index (GI), plaque index (PLI), periodontal pocket probing depth (PD), sulcus bleeding index (SBI), IL-1β, TNF-α and IL-5 levels were compared. The recurrence rate of observation group was lower than control group, while the treatment satisfaction was higher after treatment. After treatment, the scores of masticatory, language, aesthetics, physiological, social, emotional, cognitive, and emotional functions and overall health score were higher than before treatment. After treatment, the scores of masticatory and language functions, aesthetics and quality of life of observation group were significantly higher than control group. After treatment, the VAS score, GI, PLI, SBI, PD, IL-1β, TNF-α and IL-5 levels were lower than before. The VAS score, GI, PLI, SBI, PD levels, IL-1β, TNF-α and IL-5 levels of observation group were lower after treatment. Orthodontics combined with periodontal tissue regeneration can help improve the periodontal condition of patients with periodontitis, reduce inflammatory response, improve the level of efficacy and overall safety, and further improve patients' quality of life and treatment satisfaction. [ABSTRACT FROM AUTHOR]

Objective The present study investigated the effects of the inflammatory microenvironment mediated by macrophages on the proliferation and osteogenic differentiation of periodontal ligament cells (PDLCs). Methods Conditioned medium containing inflammatory factors was collected following macrophage activation with 1 μg/mL lipo⁃ polysaccharide (LPS). PDLCs were isolated from healthy teeth and cultured in conditioned medium (LPS⁃CM group) or normal medium (control group), and the proliferation of PDLCs was detected using the MTT assay. The cells were cocul⁃ tured with an osteogenic inducer for 3 d and 7 d, and the alkaline phosphatase (ALP) activity of PDLCs was detected us⁃ing an ALP kit. The mRNA expression levels of runt⁃related transcription factor 2 (RUNX2), osteocalcin (OCN), and col⁃ lagen I (COL⁃I) were detected using real⁃time PCR, and the protein levels of RUNX2, OCN, and COL⁃I were detected using Western blotting. Mineralization nodules were observed using Alizarin red staining after osteoinduction for 14 d. Results The OD value of PDLCs in the LPS⁃CM group was lower than that in the control group (P < 0.05). The mRNA levels of RUNX2, OCN, and COL⁃I in the LPS⁃CM group were lower than those in the control group (P < 0.05). In addi⁃ tion to the OCN 3 d group (t = 2.75, P = 0.056), the protein expression of RUNX2, OCN, and COL⁃I in the LPS⁃CM group was lower than that in the control group (P < 0.05). However, the ALP activity of the LPS⁃CM group was higher than that of the control group, which was 1.58⁃fold greater (t = 5.91, P = 0.030) at 3 d and 1.29⁃fold greater (t = 6.01, P = 0.046) at 7 d. The number of calcified nodules in the LPS⁃CM group was significantly less than that in the control group (t = 8.63, P = 0.048). Conclusion The inflammatory microenvironment mediated by macrophages may inhibit the proliferation and osteogenic differentiation of PDLCs.

This study investigated the mechanisms through which leader emotional intelligence (EI) influences employee work performance by exploring the mediating effect of basic psychological needs and the moderating effect of employee EI. We surveyed 239 pairs of leaders and their direct subordinates in various Chinese enterprises and processed the data using structural equation modeling and path analysis. The results showed that leader EI was significantly and positively correlated with employee work performance, and there was also a significant interaction effect of leader EI and employee EI on work performance. Regardless of employees' EI levels, higher EI in leaders was associated with higher levels of employee work performance through improving employees' basic psychological needs satisfaction. These findings provide guidance for enhancing personal leadership skills in practical work settings and ultimately achieving the goal of improving both employee work performance and organizational effectiveness. [ABSTRACT FROM AUTHOR]

Neuroimaging studies have indicated that altered cerebral blood flow (CBF) was associated with the long-term symptoms of postacute sequelae of SARS-CoV-2 infection (PASC), also known as “long COVID”. COVID-19 and long COVID were found to be strongly associated with host gene expression. Nevertheless, the relationships between altered CBF, clinical symptoms, and gene expression in the central nervous system (CNS) remain unclear in individuals with long COVID. This study aimed to explore the genetic mechanisms of CBF abnormalities in individuals with long COVID by transcriptomic-neuroimaging spatial association. Lower CBF in the left frontal-temporal gyrus was associated with higher fatigue and worse cognition in individuals with long COVID. This CBF pattern was spatially associated with the expression of 2,178 genes, which were enriched in the molecular functions and biological pathways of COVID-19. Our study suggested that lower CBF is associated with persistent clinical symptoms in long COVID individuals, possibly as a consequence of the complex interactions among multiple COVID-19-related genes, which contributes to our understanding of the impact of adverse CNS outcomes and the trajectory of development to long COVID. [ABSTRACT FROM AUTHOR]

Background: Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease and has become a public health concern worldwide. The hallmark of NAFLD is hepatic steatosis. Therefore, there is an urgent need to develop new therapeutic strategies that are efficacious and have minimal side effects in hepatic steatosis and NAFLD treatment. The present study aimed to investigate the effect of dietary supplement of curcumin on high-fat diet (HFD)-induced hepatic steatosis and the underlying mechanism. Methods: ApoE−/− mice were fed a normal diet, high-fat diet (HFD) or HFD supplemented with curcumin (0.1% w/w) for 16 weeks. Body and liver weight, blood biochemical. parameters, and liver lipids were measured. Intestinal permeability, hepatic steatosis and mRNA and protein expressions of TLR4-related inflammatory signaling molecule were analyzed. Results: The administration of curcumin significantly prevented HFD-induced body weight gain and reduced liver weight. Curcumin attenuated hepatic steatosis along with improved serum lipid profile. Moreover, curcumin up-regulated the expression of intestinal tight junction protein zonula occluden-1 and occludin, which further improved gut barrier dysfunction and reduced circulating lipopolysaccharide levels. Curcumin also markedly down-regulated the protein expression of hepatic TLR4 and myeloid differentiation factor 88 (MyD88), inhibited p65 nuclear translocation and DNA binding activity of nuclear factor-κB (NF-κB) in the liver. In addition, the mRNA expression of hepatic tumour necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) as well as the plasma levels of TNF-α and IL-1β were also lowered by curcumin treatment. Conclusion: These results indicated that curcumin protects against HFD-induced hepatic steatosis by improving intestinal barrier function and reducing endotoxin and liver TLR4/NF-κB inflammation. The ability of curcumin to inhibit hepatic steatosis portrayed its potential as effective dietry intervention for NAFLD prevention. [ABSTRACT FROM AUTHOR]