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Strategies have not been available until recently to uncover interacting protein networks specific to key cell types, their subcellular compartments, and their major regulators during complex in vivo events. Here we apply BioID2 proximity labeling to capture protein networks acting within cardiomyocytes during a key model of innate heart regeneration in zebrafish. Transgenic zebrafish expressing a promiscuous BirA2 localized to the entire myocardial cell or membrane compartment were generated, each identifying distinct proteomes in adult cardiomyocytes that became altered during regeneration. BioID2 profiling for interactors with ErbB2, a co-receptor for the cardiomyocyte mitogen Nrg1, implicated Rho A as a target of ErbB2 signaling in cardiomyocytes. Blockade of Rho A during heart regeneration, or during cardiogenic stimulation by the mitogenic influences Nrg1, Vegfaa or Vitamin D, disrupted muscle creation. Our findings reveal proximity labeling as a useful resource to interrogate cell proteomes and signaling networks during tissue regeneration in zebrafish., SCOPUS: ar.j, info:eu-repo/semantics/published

A study of the stripping voltammetry of hemispherical deposits under electrochemically irreversible conditions is presented. Experiments show a difference in the stripping voltammetry of bismuth from a single crystal Au(111) electrode where the bismuth covers the surface in relatively flat film and a boron-doped diamond (BDD) electrode where the hemispherical deposits are seen on the surface. It is shown using mathematical modeling and numerical simulation that this difference cannot be accounted for by simply considering the different distributions of bismuth on the electrode surfaces. Rather, it is concluded that the difference in voltammetry is mainly due to the morphology/orientation of the deposits formed leading to differences in the kinetics and thermodynamics of the stripping process.