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486 results on '"Zheng, Beiwen"'

6. Small wards in the ICU: a favorable measure for controlling the transmission of carbapenem-resistant Klebsiella pneumoniae

Author

Chi, Xiaohui, Meng, Xiaohua, Xiong, Luying, Chen, Tao, Zhou, Yanzi, Ji, Jinru, and Zheng, Beiwen

Subjects
Medical research -- Analysis -- Health aspects, Medicine, Experimental -- Analysis -- Health aspects, Bacterial pneumonia -- Health aspects -- Analysis, Genomics -- Analysis -- Health aspects, Drug resistance in microorganisms -- Analysis -- Health aspects, Pneumonia -- Health aspects -- Analysis, Medical colleges -- Health aspects -- Analysis, Phylogeny -- Health aspects -- Analysis, Disease transmission -- Analysis -- Health aspects, Hospital patients -- Analysis -- Health aspects, Health care industry
Abstract

Purpose Carbapenem-resistant Klebsiella pneumoniae (CRKP) is one of the leading causes of healthcare-associated infections (HAIs) and is particularly pervasive in intensive care units (ICUs). This study takes ICU layout as the research object, and integrates clinical data and bacterial genome analysis to clarify the role of separate, small wards within the ICU in controlling the transmission of CRKP. Methods This study prospectively observed the carriage and spread of CRKP from a long-term in-hospital patient (hereafter called the Patient) colonized with CRKP in the gut and located in a separate, small ward within the ICU. The study also retrospectively investigated CRKP-HAIs in the same ICU. The relationship and transmission between CRKP isolates from the Patient and HAI events in the ICU were explored with comparative genomics. Results In this study, 65 CRKP-HAI cases occurred during the investigation period. Seven CRKP-HAI outbreaks were also observed. A total of 95 nonrepetitive CRKP isolates were collected, including 32 strains from the Patient in the separate small ward. Phylogenetic analysis based on core genome single-nucleotide polymorphism (cgSNP) showed that there were five possible CRKP clonal transmission events and two clonal outbreaks (A1, A2) during the study. CRKP strains from the Patient did not cause CRKP between-patient transmission or outbreaks in the ICU during the 5-year study period. Conclusion The presence of a long-term hospitalized patient carrying CRKP and positioned in a separate, small ward did not lead to CRKP transmission or infection outbreaks in the ICU. Combining a small-ward ICU layout with normative HAI control measures for multidrug-resistant pathogen infection was effective in reducing CRKP transmission., Author(s): Xiaohui Chi [sup.1], Xiaohua Meng [sup.1], Luying Xiong [sup.1], Tao Chen [sup.1], Yanzi Zhou [sup.1], Jinru Ji [sup.1], Beiwen Zheng [sup.1] [sup.2] [sup.3], Yonghong Xiao [sup.1] [sup.2] [sup.3] Author [...]

Published
2022
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14. An integrated nationwide genomics study reveals transmission modes of typhoid fever in China

Author

Feng, Ye, primary, Pan, Hang, additional, Zheng, Beiwen, additional, Li, Fang, additional, Teng, Lin, additional, Jiang, Zhijie, additional, Feng, Mengyao, additional, Zhou, Xiao, additional, Peng, Xianqi, additional, Xu, Xuebin, additional, Wang, Haoqiu, additional, Wu, Beibei, additional, Xiao, Yonghong, additional, Baker, Stephen, additional, Zhao, Guoping, additional, and Yue, Min, additional

Published
2023
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28. Complete-genome sequencing and comparative genomic characterization of blaNDM-5 carrying Citrobacter freundii isolates from a patient with multiple infections.

Author

Ye, Jianzhong, Jin, Lulu, Li, Yaling, Xu, Hao, Lin, Yishuai, Zhou, Tieli, Zheng, Beiwen, Wang, Maofeng, and Wang, Zhongyong

Subjects
CITROBACTER freundii, COMPARATIVE genomics, URINARY tract infections, MICROBIAL sensitivity tests, PLASMID genetics, HORIZONTAL gene transfer, ESCHERICHIA coli
Abstract

Background: The emergence and wide spread of carbapenemase-producing Enterobacteriaceae (CPE) poses a growing threat to global public health. However, clinically derived carbapenemase-producing Citrobacter causing multiple infections has rarely been investigated. Here we first report the isolation and comparative genomics of two blaNDM-5 carrying Citrobacter freundii (C. freundii) isolates from a patient with bloodstream and urinary tract infections. Results: Antimicrobial susceptibility testing showed that both blaNDM-5 carrying C. freundii isolates were multidrug-resistant. Positive modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) results suggested metallo-carbapenemase production. PCR and sequencing confirmed that both metallo-carbapenemase producers were blaNDM-5 positive. Genotyping and comparative genomics analyses revealed that both isolates exhibited a high level of genetic similarity. Plasmid analysis confirmed that the blaNDM-5 resistance gene is located on IncX3 plasmid with a length of 46,161 bp, and could successfully be transferred to the recipient Escherichia coli EC600 strain. A conserved structure sequence (ISAba125-IS5-blaNDM-5-trpF-IS26-umuD-ISKox3) was found in the upstream and downstream of the blaNDM-5 gene. Conclusions: The data presented in this study showed that the conjugative blaNDM-5 plasmid possesses a certain ability to horizontal transfer. The dissemination of NDM-5-producing C. freundii isolates should be of close concern in future clinical surveillance. To our knowledge, this is the first study to characterize C. freundii strains carrying the blaNDM-5 gene from one single patient with multiple infections. [ABSTRACT FROM AUTHOR]

Published
2023
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30. Intestinal colonization with ESBL-producing Klebsiella pneumoniae in healthy rural villager: A genomic surveillance study in China, 2015-2017

Author

Wang, Shuang, primary, Xie, Hengjie, additional, Chen, Yuzhen, additional, Liu, Lu, additional, Fang, Ming, additional, Sun, Dapeng, additional, Xu, Liuchen, additional, Bi, Zhenqiang, additional, Sun, Gaoxiang, additional, Li, Yan, additional, Yu, Xiaolin, additional, Zhang, Huaning, additional, Kou, Zengqiang, additional, and Zheng, Beiwen, additional

Published
2022
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31. The impact of dietary fibers on Clostridioides difficile infection in a mouse model

Author

Wu, Zhengjie, primary, Xu, Qiaomai, additional, Wang, Qiangqiang, additional, Chen, Yunbo, additional, Lv, Longxian, additional, Zheng, Beiwen, additional, Yan, Ren, additional, Jiang, Huiyong, additional, Shen, Jian, additional, Wang, Shuting, additional, Wang, Kaicen, additional, Xia, Jiafeng, additional, Han, Shengyi, additional, and Li, Lanjuan, additional

Published
2022
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39. Case Report: Metagenomic Next-Generation Sequencing Clinches the Diagnosis of Acute Q Fever and Verified by Indirect Immunofluorescence Assay

Author

Yang, Yide, Shi, Qingmiao, Jin, Qian, Yang, Zhangnv, Li, Wangfang, Han, Jianfeng, Mao, Juanjuan, and Zheng, Beiwen

Subjects
General Medicine
Abstract

Q fever is a zoonotic infectious disease caused by Coxiella burnetii. The clinical symptoms of acute Q fever are usually atypical, and routine serological tests of C. burnetii are not readily available, making the diagnosis of Q fever a challenge. In this case, we report a male patient who had repeated fevers and was administered empirical anti-infective treatment, but the effect was poor. After conducting relevant laboratory and imagological examinations, the etiology has not yet been confirmed. Subsequently, metagenomic next-generation sequencing (mNGS) identified the sequence reads of C. burnetii from the patient's peripheral blood within 48 h, and then the diagnosis of acute Q fever was established. Moreover, the serological test of indirect immunofluorescence assay (IFA) of the C. burnetii antibody was further performed in the Centers for Disease Control, certifying the result of mNGS. The patient was ultimately treated with doxycycline and recovered well. mNGS is an unbiased and comprehensive method in infrequent or culture-negative pathogen identification. To our knowledge, this is the first case of acute Q fever identified by mNGS and confirmed by IFA in Taizhou, China. A further large-scale prospective clinical cohort study is worth carrying out to compare the diagnostic efficiency of mNGS with traditional serological methods and PCR in acute Q fever.

Published
2022

40. Akkermansia muciniphila Ameliorates Clostridioides difficile Infection in Mice by Modulating the Intestinal Microbiome and Metabolites

Author

Wu, Zhengjie, Xu, Qiaomai, Gu, Silan, Chen, Yunbo, Lv, Longxian, Zheng, Beiwen, Wang, Qiangqiang, Wang, Kaicen, Wang, Shuting, Xia, Jiafeng, Yang, Liya, Bian, Xiaoyuan, Jiang, Xianwan, Zheng, Lisi, and Li, Lanjuan

Subjects
Microbiology (medical), Microbiology
Abstract

Clostridioides difficile is a common cause of nosocomial infection. Antibiotic-induced dysbiosis in the intestinal microbiota is a core cause of C. difficile infection (CDI). Akkermansia muciniphila plays an active role in maintaining gastrointestinal balance and might offer the protective effects on CDI as probiotics. Here, we investigated the effects and mechanisms of A. muciniphila on CDI. C57BL/6 mice (n = 29) were administered A. muciniphila MucT (3 × 109 CFUs, 0.2 mL) or phosphate-buffered saline (PBS) by oral gavage for 2 weeks. Mice were pretreated with an antibiotic cocktail and subsequently challenged with the C. difficile strain VPI 10463. A. muciniphila treatment prevented weight loss in mice and reduced the histological injury of the colon. And it also alleviated inflammation and improved the barrier function of the intestine. The administration effects of A. muciniphila may be associated with an increase in short-chain fatty acid production and the maintenance of bile acids’ steady-state. Our results provide evidence that administration of A. muciniphila to CDI mice, with an imbalance in the microbial community structure, lead to a decrease in abundance of members of the Enterobacteriaceae and Enterococcaceae. In short, A. muciniphila shows a potential anti-CDI role by modulating gut microbiota and the metabolome.

Published
2022

46. Emergence of mcr-8.2-harboring hypervirulent ST412 Klebsiella pneumoniae strain from pediatric sepsis: a comparative genomic survey

Author

Liu, Ruishan, Xu, Hao, Zhao, Junhui, Hu, Xinjun, Wu, Lingjiao, Qiao, Jie, Ge, Haoyu, Guo, Xiaobing, Gou, Jianjun, and Zheng, Beiwen

Abstract

Emerging mobile colistin resistance (mcr) genes pose a significant threat to public health for colistin was used as the last resort to treat multidrug-resistant (MDR) pathogenic bacterial infections. Hypervirulent Klebsiella pneumoniae (hvKP) is a clinically significant pathogen resulting in highly invasive infections, often complicated by devastating dissemination. Worryingly, the untreatable and severe infections caused by mcr-harboring hvKP lead to the selection of antibiotics for clinical anti-infective treatment in a dilemma. Herein, we screened 3,461 isolates from a tertiary teaching hospital from November 2018 to March 2021, and an mcr-8.2-harboring hvKP FAHZZU2591 with a conjugative plasmid was identified from pediatric sepsis. This is the first report of MCR-8-producing hvKP from pediatric sepsis to our best knowledge. The susceptibility, genetic features, and plasmid profiles of the isolate were investigated. Further, we assessed the virulence potential of FAHZZU2591 and verified its pathogenicity and invasive capacity using a mouse model. The phylogenetic analysis of mcr-8-bearing K. pneumoniae revealed that China is the predominant reservoir of the mcr-8 gene, and the clinic is the primary source. Our work highlights the risk for the spread of mcr-positive hvKP in clinical, especially in pediatric sepsis, and the persistent surveillance of colistin-resistance hvKP is urgent.

Published
2022
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50. Comparative Respiratory Tract Microbiome Between Carbapenem-Resistant Acinetobacter baumannii Colonization and Ventilator Associated Pneumonia

Author

Xiao, Tingting, primary, Guo, Qian, additional, Zhou, Yanzi, additional, Shen, Ping, additional, Wang, Yuan, additional, Fang, Qiang, additional, Li, Mo, additional, Zhang, Shuntian, additional, Guo, Lihua, additional, Yu, Xiao, additional, Liao, Yulin, additional, Wang, Chunhui, additional, Chi, Xiaohui, additional, Kong, Xiaoyang, additional, Zhou, Kai, additional, Zheng, Beiwen, additional, Luo, Qixia, additional, Chen, Yunbo, additional, Zhu, Huaiqiu, additional, and Xiao, Yonghong, additional

Published
2022
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