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The purpose of this study was to investigate and analyze the difficulties and the influencing factors in the laparoscopic surgery for appendiceal abscess in children.Forty-six patients with appendiceal abscess receiving laparoscopic surgery (laparoscopic surgery group) in Xuzhou Children's Hospital from January 2012 to March 2017 were retrospectively analyzed. The surgery was performed using the 3-hole method, the 30° lens was placed in umbilical region, and the left lower abdomen and superior pubis were the operating holes. If the appendix was thick and big, 10 mm trocar was replaced on the superior pubis and the appendix was removed, followed by pelvic or lower abdominal drainage via the superior pubis incision.The laparoscopic surgery group was compared with 45 cases of laparotomy (traditional surgery group) for appendiceal abscess performed by the same group of operators in the past, and the perioperative period and postoperative complications between the two groups were compared. The child patients' age, history of abdominal pain and prediagnosis treatment were compared within the laparoscopic surgery group. Compared with those in traditional surgery group, the surgery time in laparoscopic surgery group had no statistically significant difference (Pgt;0.05), the incidence rate of complications was lower, and the difference was statistically significant (Plt;0.05). In laparoscopic surgery group, the surgery time of child patients aged ≥6 years old with the medical history for more than 5 days and unreasonable preoperative treatment was longer than that of child patients agedlt;6 years old with the medical history for less than or equal to 5 days and reasonable preoperative treatment, and the difference was statistically significant (Plt;0.05).Compared with the traditional surgery, laparoscopic surgery for appendiceal abscess in children can significantly reduce the postoperative complications; at the same time, the history of abdominal pain and preoperative treatment are the influencing factors of surgery.

Background: To investigate the expressions of fibrinogen (Fib) and Interleukin-12 (IL-12) in serum of neonatal necrotizing enterocolitis (NEC), and to analyze the correlation between the two and their relationship with clinicopathological features. Methods: Forty two children with NEC treated in Xuzhou Children’s Hospital, Xuzhou Medical University Xuzhou, China from 2016-2019 were selected as an observation group and 40 children who underwent physical examination at the same period as a control group. The expression levels of Fib and IL-12 in the serum of two groups were detected by ELISA. The correlation between Fib and IL-12 in the observation group and the correlation among the expressions of Fib, IL-12, the clinicopathological features and common examination indexes of the children with NEC were investigated by Pearson correlation analysis. Results: The levels of Fib and IL-12 in the serum of the children in observation group were significantly higher than those in the control group were (P

Objective To investigate the relationship between FOXO3 overexpression and NLRP3 and explore the effect of FOXO3 on necrotizing colitis. Methods 100 clean grade newborn SD (Sprague Dawley) rats were randomly divided into 4 groups: NEC group, NEC + FOXO3a group, NEC + NC group and control group. NEC rat model was established by hypoxia + hypothermia stimulation; HE staining was used for detection of the inflammation of intestinal tissue. The histological scores of intestinal tissues were histologically scored, generally, there were three types of inflammatory scoring systems including anatomically based systems, severity-based systems and quality of life systems (Lim et al., 2015) and in this study we utilized severity-based systems by HE staining. Human intestinal epithelial cell line was transfected with recombinant plasmid overexpressing FOXO3a and recombinant plasmid overexpressing NLRP3, and divided into control group, LPS group, LPS + NC group, LPS + FOXO3a group and LPS + FOXO3a + NLRP3 group; Caspase-1 was used for the detection of pyroptosis. The expressions of FOXO3a, NLRP3, cleaved Caspase-1 and the expression of TLR4 in TLR4 signaling pathway were detected by RT-qPCR and WB. IL-1β, IL-6, IL-18 and TNF-α were detected by ELISA. Results (1) FOXO3a is under-expressed and NLRP3 is highly expressed in NEC neonatal rat intestinal tissue. (2) The inflammatory condition of intestinal tissue in NEC + FOXO3a group was improved compared with NEC group (P (3) FOXO3a was highly expressed in NEC + FOXO3a group. The expression of IL-1β, IL-6, IL-18, SOD and MDA in NEC + FOXO3a group was lower than that in NEC group. (4) The expression of IL-1β, IL-6, IL-18, SOD and MDA in intestinal epithelial cells of LPS + FOXO3a group was lower than other groups. (5) Overexpression of FOXO3a inhibits LPS-induced pyroptotic cell death in intestinal epithelial cells by inhibiting NLRP3. Conclusion Overexpression of FOXO3 in mice with necrotizing colitis can improve inflammatory conditions in mice by affecting NLRP3-mediated cell caking.

Archaeological wood is a kind of ‘new material’ that has deteriorated due to long-term degradation. The existing wood science theory and evaluation methods are not fully applicable to archaeological wood. Moreover, current physical-mechanical evaluation methods are inadequate for fragile archaeological wood due to their insufficient accuracy and the large sample amount required, causing difficulties in many necessary physical-mechanical repeatability tests. In light of these limitations, the representative samples on Nanhai No. 1, a merchant shipwreck in the Song Dynasty, were selected as the research objects in this paper. The shipwreck is a typical waterlogged wooden artifact. A quasi-nondestructive physical-mechanical evaluation technique for archaeological wood was developed with the thermomechanical analyzer (TMA). This study used TMA to evaluate the bending strength of representative waterlogged archaeological samples of Nanhai No. 1 shipwreck and sound wood with the same species. Besides, the thermal linear expansion coefficients in the ambient temperature range were obtained. The sizes of the samples used in the tests were only 2 mm × 8 mm × 0.3 mm and 1 cm × 1 cm × 1 cm, respectively. Bending strength results of archaeological wood by the TMA method conformed to the tendency that the bending strength decreases with the increase of decay degree. In addition, the longitudinal linear expansion coefficients of archaeological wood reached 80%–115% of those in the transverse grain direction, which were about 10 times higher than those of the sound wood. The linear expansion coefficients of archaeological wood in three directions were similar. Based on the results of Fourier transform infrared analysis (FT-IR), the significant differences in the physical-mechanical properties of the archaeological wood and the sound wood were induced to be mainly ascribed to the decomposition and the loss of hemicellulose in the archaeological wood. The cell wall substrate could not stabilize the cellulose skeleton, which led to the instability of the tracheid structure of the archaeological wood. This study provided a proven quasi-nondestructive method for the preservation state evaluation of waterlogged archaeological wood (WAW) from the Nanhai I shipwreck and other similar waterlogged wooden relics.

The present study determined the changes in the expression levels of MYPT1, CPI-17 and MLC20 in the ileum of mice with neonatal induced necrotizing enterocolitis (NEC) to provide a basis for a pathogenesis model that includes smooth muscle changes during NEC. A group of 7-day-old BALB/c mice were fed with formula (40 µl/g, 5 times/day) and given hypoxia treatments (5% O2 and 95% N2 for 10 min, twice daily) for 4 days to induce NEC and establish a mouse model. A control group of 7-day-old BALB/c mice were left with their mother for the duration of the treatment. After establishing the model, the two groups of mice were sacrificed, and the terminal ileum tissue was collected and subjected to western blot analysis and immunohistochemistry. The results showed the expression levels of MYPT1 and pMYPT1 in the ileum of the mice in the NEC group were lower than those in the control group (P

Backgrounds Necrotizing enterocolitis (NEC) was one of the main causes of morbidity and mortality in neonates. Our objective was to detect the mechanism of miR-124 in small bowel tissues of NEC. Methods Hematoxylin and eosin (H&E) staining was used to detect the repair of the damaged tissues in rat NEC model. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was used to evaluate the cell apoptosis level in intestinal tissue. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the messenger RNA (mRNA) expression level of miR-124, Rho-associated coiled-coil-containing protein kinase 1 (ROCK1), myosin phosphatase target subunit 1 (MYPT1), and Toll-like receptor 9 (TLR9) in NEC tissues and IEC-6 cells. Luciferase reporter assay was used to verify whether ROCK1 is a direct target of miR-124. Results miR-124 was overexpressed in the NEC tissues, while ROCK1 and MYPT1 was downregulated in the NEC tissues. Inhibition of miR-124, suppressed the intestinal cell apoptosis and promoted the expression of ROCK1 and MYPT1. What is more, overexpression of miR-124 could inhibit the expression of ROCK1, TLR9, and MYPT1. Luciferase assay confirmed that miR-124 can regulate the transcriptional activity of ROCK1 through binding its 3'-UTR region. Conclusion miR-124 was a promoter of NEC, which promotes the intestine cell apoptosis and inflammatory cell infiltration through the inhibition of TLR9 expression by targeting ROCK1.

A variety of novel 3-trifluoromethyl substituted pyrazole oxime ether derivatives containing a pyridyl moiety were designed and synthesized in satisfactory yields. Their structures were confirmed by H NMR, C NMR, and elemental analysis. The preliminary bioassays indicated that some of the title compounds showed potential insecticidal activities against Aphis craccivora, and some compounds also displayed plant growth regulatory activities.

Eleven new thiazol-containing 1H-1,2,4-triazole derivatives were designed and synthesized by utilizing 3,3-dimethylbutan-2-one as starting material. Their structures were characterized by IR, 1 H NMR, 13 C NMR, and elemental analysis. Furthermore, some compounds’ structures were confirmed with MS and X-ray diffraction and the biological activities of these compounds were evaluated, including in vitro fungicidal and plant-growth regulatory activities. The primary bioassay results indicated that these compounds showed low antifungal activities while exhibited promising plant-growth regulatory activities.

The N-substituted alkyl sulfoximine derivatives are a new chemical family of neonicotinoid insecticides. We have designed and synthesized 10 (1,3-thiazole)alkyl sulfoximine derivatives. All compounds were identified by (1)H and (13)C nuclear magnetic resonance (NMR), IR, and elemental analyses. Preliminary bioassays indicated that some title compounds exhibited good insecticidal activities at 10 mg/L against Myzus persicae . The relationship between structure and biological activity was also discussed.

Two series of novel 2-cyano-3-benzylaminoacrylates containing long-chain thiazole ring moiety were synthesized as herbicidal inhibitors of photosystem II (PS II) electron transportation. Their structures were confirmed by 1H NMR and elemental analysis. The bioassay showed that these compounds retain high herbicidal activities and especially compounds 13a and 13h have excellent herbicidal activities.

In order to find potent pesticide containing heterocycle, a series of novel pyrazole oxime ether derivatives contain- ing a thiazolyl moiety were designed and synthesized by introducing the significant thiazole ring into the molecular structure of pyrazole. The structures of these compounds were characterized by 1 H NMR, 13 C NMR spectra and elemental analysis. Bioactivities of the target compounds were performed, and some compounds exhibited certain fungicidal, insecticidal or plant