41 results on '"Zhao MN"'
Search Results
2. 51‐1: Invited Paper:Foveated Image Compression and Transmission for Virtual‐Reality Headsets
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Jia, T, Shen, C, Shi, ZH, Chen, FB, Zhang, QF, Zhao, MN, Yu, YL, Zhang, R, and Wang, CH
- Abstract
Here we propose a foveated image transmission and compensation strategy with a rendering compression step, a re‐compression step and a decompression step. It is realized and tested on an FPGA‐based headset tester equipped with a high‐accuracy eye tracking system. Past human factors engineering experiments are reviewed and used to determine the proper boundaries for thefoveation zone division.
- Published
- 2024
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3. Changes and significance of the fibrinolytic system following two pulmonary thromboembolisms in a rabbit model.
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Liu DC, Duan B, Zhao MN, Wu L, Cao YZ, Liu NB, Xue Z, He ZH, and Mi J
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- Animals, Rabbits, Male, RNA, Messenger metabolism, RNA, Messenger genetics, Lung metabolism, Pulmonary Embolism metabolism, Pulmonary Embolism blood, Pulmonary Embolism pathology, Plasminogen Activator Inhibitor 1 metabolism, Plasminogen Activator Inhibitor 1 genetics, Tissue Plasminogen Activator metabolism, Tissue Plasminogen Activator genetics, Disease Models, Animal, Fibrinolysis
- Abstract
In this study, we examined the changes in the fibrinolytic system in a rabbit model of two acute pulmonary thromboembolisms (PTE). Fourteen healthy adult New Zealand white rabbits were divided into three groups: the single PTE group (five rabbits), the double PTE group (five rabbits), and the control group (four rabbits). A rabbit model of acute pulmonary embolism was established, and immunohistochemistry and polymerase chain reaction (PCR) were performed on tissue plasminogen activator (t-PA), plasminogen activator inhibitor-1 (PAI-1) in plasma, and pulmonary embolism tissue. Plasma results: 1) t-PA levels: one hour following the initial modeling, the levels of t-PA in the modeling groups were significantly lower than those in the control group (P<0.05). In addition, the t-PA levels in the double PTE group were found to be lower after the modeling, as compared to the pre-modeling period (P<0.05). One hour after the second modeling, the double PTE group had lower t-PA levels compared to the control group (P<0.05). However, t-PA rebounded two hours after modeling in the double PTE group. One week after the second modeling, the double PTE group had higher t-PA levels compared to the other two groups (P<0.05). 2) PAI-1 results: one hour after the initial modeling, PAI-1 levels in the two modeling groups were lower compared to the pre-modeling period and control groups (P<0.05). Two hours following modeling, PAI-1 levels in both modeling groups were lower compared to the control group (P<0.05). PAI-1 levels were lower in the double PTE group one and two hours after the second modeling compared to the other two groups and pre-modeling period (P<0.05). 3) The immunohistochemistry results: the expression of PAI-1 decreased in the two modeling groups, while t-PA expression increased compared to the control group. 4) PCR results: t-PA mRNA expression did not differ among the three groups. The PAI-1 mRNA expression was lower in the two PTE groups compared to the control group. We conclude that in the early stages of PTE, the local fibrinolytic activity of the thrombus is increased, which is favorable for thrombolysis. However, as the thrombus persists, the activity of the fibrinolytic system is inhibited, contributing to the development of chronic thromboembolic pulmonary hypertension.
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- 2024
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4. Identification of the HLA-A*11:463 allele in a Chinese patient and his father.
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Zhao MN, Shi XM, and Gao SJ
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- Humans, Male, Base Sequence, Alleles, China, Fathers, Sequence Analysis, DNA, Nucleotides, HLA-A Antigens genetics
- Abstract
HLA-A*11:463 has one nucleotide change from HLA-A*11:01:01:01 at nucleotide 508 changing Lysine (146) to Glutamine., (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
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- 2024
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5. DMF as an amine source: iron-catalyzed cyclization of 2 H -azirines to imidazoles.
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Zhao MN, Yang ZM, and Li LQ
- Abstract
A novel method has been developed for the synthesis of 1-methyl-4,5-diaryl-1 H -imidazoles through Fe(II)-catalyzed cyclization of 2 H -azirines and N , N -dimethylformamide (DMF) as an amine source. This transformation involves the cleavage of C-N and CN double bonds and the construction of new C-N and CN double bonds. The reaction has readily available starting materials, a wide range of substrates and mild reaction conditions. In addition, the reaction also facilitated the convenient synthesis of 1-methyl-2,4,5-triaryl-1 H -imidazoles.
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- 2024
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6. [Mechanism of Kuntai Capsules in treatment of polycystic ovary syndrome rat models based on AGE-RAGE signal pathway].
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Huang JG, Zhang J, Xiang RJ, Wu YY, He TY, Zhao MN, and Wang F
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- Humans, Rats, Female, Animals, bcl-2-Associated X Protein, Saline Solution, Rats, Sprague-Dawley, Follicular Atresia, Signal Transduction, Body Weight, Follicle Stimulating Hormone, RNA, Messenger, Polycystic Ovary Syndrome drug therapy, Polycystic Ovary Syndrome genetics
- Abstract
This study aims to investigate the impact of Kuntai Capsules(KTC) on polycystic ovarian syndrome(PCOS) rat models and explore the underlying mechanism. Fifty female SD rats were randomly divided into five groups(10 rats in each group), including control group, model group, low-, medium-, and high-dose KTC group. Except for the control group, the other groups were injected with dehydroepiandrosterone(DHEA) combined with a high-fat diet(HFD) to induce the PCOS rat model for 28 days. 0.315, 0.63, and 1.26 g·kg~(-1)·d~(-1) KTC was dissolved in the same amount of normal saline and given to low-, medium-, and high-dose KTC groups by gavage. Both control group and model group were given the same amount of normal saline for 15 days. After administration, fasting blood glucose(FBG) was measured by a glucose meter. Fasting insulin(FINS), luteinizing hormone(LH), testosterone(T), and follicle-stimulating hormone(FSH) were detected by enzyme-linked immunosorbent assay(ELISA), and LH/FSH ratio and insulin resistance index(HOMA-IR) were calculated. The pathological morphology of ovarian tissue was observed by hematoxylin-eosin(HE) staining. The expression levels of collagen α type Ⅲ 1 chain(COL3A1), apoptotic factors Bax, and Bcl-2 were detected using Western blot and immunofluorescence. The mRNA expressions of COL3A1, Bax, and Bcl-2 in ovarian tissue were performed by real-time PCR(RT-PCR). The results show that compared with the control group, the body weight, serum levels of FBG, FINS, LH, T, LH/FSH, and HOMA-IR are higher in model group(P<0.05 or P<0.01), and the level of FSH is lower(P<0.05). In model group, a large number of white blood cells are found in the vaginal exfoliated cells, mainly in the interictal phase. There are more cystic prominences on the surface of the ovary. The thickness of the granular cell layer is reduced, and oocytes are absent. COL3A1 and Bax protein expression levels are increased(P<0.01), while Bcl-2 protein expression levels are decreased(P<0.05) in the ovarian tissue COL3A1 and Bax mRNA expression levels are increased in ovarian tissue(P<0.05). Compared with the model group, the body weight, FBG, FINS, LH, T, LH/FSH, and HOMA-IR in low-, medium-, and high-dose KTC groups are decreased(P<0.05 or P<0.01), while the levels of FSH in medium-, and high-dose KTC groups are increased(P<0.05 or P<0.01). Low-, medium-, and high-dose KTC groups gradually show a stable interictal phase. The surface of the ovary is smooth. Oocytes and mature follicles can be seen in ovarian tissue, and the thickness of the granular cell layer is increased. The expression level of COL3A1 protein decreases in low-and medium-dose KTC groups(P<0.05 or P<0.01), and that of Bax protein decreases in low-dose KTC group(P<0.05 or P<0.01), and the expression level of Bcl-2 protein increases in low-dose KTC group(P<0.01). The expression levels of COL3A1 and Bax mRNA decreased in the low-dose KTC group(P<0.05), while the expression levels of Bcl-2 mRNA increased(P<0.05). In summary, KTC can inhibit ovarian granulosa cell apoptosis and reduce follicular atresia by regulating the AGE-RAGE signaling pathway. It can promote insulin secretion, reduce blood sugar and body weight, restore serum hormone levels, improve symptoms of PCOS, alleviate morphological damage of the ovary, and restore ovarian function, which is of great value in the treatment of PCOS.
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- 2024
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7. Shikonin Exerts an Antileukemia Effect against FLT3-ITD Mutated Acute Myeloid Leukemia Cells via Targeting FLT3 and Its Downstream Pathways.
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Zhao MN, Su L, Song F, Wei ZF, Qin TX, Zhang YW, Li W, and Gao SJ
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- Humans, Animals, Mice, Signal Transduction drug effects, Cell Line, Tumor, Tandem Repeat Sequences, Xenograft Model Antitumor Assays, Antineoplastic Agents pharmacology, Antineoplastic Agents therapeutic use, fms-Like Tyrosine Kinase 3 genetics, fms-Like Tyrosine Kinase 3 antagonists & inhibitors, fms-Like Tyrosine Kinase 3 metabolism, Naphthoquinones pharmacology, Naphthoquinones therapeutic use, Leukemia, Myeloid, Acute drug therapy, Leukemia, Myeloid, Acute genetics, Leukemia, Myeloid, Acute pathology, Leukemia, Myeloid, Acute metabolism, Mutation, Apoptosis drug effects, Cell Proliferation drug effects
- Abstract
Introduction: Acute myeloid leukemia (AML) with internal tandem duplication (ITD) mutations in Fms-like tyrosine kinase 3 (FLT3) has an unfavorable prognosis. Recently, using newly emerging inhibitors of FLT3 has led to improved outcomes of patients with FLT3-ITD mutations. However, drug resistance and relapse continue to be significant challenges in the treatment of patients with FLT3-ITD mutations. This study aimed to evaluate the antileukemic effects of shikonin (SHK) and its mechanisms of action against AML cells with FLT3-ITD mutations in vitro and in vivo., Methods: The CCK-8 assay was used to analyze cell viability, and flow cytometry was used to detect cell apoptosis and differentiation. Western blotting and real-time polymerase chain reaction were used to examine the expression of certain proteins and genes. Leukemia mouse model was created to evaluate the antileukemia effect of SHK against FLT3-ITD mutated leukemia in vivo., Results: After screening a series of leukemia cell lines, those with FLT3-ITD mutations were found to be more sensitive to SHK in terms of proliferation inhibition and apoptosis induction than those without FLT3-ITD mutation. SHK suppresses the expression and phosphorylation of FLT3 receptors and their downstream molecules. Inhibition of the NF-κB/miR-155 pathway is an important mechanism through which SHK kills FLT3-AML cells. Moreover, a low concentration of SHK promotes the differentiation of AML cells with FLT3-ITD mutations. Finally, SHK could significantly inhibit the growth of MV4-11 cells in leukemia bearing mice., Conclusion: The findings of this study indicate that SHK may be a promising drug for the treatment of FLT3-ITD mutated AML., (© 2023 S. Karger AG, Basel.)
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- 2024
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8. Atorvastatin ameliorated myocardial fibrosis in db/db mice by inhibiting oxidative stress and modulating macrophage polarization.
- Author
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Song XM, Zhao MN, Li GZ, Li N, Wang T, and Zhou H
- Abstract
Background: People with diabetes mellitus (DM) suffer from multiple chronic complications due to sustained hyperglycemia, especially diabetic cardiomyopathy (DCM). Oxidative stress and inflammatory cells play crucial roles in the occurrence and progression of myocardial remodeling. Macrophages polarize to two distinct phenotypes: M1 and M2, and such plasticity in phenotypes provide macrophages various biological functions., Aim: To investigate the effect of atorvastatin on cardiac function of DCM in db/db mice and its underlying mechanisms., Methods: DCM mouse models were established and randomly divided into DM, atorvastatin, and metformin groups. C57BL/6 mice were used as the control. Cardiac function was evaluated by echocardiography. Hematoxylin and eosin and Masson staining was used to examine the morphology and collagen fibers in myocardial tissues. The expression of transforming growth factor-β1 (TGF-β1), tumor necrosis factor-α (TNF-α), interleukin-1 β (IL-1β),M1 macrophages (iNOS
+ ), and M2 macrophages (CD206+ ) were demonstrated by immunohistochemistry and immunofluorescence staining. The levels of TGF-β1, IL-1β, and TNF-α were detected by ELISA and real-time quantitative polymerase chain reaction. Malondialdehyde (MDA) concentrations and superoxide dismutase (SOD) ac-tivities were also measured., Results: Treatment with atorvastatin alleviated cardiac dysfunction and decreased db/db mice. The broken myocardial fibers and deposition of collagen in the myocardial interstitium were relieved especially by atorvastatin treatment. Atorvastatin also reduced the levels of serum lactate dehydrogenase, creatine kinase isoenzyme, and troponin; lowered the levels of TGF-β1, TNF-α and IL-1β in serum and myocardium; decreased the concentration of MDA and increased SOD activity in myocardium of db/db mice; inhibited M1 macrophages; and promoted M2 macrophages., Conclusion: Administration of atorvastatin attenuates myocardial fibrosis in db/db mice, which may be associated with the antioxidative stress and anti-inflammatory effects of atorvastatin on diabetic myocardium through modulating macrophage polarization., Competing Interests: Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article., (©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.)- Published
- 2023
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9. CNIH4 governs cervical cancer progression through reducing ferroptosis.
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Yang JY, Ke D, Li Y, Shi J, Wan SM, Wang AJ, Zhao MN, and Gao H
- Abstract
Cervical cancer is one of the most leading causes of cancer death worldwide, and ferroptosis is implicated in the progression of cervical cancer. Cornichon family AMPA receptor auxiliary protein 4 (CNIH4) is involved in the progression of various human cancers; however, its function in cervical cancer remains unclear. The present study aims to investigate the role and mechanism of CNIH4 in cervical cancer using gain- and loss-of-function studies in vitro. SiHa and CaSki cells were infected with lentiviral vectors to manipulate the expression of CNIH4 in vitro, and cell viability, migration, invasion as well as ferroptosis were evaluated. Transcriptome sequencing analysis was performed to further validate the mechanism through which CNIH4 regulated the progression of cervical cancer. The expression of CNIH4 was upregulated in human cervical cancer tissues and cells, and strongly correlated with the decreases in overall survival and disease free survival rates of cervical cancer patients. CNIH4 silence inhibited, while CNIH4 overexpression facilitated the survival of human cervical cancer cells. Mechanistically, CNIH4 elevated solute carrier family 7 member 11 (SLC7A11)-mediated cystine import, and subsequently increased intracellular glutathione synthesis and glutathione peroxidase 4 activity, thereby inhibiting ferroptosis of human cervical cancer cells. SLC7A11 silence significantly abolished CNIH4-mediated inhibition of ferroptosis in cervical cancer cells in vitro. Our study for the first time reveals that CNIH4 inhibits ferroptosis of human cervical cancer cells through upregulating SLC7A11, defining CNIH4 as an attractive therapeutic and prognostic target for cervical cancer., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)
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- 2023
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10. A novel microRNA-182/Interleukin-8 regulatory axis controls osteolytic bone metastasis of lung cancer.
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Zhao MN, Zhang LF, Sun Z, Qiao LH, Yang T, Ren YZ, Zhang XZ, Wu L, Qian WL, Guo QM, Xu WX, Wang XQ, Wu F, Wang L, Gu Y, Liu MF, and Lou JT
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- Animals, Humans, Mice, Cell Line, Tumor, Cell Proliferation, Gene Expression Regulation, Neoplastic, Interleukin-8 metabolism, Mice, Nude, Neoplasm Metastasis, Carcinoma, Non-Small-Cell Lung pathology, Lung Neoplasms pathology, MicroRNAs metabolism
- Abstract
Bone metastasis is one of the main complications of lung cancer and most important factors that lead to poor life quality and low survival rate in lung cancer patients. However, the regulatory mechanisms underlying lung cancer bone metastasis are still poor understood. Here, we report that microRNA-182 (miR-182) plays a critical role in regulating osteoclastic metastasis of lung cancer cells. We found that miR-182 was significantly upregulated in both bone-metastatic human non-small cell lung cancer (NSCLC) cell line and tumor specimens. We further demonstrated that miR-182 markedly enhanced the ability of NSCLC cells for osteolytic bone metastasis in nude mice. Mechanistically, miR-182 promotes NSCLC cells to secrete Interleukin-8 (IL-8) and in turn facilitates osteoclastogenesis via activating STAT3 signaling in osteoclast progenitor cells. Importantly, systemically delivered IL-8 neutralizing antibody inhibits NSCLC bone metastasis in nude mice. Collectively, our findings identify the miR-182/IL-8/STAT3 axis as a key regulatory pathway in controlling lung cancer cell-induced osteolytic bone metastasis and suggest a promising therapeutic strategy that targets this regulatory axis to interrupt lung cancer bone metastasis., (© 2023. The Author(s).)
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- 2023
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11. First-line treatments for advanced hepatocellular carcinoma: a network meta-analysis and cost-effectiveness analysis in China and the United States.
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Sun KX, Cao SS, Shi FH, Guan Y, Tang M, Zhao MN, Jian YF, Cui B, Li ZY, Wang JW, Yu F, and Ding Y
- Abstract
Background: Various therapeutic strategies are available for the first-line treatment of patients with advanced hepatocellular carcinoma (aHCC). But which approach is the most cost-effective remains uncertain., Objectives: This study aims to evaluate the cost-effectiveness of first-line strategies in aHCC patients from the perspective of Chinese and US payers., Design: A network meta-analysis (NMA) and cost-effectiveness study., Data Sources and Methods: A NMA was conducted to collect all first-line strategies with aHCC from 1 October 1 2018 until 1 January 2022. The relevant randomized controlled trial literature in PubMed, Embase, and Cochrane Library for the last 3 years were searched. The abstracts of meetings of the American Society of Clinical Oncology, European Society of Medical Oncology, and American Association for Cancer Research were also reviewed. A Markov model that included three states was developed. One-way sensitivity and probabilistic sensitivity analysis were performed to investigate the uncertainty of the economic evaluation. Scenario analysis was conducted to explore the economic benefits of treatment strategies in low-income populations., Results: Base-case analysis in China included 1712 patients showed that atezolizumab combined with bevacizumab, sintilimab combined with bevacizumab, lenvatinib (LEVA), and sorafenib (SORA) added 0.46, 1.25, 0.77, and -1.08 quality-adjusted life-years (QALYs), respectively, compared with donafenib, resulting in an incremental cost-effective ratio of $85607.88, $12109.27, and $1651.47 per QALY at a willingness-to-pay (WTP) of $11101.70/QALY. In the United States, only the incremental cost-effectiveness ratios (ICERs) of SORA was higher that were lower than the WTP threshold ($69375/QALY), and LEVA was the most cost-effective strategy with the ICERs were 25022.13/QALY., Conclusion: The NMA and cost-effectiveness analysis revealed that LEVA is the favorite choice in the first-line treatment of Chinese aHCC patients and US payers' perspective when the WTP was $11101.70/QALY in China and $69375.0/QALY in the United States., Registration: This study has been registered on the PROSPERO database with the registration number CRD42021286575., Competing Interests: The authors declare that there is no conflict of interest., (© The Author(s), 2022.)
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- 2022
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12. Iodine-Mediated Cyclization of Enamines to Imidazole-4-Carboxylic Derivatives with Sequential Removal of Nitrogen Atoms from TMSN 3 .
- Author
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Gao P, Chen H, Bai ZJ, Zhang S, Zhao MN, Yang D, Li Y, Zhang J, and Wang X
- Abstract
An iodine-mediated oxidative [4+1] cyclization of enamines with TMSN
3 for the synthesis of 2,5-disubstituted imidazole-4-carboxylic derivatives has been developed. The mechanistic studies revealed that the reaction proceeds through a sequential removal of two nitrogen atoms from TMSN3 . The synthetic utility was further demonstrated with a gram-scale reaction and various derivatization transformations of the products.- Published
- 2021
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13. Iron-Catalyzed Cycloaddition of Amides and 2,3-Diaryl-2 H -azirines To Access Oxazoles via C-N Bond Cleavage.
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Zhao MN, Ning GW, Yang DS, Fan MJ, Zhang S, Gao P, and Zhao LF
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A novel and efficient iron-catalyzed cycloaddition reaction using readily available 2,3-diaryl-2 H -azirines and primary amides is reported. A wide range of trisubstituted oxazoles could be achieved in good yields with good functional group compatibility. In this transformation, two C-N bonds were cleaed and new C-N and C-O bonds were formed.
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- 2021
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14. [Improvement effect of proanthocyanidins on cell viability of rotenone-induced Parkinson's disease model].
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Xu YL, Xu Y, Li HF, Shen SH, Ruan WL, Guo XH, Zhao MN, Han YY, and Zhang H
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- Apoptosis drug effects, Autophagy drug effects, Cell Line, Tumor, Cell Survival drug effects, Humans, Reactive Oxygen Species, Rotenone toxicity, Neuroprotective Agents pharmacology, Parkinson Disease drug therapy, Proanthocyanidins pharmacology
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- 2020
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15. IBX-Promoted Oxidative Cyclization of N -Hydroxyalkyl Enamines: A Metal-Free Approach toward 2,3-Disubstituted Pyrroles and Pyridines.
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Gao P, Chen HJ, Bai ZJ, Zhao MN, Yang D, Wang J, Wang N, Du L, and Guan ZH
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An iodoxybenzoic acid-mediated selected oxidative cyclization of N -hydroxyalkyl enamines was developed. Through this strategy, a variety of 2,3-disubstituted pyrroles and pyridines were produced in good selectivity involving oxidation of alcohol, followed by condensation of aldehyde and α-C of enamines. Furthermore, this metal-free method has several advantages, including the use of environmentally friendly reagents, broad substrate scope, mild reaction conditions, and high efficiency.
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- 2020
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16. Corrigendum to "Application of vacuum-ultraviolet (VUV) for phenolic homologues removal in humic acid solution: Efficiency, pathway and DFT calculation" [J. Hazard. Mater. 384 (2020) 121464].
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Cao TT, Xu TF, Zhao MN, Jiang X, and Cui CW
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- 2020
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17. Application of vacuum-ultraviolet (VUV) for phenolic homologues removal in humic acid solution: Efficiency, pathway and DFT calculation.
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Cao TT, Tie-Fu X, Zhao MN, Xu J, and Cui CW
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Vacuum-ultraviolet (VUV) photo-initiated oxidation of phenolic homologues in simulative natural water were investigated, including phenol, o-dihydroxybenzene (ODB), m-dihydroxybenzene (MDB), p-dihydroxybenzene (PDB), paranitrophenol (PNP) and o-chlorophenol (OCP). Results showed the phenolic homologues removal rate reached at least 90% in pure water, which was dependent on temperature, pH, concentration of HA, and functional group of HA. Experimental results indicated that 0.2 mg/L HA might be a critical point. Additionally, the rate constant of the six phenolic homologues reduced by 76.85%, 77.81%, 71.91%, 79.15%, and 55.69%, respectively in the MDB solution, and 79.73%, 82.80%, 95.36%, 80.38%, and 92.64%, respectively in the benzoic acid (BA) solution, compared to the rate constant in pure water. Moreover, quantum chemistry calculation indicated that the variances between phenolic compounds in removal rate were attributed to the substituent on the benzene ring. And, to some extent, the carboxy group of HA was supposed to arose the suppression for phenolic homologues removal rate. Mechanism involved phenolic homologues degradation using vacuum-ultraviolet (VUV) was summarized, where it underwent the formation of quinone structures, ring opening, short-chain organic acid, even eventually the transformation into NO
3 - and Cl- of PNP and OCP., (Copyright © 2019. Published by Elsevier B.V.)- Published
- 2020
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18. [Effects of CeO 2 nanoparticles on the viabilities of neural PC12 and SH-SY5Y cells].
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Xu YL, Zhao MN, Zhu SH, Han YY, Zhang JY, Shen SH, Yu ZS, and Zhang H
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- Animals, Cell Line, Tumor, Humans, PC12 Cells, Rats, Reactive Oxygen Species metabolism, Cell Survival, Cerium pharmacology, Nanoparticles, Neurons drug effects
- Abstract
Objective: To investigate the effects of cerium oxide (CeO
2 ) nanoparticles on the viabilities of nerve cells PC12 and SH-SY5Y., Methods: CeO2 nanoparticles were synthesized, structures were characterized and properties were evaluated. PC12 cells and SH-SY5Y cells were treated with CeO2 nanoparticles at different concentrations (1, 2.5, 5, 10, 25, 50, 75, 100, 150 μg/ml) for 24 h and the cell viability was measured by MTT assay. Then PC12 cells and SH-SY5Y cells were co-treated with CeO2 and active oxygen scavenger NAC and the cells were stained with DCFH-DA probe for ROS. The number of cells and the fluorescence intensity were observed under a fluorescent inverted microscope. Differences were assessed by one-way ANOVA., Results: After treatment with CeO2 nanoparticles, the viabilities of both PC12 cells (P<0.01) and SH-SY5Y cells (P<0.01) were decreased comparing with the control group. After staining with DCFH-DA probe, the fluorescence intensity of the nerve cells was enhanced depending on the concentration of CeO2 nanoparticles suggesting that CeO2 induced the generation of reactive oxygen species (ROS). The fluorescence intensity of PC12 cells was decreased after CeO2 nanoparticles (100 μg/ml) co-treatment with active oxygen scavenger NAC. Compared with CeO2 nanoparticles alone at 25 μg/ml (P<0.01), 50 μg/ml (P<0.01), 75 μg/ml (P<0.01), 100 μg/ml (P<0.01), the cell viability was significantly increased after co-treatment with NAC., Conclusion: CeO2 nanoparticles has a negative effect on the viabilities of nerve cells PC12 and SH-SY5Y, and the effect might be depend on ROS.- Published
- 2020
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19. Application of single balloon enteroscopy-assisted therapeutic endoscopic retrograde cholangiopancreatography in patients after bilioenteric Roux-en-Y anastomosis: Experience of multi-disciplinary collaboration.
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Wu WG, Qin LC, Song XL, Zhao MN, Zhang WJ, Gu J, Weng H, Liu YB, Zhang Y, Qu CY, Xu LM, and Wang XF
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- Aged, Aged, 80 and over, China epidemiology, Cholangiopancreatography, Endoscopic Retrograde adverse effects, Feasibility Studies, Female, Humans, Male, Middle Aged, Operative Time, Patient Care Team, Postoperative Complications epidemiology, Postoperative Complications etiology, Reoperation adverse effects, Retrospective Studies, Single-Balloon Enteroscopy adverse effects, Anastomosis, Roux-en-Y adverse effects, Biliary Tract Diseases surgery, Cholangiopancreatography, Endoscopic Retrograde methods, Pancreatic Diseases surgery, Reoperation methods, Single-Balloon Enteroscopy methods
- Abstract
Background: Bilioenteric Roux-en-Y anastomosis is one of the most complicated approaches for reconstructing the gastrointestinal tract, and endoscopic retrograde cholangiopancreatography (ERCP) is technically challenging in patients after bilioenteric Roux-en-Y anastomosis. The optimal endoscopic strategies for such cases remain unknown., Aim: To explore the feasibility and effectiveness of single balloon enteroscopy-assisted (SBE-assisted) therapeutic ERCP in patients after bilioenteric Roux-en-Y anastomosis based on multi-disciplinary collaboration between endoscopists and surgeons as well as report the experience from China., Methods: This is a single center retrospective study. All of the SBE-assisted therapeutic ERCP procedures were performed by the collaboration between endoscopists and surgeons. The operation time, success rate, and complication rate were calculated., Results: Forty-six patients received a total of 64 SBE-assisted therapeutic ERCP procedures, with successful scope intubation in 60 (93.8%) cases and successful diagnosis in 59 (92.2%). All successfully diagnosed cases received successful therapy. None of the cases had perforation or bleeding during or after operation, and no post-ERCP pancreatitis occurred., Conclusion: Based on multi-disciplinary collaboration, SBE-assisted therapeutic ERCP in patients after bilioenteric Roux-en-Y anastomosis is relatively safe and effective and has a high success rate., Competing Interests: Conflict-of-interest statement: The authors declare no conflict of interest. The authors declare that they have nothing to disclose., (©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.)
- Published
- 2019
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20. Detection of Plasma EGFR Mutations in NSCLC Patients with a Validated ddPCR Lung cfDNA Assay.
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Guo QM, Wang L, Yu WJ, Qiao LH, Zhao MN, Hu XM, Sun YM, Ni S, Xu YH, and Lou JT
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Purpose : The clinical utility of cell-free DNA (cfDNA) to assess EGFR mutations is increasing. However, there are limited studies determining their clinical validity and utility. The value of cfDNA assays in cancer management remains controversial. Methods : In this study, we first evaluated the analytical performance of the ddPCR Lung cfDNA Assay. We next analyzed the concordance of the results with tissue amplification refractory mutation system PCR (ARMS-PCR) and plasma next-generation sequencing (NGS) genotyping. Finally, we assessed its clinical utility by exploring the association of cfDNA EGFR mutations with metastatic sites and the efficacy of EGFR-TKIs treatment. Results : The ddPCR Lung cfDNA Assay demonstrated a limit of blank of 1 droplet per reaction, an analytical specificity of 100%, and detection limit of 0.05%, 0.05%, and 0.1% for E746_A750del , L858R , and T790M , respectively. With tissue ARMS-PCR as a standard for comparison, the clinical sensitivity and specificity of ddPCR were 62.5% (15/24) and 100% (82/82) for E746_A750del , and 75.0% (15/20) and 94.2% (81/86) for L858R , respectively. The ddPCR showed high concordance with NGS in determining cfDNA EGFR mutations. Patients with bone and/or brain metastasis showed a higher detection rate and mutant abundance of cfDNA EGFR mutations compared to those with other sites of metastasis. Moreover, EGFR-TKIs treatment was effective in patients with sensitive EGFR mutations in either plasma cfDNA or tumor tissue-derived DNA. Conclusions : We validated in this study that the ddPCR Lung cfDNA Assay is reliable for detection of EGFR mutations in lung cancers, in terms of analytical performance, clinical validity and utility., Competing Interests: Competing Interests: The authors have declared that no competing interest exists.
- Published
- 2019
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21. [Endoscopicretrograde cholangio-pancreatography management of long-term complications after pancreaticoduodenectomy].
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Wu WW, Zhang WJ, Gu J, Zhao MN, Weng H, Weng MZ, Zhang Y, Qu CY, Xu LM, Liu YB, and Wang XF
- Subjects
- Adolescent, Adult, Aged, Anastomosis, Surgical, Biliary Tract Surgical Procedures, Female, Gallstones, Humans, Male, Middle Aged, Young Adult, Cholangiopancreatography, Endoscopic Retrograde, Pancreaticoduodenectomy adverse effects, Postoperative Complications therapy
- Abstract
Objective: To investigate the feasibility and effectiveness of endoscopicretrograde cholangio-pancreatography(ERCP)in the management of long-term complications after pancreaticoduodenectomy. Methods: From January 2009 to July 2018, the clinical data of 62 patients with biliary or pancreatic long-term complications after pancreatoduodenectomy were reviewed at Department of General Surgery, and the corresponding ERCP were carried out in the multi-disciplinary cooperation.There were 39 males and 24 females.The age was 56.5 years(aging from 13 to 76 years). The time of treatment was 3 months to 20 years after pancreatoduodenectomy.The long-term biliopancreatic complications after pancreatoduodenectomy included 51 cases of biliary calculi, 42 cases of bilioenteric anastomotic stenosis with proximal bile duct dilatation, and 11 cases of pancreaticointestinal anastomosis stenosis with distal pancreatic duct dilatation.All patients received conventional duodenoscopy or single-balloon enteroscopy assisted ERCP under general anesthesia. Results: A total of 95 ERCP were performed in 62 patients, averaging 1.5 times per case.The long-term complications of cholangiopancreatic after pancreatoduodenectomy(ERCP indications) included 56 times of bile duct stones(58.9%), 45 times of bilioenteric anastomatic stricture(47.4%), 11 times of recurrent pancreatitis(11.6%), 6 cases(6.3%) of bilioenteric anastomatic foreign body, 3 times of intrahepatic bile duct stenosis(3.2%). Among the 95 times, 82 times(86.3%) achieved endoscopic endoscopy, 76 times(80.0%) were diagnosed successfully, and 72 times(75.8%) were successfully treated with ERCP.Small intestinal perforation occurred in 1 patient undergoing duodenoscopy, and then healed by surgical repair. Conclusion: Multi-disciplinary collaboration of ERCP is safe and effective in the treatment of long-term complications after pancreaticoduodenectomy, but the long-term effect still needs further clinical follow-up.
- Published
- 2018
- Full Text
- View/download PDF
22. Correction to Copper-Promoted 6- endo-trig Cyclization of β,γ-Unsaturated Hydrazones for the Synthesis of 1,6-Dihydropyridazines.
- Author
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Guo YQ, Zhao MN, Ren ZH, and Guan ZH
- Published
- 2018
- Full Text
- View/download PDF
23. Modular 2,3-diaryl-2H-azirine synthesis from ketoxime acetates via Cs 2 CO 3 -mediated cyclization.
- Author
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Zhao MN, Zhang W, Wang XC, Zhang Y, Yang DS, and Guan ZH
- Abstract
A modular 2H-azirine synthesis from ketoxime acetates via Cs2CO3-mediated cyclization has been developed. The reaction utilizes easily available starting materials and provides a general synthetic route to 2,3-diaryl-2H-azirines in good to excellent yields under mild conditions, which is complementary to the conventional approaches for the synthesis of 2H-azirines. A gram-scale reaction was performed to demonstrate the scale-up applicability of this synthetic method. Importantly, 2H-azirines can be efficiently converted to various azaheterocycles.
- Published
- 2018
- Full Text
- View/download PDF
24. Copper-Promoted 6- endo-trig Cyclization of β,γ-Unsaturated Hydrazones for the Synthesis of 1,6-Dihydropyridazines.
- Author
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Guo YQ, Zhao MN, Ren ZH, and Guan ZH
- Abstract
A novel and efficient strategy for the synthesis of 1,6-dihydropyridazines via copper-promoted 6- endo-trig cyclization of readily available β,γ-unsaturated hydrazones have been developed. A series of 1,6-dihydropyridazines have been synthesized by this method with good yields, high functional group tolerance, and remarkable regioselectivity under mild conditions. Importantly, the 1,6-dihydropyridazines can be efficiently converted to biologically important pyridazines in the presence of NaOH.
- Published
- 2018
- Full Text
- View/download PDF
25. Copper-Catalyzed Oxidative Cyclization/1,2-Amino Migration Cascade Reaction.
- Author
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Zhao MN, Zhang ZJ, Ren ZH, Yang DS, and Guan ZH
- Abstract
A novel and efficient copper-catalyzed tandem oxidative cyclization/1,2-amino migration of readily available enamino esters for the synthesis of substituted pyrroles has been developed. In this reaction, one C-N bond was cleaved, and two new C-N bonds and one C(sp
2 )-C(sp2 ) bond were constructed in one pot. This catalytic system has the obvious advantages of mild reaction conditions and the use of oxygen as the oxidant. The reaction tolerates a wide range of functional groups and is a reliable method for the straightforward synthesis of valuable aminomethyl-substituted pyrroles in good yields.- Published
- 2018
- Full Text
- View/download PDF
26. Iron-Catalyzed Radical Cycloaddition of 2H-Azirines and Enamides for the Synthesis of Pyrroles.
- Author
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Zhao MN, Ren ZH, Yang DS, and Guan ZH
- Abstract
A novel and efficient Fe-catalyzed radical cycloaddition of 2H-azirines and enamides for the synthesis of substituted pyrroles has been developed. The radical cycloaddition reaction proceeded through a conceptually new Fe(II)-catalyzed homolytic cleavage of C-N bond of 2H-azirines sequential radical cyclization with enamides. The reaction used readily available starting materials, tolerated various functional groups, and afforded valuable triaryl-substituted pyrroles in good to high yields under mild reaction conditions.
- Published
- 2018
- Full Text
- View/download PDF
27. Effects of a Nurse-Led Phone Follow-up Education Program Based on the Self-efficacy Among Patients With Cardiovascular Disease.
- Author
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Zhou Y, Liao J, Feng F, Ji M, Zhao C, and Wang X
- Subjects
- Adult, Aged, Aged, 80 and over, China, Female, Follow-Up Studies, Goals, Humans, Male, Middle Aged, Telemedicine, Young Adult, Cardiovascular Diseases prevention & control, Cardiovascular Diseases psychology, Patient Education as Topic, Practice Patterns, Nurses', Self Efficacy, Telephone
- Abstract
Background: The integration of self-efficacy (SE) theory within a nurse-led telephone follow-up education program (NP-FEP) has not been extensively evaluated for patients with cardiovascular disease., Objectives: The aim of this study is to determine the effectiveness of an NP-FEP in improving SE (primary outcome) and achieving goals related to cardiovascular risk (secondary outcome) for patients with cardiovascular disease., Methods: In June and July 2013, a total of 403 patients with cardiovascular disease in Shanghai were randomized into the intervention and control groups. Personalized end goals were established for improving cardiovascular risk for each patient. The control group received conventional follow-up education, whereas the intervention group was contacted by telephone 11 times over a 6-month period with staged goals developed based on SE theory. Self-efficacy scores and goals for reducing cardiovascular risk were assessed at baseline, at the end of the 6-month intervention, and 12 months after enrollment., Results: The SE scores in both groups increased at 6 months and decreased slightly at 12 months. The baseline SE scores were similar between the groups (P > .05), but the average SE scores were increased more for the intervention group than for the control group at 6 (P < .05) and 12 (P < .05) months. In addition, the final goal achievement rates for the intervention group were significantly higher than for the control group (P < .05). The difference between the 2 groups was reflected by differences in cardiac disease risk factors defined by the World Health Organization., Conclusion: The NP-FEP improved SE and facilitated achievement of goals related to risk factors in patients with cardiovascular disease for at least 1 year.
- Published
- 2018
- Full Text
- View/download PDF
28. Base-mediated formal [3+2] cycloaddition of β,γ-alkenyl esters and p-TsN 3 for the synthesis of pyrazoles.
- Author
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Zhao MN, Zhang MN, Ren ZH, Wang YY, and Guan ZH
- Abstract
A novel 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU)-mediated formal cycloaddition of β,γ-alkenyl esters with p-tolylsulfonylazide (p-TsN
3 ) for the synthesis of 3,5-disubstituted pyrazoles has been developed. The reaction proceeded through diazotization of β,γ-alkenyl esters sequential with thermodynamic cyclization. p-Tolylsulfonylazide played a role as a source of two-nitrogen synthons. The reaction employs readily available starting materials, tolerates a wide range of functional groups, and proceeds under mild conditions., (Copyright © 2017 Science China Press. Published by Elsevier B.V. All rights reserved.)- Published
- 2017
- Full Text
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29. Copper-catalyzed radical coupling of 1,3-dicarbonyl compounds with terminal alkenes for the synthesis of tetracarbonyl compounds.
- Author
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Zhang MN, Zhao MN, Chen M, Ren ZH, Wang YY, and Guan ZH
- Abstract
A novel and efficient copper-catalyzed radical cross-coupling of 1,3-dicarbonyl compounds with terminal alkenes for the synthesis of tetracarbonyl compounds with a quaternary carbon atom has been developed. Mechanistically, this transformation involves the construction of two C-C bonds and two C[double bond, length as m-dash]O bonds in a one-pot process. The reaction tolerates a wide range of functional groups and proceeds under mild conditions.
- Published
- 2016
- Full Text
- View/download PDF
30. Iron-Catalyzed Cyclization of Ketoxime Carboxylates and Tertiary Anilines for the Synthesis of Pyridines.
- Author
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Zhao MN, Ren ZH, Yu L, Wang YY, and Guan ZH
- Abstract
A novel and efficient iron-catalyzed cyclization of ketoxime carboxylates and N,N-dialkylanilines for the modular synthesis of diverse pyridines was developed. The reaction was initiated by Fe-catalyzed N-O bond cleavage of ketoxime carboxylates in the presence of tertiary anilines. The methylene carbon on N,N-dialkylanilines functioned as a source of one-carbon synthon in the reaction. The reaction used readily available starting materials, tolerated various functional groups, and afforded 2,4-disubstituted and 2,4,6-trisubstituted pyridines in good to high yields under mild conditions.
- Published
- 2016
- Full Text
- View/download PDF
31. Use of the Conventional Side-viewing Duodenoscope for Successful Endoscopic Retrograde Cholangiopancreatography in Postgastrectomy Patients.
- Author
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Wu WG, Mei JW, Zhao MN, Zhang WJ, Gu J, Tao YJ, Liu YB, and Wang XF
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Anastomosis, Roux-en-Y, Child, Cholangiopancreatography, Endoscopic Retrograde adverse effects, Cholestasis, Extrahepatic etiology, Constriction, Pathologic complications, Constriction, Pathologic therapy, Female, Gastroenterostomy, Humans, Male, Middle Aged, Retrospective Studies, Treatment Outcome, Young Adult, Cholangiopancreatography, Endoscopic Retrograde instrumentation, Cholestasis, Extrahepatic therapy, Duodenoscopes, Gallstones therapy, Gastrectomy
- Abstract
Objectives: The aim of this study was to evaluate the usefulness of the conventional side-viewing duodenoscope for successful endoscopic retrograde cholangiopancreatography (ERCP) in postgastrectomy patients., Methods: A total of 220 consecutive patients with bile duct stones or a distal common bile duct stricture who had previously undergone gastrectomy and were referred for ERCP were analyzed for the outcome of their ERCP. All ERCP procedures were performed using a conventional side-viewing duodenoscope. In patients who had undergone a Billroth II gastroenterostomy and total gastrectomy with Roux-en-Y reconstruction, we also used the procedure of retrieval balloon-assisted enterography., Results: The study group included 220 patients who had previously undergone gastrectomy (77 women and 143 men; mean age, 72.2 y; range, 11 to 93 y). The overall enterography success rate was 90.5% (199/220), and the diagnostic and ERCP success rates were both 88.6% (195/220). Endoscopy was unsuccessful in 21 patients who received Billroth II gastroenterostomy and Roux-en-Y reconstruction. After successful endoscopy, diagnostic and ERCP success was not achieved in 4 patients with Billroth II gastroenterostomy, with or without Braun anastomosis, due to cannulation failure. The procedure-related complication rate was 5.5% (12/220), including immediate bleeding (0.9%, 2/220), pancreatitis (4.1%, 9/220), and perforation (0.5%, 1/220). There were no procedure-related deaths., Conclusions: The side-viewing duodenoscope is a useful instrument for performing successful ERCP in patients postgastrectomy. In addition, retrieval balloon-assisted enterography may improve the enterography success rate in postgastrectomy patients with Billroth II and Roux-en-Y reconstruction.
- Published
- 2016
- Full Text
- View/download PDF
32. Bufalin induces cell cycle arrest and apoptosis in gallbladder carcinoma cells.
- Author
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Jiang L, Zhao MN, Liu TY, Wu XS, Weng H, Ding Q, Shu YJ, Bao RF, Li ML, Mu JS, Wu WG, Ding QC, Cao Y, Hu YP, Shen BY, Tan ZJ, and Liu YB
- Subjects
- Animals, Blotting, Western, Caspases metabolism, Cell Proliferation drug effects, Gallbladder Neoplasms drug therapy, Gallbladder Neoplasms metabolism, Humans, Male, Membrane Potential, Mitochondrial drug effects, Mice, Mice, Nude, Signal Transduction drug effects, Tumor Cells, Cultured, Xenograft Model Antitumor Assays, Antineoplastic Agents pharmacology, Apoptosis drug effects, Bufanolides pharmacology, Cell Cycle Checkpoints drug effects, Gallbladder Neoplasms pathology
- Abstract
Bufalin, a major digoxin-like immunoreactive component of the Chinese medicine Chan Su, has been shown to exert a potential for anticancer activity against various human cancer cell lines in vitro. However, no detailed studies have so far been reported on its action on human gallbladder carcinoma cells. In this study, bufalin remarkably inhibited growth in human gallbladder cancer cells by decreasing cell proliferation, inducing cell cycle arrest and apoptosis in a dose-dependent manner. Bufalin also disrupted the mitochondrial membrane potential (ΔΨm) and regulated the expression of cell cycle and apoptosis regulatory molecules. Activation of caspase-9 and the subsequent activation of caspase-3 indicated that bufalin may be inducing mitochondria apoptosis pathways. Intraperitoneal injection of bufalin for 3 weeks significantly inhibited the growth of gallbladder carcinoma (GBC-SD) xenografts in athymic nude mice. Taken together, the results indicate that bufalin may be a potential agent for the treatment of gallbladder cancer.
- Published
- 2014
- Full Text
- View/download PDF
33. Retrieval-balloon-assisted enterography for ERCP after Billroth II gastroenterostomy and Braun anastomosis.
- Author
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Wu WG, Zhang WJ, Gu J, Zhao MN, Zhuang M, Tao YJ, Liu YB, and Wang XF
- Subjects
- Aged, Aged, 80 and over, Cholangiopancreatography, Endoscopic Retrograde adverse effects, Cholangitis diagnosis, Cholangitis etiology, Duodenoscopes, Equipment Design, Female, Gallstones diagnosis, Gallstones etiology, Humans, Jaundice, Obstructive diagnosis, Jaundice, Obstructive etiology, Male, Middle Aged, Retrospective Studies, Treatment Outcome, Catheters, Cholangiopancreatography, Endoscopic Retrograde instrumentation, Cholangitis therapy, Gallstones therapy, Gastroenterostomy adverse effects, Jaundice, Obstructive therapy, Stomach Neoplasms surgery
- Abstract
Aim: To describe an optimal route to the Braun anastomosis including the use of retrieval-balloon-assisted enterography., Methods: Patients who received a Billroth II gastroenterostomy (n = 109) and a Billroth II gastroenterostomy with Braun anastomosis (n = 20) between January 2009 and May 2013 were analyzed in this study. Endoscopic retrograde cholangiopancreatography (ERCP) was performed under fluoroscopic control using a total length of 120 cm oblique-viewing duodenoscope with a 3.7-mm diameter working channel. For this procedure, we used a triple-lumen retrieval balloon catheter in which a 0.035-inch guidewire could be inserted into the "open-channel" guidewire lumen while the balloon could be simultaneously injected and inflated through the other 2 lumens., Results: For the patients with Billroth II gastroenterostomy and Braun anastomosis, successful access to the papilla was gained in 17 patients (85%) and there was therapeutic success in 16 patients (80%). One patient had afferent loop perforation, but postoperative bleeding did not occur. For Billroth II gastroenterostomy, there was failure in accessing the papilla in 15 patients (13.8%). ERCP was unsuccessful because of tumor infiltration (6 patients), a long afferent loop (9 patients), and cannulation failure (4 patients). The papilla was successfully accessed in 94 patients (86.2%), and there was therapeutic success in 90 patients (82.6%). Afferent loop perforation did not occur in any of these patients. One patient had hemorrhage 2 h after ERCP, which was successfully managed with conservative treatment., Conclusion: Retrieval-balloon-assisted enterography along an optimal route may improve the ERCP success rate after Billroth II gastroenterostomy and Braun anastomosis.
- Published
- 2014
- Full Text
- View/download PDF
34. Copper-catalyzed 5-endo-trig cyclization of ketoxime carboxylates: a facile synthesis of 2-arylpyrroles.
- Author
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Du W, Zhao MN, Ren ZH, Wang YY, and Guan ZH
- Subjects
- Carboxylic Acids metabolism, Catalysis, Copper metabolism, Cyclization, Oximes metabolism, Pyrroles metabolism, Carboxylic Acids chemistry, Copper chemistry, Oximes chemistry, Pyrroles chemical synthesis
- Abstract
A novel and facile copper-catalyzed 5-endo-trig cyclization of ketoxime carboxylates for the synthesis of 2-arylpyrroles has been developed. The reaction tolerates a range of functional groups and is a practical procedure for rapid synthesis of 2-arylpyrroles in high yields under mild conditions.
- Published
- 2014
- Full Text
- View/download PDF
35. Ruthenium-catalyzed cyclization of ketoxime acetates with DMF for synthesis of symmetrical pyridines.
- Author
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Zhao MN, Hui RR, Ren ZH, Wang YY, and Guan ZH
- Subjects
- Cyclization, Molecular Structure, Pyridines chemistry, Acetates chemistry, Dimethylformamide chemistry, Oximes chemistry, Pyridines chemical synthesis, Ruthenium chemistry
- Abstract
A novel ruthenium-catalyzed cyclization of ketoxime carboxylates with N,N-dimethylformamide (DMF) for the synthesis of tetrasubstituted symmetrical pyridines has been developed. A methyl carbon on DMF performed as a source of a one carbon synthon. And NaHSO3 plays a role in the reaction.
- Published
- 2014
- Full Text
- View/download PDF
36. Palladium-catalyzed carbonylation of o-iodoanilines for synthesis of isatoic anhydrides.
- Author
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Gao S, Chen M, Zhao MN, Du W, Ren ZH, Wang YY, and Guan ZH
- Abstract
A novel palladium-catalyzed oxidative double carbonylation of o-iodoanilines for the synthesis of isatoic anhydrides has been developed. The reaction employs readily available o-iodoanilines as the starting materials and proceeds under mild conditions. For extension, palladium-catalyzed oxidative carbonylation of anthranilic acids was developed for the synthesis of substituted isatoic anhydrides in high to excellent yields.
- Published
- 2014
- Full Text
- View/download PDF
37. Copper-promoted oxidative coupling of enamides and alkynes for the synthesis of substituted pyrroles.
- Author
-
Zhao MN, Ren ZH, Wang YY, and Guan ZH
- Subjects
- Catalysis, Oxidative Coupling, Pyrroles chemistry, Alkynes chemistry, Amides chemistry, Copper chemistry, Pyrroles chemical synthesis
- Abstract
An efficient copper-promoted oxidative coupling of enamides with alkynes has been developed for the synthesis of substituted pyrroles. The reaction proceeded through C-H and N-H bond functionalization of enamides under mild conditions., (Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2014
- Full Text
- View/download PDF
38. Pd-catalyzed oxidative coupling of enamides and alkynes for synthesis of substituted pyrroles.
- Author
-
Zhao MN, Ren ZH, Wang YY, and Guan ZH
- Subjects
- Catalysis, Cyclization, Indoles chemical synthesis, Molecular Structure, Oxidation-Reduction, Pyrroles chemistry, Stereoisomerism, Alkynes chemistry, Amides chemistry, Palladium chemistry, Pyrroles chemical synthesis
- Abstract
A novel and efficient palladium(II)-catalyzed alkenyl C-H activation oxidative annulation of enamides with alkynes for the synthesis of substituted pyrroles has been developed. The reaction tolerates a wide range of functional groups and is a reliable method for the synthesis of triaryl-substituted pyrroles in high yields.
- Published
- 2014
- Full Text
- View/download PDF
39. ERCP for patients who have undergone Billroth II gastroenterostomy and Braun anastomosis.
- Author
-
Wu WG, Gu J, Zhang WJ, Zhao MN, Zhuang M, Tao YJ, Liu YB, and Wang XF
- Subjects
- Aged, Aged, 80 and over, Cholangiopancreatography, Endoscopic Retrograde instrumentation, Duodenoscopes, Female, Humans, Male, Middle Aged, Cholangiopancreatography, Endoscopic Retrograde methods, Gastroenterostomy methods
- Abstract
Endoscopic retrograde cholangiopancreatography (ERCP) is efficacious in patients who have undergone Billroth II gastroenterostomies, but the success rate decreases in patients who also have experienced Braun anastomoses. There are currently no reports describing the preferred enterography route for cannulation in these patients. We first review the patient's previous surgery records, which most often indicate that the efferent loop is at the greater curvature of the stomach. We recommend extending the duodenoscope along the greater curvature of the stomach and then advancing it through the "lower entrance" at the site of the gastrojejunal anastomosis, along the efferent loop, and through the "middle entrance" at the site of the Braun anastomosis to reach the papilla of Vater. Ten patients who had each undergone Billroth II gastroenterostomy and Braun anastomosis between January 2009 and December 2011 were included in our study. The overall success rate of enterography was 90% for the patients who had undergone Billroth II gastroenterostomy and Braun anastomosis, and the therapeutic success rate was 80%. We believe that this enterography route for ERCP is optimal for a patient who has had Billroth II gastroenterostomy and Braun anastomosis and helps to increase the success rate of the procedure.
- Published
- 2014
- Full Text
- View/download PDF
40. Coupling of enamides with alkynes or arynes for synthesis of substituted pyridines and isoquinolines via amide activation.
- Author
-
Zhao MN, Ren ZH, Wang YY, and Guan ZH
- Subjects
- Alkynes chemical synthesis, Amides chemical synthesis, Catalysis, Isoquinolines chemistry, Pyridines chemistry, Alkynes chemistry, Amides chemistry, Copper chemistry, Isoquinolines chemical synthesis, Pyridines chemical synthesis
- Abstract
A novel and general procedure for Cu-catalyzed coupling of enamides with alkynes to synthesize substituted pyridines was developed. The chemistry was allowed to extend to the synthesis of substituted isoquinolines by coupling of enamides with arynes under transition-metal-free conditions.
- Published
- 2012
- Full Text
- View/download PDF
41. Cu(OAc)2/TFA-promoted formal [3 + 3] cycloaddition/oxidation of enamines and enones for synthesis of multisubstituted aromatic amines.
- Author
-
Li L, Zhao MN, Ren ZH, Li JL, and Guan ZH
- Subjects
- Amines chemistry, Crystallography, X-Ray, Cyclization, Models, Molecular, Molecular Structure, Oxidation-Reduction, Stereoisomerism, Amines chemical synthesis, Ketones chemistry, Organometallic Compounds chemistry, Trifluoroacetic Acid chemistry
- Abstract
New strategies for the oxidative cycloaddition of enones with enamines are developed. These cycloaddition reactions directly afford substituted aromatic amines, which are important in organic chemistry, in moderate to good yield. Cu(OAc)(2)/TFA is shown to be essential to achieve high reaction efficiency.
- Published
- 2012
- Full Text
- View/download PDF
Catalog
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