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156 results on '"Zhao,Yading"'

42. Ultrasound-Targeted Microbubble Disruption with Key Nanodroplets for Effective Ferroptosis in Triple-Negative Breast Cancer Using Animal Model

Author

Liu,Rui, Shi,Dandan, Guo,Lu, Xiao,Shan, Shang,Mengmeng, Sun,Xiao, Meng,Dong, Zhao,Yading, Wang,Xiaoxuan, Li,Jie, Liu,Rui, Shi,Dandan, Guo,Lu, Xiao,Shan, Shang,Mengmeng, Sun,Xiao, Meng,Dong, Zhao,Yading, Wang,Xiaoxuan, and Li,Jie

Abstract

Rui Liu, Dandan Shi, Lu Guo, Shan Xiao, Mengmeng Shang, Xiao Sun, Dong Meng, Yading Zhao, Xiaoxuan Wang, Jie Li Department of Ultrasound, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, People’s Republic of ChinaCorrespondence: Jie Li, Department of Ultrasound, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, People’s Republic of China, Email jieli@email.sdu.edu.cnIntroduction: Triple-negative breast cancer (TNBC) is known to be the most aggressive form of breast cancer. Due to its high recurrence and mortality rates, the treatment of TNBC is a significant challenge for the medical community. Besides, ferroptosis is an emerging regulatory cell death that may provide new insights into the treatment of TNBC. As a central inhibitor of the ferroptosis process, the selenoenzyme glutathione peroxidase 4 (GPX4) is its classical therapeutic target. However, inhibition of GPX4 expression is quite detrimental to normal tissues. Ultrasound contrast agents, as an emerging visualization precision treatment, may provide a solution to the existing problem.Methods: In this study, nanodroplets (NDs) carrying simvastatin (SIM) were constructed using the homogeneous/emulsification method. Then, the characterization of SIM-NDs was systematically evaluated. Meanwhile, in this study, the ability of SIM-NDs combined with ultrasound-targeted microbubble disruption (UTMD) to initiate ferroptosis and its respective mechanisms of ferroptosis induction were verified. Finally, the antitumor activity of SIM-NDs was investigated in vitro and in vivo using MDA-MB-231 cells and TNBC animal models.Results: SIM-NDs exhibited excellent pH- and ultrasound-responsive drug release and noticeable ultrasonographic imaging ability, also showing good biocompatibility and biosafety. UTMD could promote increased intracellular reactive oxygen species and consume intracellular glutathione. However, SIM-NDs were efficiently internalized into cells under ultrasound irradiation, fol

Published
2023

45. Additional file 1 of Dual-sensitive and highly biocompatible O-carboxymethyl chitosan nanodroplets for prostate tumor ultrasonic imaging and treatment

Author

Meng, Dong, Guo, Lu, Shi, Dandan, Sun, Xiao, Shang, Mengmeng, Xiao, Shan, Zhou, Xiaoying, Zhao, Yading, Wang, Xiaoxuan, and Li, Jie

Abstract

Additional file 1: Figure S1 (A) In vitro biocompatibility of O-CS-DOX NDs at varied concentrations for 24 h treatment. (B) In vitro biocompatibility of O-CS-DOX NDs at varied concentrations for 48 h treatment.

Published
2023
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46. Additional file 1 of Ultrasound targeted microbubble destruction-triggered nitric oxide release via nanoscale ultrasound contrast agent for sensitizing chemoimmunotherapy

Author

Zhao, Yading, Shi, Dandan, Guo, Lu, Shang, Mengmeng, Sun, Xiao, Meng, Dong, Xiao, Shan, Wang, Xiaoxuan, and Li, Jie

Abstract

Additional file 1: Figure S1. A Tumor volume change per mouse at various treatments. B HE staining in main organs after the treatments. Scale bar: 100 µm. n=5. Figure S2. NO concentration after being triggered by ultrasound irradiation. n=3. Figure S3. A Fluorescent images of Hepa1-6 cells treated with DiO-labeled NDs under different pH values at 12, 24 h. NDs labeled with DiO appeared green dots, and cell nuclei counterstained by Hoechst were blue. Scale bar: 50 µm. B Quantitative histogram of FCM of Hepa1-6 cells treated with DiO-labeled NDs under different pH values at 12, 24 h. n=3.

Published
2023
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49. A sludge and modified rice husk ash-based geopolymer: synthesis and characterization analysis

Author

Zhao Yading, Wen Nana, Zhenyun Yu, and Minghao Liu

Subjects
Toxicity characteristic leaching procedure, Materials science, Renewable Energy, Sustainability and the Environment, 020209 energy, Strategy and Management, 05 social sciences, Alkalinity, Sorption, 02 engineering and technology, Raw material, Microstructure, Husk, Industrial and Manufacturing Engineering, Geopolymer, Compressive strength, Chemical engineering, 050501 criminology, 0202 electrical engineering, electronic engineering, information engineering, 0505 law, General Environmental Science
Abstract

There are many studies on the system of sludge and rice husk ash applied as the construction materials, but in most cases, the sludge needs to be incinerated, which induced the environmental contamination. This research investigated the viability of a novel geopolymer derived from non-calcined sludge and modified rice husk ash blend. The variable factors such as rice husk ash/sludge ratio, liquid alkaline activator/solid ratio and alkalinity on mechanical properties were studied, and the optimum mix ratio was then determined. The results indicate that compressive strength first increases and then decreases with the increase of the ratio of modified rice husk ash to dried sludge (RHA/DS). And at the RHA/DS ratio of 0.35 and liquid to solid ratio of 1.2, the geopolymer got the highest compressive strength. Mercury intrusion porosimetry (MIP) and X-ray diffraction (XRD) demonstrate that the decrease of the number of macropores (larger than 200 nm) improve the internal microstructure and amorphous phase with some crystalline as fillers. And the same results are confirmed by scanning electron microscope (SEM) analysis. Toxicity characteristic leaching procedure (TCLP) shows the new geopolymers have good environmental performance, with good sorption properties for heavy metals. However, relatively low mechanical properties, uncertainty in raw materials’ chemical composition and in long-term performance as well as durability, are a principal inhibition to use this material immediately.

Published
2019

50. Echogenic, Ultrasound-Sensitive Chitosan Nanodroplets for Spatiotemporally Controlled DKK-2 Gene Delivery to Prostate Cancer Cells

Author

Liu,Xinxin, Shi,Dandan, Guo,Lu, Zhou,Xiaoying, Shang,Mengmeng, Sun,Xiao, Meng,Dong, Zhao,Yading, Li,Jie, Liu,Xinxin, Shi,Dandan, Guo,Lu, Zhou,Xiaoying, Shang,Mengmeng, Sun,Xiao, Meng,Dong, Zhao,Yading, and Li,Jie

Abstract

Xinxin Liu, Dandan Shi, Lu Guo, Xiaoying Zhou, Mengmeng Shang, Xiao Sun, Dong Meng, Yading Zhao, Jie Li Department of Ultrasound, Qilu Hospital of Shandong University, Jinan, People’s Republic of ChinaCorrespondence: Jie LiDepartment of Ultrasound, Qilu Hospital of Shandong University, Wenhua Western Road, Jinan 107#, People’s Republic of ChinaTel/Fax +86-531-82166101Email jieli301@163.comPurpose: To synthesize echogenic chitosan/perfluorohexane nanodroplets (CNDs) for DKK-2 gene delivering in a spatiotemporally controlled manner in vitro.Methods: The characteristics, contrast-enhanced ultrasound imaging, DNA binding and DNase protection capacity, DKK-2 gene transfection and effects on LNCaP cells of these CNDs were investigated.Results: The obtained CNDs showed positive surface charges and could attract the genetic cargo with negative surface charges to form nanocomplexes. Agarose gel electrophoresis confirmed binding of the CNDs and pDNA. DKK-2 pDNA-loaded CNDs, in combination with ultrasound, ruptured and released DKK-2 pDNA, entering LNCaP cells through nano-scale pores in the cell membrane, which further reduced the proliferation of LNCaP cells.Conclusion: These stable and safe CNDs may be a promising choice to achieve efficient ultrasound-mediated gene delivery to specific tissues in a spatiotemporally controlled manner.Keywords: nanodroplets, chitosan, DKK-2, ultrasonic gene transfer, prostate cancer

Published
2021

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