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Your search keyword '"Zhao, Zhi-meng"' showing total 19 results
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19 results on '"Zhao, Zhi-meng"'

8. LincRNA_XR209691.3 could promote Bombyx mori nucleopolyhedrovirus replication by interacting with BmHSP70.

Author

Lin, Su, Yin, Hao Tong, Zhao, Zhi Meng, Chen, Zi Kang, Zhou, Xue MIng, Zhang, Zheng Dong, Guo, Xi Jie, Zhao, Wei Guo, and Wu, Ping

Subjects
LINCRNA, SILKWORMS, NUCLEOPOLYHEDROVIRUSES, GENE expression, NUCLEOTIDES
Abstract

Long non‐coding RNAs (lncRNAs), a class of transcripts exceeding 200 nucleotides and lacking protein coding potential, have been proven to play important roles in viral infection and host immunity. Bombyx mori nucleopolyhedrovirus (BmNPV) is an important pathogen, which causes the silkworm disease and leads to a huge challenge to the sericultural industry. At present, research on the roles of insect lncRNAs in host‐virus interaction are relatively few. In this study, we explored the function of lincRNA_XR209691.3 that was significantly up‐regulated in the silkworm fat body upon BmNPV infection. Firstly, the subcellular localization experiment confirmed that lincRNA_XR209691.3 was present in both the nucleus and cytoplasm. Enhancing the expression of lincRNA_XR209691.3 in BmN cells could promote the proliferation of BmNPV, while inhibition of lincRNA_XR209691.3 by RNA interference suppresses the proliferation of BmNPV. Combining RNA pull‐down and mass spectrometry, we identified the host and BmNPV proteins that could interact with lincRNA_XR209691.3. Next, by using truncation experiment and RNA immunoprecipitation (RIP) assay, it was found that lincRNA_XR209691.3 could bind to the Actin domain of BmHSP70. Subsequently, overexpression of lncRNA_XR209691.3 in BmN cells promoted the expression of BmHSP70, while knockdown of BmHsp70 suppressed the replication of BmNPV. Based on the above results, it is speculated that lincRNA_XR209691.3 could promote the proliferation of BmNPV through interaction with BmHSP70, possibly by improving the stability of BmHSP70 and thereby enhancing the expression of BmHSP70. Our results shed light on the lncRNA function in insect‐pathogen interactions and provide a new clue to elucidate the molecular mechanism of BmNPV infection. [ABSTRACT FROM AUTHOR]

Published
2023
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9. Mixed-mode I-II mesoscale fracture behavior of concrete determined by the realistic aggregate numerical model

Author

Yu Jiangjiang, Si Xiaolong, Zhang Li, Ren Zhengyi, Zhao Zhi-meng, Zhang Wei, Tang Jiangqi, and Li Guodong

Subjects
Materials science, Computer simulation, 0211 other engineering and technologies, 020101 civil engineering, 02 engineering and technology, Building and Construction, Mechanics, Dissipation, 0201 civil engineering, Cracking, Properties of concrete, 021105 building & construction, Volume fraction, Shear stress, General Materials Science, Gradation, Mortar, Civil and Structural Engineering
Abstract

In this study, the aggregate information in concrete was extracted by Digital Image Processing (DIP) technology, and the two-dimension three-phase (coarse aggregate, mortar, and interface transition zone) meso-numerical model corresponding to the realistic aggregate distribution was established. Based on the model, considering the effects of coarse aggregate distribution, volume fraction and size on the fracture behavior, the fracture test and numerical simulation of four-point shear beams with notches under mixed-mode I-II stress were carried out. The results showed that the concrete meso-numerical model based on real aggregate can accurately reflect the meso-scale fracture process of concrete, and cracks always develop along the weak interface transition zone. It is the aggregate in the fracture trend zone that makes the fracture path tortuous, and the bending and shearing stress around the aggregate will change substantially. Moreover, the external force work of numerical model increases with the increase of aggregate volume fraction and maximum aggregate size, and the dissipated energy of coarse aggregate with small particle size increases with the rise in its proportion in gradation. Especially, the spatial distribution of the coarse aggregate determines the meso-mechanical properties of concrete and then affects the macro-mechanical behavior. To reduce the cracking risk of concrete, the volume fraction and gradation of aggregate should be kept within an appropriate range.

Published
2019
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10. Research and application of random aggregate model in determining the fracture behavior of four-point bending beam with notch

Author

Zhang Ming, Yu Jiangjiang, Jin Feng, Cao Peng, Li Guodong, and Zhao Zhi-meng

Subjects
Digital image correlation, Materials science, Aggregate (composite), business.industry, 0211 other engineering and technologies, 020101 civil engineering, 02 engineering and technology, Building and Construction, Bending, Structural engineering, Displacement (vector), 0201 civil engineering, Precast concrete, 021105 building & construction, Fracture (geology), General Materials Science, Point (geometry), business, Failure mode and effects analysis, Civil and Structural Engineering
Abstract

The fracture and damage behaviors of concrete under I–II mixed-mode loading are of great importance in determining the failure mode of concrete material and structure. In this paper, the four-point bending beams with different aggregate volume contents have been fabricated and tested to obtain the limited loading capacities, and the Digital Image Correlation (DIC) method was utilized for monitoring the local displacement above the notch during the loading procedure. Furthermore, based on the aggregate occupation and translation algorithm, a modified random aggregate model has been proposed for generating the four-point bending beam with precast notch, and the numerical simulations for the four-point bending beam with different aggregate volume contents and depths have been proceed.

Published
2019
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13. Cellular Lnc_209997 suppresses Bombyx mori nucleopolyhedrovirus replication by targeting miR‐275‐5p in B. mori.

Author

Lin, Su, Zhang, Shao Lun, Yin, Hao Tong, Zhao, Zhi Meng, Chen, Zi Kang, Shen, Man Man, Zhang, Zheng Dong, Guo, Xi Jie, and Wu, Ping

Subjects
SILKWORMS, LINCRNA, NUCLEOPOLYHEDROVIRUSES, MOLECULAR interactions, NON-coding RNA
Abstract

Long non‐coding RNA (lncRNA) is a type of non‐coding RNA molecule, which exceeds 200 nucleotides in length and participates in the regulation of a variety of life activities. Recent studies showed that lncRNAs play important roles in viral infection and host immunity. At present, the researches on insect lncRNAs are relatively few. In this study, we found the expression of Lnc_209997 was significantly down‐regulated in silkworm fat body infected with Bombyx mori nucleopolyhedrosis virus (BmNPV). Inhibition of Lnc_209997 promoted BmNPV replication. Enhancing the expression of Lnc_209997 inhibited the proliferation of BmNPV. miR‐275‐5p was up‐regulated in silkworm fat body infected with BmNPV. Dual luciferase reporter gene system confirmed the interaction between Lnc_209997 and miR‐275‐5p. Over‐expression of Lnc_209997 inhibited the expression of miR‐275‐5p, while inhibition of Lnc_209997 enhanced the expression of miR‐275‐5p. Further, over‐expression of miR‐275‐5p can facilitate the replication of BmNPV. These results suggested that BmNPV could increase the expression of miR‐275‐5p by inhibiting cellular Lnc_209997 expression to promote their own proliferation. Our results are helpful for better understanding the role of lncRNAs in BmNPV infection, and provide insights into elucidating the molecular mechanism of interaction between Bombyx mori and virus. [ABSTRACT FROM AUTHOR]

Published
2022
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