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Objective: To investigate clinical significance of detections of fibrinogen (FIB), D-dimer (DD), antithrombin Ⅲ(AT-Ⅲ), protein C (PC) and protein S (PS) in plasma of patients with different degrees of cirrhosis. Methods: A total of 418 patients with cirrhosis treated in our hospital from October 2019 to June 2021 were enrolled as the observation group, including 138 patients with cirrhosis at the compensatory stage and 280 patients with cirrhosis at the decompensa-ted stage. Meanwhile, 115 healthy people who underwent physical examination during the same period were served as control group. The activities of FIB, DD, AT-Ⅲ, PC and PS were detected, and the differences in coagulation indictors between the cirrhosis compensatory group, the cirrhosis decompensated group and the normal control group were analyzed. Results: The activities of anticoagulant factors AT-Ⅲ, PC and PS in cirrhosis group were significantly decreased, than those with normal control group (P

In this study, a preparative high performance centrifugal partition chromatography (CPC) method for isolation and purification of the bioactive component astilbin from the crude extract ofSmilax glabrawas successfully established by usingn-hexane-ethyl acetate-methanol-water as the two-phase solvent system. The upper phase ofn-hexane-ethyl acetate-methanol-water (1:10:1:10, v/v) was used as the stationary phase of CPC. Under the optimum conditions, 88 mg of astilbin at 98.5% purity, as determined by HPLC, was yielded from 500 mg of the crude extract in a single CPC separation. The peak fraction of CPC was identified by1H NMR and13C NMR. [ABSTRACT FROM AUTHOR]