Search

Simple Summary: In the duck breeding process, the rapid and accurate identification of gender is currently a key challenge. This study proposes a method based on sound signal discrimination to address this issue. The vocalizations of one-day-old male and female ducklings were extracted and the signal features were obtained through professional processing. Classification models were established using a backpropagation neural network (BPNN), a deep neural network (DNN), and a convolutional neural network (CNN). In total, 70% of the dataset was used for training, and 30% was used for testing. The test results showed that the BPNN model had the highest accuracy rate of 93.33% in identifying male ducks, while the CNN model had the highest accuracy rate of 95% in identifying female ducks. From the perspective of overall model performance, the CNN model performed the best, with the highest accuracy rate and F1-score of 84.15% and 84.32%, respectively. This study can provide a technical reference and a foundation for future voice recognition technologies for different poultry breeds and applications. Gender recognition is an important part of the duck industry. Currently, the gender identification of ducks mainly relies on manual labor, which is highly labor-intensive. This study aims to propose a novel method for distinguishing between males and females based on the characteristic sound parameters for day-old ducks. The effective data from the sounds of day-old ducks were recorded and extracted using the endpoint detection method. The 12-dimensional Mel-frequency cepstral coefficients (MFCCs) with first-order and second-order difference coefficients in the effective sound signals of the ducks were calculated, and a total of 36-dimensional feature vectors were obtained. These data were used as input information to train three classification models, include a backpropagation neural network (BPNN), a deep neural network (DNN), and a convolutional neural network (CNN). The training results show that the accuracies of the BPNN, DNN, and CNN were 83.87%, 83.94%, and 84.15%, respectively, and that the three classification models could identify the sounds of male and female ducks. The prediction results showed that the prediction accuracies of the BPNN, DNN, and CNN were 93.33%, 91.67%, and 95.0%, respectively, which shows that the scheme for distinguishing between male and female ducks via sound had high accuracy. Moreover, the CNN demonstrated the best recognition effect. The method proposed in this study can provide some support for developing an efficient technique for gender identification in duck production. [ABSTRACT FROM AUTHOR]

Salted duck egg yolk, a key ingredient in various specialty foods in China, frequently contains broken eggshell fragments embedded in the yolk due to high-speed shell-breaking processes, which pose significant food safety risks. This paper presents an online detection method, YOLOv7-SEY-DeepSORT (salted egg yolk, SEY), designed to integrate an enhanced YOLOv7 with DeepSORT for real-time and accurate identification of salted egg yolks with impurities on production lines. The proposed method utilizes YOLOv7 as the core network, incorporating multiple Coordinate Attention (CA) modules in its Neck section to enhance the extraction of subtle eggshell impurities. To address the impact of imbalanced sample proportions on detection accuracy, the Focal-EIoU loss function is employed, adaptively adjusting bounding box loss values to ensure precise localization of yolks with impurities in images. The backbone network is replaced with the lightweight MobileOne neural network to reduce model parameters and improve real-time detection performance. DeepSORT is used for matching and tracking yolk targets across frames, accommodating rotational variations. Experimental results demonstrate that YOLOv7-SEY-DeepSORT achieves a mean average precision (mAP) of 0.931, reflecting a 0.53% improvement over the original YOLOv7. The method also shows enhanced tracking performance, with Multiple Object Tracking Accuracy (MOTA) and Multiple Object Tracking Precision (MOTP) scores of 87.9% and 73.8%, respectively, representing increases of 17.0% and 9.8% over SORT and 2.9% and 4.7% over Tracktor. Overall, the proposed method balances high detection accuracy with real-time performance, surpassing other mainstream object detection methods in comprehensive performance. Thus, it provides a robust solution for the rapid and accurate detection of defective salted egg yolks and offers a technical foundation and reference for future research on the automated and safe processing of egg products. [ABSTRACT FROM AUTHOR]

Simple Summary: Efficient breeding of meat ducks using three-dimensional and multi-layer cages is a novel approach being actively explored in China. In this process, timely and accurate detection of abnormal situations among ducks is crucial for optimizing and refining the cage-rearing system, and ensuring animal health and welfare. This study focused on the overturned and dead status of cage-reared ducks using YOLOv8 as the basic network. By introducing GAM and Wise-IoU loss functions, we proposed an abnormal-situation recognition method for cage-reared ducks based on YOLOv8-ACRD. Building on this, we refined the identification of key body parts of cage-reared ducks, focusing on six key points: head, beak, chest, tail, left foot, and right foot. This resulted in the development of an abnormal posture estimation model for cage-reared ducks, based on HRNet-48. Furthermore, through multiple tests and comparative verification experiments, it was confirmed that the proposed method exhibited high detection accuracy, generalization ability, and robust comprehensive performance. The method proposed in this study for perceiving abnormal situations in cage-reared ducks not only provides foundational information for the progress and improvement of the meat duck cage-reared system but also offers technological references for the intelligent breeding of other cage-reared poultry. Overturning and death are common abnormalities in cage-reared ducks. To achieve timely and accurate detection, this study focused on 10-day-old cage-reared ducks, which are prone to these conditions, and established prior data on such situations. Using the original YOLOv8 as the base network, multiple GAM attention mechanisms were embedded into the feature fusion part (neck) to enhance the network's focus on the abnormal regions in images of cage-reared ducks. Additionally, the Wise-IoU loss function replaced the CIoU loss function by employing a dynamic non-monotonic focusing mechanism to balance the data samples and mitigate excessive penalties from geometric parameters in the model. The image brightness was adjusted by factors of 0.85 and 1.25, and mainstream object-detection algorithms were adopted to test and compare the generalization and performance of the proposed method. Based on six key points around the head, beak, chest, tail, left foot, and right foot of cage-reared ducks, the body structure of the abnormal ducks was refined. Accurate estimation of the overturning and dead postures was achieved using the HRNet-48. The results demonstrated that the proposed method accurately recognized these states, achieving a mean Average Precision (mAP) value of 0.924, which was 1.65% higher than that of the original YOLOv8. The method effectively addressed the recognition interference caused by lighting differences, and exhibited an excellent generalization ability and comprehensive detection performance. Furthermore, the proposed abnormal cage-reared duck pose-estimation model achieved an Object Key point Similarity (OKS) value of 0.921, with a single-frame processing time of 0.528 s, accurately detecting multiple key points of the abnormal cage-reared duck bodies and generating correct posture expressions. [ABSTRACT FROM AUTHOR]

E2-Spy (abbreviated as ES) plays a vital role as a component in the Bacterial-Like Particles (BLPs) vaccine against classical swine fever virus (CSFV). This vaccine demonstrates remarkable immunoprotection, highlighting the importance of augmenting ES production in the development of CSFV subunit vaccines. In this study, a Pichia pastoris strain capable of high-yield secretory production of ES was developed through signal peptide engineering, gene dosage optimization and co-expression of molecular chaperones. Initially, a hybrid signal peptide cSP3 was engineered, leading to a 3.38-fold increase in ES production when compared to the control strain 1-α-ES. Subsequently, cSP3 was evaluated for its expression efficiency alongside different commonly used signal peptides under multicopy conditions. SDS-PAGE analysis revealed that 2-αd14-ES exhibited the highest ES production, displaying a 4.38-fold increase in comparison to 1-α-ES. Afterwards, SSA1, YDJ1, BIP, LHS1, and their combinations were integrated into 2-αd14-ES, resulting in a 1.92-fold rise in ES production compared to 2-αd14-ES (equivalent to a 6.18-fold increase compared to 1-α-ES). The final yield of ES was evaluated as 168.3 mg/L through comparison with serially diluted BSA protein bands. [ABSTRACT FROM AUTHOR]

Automated body weight (BW) estimation is an important indicator to reflect the automation level of breeding, which can effectively reduce the damage to animals in the breeding process. In order to manage meat rabbits accurately, reduce the frequency of manual intervention, and improve the intelligent of meat rabbit breeding, this study constructed a meat rabbit weight estimation system to replace manual weighing. The system consists of a meat rabbit image acquisition robot and a weight estimation model. The robot stops at each cage in turn and takes a top view of the rabbit through an RGB camera. The images from the robot are automatically processed in the weight estimation model, which consists of the meat rabbit segmentation network based on improved Mask RCNN and the BW fitting network. Attention mechanism, PointRend algorithm, and improved activation function are proposed to improve the performance of Mask RCNN. Six morphological parameters (relative projected area, contour perimeter, body length, body width, skeleton length, and curvature) are extracted from the obtained mask, and are sent into the BW fitting network based on SVR-SSA-BPNN. The experiment shows that the system achieves a 4.3% relative error and 172.7 g average absolute error in BW estimation for 441 rabbits, while the meat rabbit segmentation network achieves a 99.1% mean average precision (mAP) and a 98.7% mean pixel accuracy (MPA). The system provides technical support for automatic BW estimation of meat rabbits in commercial breeding, which is helpful to promote precision breeding. [ABSTRACT FROM AUTHOR]

The cost assessment of bridge maintenance is a difficult topic to study, but it is critical for a bridge life cycle cost analysis. The maintenance costs sample database was established in this study according to actual engineering data, and a bridge maintenance cost prediction model was developed using a fully-connected artificial neural network (ANN) and convolutional neural network (CNN), respectively. First, eight main factors affecting maintenance costs were evaluated based on the random forest method, and the evaluation results were verified by an exploratory data analysis. The original data were then screened based on the isolation forest principle, and the recent gross domestic product (GDP) growth rate was used to illustrate the relationship between economic development and bridge maintenance costs. Finally, these two neural networks were used to establish maintenance cost prediction models, respectively. The results from the two models were compared and their prediction accuracies were analyzed. The prediction performance of the CNN model for bridge maintenance costs was found to be better than that of the traditional fully-connected ANN model. The results of this study will enhance the opportunity for bridge managers to balance lifecycle maintenance costs. [ABSTRACT FROM AUTHOR]