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Background: Klebsiella pneumoniae is a zoonotic opportunistic pathogen, and also one of the common pathogenic bacteria causing mink pneumonia. The aim of this study was to get a better understanding of the whole-genome of multi-drug resistant Klebsiella pneumoniae with K2 serotype in China. This study for the first time to analyze Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, resistance and virulence genes of Klebsiella pneumoniae in mink. Results: The isolate was Klebsiella pneumoniae with serotype K2 and ST6189 by PCR method. The string test was positive and showed high mucus phenotype. There was one plasmid with IncFIB replicons in the genome. The virulence factors including capsule, lipopolysaccharide, adhesin, iron uptake system, urease, secretory system, regulatory gene (rcsA, rcsB), determinants of pili adhesion, enolase and magnesium ion absorption related genes. The strain was multi-drug resistant. A total of 26 resistance genes, including beta-lactam, aminoglycosides, tetracycline, fluoroquinolones, sulfonamides, amide alcohols, macrolides, rifampicin, fosfomycin, vancomycin, diaminopyrimidines and polymyxin. Multidrug-resistant efflux protein AcrA, AcrB, TolC, were predicted in the strain. Conclusion: It was the first to identify that serotype K2 K. pneumonia with ST6189 isolated from mink in China. The finding indicated that hypervirulent and multi-drug resistant K. pneumoniae was exist in Chinese mink. The whole-genome of K. pneumoniae isolates have importance in mink farming practice. [ABSTRACT FROM AUTHOR]

Globally, metabolic dysfunction-associated steatotic liver disease (MASLD), previously termed nonalcoholic fatty liver disease (NAFLD), is one of the most common liver disorders and is strongly associated with copper deficiency. To explore the potential effects and mechanisms of Lactiplantibacillus plantarum LPJZ-658, copper deficiency combined with a high-sugar diet-induced MASLD mouse model was utilized in this study. We fed 40-week-old (middle-aged) male C57BL/6 mice a copper-deficient and high-sugar diet for 16 weeks (CuDS), with supplementary LPJZ-658 for the last 6 weeks (CuDS + LPJZ-658). In this study, we measured body weight, liver weight, and serum biochemical markers. Lipid accumulation, histology, lipidomics, and sphingolipid metabolism-related enzyme expression were investigated to analyze liver function. Untargeted metabolomics was used to analyze the serum and the composition and abundance of intestinal flora. In addition, the correlation between differential liver lipid profiles, serum metabolites, and gut flora at the genus level was measured. The results show that LPJZ-658 significantly improves abnormal liver function and hepatic steatosis. The lipidomics analyses and metabolic pathway analysis identified sphingolipid, retinol, and glycerophospholipid metabolism as the most relevant metabolic pathways that characterized liver lipid dysregulation in the CuDS group. Consistently, RT-qPCR analyses revealed that the enzymes catalyzing sphingolipid metabolism that were significantly upregulated in the CuDS group were downregulated by the LPJZ-658 treatment. In addition, the serum metabolomics results indicated that the linoleic acid, taurine and hypotaurine, and ascorbate and aldarate metabolism pathways were associated with CuDS-induced MASLD. Notably, we found that treatment with LPJZ-658 partially reversed the changes in the differential serum metabolites. Finally, LPJZ-658 effectively regulated intestinal flora abnormalities and was significantly correlated with differential hepatic lipid species and serum metabolites. In conclusion, we elucidated the function and potential mechanisms of LPJZ-658 in alleviating copper deficiency combined with sugar-induced middle-aged MASLD and hope this will provide possible treatment strategies for improving MASLD.

Objective: To investigate the effect of contrast water therapy on proprioception of the knee joint and indicators associated with fatigue in sprinters after high intensity training., Methods: A total of 40 sprinters were selected and divided into an observation group and a control group. The observation group got 14 minutes of contrast water therapy, while the control group took 14 minutes of sitting rest after training. The knee position sense, muscle force sense, joint reaction angle to release, knee joint function, and indictors associated with fatigue were evaluated before and after exercise at different time points., Results: At 24 h, 48 h, and 72 h after exercise, the active position sense and muscle force sense of the control group were significantly lower than those of the observation group (all P<0.05). At 48 h after exercise, the passive position sense of the control group was significantly lower than that of the observation group (all P<0.05). At 24 h and 48 h after exercise, the joint reaction angle to release of the control group was significantly greater than that of the observation group (all P<0.05). Additionally, the IKDC2000 and Lysholm scores after interference in the observation group were greater than those of the control group (all P<0.05). The indicators associated with fatigue after interference in the observation group were significantly lower than those of the control group (all P<0.05)., Conclusion: Contrast water therapy can effectively alleviate muscle force sense, promote muscle proprioception, improve knee joint function, and enhance recovery from fatigue., Competing Interests: None., (AJTR Copyright © 2024.)

Objective: To compare the differences in ankle joint parameters of basketball athletes between the forefoot and rearfoot landing and to investigate the injury mechanism of ankle joints in different landing modes., Methods: Twenty level II male basketball athletes were selected as subjects in this study. The landing movements of these athletes were assigned into a forefoot landing mode and a rearfoot landing mode. The former includes movements such as running emergency stop, two-leg jump and forefoot landing, while the latter includes actions such as running emergency stop, two-leg jump and rearfoot landing. The motion capture system and three-dimensional force measuring table were used for collecting the kinematic and dynamic data of the subjects., Results: The initial landing angles, including ankle dorsiflexion and medial ankle rotation of the forefoot were larger than those of the rearfoot (all P<0.05). Compared to those in the rearfoot landing mode, the forefoot landing exhibited a greater peak angle of ankle plantar flexion and ankle varus, as well as a smaller peak angle of ankle dorsiflexion and ankle internal rotation (all P<0.05). In comparison to the rearfoot landing mode, the forefoot landing showed a larger range of ankle varus and valgus, as well as a smaller range of ankle dorsiflexion and plantar flexion (all P<0.05). The ankle plantar flexion torque of forefoot landing was higher than that of rearfoot landing, while the peak ankle dorsiflexion torque of forefoot landing was smaller than that of rearfoot landing (all P<0.05). Compared to those in the rearfoot landing mode, the outward peak ground reaction force was smaller and the forward peak ground reaction was larger in forefoot landing mode (all P<0.05). No obvious differences were observed in other indicators between two landing modes., Conclusions: There are kinematic and dynamic differences between the forefoot and rearfoot landing. Forefoot landing may increase the risk of ankle injury during landing., Competing Interests: None., (AJTR Copyright © 2023.)