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4. Three-dimensional reconstruction of laryngeal cancer with whole organ serial immunohistochemical sections

Author

Liu Jie, J.-P. Jeannon, Pengfei He, Hui Zhang, Zhang Sanmei, Qian Bo, Jue Guo, Tian Jun, Yue Jia, Binquan Wang, Fu-Hui Huang, Li Le, Jin Rongxiu, Xiang Feng, Rui Guo, and Yangni Zhan

Subjects
0301 basic medicine, Male, Computer science, lcsh:Medicine, Context (language use), Iterative reconstruction, Article, Imaging, Immunological techniques, 03 medical and health sciences, 0302 clinical medicine, Imaging, Three-Dimensional, Microscopy, medicine, Image Processing, Computer-Assisted, Humans, Segmentation, lcsh:Science, Solid tumor, Laryngeal Neoplasms, Aged, Multidisciplinary, lcsh:R, 3D reconstruction, Cancer, Volume rendering, medicine.disease, Immunohistochemistry, Visualization, Data set, 030104 developmental biology, 030220 oncology & carcinogenesis, lcsh:Q, Cancer imaging, Lymph, Biomedical engineering
Abstract

Three-dimensional (3D) image reconstruction of tumors based on serial histological sectioning is one of the most powerful methods for accurate high-resolution visualization of tumor structures. However, 3D histological reconstruction of whole tumor has not yet been achieved. We established a high-resolution 3D model of molecular marked whole laryngeal cancer by optimizing the currently available techniques. A series of 5,388 HE stained or immunohistochemically stained whole light microscopic images (200 ×) were acquired (15.61 TB).The data set of block-face images (96.2 GB) was also captured. Direct volume rendering of serial 6.25 × light microscopy images did not demonstrate the major characteristics of the laryngeal cancer as expected. Based on fusion of two datasets, the accurate boundary of laryngeal tumor bulk was visualized in an anatomically realistic context. In the regions of interest, micro tumor structure, budding, cell proliferation and tumor lymph vessels were well represented in 3D after segmentation, which highlighted the advantages of 3D reconstruction of light microscopy images. In conclusion, generating 3D digital histopathological images of a whole solid tumor based on current technology is feasible. However, data mining strategy should be developed for complete utilization of the large amount of data generated.

Published
2020

7. Identification of Viruses in Patients With Postviral Olfactory Dysfunction by Multiplex Reverse‐Transcription Polymerase Chain Reaction.

Author

Tian, Jun, Pinto, Jayant M., Li, Li, Zhang, Sanmei, Sun, Zhifu, and Wei, Yongxiang

Abstract

Objectives/Hypothesis: To investigate causative viruses in patients with postviral olfactory disorders (PVOD). Study Design: Case‐control study. Methods: One hundred fifty‐one consecutive patients diagnosed with PVOD were enrolled, and samples from 38 patients who visited the doctor within 3 months of symptom onset were collected and analyzed. Thirty‐two individuals who underwent surgery for nasal septal deviation during the same time period were collected as the control group. The Sniffin' Sticks psychophysical olfactory test was used to evaluate olfactory function. Olfactory cleft specimens were collected using nasopharyngeal flocked swabs (COPAN FLOQSwabs). Eighteen viruses were tested for with the Luminex xTAG RVP FAST v2 Assay Kit. Results: Out of the 38 patients with PVOD, rhinoviruses were detected in 13 patients, and coronavirus OC43 was detected in one patient. The frequency of positive virus detection in the patients with anosmia was higher than in those with hyposmia (58.8% vs. 19.0%, P = 0.018). In control group, rhinovirus was identified in one patient (3.1%). Nasal obstruction was the most common symptom and was experienced by 71.0% of patients. Conclusions: Rhinovirus and coronavirus are more commonly identified in PVOD. Our methods represent an approach to screen for viruses that may be involved in PVOD. Level of Evidence: 4 Laryngoscope, 131:158–164, 2021 [ABSTRACT FROM AUTHOR]

Published
2021
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10. Aging and Physical Activity in the United States.

Author

LI Xiaohong, ZHOU Lanjun, and ZHANG Sanmei

Abstract

The article presents a study on aging and physical activities in the U.S. from the prospects of the local government, Federal government, and academic institutions.

Published
2013

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