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Objective: To examine the cellular distribution and the expression of CD200 and its receptor 1 (CD200R1) in human deciduas in first-trimester pregnant women with spontaneous early abortion (SEA) and normal pregnancy, and to explore their role in the etiology of SEA.Subjects and methods: Thirty-five women at 6-10-week gestation with SA and 30 women of similar gestational age with a healthy pregnancy were recruited. Expression of CD200 and CD200R1 in the deciduas was determined using immunohistochemistry, confocal laser scanning microscope, Western blot, and real-time PCR (RT-PCR).Results: The decidual stromal cells, glandular epithelial cells, and vessel endothelial cells during the first trimester of pregnancy express both CD200 and CD200R1 proteins. During this period, the expression of CD200 in glandular epithelial cells and vessel endothelial cells is significantly higher in normal pregnancy than that in women with SEA (0.3079 ± 0.0674 versus 0.2735 ± 0.0515; 0.4077 ± 0.1366 versus 0.3249 ± 0.0993); the expression of CD200R1 in stromal cells, decidual stromal cells, glandular epithelial cells is significantly higher during normal pregnancy than SEA (0.2574 ± 0.0588 versus 0.2292 ± 0.0415; 0.3617 ± 0.1046 versus 0.2804 ± 0.0640). Western blot analysis showed an approximately 44% decrease in CD200R1expression in decidua in the SEA versus the controls. Finally, in decidua, the expression of both CD200 protein and CD200R1 transcript are significantly higher in healthy first-trimester pregnancy than in SEA (CD200: 2.2089 ± 1.2754 versus 0.7241 ± 0.2143; CD200R1: 15.7843 ± 10.7085 versus 7.3381 ± 5.8529).Conclusions: Women with SEA have a lower level of CD200 and CD200R1 expression in deciduas compared with normal pregnant women suggesting that under physiological conditions, CD200 and CD200R1 expression by deciduas is important to prevent fetal loss ensure a successful pregnancy. [ABSTRACT FROM AUTHOR]

Hf-doped ZnO thin films with different Hf contents of target (HfZnO 0 ≤ x ≤ 10 at.%) were deposited on flexible PET substrates under different oxygen pressure using pulsed laser deposition. Microstructures, optical and electrical properties of the films were studied. The results show that the as-deposited HfZnO (0 ≤ x ≤ 5 at.%) films crystallized in ZnO hexagonal wurtzite structure with a highly preferred c-axis orientation. The films exhibited a transmission of higher than 80 % in the visible region. With increasing Hf content, the lattice constants increased; the morphology of the films deteriorated. The sheet resistance reached a minimum value of 16 Ω/□ when the doping level was about 0.5 at.%. [ABSTRACT FROM AUTHOR]

Aim: To investigate the correlation between interleukin-10(IL-10) and recurrent spontaneous abortion as well as the significance of them through researching the comparison of histological localization of IL-10, expression comparison of IL-10 mRNA in human chorionic villi of pregnant women with recurrent spontaneous abortion and normal abortion., Methods: To detect expression comparison of IL-10 mRNA and histological localization of IL-10 in human chorionic villi of 35 pregnant women with recurrent spontaneous abortion(experimental group) and 35 normal abortion women(control group) by reverse transcription-polymerase chain reaction and immunohistochemistry. To analysis expression of IL-10 in serum of the two groups by ELISA., Results: The detection of immunohistochemistry showed that there are expressions of IL-10 both in the two groups, the expressions of IL-10 in the experimental group were lower than the control group(P<0.05).The results of the RT-PCR showed that the expressions of IL-10 of experimental group were lower than the control group(P<0.05). ELISA analysis showed that the expressions of IL-10 of the experimental group were lower than the control group(P<0.05)., Conclusion: The results of detection of immunohistochemistry and the expression of mRNA of IL-10 in human chorionic villi as well as its expressions in serum of experimental group were lower than the control group. IL-10 would play an important role in maintaining normal pregnancy.

Objective: This study was conducted to detect the expression of laminin (LN) and laminin receptor (LN-R) in the specimens of hydatidiform mole (HM), invasive mole (IM) and choriocarcinoma(CC), respectively., Methods: The immunohistological staining method was used., Results: It was found that the level of the laminin expression was higher in the specimens of choriocarcinoma at more advanced stage., Conclusion: The results suggest that there may be relations between the invasion and metastasis of choriocacinoma cell and the malignant degree of this disease, chemotherapy has some effect on the expression of LN and LN-R in gestational trophablastic tumor (GTT), and that LN and LN-R may be of potential value in GTT treatment. However, referring whether LN and LN-R could be used as clinical prognosticators, further studies will be needed.