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As a new type of economic format, digital economy has three major characteristics: technical, innovative, energy-saving and environmentally friendly. Acting on various sectors of the national economy, it is beneficial for improving carbon emission efficiency and is of great significance for achieving China's two major goals of carbon peak and carbon neutrality. Firstly, theoretical analysis of the impact mechanism of digital economy on carbon emission efficiency, proposing research hypotheses on the direct effect, mediating effect, and spatial effect of digital economy on carbon emission efficiency. Secondly, based on panel data from 279 cities in China from 2011 to 2020, the econometric models are constructed to empirically analyze the direct, mediating, and spatial effects of digital economy on carbon emission efficiency. The results show that: 1) Digital economy can improve carbon emission efficiency; 2) The impact of digital economy on carbon emission efficiency has a "U"-shaped relationship, which is consistent with the "Environmental Kuznets Curve" hypothesis; 3) The impacts of digital economy on carbon emission efficiency exist in urban heterogeneity, specifically manifested as regional heterogeneity and urban scale heterogeneity; 4) Technological innovation is an important mediator for improving carbon emission efficiency in digital economy, and promoting technological innovation in digital economy can improve carbon emission efficiency; 5) Digital economy has spatial effect on carbon emission efficiency, which can improve the carbon emission efficiency of neighboring cities. Finally, based on the above results, suggestions are proposed from three aspects: promoting important industries and key areas for deep cultivation of carbon emission in digital economy, emphasizing regional balance in the development of digital economy, and strengthening regional cooperation in the development of digital economy, in order to continue to play a positive role in improving carbon emission efficiency through digital economy. [ABSTRACT FROM AUTHOR]

Improving the regenerative ability of senescent stem cells is a critical issue in combating aging. The destiny and function of senescent stem cells are controlled by the niche, including the physical architecture of the surface of the extracellular matrix (ECM). In this study, we explored the functions of TiO2 nanotube topography on mesenchymal stem cells (MSCs) under senescence, as well as its mechanical effects on senescence. First, we created different nanotube topographies on the titanium samples. Next, we cultured senescent mesenchymal stem cells (S-MSCs) on samples with various nanotube topographies to determine suitable parameters. We found nanotube with a diameter of 10 nm significantly alleviated the cellular senescence of S-MSCs and improved the osteogenic differentiation of S-MSCs in vitro. Using an ectopic periodontium regeneration model, we confirmed that specific nanotube topography could promote tissue regeneration of S-MSCs in vivo. Moreover, we demonstrated that nanotube topography activated YAP in S-MSCs and reformed nuclear-cytoskeletal morphology to inhibit senescence. Taken together, our study establishes a bridge linking between nano-topography, mechanics, and senescence, suggesting a potential strategy to improve tissue regeneration in aged individuals by providing optimized surface topography on biomaterials. [ABSTRACT FROM AUTHOR]

Apoptosis releases numerous apoptotic vesicles that regulate processes such as cell proliferation, immunity, and tissue regeneration and repair. Now, it has also emerged as an attractive candidate for biotherapeutics. However, apoptotic vesicles encompass a diverse range of subtypes, and it remains unclear which specific subtypes play a pivotal role. In this study, we successfully isolated different apoptotic vesicle subtypes based on their sizes and characterized them using NTA and TEM techniques, respectively. We compared the functional variances among the distinct subtypes of apoptotic vesicles in terms of stem cell proliferation, migration, and differentiation, as well as for endothelial cell and macrophage function, effectively identifying subtypes that exhibit discernible functional differences. ApoSEV (with diameter <1000 nm) promoted stem cell proliferation, migration, and multi‐potent differentiation, and accelerated skin wound healing of diabetes mouse model, while apoBD (with diameter >1000 nm) played the opposite effect on cell function and tissue regeneration. Lastly, employing protein analysis and gene sequencing techniques, we elucidated the intrinsic mechanisms underlying these differences between different subtypes of apoEVs. Collectively, this study identified that apoptotic vesicle subtypes possessed distinct bio‐functions in regulating stem cell function and behaviour and modulating tissue regeneration, which primarily attribute to the distinct profiling of protein and mRNA in different subtypes. This comprehensive analysis of specific subtypes of apoEVs would provide novel insights for potential therapeutic applications in cell biology and tissue regeneration. [ABSTRACT FROM AUTHOR]