1. Identification and functional analysis of acute myeloid leukemia susceptibility associated single nucleotide polymorphisms at non-protein coding regions of RUNX1.
- Author
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Xu, Xin, Ren, Xiuyu, Wang, Haiying, Zhao, Yao, Yi, Zhengjun, Wang, Kaifeng, Zhang, Shizhuang, Wang, Lin, Samuelson, David J., and Hu, Zhenbo
- Subjects
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ACUTE myeloid leukemia , *RUNX proteins , *DISEASE susceptibility , *SINGLE nucleotide polymorphisms , *GENETIC code - Abstract
Little is known about the susceptibility to acute myeloid leukemia. We aim to search non-protein coding regions of key hematopoiesis transcription factors for genetic variations associated with acute myeloid leukemia susceptibility. We genotyped SNPs ofRUNX1P1 promoter, P2 promoter, +23 enhancer, intron 5.2 enhancer,PU.1promoter,CEBPApromoter, andCEBPEpromoter from acute myeloid leukemia patients and healthy controls. Rs2249650 and rs2268276 atRUNX1intron 5.2 enhancer were found to be associated with acute myeloid leukemia susceptibility. Artificial reporters containing different rs2249650 and rs2268276 alleles showed differential activities in the K562 cell line, a human immortalized myeloid leukemia line. Rs2249650 contributes to reporter activities more than rs2268276. Gel shift assay is consistent with the luciferase assay. Supershift assay indicated that one potential binding protein was PU.1. To sum up, rs2268276 and especially rs2249650 may be qualified as new acute myeloid leukemia susceptibility-associated SNPs. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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