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Additional File 3: Fig. S3. GO enrichment analysis of the DEGs. (A) GO enrichment analysis of the DEGs in leaves of maize with added ��-PGA and control maize. (B) The more detailed classification of the terms of response to abiotic stimulus.

Additional file 1: Table S1. Basic characteristics of participants according to current carotid plaque status. China, 2017. Table S2. Additive interactions between shift work and insomnia on odds of carotid plaque. China, 2017. Figure S1. Relative risk with contributions from different exposure categories marked in additive interaction. U is the common reference category. Table S3. The Odds Ratio (OR) of carotid plaque in steelworkers with insomnia compare to non-insomnia. Multivariate logistic regression. China, 2017.

Additional File 8: Fig. S8. DEGs involved in ABA biosynthesis and ABA signaling pathway. Leaves from maize with added ��-PGA under drought stress was collected for RNA sequencing. The absolute values of log2 (CK+ ��-PGA/CK) ���1 and FDR < 0.001 were used as the criteria for DEGs. The color of the box represents up (red) and down (green)-regulated (CK+ ��-PGA/CK) genes, and the value in the box is the log2 (CK+ ��-PGA/CK) of the genes in the leaf (CK+ ��-PGA/CK) under drought stress.

Additional File 1: Fig. S1. Phenotypes of maize with added ��-PGA under drought stress. Phenotypes of maize with added different concentrations (0, 50 mg/L, 70 mg/L, 100 mg/L) of ��-PGA under the 7 d drought stress treatment and after re-watering for 1d. Bars=10 cm.

Additional File 9: Fig. S9. Species accumulation curves (boxplots) in rhizosphere soil of maize.

Additional File 7: Fig. S7. The promoter elements analysis of the DEGs (coded by nuclear DNA) involved in photosynthesis-antenna proteins. Different color squares represent different elements, and red squares represent ABA responsive elements.

Additional File 6: Fig. S6. The promoter elements analysis of the DEGs involved in photosynthesis. Different color squares represent different elements, and red squares represent ABA responsive elements.

Additional File 5: Fig. S5. Validation of DEG identified in RNA-seq by real-time RT-PCR. The left heatmap showed the log2 fold changes of the DEGs identified in RNA-seq. The right bar graph was the results by real-time RT-PCR. Log2 values (CK-PGA-D/CK-D) were used to generate the plot. Expression levels of genes were analyzed by real-time RT-PCR, fold changes in transcripts were calculated by 2-����Ct method with ZmTub as an internal control.

Additional File 4: Fig. S4. The DEGs involved in photosynthesis-antenna proteins. The DEGs involved in photosynthesis. The leaf from the maize with added ��-PGA under drought stress was collected for RNA sequencing. The absolute values of log2 (CK+��-PGA/CK) ���1 and FDR < 0.001 were used as the criteria for DEGs. The color of the box represents up (red) and down (green)-regulated (CK+ ��-PGA/CK) genes, and the value in the box is the log2 (CK+ ��-PGA/CK) of the genes in the leaf (CK+ ��-PGA/CK) under drought stress. The pattern of photosynthesis-antenna proteins comes from KEGG (http://www.genome.jp/kegg/).

Additional File 2: Fig. S2. Determination of the RWC and the solute potential. (A) The solute potential of the maize with and without added ��-PGA under drought stress treatment with 18% PEG6000 solution. (B) The relative water content (RWC) of the maize with and without added ��-PGA under drought stress treatment with 18% PEG6000 solution. Values are means �� sd. Bars represent means �� sd (n=5 repeats). Significant differences are indicated by asterisks (**, P ���0.01).