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9. Pyrotinib treatment on HER2-positive gastric cancer cells promotes the released exosomes to enhance endothelial cell progression, which can be counteracted by apatinib

Author

Gao,Zhengxing, Song,Chunqing, Li,Guangxin, Lin,Haishan, Lian,Xiangyao, Zhang,Ninggang, Cao,Bangwei, Gao,Zhengxing, Song,Chunqing, Li,Guangxin, Lin,Haishan, Lian,Xiangyao, Zhang,Ninggang, and Cao,Bangwei

Abstract

Zhengxing Gao,1 Chunqing Song,2 Guangxin Li,1 Haishan Lin,1 Xiangyao Lian,1 Ninggang Zhang,1 Bangwei Cao11Cancer Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, People’s Republic of China; 2Department of Oncology, Beijing Daxing District Hopeople’s Hospital, Capital Medical University, Beijing 102600, People’s Republic of ChinaAims: Pyrotinib is a newly developed irreversible pan-ErbB receptor tyrosine kinase inhibitor for treatment of human epidermal growth factor receptor 2 (HER2)-positive cancers, and clinic trials of pyrotinib in treatment of HER2-positive gastric cancer (GC) are underway. Exosomes are tiny vesicles secreted by cancer cells and take essential roles in the progression of carcinoma. Whether pyrotinib application has any effect on the cancer cell-released exosomes has not been studied. The aim of our work was to address if pyrotinib treatment impacts the effect of HER2-positive GC cell-derived exosomes on endothelial cell (EC) progression.Methods: Isolation of exosomes released by HER2-positive NCI-N87 and MKN45 lines after pyrotinib treatment was performed. Then, human umbilical vein endothelial cells (HUVECs) were incubated with different concentrations of exosomes to address their proliferation by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS). Effect of pyrotinib-treated exosomes at concentration of 10 μg/mL was compared to that without pyrotinib treatment over 96-hr time course. Transwell assay and wound-healing assay were carried out by incubating with exosomes released by NCI-N87 and MKN45 cells with/without pyrotinib treatment over 24-hr time course. The aforementioned experiments were done under same conditions in order to evaluate the combined effect of apatinib and pyrotinib on HUVEC motility and invasive capacity.Results: We showed that HUVEC proliferation, motility and invasive capacity were further enhanced upon incuba

Published
2019

10. [Experimental study on co-culture of early embryo and cancer cells in vitro].

Author

Zhang N, Yan X, Jiang S, Wang G, Lu Z, Ren Y, Mao Y, Kan B, and Luo F

Subjects
Animals, Blastocyst physiology, Cell Line, Tumor, Coculture Techniques, Humans, Male, Melanoma pathology, Mice, Blastocyst cytology, Embryo Culture Techniques methods, Embryo, Mammalian cytology, Liver Neoplasms pathology
Abstract

The co-culture system of early embryos and cancer cells is an important means to observe the biological behavior changes of embryos and cancer cells in vitro. In this study, we co-cultured the 3.5 dpc mouse embryo with malignant tumor cells, investigated the development of blastocyst by observing the hatchment, attachment and outgrowth, observed the biological behavior changes of cancer cells in the embryonic circumstances, and detected the proliferation and apoptosis of cancer cells. Compared with the control, the embryos developed normally in the tumor environments, and the rate of hatchment, attachment and outgrowth increased significantly (P<0.05). However, there was no significant change of cancer cells in morphology, proliferation and apoptosis in the co-culture system (P>0.05). Under the co-culture system, the early embryo developed normally, and the cancer cells also grew well. There may be similarities between the embryos and cancer cell's choice for living. Moreover, the growth of embryos could be promoted by cancer cells in the co-culture system. This might be related to the similarities of gene expression, growth factors and signal transduction mechanisms between embryos and cancer cells.

Published
2009

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