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This study was to analyze the influence of psychological monitors (namely peer counselors with Chinese characteristics) and counselors on the affect of suicide-related interviews among high-risk students. 126 college students (M[subscript age] = 19.75 ± 1.05, aged: 18-23, 60% were male) were randomly divided into experimental group (interviewed) and control group (not interviewed). The experimental group filled in the positive and negative affect scale (PANAS) before and after the interview, while the experimental group and the control group filled in the Beck Hopelessness Scale (BHS) and Centre for Epidemiological Studies Depression Scale (CES-D) before the interview and 1 week after the interview. The results showed that negative affect of the experimental group decreased more after the psychological counselor interviewed the experimental group than the psychological monitors. (short-term effect: p = 0.001, Cohen's d = 0.66; long-term depression: p = 0.007, [eta][subscript p][superscript 2] = 0.057) The interviewer's identity had no effect on the subjects' long-term affect changes. (depression: F = 0.000, p = 0.987; hopelessness: F = 2.960, p = 0.09). Both psychological monitors and counselors can carry out suicide-related interview for high-risk students.

Postharvest fungal pathogens benefit from the increased host susceptibility that occurs during fruit ripening. In unripe fruit, pathogens often remain quiescent and unable to cause disease until ripening begins, emerging at this point into destructive necrotrophic lifestyles that quickly result in fruit decay. Here, we demonstrate that one such pathogen, Botrytis cinerea, actively induces ripening processes to facilitate infections and promote disease in tomato (Solanum lycopersicum). Assessments of ripening progression revealed that B. cinerea accelerated external coloration, ethylene production, and softening in unripe fruit, while mRNA sequencing of inoculated unripe fruit confirmed the corresponding upregulation of host genes involved in ripening processes, such as ethylene biosynthesis and cell wall degradation. Furthermore, an enzyme-linked immunosorbent assay (ELISA)-based glycomics technique used to assess fruit cell wall polysaccharides revealed remarkable similarities in the cell wall polysaccharide changes caused by both infections of unripe fruit and ripening of healthy fruit, particularly in the increased accessibility of pectic polysaccharides. Virulence and additional ripening assessment experiments with B. cinerea knockout mutants showed that induction of ripening depends on the ability to infect the host and break down pectin. The B. cinerea double knockout Δbc polygalacturonase1 Δbc polygalacturonase2 lacking two critical pectin degrading enzymes was incapable of emerging from quiescence even long after the fruit had ripened at its own pace, suggesting that the failure to accelerate ripening severely inhibits fungal survival on unripe fruit. These findings demonstrate that active induction of ripening in unripe tomato fruit is an important infection strategy for B. cinerea.

The automated choreography of dance movements is a new field combining artificial intelligence and dance performance, which has important research value. In this paper, a Transformer-based cheerleading automatic choreography and real-time adjustment algorithm are proposed, which generates cheerleading movements consistent with the music rhythm by stacking multi-layer bidirectional cross-attention layers and introduces an algorithm for real-time adjustment according to the music phrases and the emotions of the dance phrases. The experimental results show that the new algorithm has a dance matching accuracy score of 4.33, which is 0.95 points higher than the accuracy score of manual matching, and 80.76% of the judges think that the overall effect of the cheerleading exercise generated by this algorithm is better than that of the choreography results of the comparison algorithm. This paper’s algorithm has good results, as evidenced by the results.

Objectives Rhabdomyolysis is a series of symptoms caused by the dissolution of striped muscle, and acute kidney injury (AKI) is a potential complication of severe rhabdomyolysis. The underlying causes of AKI are remarkably complex and diverse. Here, we aim to investigate whether pifithrin-α protected against rhabdomyolysis-induced AKI and to determine the involved mechanisms.Methods Intramuscular injection in the right thigh caudal muscle of C57BL/6J mice with 7.5 ml/kg saline (Group A) or of the same volume 50% glycerol was used to induce rhabdomyolysis and subsequent AKI (Group B). Pifithrin-α was injected intraperitoneally 4 h before (Group C) or 4 h after (Group D) the glycerol injection. Serum creatine kinase, blood urea nitrogen, and creatinine were determined, and the renal cortex was histologically analyzed. Renal expression levels of interested mRNAs and proteins were determined and compared, too.Results Intramuscular injection of glycerol induced rhabdomyolysis and subsequent AKI in mice (Groups B–D). Renal function reduction and histologic injury of renal tubular epithelial cells were associated with increased p53 activation, oxidative stress, and inflammation. Notably, compared with pifithrin-α rescue therapy (Group D), pretreatment of pifithrin-α (Group C) protected the mice from severe injury more effectively.Conclusions Our present study suggests that p53 may be a therapeutic target of AKI caused by glycerol, and the inhibition of p53 can block glycerol-mediated AKI by using pharmacological agents instead of genetic inhibitory approaches, which further supports that p53 played a pivotal role in renal tubular injury when challenged with glycerol.