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Bio-based PTT is a green degradable polyester material which can be prepared by the polymerization with bio-based 1,3-propylene glycol (PDO) and terephthalic acid (PTA). In the present work, a bio-based polytrimethylene terephthalate (PTT) antibacterial fabric was fabricated by a traditional dyeing method using a natural acid mordant dye and metal ion. The color of madder dye is sensitive to the outside environment. Different metal ions have great influence on the color of madder dye, which can not only enrich the chromatogram of plant dyes but also improve the dyeing fastness. Furthermore, Bio-based PTT fabric can be dyed with the madder dye under atmospheric dyeing condition by one-bath and optimal Dyeing Conditions were 90–100 °C of dyeing temperature, 40 min of dyeing time and 5–6 pH of dye solution. The antimicrobial test results showed that the dyed PTT fabrics dyed with only madder dye exhibited good antimicrobial properties. In the dyeing process, the introduction of metal silver ion to dyed PTT fabrics enhanced antimicrobial properties and washing fastness. Madder-Ag+ dyed PTT fabric in one bath medium displayed excellent inhibitory effect on the growth and reproduction of S. aureus and E. coli. After 10 times of washing, it still possessed excellent antibacterial performance, and the antibacterial rate still reached 99 %. In our work, the traditional one-bath dyeing method with natural acid mordant dyes and Ag ions can realize coloring and antibacterial properties. The functional application of bio-based PTT materials will be expanded. [ABSTRACT FROM AUTHOR]