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The aim of the study was to investigate the appropriate postharvest storage temperature for Pouteria caimito Radlk., to prolong its storage life, and more broadly to expand the market for Pouteria caimito. The effects of different storage temperatures on the postharvest physiology and storage quality of P. caimito were studied by determining the main quality indices and physiological indices of fruit samples stored at 5, 10, 15 or 20 ℃ after harvest. The results showed that cold storage could maintain the quality of P. caimito to a certain extent, but chilling injury (CI) symptoms, characterized by pitting and water-soaked appearance of the fruit surface and peel browning, appeared after three days of storage at 5 ℃. Compared with the other three storage temperatures, storage at 10 ℃ could maintain higher levels of fruit hardness, total soluble solids (TSS), vitamin C (VC) and total sugar, and lower respiratory intensity and cell membrane permeability. Compared with 20 ℃, storage at 10 ℃ could inhibit the increase of malondialdehyde (MDA) content, peroxidase (POD) and polyphenol oxidase (PPO) activity, thereby delaying the senescence and browning of P. caimito. Therefore, 10 ℃ was the appropriate storage temperature for P. caimito. The results of this study will provide a theoretical basis for postharvest quality maintenance of P. caimito.

Mental health problems leads to serious disease burden among people living with HIV/AIDS (PLHIV). The study aimed at measuring the mental disorders-caused burden of disease based on PLHIV in mainland China. The data used was from the national HIV/AIDS case reporting system, life expectancy (LE) and LE-eliminated suicide were evaluated by the life-table method. The total YLLs and YLLs caused by suicide in each age group were calculated. The disability weights were estimated by the scale of depression symptoms (CES-D) from the multi-center cross-sectional survey, then calculated the corresponding YLDs as a burden of mental illness among PLHIV. Results showed that the LE had been prolonged by implementing antiviral therapy for PLHIV. The proportion of YLLs caused by suicide was the highest (5·46%) in the 15–24 age group. The YLDs in the 25–34 age group were the highest. The YLLs caused by suicide in males were higher than those in the same age group of females. The YLDs and YLLs were higher in heterosexual-infected PLHIV than in homosexual-infected PLHIV, except for YLLs in the 25–34 age group. In summary, this study first provided localized data on the disease burden caused by mental health problems among PLHIV. [ABSTRACT FROM AUTHOR]

Maize productivity in the central belt plays a significant role in the food security of China. With good adaptability and disease resistance, landrace germplasm is important for maize improvement. A total of 246 landrace accessions were collected from the maize belts in central China and genotyped with the SLAF-seq (Specific-Locus Amplified Fragment Sequencing) method, and 144,650 SNPs were obtained for each accession. The results showed that the landrace accessions could be divided into three major groups. In the cluster results, Group I included 64 accessions, which mainly belonged to the landrace of White horse teeth; Group II had 71 accessions, which mainly belonged to the lantern red landraces; the rest of the 116 accessions were clustered as Group III, including a variety of types landraces and seven indicator inbred lines. In the results of structure and multidimensional scaling, the accessions' attribution differed with the clusters, the main reason for which is the attribution change in intermediate germplasms. Linkage disequilibrium decay distance was 0.98 kb, which was much lower than that of temperate and tropical maize inbred lines, indicated the much higher genetic diversity of landrace germplasms. The results can help us select suitable landrace germplasms and speed up the process of inbred line development and maize improvement. [ABSTRACT FROM AUTHOR]

Light-sheet fluorescence microscopy (LSFM) has been widely used to image the three-dimensional (3D) structures and functions of various millimeter-size bio-specimen such as zebrafish. However, the sample adsorption and scattering cause shading of the light-sheet illumination, preventing the even 3D image of thick samples. Herein, we report a continuous-rotational light-sheet microscope (CR-LSM) that enables simultaneous 3D bright-field and fluorescence imaging. With a high-accuracy rotational stage, CR-LSM records the outline projections and the fluorescent images of the sample at multiple rotation angles. Then, 3D morphology and fluorescent structure were reconstructed with a developed algorithm. Using CR-LSM, zebrafish's whole-fish contour and blood vessel structures were obtained simultaneously. [ABSTRACT FROM AUTHOR]