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This paper illustrates the extent of how education background of parents could affect the relationship between children and parents. The inculcate in children is an essential factor that influences the relationship between children and parents. A strict education method may cause a harsh relationship between a child and his parents, while encouraging education is often beneficial for improving parent-child relationships. Children ‘ s education is decided by the education background of parents, as parents with different education backgrounds would educate child differently. Typically, parents who are well-educated perform better in educating children through a reasonable way. The data in this essay were collected through promoting questionnaires on social media, which uses parent-child sub-scale as the basic questionnaire. What’s more, other evidence from previous researches were also used as a reference to guarantee authenticity. This study assumes that high educated parents can build a better parent-child relationship. Correlation analysis shows that significant positive correlation exists between fathers education background and positive father-child relationship, which is also the same for mothers education background and positive mother-child relationship. Furthermore, non-parametric tests also justifies that the education background of a mother shows a significant positive father-child relationship. These all demonstrates the fact that the more educated the parents are, the better parent-child relationship will be.

• DENV2 NS3 helicase activity was stimulated by the N-terminal residues 51 to 83 of NS4B. • Motif I and motif III of NS3 helicase were required for NS3-NS4B interaction. • NS4B coordinated the ATP binding and single-strand DNA dissociation in the unwinding process. Dengue virus NS3 is a prototypical DEx(H/D) helicase that binds and hydrolyzes NTP to translocate along and unwind double-stranded nucleic acids. NS3 and NS4B are essential components of the flavivirus replication complex. Evidences showed that NS4B interacted with NS3 and modulated the helicase activity of NS3. Despite important insights into structural, mechanistic, and cellular aspects of the NS3 function, there is still a gap in understanding how it coordinates the helicase activities within the replicase complex for efficient replication. Here, using the DENV2 as a model, we redefined the critical region of NS4B required for NS3 function by pull-down and MST assays. The FRET-based unwinding assay showed that NS3 would accelerate unwinding duplex nucleic acids in the presence of NS4B (51-83). The simulated NS3-NS4B complex models based on the rigid-body docking delineated the potential interaction sites located in the conserved motif within the core domain of NS3. Mutations in motif I (I190A) and motif III (P319L) of NS3 interfered with the unwinding activity stimulated by NS4B. Upon binding to the NS3 helicase, NS4B assisted NS3 to dissociate from single-stranded nucleic acid and enabled NS3 helicase to keep high activity at high ATP concentrations. These results suggest that NS4B probably serves as an essential cofactor for NS3 to coordinate the ATP cycles and nucleic acid binding during viral genome replication. [ABSTRACT FROM AUTHOR]