14 results on '"Zhai LX"'
Search Results
2. Abnormal performance of peroral endoscopic myotomy (POEM): a case misdiagnosed as achalasia of cardia.
- Author
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Yu WQ, Gao HJ, Zhai LX, and Wei YC
- Subjects
- Male, Humans, Middle Aged, Cardia surgery, Esophagogastric Junction surgery, Diagnostic Errors, Esophageal Achalasia diagnosis, Esophageal Achalasia surgery, Myotomy
- Abstract
Background: Pseudoachalasia is a rare disease that behaves similarly to achalasia (AC), making it sometimes difficult to differentiate., Case Presentation: We report a case of 49-year-old male with adenocarcinoma of the gastroesophageal junction misdiagnosed as achalasia. No obvious abnormalities were found in his initial examinations including upper digestive endoscopy, upper gastrointestinal imaging and chest computed tomography (CT). During the subsequent introduced-peroral endoscopic myotomy (POEM), it was found that the mucosal layer and the muscular layer had severe adhesion, which did not receive much attention, delayed the clear diagnosis and effect treatment, and ultimately led to a poor prognosis for the patient., Conclusions: This case suggests that when patients with AC found mucosal and muscular adhesions during POEM surgery, the possibility should be considered that the lesion may be caused by a malignant lesion., (© 2024. The Author(s).)
- Published
- 2024
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3. Short-term outcome of totally minimally invasive versus hybrid minimally invasive Ivor-Lewis esophagectomy.
- Author
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Yu WQ, Zhai LX, Shi GD, Tang JY, Gao HJ, and Wei YC
- Subjects
- Humans, Retrospective Studies, Esophagectomy adverse effects, Minimally Invasive Surgical Procedures methods, Postoperative Complications epidemiology, Treatment Outcome, Esophageal Neoplasms surgery, Laparoscopy methods
- Abstract
Objectives: For resectable esophageal cancer, the choice of total minimally invasive esophagectomy (TMIE) or hybrid minimally invasive esophagectomy (HMIE) remains controversial. The purpose of this study was to evaluate the short-term clinical outcomes of TMIE and HMIE under the Ivor-Lewis procedure., Methods: The data of 145 patients diagnosed with middle or lower esophageal cancer who underwent radical Ivor-Lewis esophagectomy in the Affiliated Hospital of Qingdao University between January 2018 and December 2019 were retrospectively analyzed. The short-term outcomes such as complications during surgery or within 30 days after surgery and postoperative pain were analyzed., Results: All patients were divided into TMIE group (75 patients) and HMIE group (70 patients). No significant difference was observed in the baseline characteristics of the two groups. TMIE was associated with less blood loss than the HMIE group (p < 0.05). A total of 54 (37.2%) patients had postoperative complications. Although the two groups were statistically similar in the incidence of major complications, patients in the HMIE group were more likely to have pneumonia compared with those in the TMIE group. The numeric rating scale for pain was significantly higher in the HMIE group (p = 0.002) and more patients required an additional opioid analgesia after esophagectomy (p = 0.282)., Conclusions: In conclusion, according to perioperative outcomes, TMIE can benefit patients better than HMIE., Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest, and there is no funding for the work., (Copyright © 2023 Asian Surgical Association and Taiwan Robotic Surgery Association. Published by Elsevier B.V. All rights reserved.)
- Published
- 2023
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4. P2Y1R Ligation Suppresses Th17 Cell Differentiation and Alleviates Colonic Inflammation in an AMPK-Dependent Manner.
- Author
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Chang YY, Huan QC, Peng J, Bi WC, Zhai LX, Chen Y, Lamb JR, Shen XC, Bian ZX, Wu HQ, Cheng YX, and Xiao HT
- Subjects
- AMP-Activated Protein Kinases metabolism, Animals, Cell Differentiation, Inflammation metabolism, Interleukin-17 metabolism, Mice, Th17 Cells, Colitis chemically induced, Colitis metabolism, Nuclear Receptor Subfamily 1, Group F, Member 3 genetics, Nuclear Receptor Subfamily 1, Group F, Member 3 metabolism
- Abstract
P2Y1 receptor is a G-protein-coupled receptor that plays a critical role in the immune response of inflammatory bowel diseases. However, its regulatory effects on CD4
+ T cell response have not been fully elucidated. The study aimed to characterize the role of P2Y1R in Th17 cell differentiation and colonic inflammation. Our results demonstrated that P2Y1R was significantly increased in the splenocytes of colitic mice, which was positively associated with the expression of RORγt and IL-17A. P2Y1R deficiency significantly ameliorated DSS-induced colitis and its Th17 responses. In parallel, P2Y1R deficiency greatly impaired the differentiation of Th17 cell, down-regulated the mRNA expression of IL-17A and RORγt, and protein expression of RORγt in vitro . More importantly, it was found that P2Y1R deficiency markedly increased AMPK phosphorylation of Th17 polarized CD4+ T cells, and antagonist of AMPK significantly reversed the inhibitory effect of P2Y1R deficiency on Th17 cell generation in vivo and in vitro . Overall, these findings demonstrated that P2Y1R deficiency could suppress Th17 cell differentiation in an AMPK-dependent manner to ameliorate colitis, and P2Y1R can act as an important regulator of Th17 cell differentiation to control colonic inflammation., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Chang, Huan, Peng, Bi, Zhai, Chen, Lamb, Shen, Bian, Wu, Cheng and Xiao.)- Published
- 2022
- Full Text
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5. [Preparation and Application of Magnetic Water Treatment Materials Based on Iron Sludge].
- Author
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Zeng HP, Zhai LX, Li D, and Zhang J
- Subjects
- Iron, Magnetic Phenomena, Sewage, Water Pollutants, Chemical analysis, Water Purification
- Abstract
In recent years, solid waste iron sludge (red mud, iron-containing water treatment residues, and iron-rich sludge) has been widely used to remove pollutants in the water environment; however, the difficulty of separating powdered iron sludge from the water environment media makes it impossible to apply it as a water treatment material on a large scale, and preparing iron sludge into magnetic materials that are easy to be separated is one of the effective strategies to solve this bottleneck. According to the existing research on iron sludge-based magnetic materials at home and abroad, the preparation methods of magnetic materials using iron sludge as raw materials are summarized, including a series of methods, including the thermal decomposition method, hydrothermal and solvothermal method, co-precipitation method, reduction roasting method, and carbonization method. Additionally, it is pointed out that the currently commonly used preparation methods are thermal decomposition, hydrothermal and solvothermal, and co-precipitation. In addition, the performance and application of these magnetic materials as adsorbents or catalysts in water treatment are also summarized. In general, iron sludge-based magnetic materials can better absorb heavy metals and organic pollutants in water. The main adsorption mechanisms are complexation, electrostatic interaction, reduction, cation exchange, and precipitation. As a catalyst, it can efficiently oxidize and degrade organic pollutants by generating strong oxidizing substances:SO
4 - · and ·OH. Although there have been many studies on the preparation and application of iron sludge-based magnetic materials, because the raw material iron sludge contains many impurities, the magnetic materials prepared from iron sludge also have certain impurities. Therefore, it is still necessary to strengthen the research on the safety of iron sludge-based magnetic materials in the future to further ensure that they can be used as environmentally friendly materials for water environment restoration.- Published
- 2022
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6. Author Correction: Cyclocarya paliurus tea leaves enhances pancreatic β cell preservation through inhibition of apoptosis.
- Author
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Xiao HT, Wen B, Ning ZW, Zhai LX, Liao CH, Lin CY, Mu HX, and Bian ZX
- Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
- Published
- 2020
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7. Anti-inflammatory properties of uvaol on DSS-induced colitis and LPS-stimulated macrophages.
- Author
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Du SY, Huang HF, Li XQ, Zhai LX, Zhu QC, Zheng K, Song X, Xu CS, Li CY, Li Y, He ZD, and Xiao HT
- Abstract
Background: Apocynum venetum leaves are used as a kind of phytomedicine and the main ingredient in some traditional Chinese medicine products for the relief of colitis. To understand the bioactive constituents of A. venetum L., we did a phytochemistry study and investigated anti-Inflammatory effects of compounds and explored the underlying mechanisms., Methods: We isolated compounds from ethanol extract of A. venetum L. leaf and detected the most effective compound by NO inhibition assay. We investigated anti-Inflammatory effects on dextran sulfate sodium (DSS)-induced colitis mice and lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The disease activity index was determined by scores of body weight loss, diarrhea and rectal bleeding; histological damage was analyzed by H&E staining; macrophages change in the colon were analyzed by immunohistochemistry (IHC); myeloperoxidase activity was measured by myeloperoxidase assay kits; levels of proinflammatory cytokines were determined by qPCR and ELISA; protein production such as COX-2, iNOS, STAT3 and ERK1/2 were determined by western blotting., Results: We isolated uvaol from ethanol extract of A. venetum L. leaf and found uvaol has excellent potential of inhibiting NO production. We further found uvaol could attenuate disease activity index (DAI), colon shortening, colon injury, and colonic myeloperoxidase activity in DSS-induced colitis mice. Moreover, uvaol significantly reduces mRNA expression and production of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β, and MCP-1) and infiltration of macrophages in colonic tissues of colitis mice. Studies on LPS challenged murine macrophage RAW246.7 cells also revealed that uvaol reduces mRNA expression and production of pro-inflammatory cytokines and mediators. Mechanically, uvaol inhibits the pro-inflammatory ERK/STAT3 axis in both inflamed colonic tissues and macrophages., Conclusions: A. venetum leaf contains uvaol and uvaol has potent anti-inflammatory effects on DSS-induced experimental colitis and LPS-stimulated RAW264.7 macrophage cells. These results suggest uvaol is a prospective anti-inflammatory agent for colonic inflammation., Competing Interests: Competing interestsThe authors declare that they have no competing interests., (© The Author(s) 2020.)
- Published
- 2020
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8. Simultaneous UPLC-TQ-MS/MS determination of six active components in rat plasma: application in the pharmacokinetic study of Cyclocarya paliurus leaves.
- Author
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Ning ZW, Zhai LX, Peng J, Zhao L, Huang T, Lin CY, Chen WH, Luo Z, Xiao HT, and Bian ZX
- Abstract
Background: Cyclocarya paliurus (Batal.) Ijinskaja (CP) is a monotypic genus plant, also called sweet tea tree that belongs to the Juglandaceae family, which is mainly distributed in the subtropical highlands in China. Our previous work has verified that CP leaves exhibit a potent hyperglycemic effect by inhibiting pancreatic β cell apoptosis through the regulation of MPAK and Akt signaling pathways. However, the components that contribute to this potential health benefit remain undiscovered., Method: A sensitive, reliable, and validated ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UPLC-TQ-MS/MS) method was developed to simultaneously determine the presence of six active components (neochlorogenic acid, chlorogenic acid, quercetin-3- O -glucuronide, kaempferol-3- O -rhamnoside, quercetin, and kaempferol) in rat plasma after a single oral administration (in a dosage of 10.5 g/kg) of an extract of CP leaves to rats. The separation was performed on a Waters ACQUITY BEH C
18 column (50 mm × 2.1 mm, 1.7 μm). The detection was conducted by multiple reaction monitoring (MRM) in negative ionization mode. The two highest abundant MRM transitions without interference were optimized for each analyte. Acetonitrile and formic acid aqueous solution (0.1%) was used as the mobile phase at a flow rate of 0.3 ml/min., Result: The precision, accuracy, and recovery all satisfied the criteria of international guidance (Bioanalytical Method Validation Guidance for Industry, Food and Drug Administration), and the analytes were stable in plasma for all tested conditions. The main pharmacokinetic parameters were calculated by plasma concentration versus time profiles using the pharmacokinetics program., Conclusion: The pharmacokinetic parameters of each compound can facilitate future clinical studies., Competing Interests: Competing interestsThe authors declare that they have no competing interests.- Published
- 2019
- Full Text
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9. Identification of α-glucosidase inhibitors from cyclocarya paliurus tea leaves using UF-UPLC-Q/TOF-MS/MS and molecular docking.
- Author
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Ning ZW, Zhai LX, Huang T, Peng J, Hu D, Xiao HT, Wen B, Lin CY, Zhao L, and Bian ZX
- Subjects
- Animals, Chromatography, High Pressure Liquid, Glycoside Hydrolase Inhibitors administration & dosage, Humans, Hyperglycemia drug therapy, Hypoglycemic Agents administration & dosage, Hypoglycemic Agents chemistry, Male, Mice, Mice, Inbred C57BL, Molecular Docking Simulation, Plant Extracts administration & dosage, Plant Leaves chemistry, Tandem Mass Spectrometry, alpha-Glucosidases metabolism, Glycoside Hydrolase Inhibitors chemistry, Hyperglycemia enzymology, Juglandaceae chemistry, Plant Extracts chemistry, alpha-Glucosidases chemistry
- Abstract
Leaves of Cyclocarya paliurus (CP) have a potential antihyperglycemic effect, but its active compositions responsible for the beneficial properties remain unclear. The CP extract exhibited remarkable α-glucosidase inhibitory activity with an IC50 value of 31.5 ± 1.05 μg mL-1, much lower than that of the positive control acarbose (IC50 = 296.6 ± 1.06 μg mL-1). To identify the specific α-glucosidase inhibitors from the CP extract, affinity ultrafiltration coupled with ultra-performance liquid chromatography and quadrupole-time-of-flight mass spectrometry (UF-UPLC-Q/TOF-MS/MS) was developed and 11 potential α-glucosidase inhibitors from CP extract were identified. In vitro α-glucosidase inhibitory assay verified that quercetin-3-O-glucuronide, kaempferol-3-O-rhamnoside, quercetin, kaempferol, asiatic acid and genistein were primarily responsible for the α-glucosidase inhibitory activity of the CP extract. Further, a hypoglycemia test also verified that these α-glucosidase inhibitors had the potential to reduce post-prandial hyperglycaemia in C57BL/6 mice. Moreover, the molecular docking study revealed that these identified α-glucosidase inhibitors more easily occupy the active sites of α-glucosidase than does the positive control acarbose. These findings suggest the CP tea leaves are the potential source of a hypoglycaemic agent.
- Published
- 2019
- Full Text
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10. Cyclocarya paliurus tea leaves enhances pancreatic β cell preservation through inhibition of apoptosis.
- Author
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Xiao HT, Wen B, Ning ZW, Zhai LX, Liao CH, Lin CY, Mu HX, and Bian ZX
- Subjects
- Animals, Apoptosis drug effects, Blood Glucose drug effects, Cardiomegaly metabolism, Diabetes Mellitus, Experimental blood, Diabetes Mellitus, Experimental complications, Diabetes Mellitus, Experimental metabolism, Diabetic Nephropathies metabolism, Diet, High-Fat adverse effects, Eating drug effects, Fatty Liver metabolism, Gene Expression Regulation drug effects, Insulin blood, Insulin-Secreting Cells drug effects, Mice, Plant Extracts pharmacology, Plant Leaves chemistry, Signal Transduction drug effects, Streptozocin, Cardiomegaly drug therapy, Diabetes Mellitus, Experimental drug therapy, Diabetic Nephropathies drug therapy, Fatty Liver drug therapy, Insulin-Secreting Cells cytology, Juglandaceae chemistry, Plant Extracts administration & dosage
- Abstract
Leaves of Cyclocarya paliurus are a sweet tea traditionally used to treat obesity and diabetes in China. However, its protective mechanisms against hyperglycemia remains unclear. Here, we demonstrate that the extract of C. paliurus leaves significantly decreased body loss, food intake and blood glucose level, and increased blood insulin level, β-cell number and insulin-producing β cells in high-fat diet-low dose STZ-induced diabetic mice. In vivo and in vitro studies also showed the extract of C. paliurus leaves significantly inhibited pancreatic β cell apoptosis by suppressing the expression of caspase 8, caspase 9 and cleaved caspase-3, as well as Bax/Bcl-2 ratio, down-regulating p38, ERK and JNK phosphorylation, and up-regulating Akt phosphorylation. These effects were significantly enhanced by inhibitor p-38 or ERK or JNK, and counteracted by inhibitor of PI3K. In addition, the extract of C. paliurus leaves also significantly improved hepatic steatosis, nephropathy and cardiac hypertrophy of diabetic mice. Taken together, these results provide the insight into the effects of C. paliurus leaves on pancreatic β cell preservation in standing glucolipotoxicity. Therefore, C. paliurus tea leaves may be used as a new remedy for diabetes through enhancing pancreatic β cell preservation by inhibiting β cell apoptosis.
- Published
- 2017
- Full Text
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11. First Report of Peach Brown Rot Caused by Monilinia fructicola in Central and Western China.
- Author
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Yin LF, Chen SN, Yuan NN, Zhai LX, Li GQ, and Luo CX
- Abstract
Brown rot of peach (Prunus persica) in China has been reported to be caused by at least three Monilinia species (1). In the present study, peaches with symptoms resembling brown rot caused by Monilinia species were collected from commercial orchards in the northwestern province of Gansu in August 2010, the southwestern province of Yunnan in July 2011, and in the central province of Hubei in July 2012. Affected fruit showed the typical symptoms of brown rot with zones of sporulation. Fungal isolates were single-spored and cultured on potato dextrose agar (PDA). Colonies showed grayness with concentric rings of sporulation after incubation at 25°C in the dark. Mean mycelial growth of isolates YHC11-1a and YHC11-2a from Yunnan, GTC10-1a and GTC10-2a from Gansu, and HWC12-14a and HWC12-23a from Hubei, was 4.6 ± 0.4 and 7.5 ± 0.7 cm after 3 and 5 days incubation, respectively. Conidia were lemon shaped and formed in branched monilioid chains, and the mean size was 9.3 (6.7 to 11.5) × 12.5 (7.9 to 17.8) μm, which was consistent with the characteristics of Monilinia fructicola (1,2). The species identification was confirmed by sequencing of the ribosomal ITS sequences. The ribosomal ITS1-5.8S-ITS2 region was amplified from each of the six isolates using primers ITS1 and ITS4 (3). Results indicated that the ITS sequences of these isolates were identical and showed the highest similarity (100%) with M. fructicola ITS sequences from isolates collected in China (GenBank Accession Nos. HQ893748, FJ515894, and AM887528), Slovenia (GU967379), Italy (FJ411109), and Spain (EF207423). The pathogen was also confirmed to be M. fructicola based on the detection of an M. fructicola- specific band (534 bp) using a PCR-based molecular tool developed for distinguishing Chinese Monilinia species affecting peach (1). Pathogenicity was tested on surface-sterilized, mature peaches (Shui Mi Tao) with representative isolates. Fruits were holed at three equidistant positions to a depth of 5 mm using a sterile cork borer. Mycelial plugs (5 mm in diameter) from the periphery of a 4-day-old colony of each isolate were placed upside down into each hole, control fruits received water agar. After 3 days of incubation at 22°C in a moist chamber, inoculated fruits developed typical brown rot symptoms while control fruits remained healthy. Pathogens from the inoculated fruit were confirmed to be M. fructicola based on morphological characteristics. To our knowledge, this is the first report of occurrence of M. fructicola in Gansu, Yunnan, and Hubei provinces, thousands of kilometers away from eastern China where occurrence of peach brown rot caused by M. fructicola has been confirmed (2,4). The results indicated the further geographical spread of the M. fructicola in China. References: (1) M. J. Hu et al. Plos One 6(9):e24990, 2011. (2) M. J. Hu et al. Plant Dis. 95:225, 2011. (3) T. J. White et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Academic Press, San Diego, 1990. (4) X. Q. Zhu et al. Plant Pathol. 54:575, 2005.
- Published
- 2013
- Full Text
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12. Poly[diaqua-μ(4)-oxalato-di-μ(6)-phosphato-tetra-cobalt(II)].
- Author
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Wu WY and Zhai LX
- Abstract
In the structure of the title compound, [Co(4)(C(2)O(4))(PO(4))(2)(H(2)O)(2)](n), there are layers composed of the phosphate anions and two independent Co(II) cations. These layers are parallel to (001) and are bridged by the oxalate anions that are situated in special positions on centres of symmetry. One independent Co atom has an octa-hedral coordination, while the second independent Co atom is coordinated in a trigonal-bipyramidal coordination that includes the water mol-ecule. The crystal packing is stabilized by O-H⋯O hydrogen bonds between the coordinated water mol-ecules and oxalate O atoms.
- Published
- 2008
- Full Text
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13. [The empirical study of Maxingganshi decoction and its compatibility in acute lung injury rats].
- Author
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Wang WG, Shi XG, Zhai LX, Wang PJ, Peng XF, and Xu YY
- Subjects
- Acute Disease, Animals, Capillary Permeability drug effects, Disease Models, Animal, Drug Combinations, Drugs, Chinese Herbal chemistry, Drugs, Chinese Herbal therapeutic use, Female, Interleukin-10 blood, Lung Diseases blood, Lung Diseases pathology, Male, Pulmonary Edema blood, Pulmonary Edema pathology, Rats, Rats, Sprague-Dawley, Systemic Inflammatory Response Syndrome blood, Systemic Inflammatory Response Syndrome drug therapy, Systemic Inflammatory Response Syndrome pathology, Tumor Necrosis Factor-alpha blood, Drugs, Chinese Herbal pharmacology, Lung Diseases drug therapy, Plants, Medicinal chemistry, Pulmonary Edema drug therapy
- Abstract
Objective: To explore the pharmacodyniamic action and the mechanism of action of the Maxingganshi decoction in acute lung injury rats in order to supply the pharmacology evidence to cure the SIRS-ALI of Maxingganshi decoction., Methods: The study designed by orthogonal design. The SIRS-ALI model were Conseructed by the LPS intravenous injection and the effects of Maxingganshi decoction and it's different compatibilities to the SIRS-ALI model were observed. The experiments results were analvsised in order to reveal the compatibility rules of the Maxingganshi decocotion., Results: Maxingganshi decocotion and its different compatibilities had good effects of prevention and cure the SIRS-ALI. The mechanism maybe concerned with the Maxingganshi decocotion can decrease the TNF-alpha and increase the IL-10. The experiments results also indicated that the action of the full formula was the best. The principal drug was the most important in the formula., Conclusion: Maxingganshitang has a good effect in anti the ALI; the results indicat that the principal drug is the predominant therapeutic action in the formula ministerial drug. Adjuvant drug and messenger drug can strengthen pharmacodynamic action.
- Published
- 2007
14. [Influence of compatibility on content of berberine in Maxingganshi decoction].
- Author
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Wang WG, Shi XG, Zhai LX, Wang PJ, Peng XF, and Xu YY
- Subjects
- Amygdalin analysis, Amygdalin isolation & purification, Berberine isolation & purification, Drug Combinations, Drugs, Chinese Herbal isolation & purification, Ephedrine analysis, Ephedrine isolation & purification, Glycyrrhiza uralensis chemistry, Glycyrrhizic Acid analysis, Glycyrrhizic Acid isolation & purification, Prunus chemistry, Reproducibility of Results, Technology, Pharmaceutical methods, Berberine analysis, Chromatography, High Pressure Liquid methods, Drugs, Chinese Herbal analysis, Plants, Medicinal chemistry
- Abstract
Objective: To set up the quantitative method of herberine in decoctions prepared from various combinations of Maxingganshi decotion by HPLC and to determine the change of general contents in different decoction., Methods: The study was designed by L8 (2(7)) orthogonal and to determine the change of general contents in different decoction by HPLC., Results: In full formula remove Glycyrrhiza uralensis group, the contents of L-ephedrine, d-pseudephedrine and the amygdaloside cut down significantly. In full formula remove armeniacae semen group, the content of glycyrrhetic acid cut down significantly. Four chemical composition in full formula group had higher contents., Conclusion: Armeniacae semen can decrease the content of L-ephedrine, D-pseudephedrine. Glycyrrhiza uralensis can help decoctum amygdaloside and armeniacae semen can help decoctum glycyrrhizic acid.
- Published
- 2007
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