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27 results on '"Zhai, Yuanxin"'

6. Specific anti‐glioma targeted‐delivery strategy of engineered small extracellular vesicles dual‐functionalised by Angiopep‐2 and TAT peptides

Author

Zhu, Zhanchi, primary, Zhai, Yuanxin, additional, Hao, Ying, additional, Wang, Quanwei, additional, Han, Fang, additional, Zheng, Wenlong, additional, Hong, Jing, additional, Cui, Leisha, additional, Jin, Wei, additional, Ma, Sancheng, additional, Yang, Lingyan, additional, and Cheng, Guosheng, additional

Published
2022
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14. PAX7 promotes CD49f‐positive dairy goat spermatogonial stem cells' self‐renewal

Author

Du, Xiaomin, primary, Wu, Siyu, additional, Wei, Yudong, additional, Yu, Xiuwei, additional, Ma, Fanglin, additional, Zhai, Yuanxin, additional, Yang, Donghui, additional, Zhang, Mengfei, additional, Liu, Wenqing, additional, Zhu, Haijing, additional, Wu, Jiang, additional, Liao, Mingzhi, additional, Li, Na, additional, Bai, Chunling, additional, Li, Guangpeng, additional, and Hua, Jinlian, additional

Published
2020
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18. PAX7 promotes CD49f‐positive dairy goat spermatogonial stem cells' self‐renewal.

Author

Du, Xiaomin, Wu, Siyu, Wei, Yudong, Yu, Xiuwei, Ma, Fanglin, Zhai, Yuanxin, Yang, Donghui, Zhang, Mengfei, Liu, Wenqing, Zhu, Haijing, Wu, Jiang, Liao, Mingzhi, Li, Na, Bai, Chunling, Li, Guangpeng, and Hua, Jinlian

Subjects
GOATS, STEM cells, GERM cells, GOAT diseases, SPERMATOGENESIS, TRANSCRIPTION factors, CELL lines
Abstract

Spermatogenesis is a complex process that originates from and depends on the spermatogonial stem cells (SSCs). The number of SSCs is rare, which makes the separation and enrichment of SSCs difficult and inefficient. The transcription factor PAX7 maintains fertility in normal spermatogenesis in mice. However, for large animals, much less is known about the SSCs' self‐renewal regulation, especially in dairy goats. We isolated and enriched the CD49f‐positive and negative dairy goat testicular cells by magnetic‐activated cell sorting strategies. The RNA‐ sequencing and experimental data revealed that cells with a high CD49f and PAX7 expression are undifferentiated spermatogonia in goat testis. Our findings indicated that ZBTB16 (PLZF), PAX7, LIN28A, BMPR1B, FGFR1, and FOXO1 were expressed higher in CD49f‐positive cells as compared to negative cells and goat fibroblasts cells. The expression and distribution of PAX7 in dairy goat also have been detected, which gradually decreased in testis tissue along with the increasing age. When the PAX7 gene was overexpressed in dairy goat immortal mGSCs‐I‐SB germ cell lines, the expression of PLZF, GFRα1, ID4, and OCT4 was upregulated. Together, our data demonstrated that there is a subset of spermatogonial stem cells with a high expression of PAX7 among the CD49f+ spermatogonia, and PAX7 can maintain the self‐renewal of CD49f‐positive SSCs. [ABSTRACT FROM AUTHOR]

Published
2021
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19. Amorphous curcumin-based hydrogels to reduce the incidence of post-surgical intrauterine adhesions

Author

Zhang, Wenya, He, Yuxin, Chu, Yun, Zhai, Yuanxin, Qian, Song, Wang, Xinhui, Jiang, Pengju, Cui, Pengfei, Zhang, Yin, and Wang, Jianhao

Abstract

The incidence of intrauterine adhesions (IUA) has increased with the rising utilization of intrauterine surgery. The postoperative physical barrier methods commonly used, such as balloons and other fillers, have limited effectiveness and may even cause further damage to the remaining endometrial tissue. Herein, we developed an injectable thermosensitive hydrogel using Pluronic F127/F68 as pharmaceutical excipients and curcumin as a natural active molecule. The hydrogel effectively addresses solubility and low bioavailability issues associated with curcumin. In vitro, drug release assays revealed that the amorphous curcumin hydrogel promotes dissolution and sustained release of curcumin. In vitroexperiments reveal high biocompatibility of the hydrogel and its ability to enhance vascular formation while inhibiting the expression of fibrotic factor TGF-β1. To assess the effectiveness of preventing IUAs, in vivoexperiments were conducted using IUA rats and compared with a class III medical device, a new-crosslinked hyaluronic acid (NCHA) gel. According to the study, curcumin hydrogel is more effective than the NCHA group in improving the regeneration of the endometrium, increasing the blood supply to the endometrium and reducing the abnormal deposition of fibrin, thus preventing IUA more effectively. This study provides a promising strategy for treating and preventing IUA.

Published
2024
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24. miR‐19b‐3p induces cell proliferation and reduces heterochromatin‐mediated senescence through PLZF in goat male germline stem cells.

Author

Daguia Zambe, John Clotaire, Zhai, Yuanxin, Zhou, Zhe, Du, Xiaomi, Wei, Yudong, Ma, Fanglin, and Hua, Jinlian

Subjects
*ZINC-finger proteins, *SPERMATOGENESIS in animals, *MICRORNA, *CELL proliferation, *HETEROCHROMATIN, *GOAT genetics
Abstract

Promyelocytic leukemia zinc finger PLZF, known as ZBTB16 or ZFP145 is a critical zinc finger protein of male germline stem cells (mGSCs), it's an essential transcriptional factor for goat testis development and spermatogenesis. Loss of PLZF results in progressive depletion of SSCs after the first wave of spermatogenesis leading to eventual spermatogenic arrest, apparently the result of a shift in the balance in SSC fate away from self‐renewal and toward differentiation. Cumulating evidences have demonstrated that microRNAs are expressed in a cell‐specific or stage‐specific manner during spermatogenesis and acts as regulators on specific makers such as Stra8, ETV5, and PLZF. However, the post transcriptional function of PLZF still poorly elucidate in mGSCs. Bioinformatic analysis and dual luciferase reporter assay showed that miR‐19b‐3p binds the 3′UTR of PLZF, suggesting that PLZF is a direct target of miR‐19b‐3p. The profile of miR‐19b‐3p and PLZF analyzed in dairy goat testis at different age showed that miR‐19b‐3p was significantly up‐regulated in goat testis at 1, 3, 6 months and downregulated at 12, 18, and 24 months which was inversely correlated with PLZF in the same testis. Focusing on the role of miR‐19b‐3p, we found that miR‐19b‐3p changes c‐KIT and mTOR signaling through PLZF to promote proliferation in goat nGSCs and infertile mice testes. Over‐expression of PLZF significantly reversed miR‐19b‐3p‐mediated proliferation in mice testes. We found also that miR‐19b‐3p reduced heterochromatin‐mediated senescence through PLZF localized on HP1α. Taken together, our findings indicate that miR‐19b‐3p promotes proliferation and reduces heterochromatin‐mediated senescence through PLZF in mGSCs. [ABSTRACT FROM AUTHOR]

Published
2018
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