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23 results on '"Zhai, Xinyan"'

3. Immune cell early activation, apoptotic kinetic, and T-cell functional impairment in domestic pigs after ASFV CADC_HN09 strain infection

Author

Tian, Yunfei, primary, Wang, Dongyue, additional, He, Shicheng, additional, Cao, Zhen, additional, Li, Wencai, additional, Jiang, Fei, additional, Shi, Yifan, additional, Hao, Yuxin, additional, Wei, Xinhao, additional, Wang, Qingqing, additional, Qie, Shuai, additional, Wang, Jiangtao, additional, Li, Ting, additional, Hao, Xiaoli, additional, Zhu, Jianzhong, additional, Wu, Jiajun, additional, Shang, Shaobin, additional, and Zhai, Xinyan, additional

Published
2024
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9. Pathogenic pseudorabies virus, China, 2012

Author

Yu, Xiuling, Zhou, Zhi, Hu, Dongmei, Zhang, Qian, Han, Tao, Li, Xiaoxia, Gu, Xiaoxue, Yuan, Lin, Zhang, Shuo, Wang, Baoyue, Qu, Ping, Liu, Jinhua, Zhai, Xinyan, and Tian, Kegong

Subjects
Epidemics -- Research -- China, Aujeszky's disease -- Statistics, Swine -- Diseases, Health
Abstract

Pseudorabies virus (PRV), also called Aujeszky disease virus or suid herpesvirus type 1, is a member of the Alphaherpesvirinae subfamily within the family Herpesviridae. This pathogen has major economic consequences [...]

Published
2014
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10. Investigation on pseudorabies prevalence in Chinese swine breeding farms in 2013-2016

Author

Song Xiaohui, Wang Chuanbin, Jianqiang Ni, Liu Yingyi, Bolin Zhao, Yuan Lin, Zhang Shuo, Yang Lin, Wang Jing, Li Shuo, Zhou Zhi, Zhai Xinyan, Xu Qi, and Jiajun Wu

Subjects
0301 basic medicine, Veterinary medicine, China, Farms, Swine, viruses, animal diseases, Pseudorabies, Biology, Breeding, Antibodies, Viral, Vaccines, Attenuated, Virus, 03 medical and health sciences, Food Animals, Pseudorabies Virus PRV, Prevalence, Pseudorabies Vaccines, Animals, Swine Diseases, Attenuated vaccine, virus diseases, Outbreak, biochemical phenomena, metabolism, and nutrition, Serum samples, biology.organism_classification, Herpesvirus 1, Suid, 030104 developmental biology, Animal Science and Zoology
Abstract

Pseudorabies (PR) has been prevalent in Chinese swine breeding farms since the outbreak at the end of 2011. For investigating current prevalence of PR, a nationwide surveillance has been performed in this study. The swine serum samples were collected from 93, 100, 92, and 91 swine farms in China during 2013–2016, respectively. Since the extensive use of gE-deleted pseudorabies virus (PRV) vaccine, we could apply the PRV-gE antibody for determining wild-type virus infection and the PRV-gB antibody for evaluating vaccine immunization. The results were concluded as follows: (1) Nationally, the positive rate of PRV-gB was maintained at a high level (> 90%), while the positive rate of PRV-gE continued to decrease (from 22.17 to 13.14%). (2) The positive rates of PRV-gE were greatly varied in different geographical regions and swine farms (0~100%), while the positive rate of PRV-gB was generally high (> 90%). (3) The number of imported PRV attenuated vaccines were about twice that of domestic PRV attenuated vaccines, while the positive rate of PRV-gB was not significantly different (P > 0.05). (4) The performance of PR eradication developing or developed farms was better than the performance of common farms, with higher positive rate of PRV-gB (> 90%) and much lower positive rate of PRV-gE (nearly 0%).

Published
2017

12. Analysis of Genetic Variation of Two NADC30-like Strains of Porcine Reproductive and Respiratory Syndrome Virus in China

Author

Zhai Xinyan, Bo Hou, Jiajun Wu, Zhou Zhi, Yinyi Liu, Zhang Shuo, Xu Qi, Han Tao, Dong yue Wang, Wang Jing, and Shengpeng Xin

Subjects
0301 basic medicine, Genetics, medicine.medical_specialty, Genetic diversity, Traditional medicine, biology, Phylogenetic tree, Porcine reproductive and respiratory syndrome virus, biology.organism_classification, Homology (biology), Virus, Article, 03 medical and health sciences, 030104 developmental biology, Phylogenetics, NADC30-like strain, Molecular genetics, Genetic variation, medicine, PPRSV
Abstract

Background: NADC30-like strains of porcine reproductive and respiratory syndrome virus first appeared in Chinese swine herds in 2012. Objective and Method: To explore the possible genetic diversity of these strains, we sequenced and analyzed the complete genomes of two NADC30-like strains. These isolates shared 95.4% homology with NADC30. Result: The two strains displayed a discontinuous deletion of 131 amino acids in NSP2, mutations of amino acids in GP3 and GP5, and a 3-nucleotide deletion in the 3′ untranslated region. Phylogenetic analysis showed that the two isolates formed a new branch and clustered in a subgroup with NADC30 isolates from North America. Conclusion: We conclude that the above two NADC30-like strains may have been introduced from North America to China, where they acquired new genetic diversity.

Published
2016

16. Assessment of the Safety and Efficacy of an Attenuated Live Vaccine Based on Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus

Author

Yu, Xiuling, primary, Zhou, Zhi, additional, Cao, Zhen, additional, Wu, Jiajun, additional, Zhang, Zhongqiu, additional, Xu, Baiwan, additional, Wang, Chuanbin, additional, Hu, Dongmei, additional, Deng, Xiaoyu, additional, Han, Wei, additional, Gu, Xiaoxue, additional, Zhang, Shuo, additional, Li, Xiaoxia, additional, Wang, Baoyue, additional, Zhai, Xinyan, additional, and Tian, Kegong, additional

Published
2015
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22. [Expression and identification of truncated Nsp7 protein of North American and Europe genotype porcine reproductive and respiratory syndrome virus].

Author

Qiu P, Ning K, Cai L, Liu Q, Wang B, Zhai X, Yu X, Ni J, and Tian K

Subjects
Animals, Genotype, Porcine respiratory and reproductive syndrome virus classification, Recombinant Proteins biosynthesis, Recombinant Proteins immunology, Swine, Viral Nonstructural Proteins biosynthesis, Porcine respiratory and reproductive syndrome virus genetics, Porcine respiratory and reproductive syndrome virus immunology, Viral Nonstructural Proteins immunology
Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) non-structural protein 7 (Nsp7) plays an important role in the induction of host humoral immune response and could serve as an ideal antigen for serological genotyping assay for PRRSV based on the significant difference in immunoreactivities of North American (NA) and European (EU) PRRSV Nsp7. In this study, Nsp7 of NA and EU PRRSVwas separately expressed and purified using prokaryotic expression system. The purified recombinant Nsp7 proteins reacted with serum antibodies against corresponding genotype PRRSV in Western blotting. However, nonspecific reaction of whole recombinant Nsp7 with antibodies against another genotype PRRSV was observed, indicating that whole NA PRRSV Nsp7 and EU PRRSV Nsp7 have similar antigenic epitopes and recombinant proteins could not be used for genotyping of antibodies against PRRSV. Based on the analysis of similar antigenic epitopes at the hydrophilic region of NA PRRSV Nsp7 and EU PRRSV Nsp7 by bioinformatics assessment, partial Nsp7 gene region deleted sequences encoding similar antigenic epitopes was constructed by fusion PCR. The recombinant truncated Nsp7 (NA-deltaNsp7 and EU-deltaNsp7, about 43 kDa) was expressed and the molecular weight was about 43 kDa. The results of Western blotting showed that NA-deltaNSP7 and EU-deltaNSP7 could be specifically recognized by positive serum to NA or EU PRRSV individually and nonspecific reaction was eliminated. This study provided a basis for further development of serological genotyping assay for North American and European genotype PRRSV infection.

Published
2013

23. Complete genome sequence of two novel Chinese virulent porcine reproductive and respiratory syndrome virus variants.

Author

Zhou Z, Li X, Liu Q, Hu D, Yue X, Ni J, Yu X, Zhai X, Galliher-Beckley A, Chen N, Shi J, and Tian K

Subjects
Animals, Base Sequence, China, Molecular Sequence Data, Porcine Reproductive and Respiratory Syndrome pathology, Sequence Alignment, Sequence Deletion, Viral Nonstructural Proteins genetics, Virulence, Genome, Viral, Porcine Reproductive and Respiratory Syndrome virology, Porcine respiratory and reproductive syndrome virus genetics, Porcine respiratory and reproductive syndrome virus isolation & purification, RNA, Viral genetics, Sequence Analysis, DNA, Swine virology
Abstract

A highly pathogenic strain of porcine reproductive and respiratory syndrome virus (PRRSV), characterized by a discontinuous 30-amino-acid deletion in its Nsp2-coding region, has been emerging in China since 2006. Here, we report the complete genomic sequence of two novel Chinese virulent PRRSV variants with additional NSP2-gene deletions, which will help us understand the molecular and evolutionary characteristics of PRRSV in Asia.

Published
2012
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