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ObjectivesThis study proposes a structural strain test method for investigating the buckling pressure of a composite shell in a high hydrostatic pressure water environment and the effects of different initial defects. MethodsA high hydrostatic pressure test is conducted on a composite shell, and the strain of the structure is measured under 15 MPa hydrostatic pressure using the deep-water 3D-DIC (digital image correlation) method based on a high pressure shield. The results are compared to numerical simulations to confirm the feasibility of the 3D-DIC test system in a high hydrostatic pressure environment and verify the accuracy of the numerical method. Based on the numerical simulation method, research is then conducted on the buckling behavior of the composite shell. ResultsThe average error between the experimental results and numerical simulation results is found to be 7.2%. In addition, the ultimate load capacity of the composite shell is found to be in an almost quadratic function relationship to the size of the added geometric defects in different modes, and the average gradient of change in the ultimate load capacity is 1.48−2.12 MPa/mm in the first order mode and 1−4.32 MPa/mm in the third order mode. Conclusions3D-DIC test technology allows for the accurate strain measurement of composite shells in high hydrostatic pressure environments. Moreover, in such an environment, geometric defects largely affect the ultimate load capacity of the composite shell, while third-order modes are affected to a greater extent than first-order modes.

Abstract Background PDZ-binding kinase/T-lymphokine-activated killer cell-derived protein kinase (PBK/TOPK) is a potential prognostic indicator for patients with breast cancer. The objective of the present study was to explore the relationship between PBK/TOPK expression and clinicopathological indicators as well as the survival of patients with breast cancer. Methods Immunohistochemical staining was used to detect the expression of PBK/TOPK in 202 cases of breast cancer tissues. The relationship between PBK/TOPK and clinicopathological parameters was evaluated using Spearman’s rank-order correlation. The difference in PBK/TOPK expression among different molecular types was analyzed with the chi-square test. Kaplan-Meier analysis was used to create a survival curve and the log rank test was used to analyze the overall survival (OS) and disease-free survival (DFS). Prognostic correlation was assessed using univariate and multivariate Cox regression analyses. Results Among 202 breast cancer samples, PBK/TOPK was expressed (“+” and “++”) in 182 samples (90.1%). In addition, the histological grade, TNM stages, lymph node metastasis, estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER-2), and Ki-67 were positively associated with PBK/TOPK expression. With regard to the molecular type, the expression of PBK/TOPK is different. The expression level of PBK/TOPK was negatively correlated with both the OS and DFS of breast cancer patients. The difference in the above results is meaningful (P < 0.05). Conclusions PBK/TOPK is overexpressed in breast cancer, and the expression is closely related to the clinicopathological characteristics of the disease. Breast cancer patients with high expression of PBK/TOPK have a poor prognosis. Therefore, healthcare providers can optimize breast cancer management using this indicator.

While water splitting in acid offers higher operational performances than in alkaline conditions, there are few high-activity, acid-stable oxygen evolution electrocatalysts. Here, authors examine electrochemical Li intercalation to improve the activity and stability of RuO2 for acidic water oxidation.

The traditional aircraft controller design is usually based on the off-line aerodynamic model. Due to the deviation of the off-line aerodynamic model, the flight quality is difficult to meet the requirements when the aircraft is flying in the real atmosphere. To solve this problem, this paper proposes a frequency domain identification-based improved adaptive nonlinear dynamic inversion (NDI) control method (FDI-ANDI). In this paper, an online recursive aerodynamic parameter identification method in the frequency domain is first designed, and then an adaptive dynamic inversion control method based on the online aerodynamic parameter identification results is established. Finally, aiming at the problem of the slow response speed of the NDI controller, an improved adaptive dynamic inversion control law is designed by using the method of series lead correction. Compared with the traditional control method, the adaptive dynamic inversion method based on online aerodynamic identification has stronger robustness and a faster response speed in the face of model uncertainty. The final simulation analysis shows that the method has a better control effect than the traditional control method.

The dark brown anaerobic digestion piggery effluent (ADPE) with a large amount of ammonium generally needs high dilution before microalgae cultivation due to its inhibiting effects on algal growth. Due to the strong decolorization of fungi by degrading organic compounds in wastewater, the process-optimization integrated strategy of fungal decolorization of ADPE and subsequent microalgae cultivation with ammonium-tolerant strain may be a more reliable procedure to reduce the dilution ratio and enhance algal biomass production, and nutrient removal from ADPE. This study determined a suitable fungal strain for ADPE decolorization, which was isolated and screened from a local biogas plant, and identified using 26s rRNA gene sequence analysis. Subsequently, ADPE was pretreated by fungal decolorization to make low-diluted ADPE suitable for the algal growth, and conditions of microalgae cultivation were optimized to achieve maximum algal yield and nutrient removal from the pretreated ADPE. The results showed one promising locally isolated fungal strain, Nanchang University-27, which was selected out of three candidates and identified as Lichtheimia ornata, presenting a high decolorization to ADPE through fungal pretreatment. Five-fold low-diluted ADPE pretreated by L. ornata was the most suitable medium for the algal growth at an initial concentration of ammonium nitrogen of 380 mg L−1 in all dilution treatments. Initial optical density of 0.3 and pH of 9.0 were optimal culture conditions for the algal strain to provide the maximum algal yield (optical density = 2.1) and nutrient removal (88%, 58%, 65%, and 77% for the removal rates of ammonium nitrogen, total nitrogen, total phosphorus, and chemical oxygen demand, respectively) from the pretreated ADPE. This study demonstrated that fungal decolorization and subsequent microalgae cultivation could be a promising approach to algal biomass production and nutrient removal from ADPE.

This paper explores the use of sequential draw probabilities for two "players", banking and enterprise sectors, to predict impending financial crisis. Financial crises typically follow cyclical patterns where positive and negative turns are generated by underlying economic mechanisms. We envisage risk transfer between the financial sector and the real economy as one such mechanism. An analysis of draw probabilities calculated for differences in essential measures of fiscal risk, from the banking and enterprise sectors, reveals a intriguing phenomenon that may be used to assess the proximity (or remoteness) and possible intensity of financial crisis. Specifically, we provide evidence that draw probabilities comparing the competitive differences in Return of Equity between banks and enterprises, can be helpful in gauging asymmetry of risk; where such asymmetry has, historically, preceded grim financial outcomes.

Human mitochondrial pyruvate carriers (hMPCs), which are required for the uptake of pyruvate into mitochondria, are associated with several metabolic diseases, including type 2 diabetes and various cancers. Yeast MPC was recently demonstrated to form a functional unit of heterodimers. However, human MPC-1 (hMPC-1) and MPC-2 (hMPC-2) have not yet been individually isolated for their detailed characterization, in particular in terms of their structural and functional properties, namely, whether they exist as homo- or heterodimers. In this study, hMPC-1 and hMPC-2 were successfully isolated in micelles and they formed stable homodimers. However, the heterodimer state was found to be dominant when both hMPC-1 and hMPC-2 were present. In addition, as heterodimers, the molecules exhibited a higher binding capacity to both substrates and inhibitors, together with a larger structural stability than when they existed as homodimers. Taken together, our results demonstrated that the hetero-dimerization of hMPCs is the main functional unit of the pyruvate metabolism, providing a structural insight into the transport mechanisms of hMPCs.

HIV-1 integrase (HIV-1 IN) is an enzyme produced by the HIV-1 virus that integrates genetic material of the virus into the DNA of infected human cells. HIV-1 IN acts as a key component of the Retroviral Pre-Integration Complex (PIC). Protein dynamics could play an important role during the catalysis of HIV-1 IN; however, this process has not yet been fully elucidated. X-ray free electron laser (XFEL) together with nuclear magnetic resonance (NMR) could provide information regarding the dynamics during this catalysis reaction. Here, we report the non-cryogenic crystal structure of HIV-1 IN catalytic core domain at 2.5 Å using microcrystals in XFELs. Compared to the cryogenic structure at 2.1 Å using conventional synchrotron crystallography, there was a good agreement between the two structures, except for a catalytic triad formed by Asp64, Asp116, and Glu152 (DDE) and the lens epithelium-derived growth factor binding sites. The helix III region of the 140–153 residues near the active site and the DDE triad show a higher dynamic profile in the non-cryogenic structure, which is comparable to dynamics data obtained from NMR spectroscopy in solution state.

An analytical method based on the wave theory is proposed to calculate the pressure at the interfaces of coated plate subjected to underwater weak shock wave. The method is carried out to give analytical results by summing up the pressure increment, which can be calculated analytically, in time sequence. The results are in very good agreement with the finite element (FE) predictions for the coating case and Taylor’s results for the noncoating case, which validate the method that is suitable for underwater weak shock problem. On the other hand, Taylor’s results for the coating case are invalid, which indicates a potential application field for the method. The extension of the analytical method to q-layer systems and dissipation case is also outlined.