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BACKGROUND Rhamnolipids (RLS), well known as glycolipid biosurfactants, display low toxicity, high biodegradability, and strong antibacterial properties. This study was carried out to evaluate the use of RLS supplementation as a substitute for antibiotics, and particularly to evaluate its effects on growth performance, immunity, intestinal barrier function, and metabolome composition in broilers. RESULTS The RLS treatment improved the growth performance, immunity, and intestinal barrier function in broilers. The 16S rRNA sequencing revealed that the genus Alistipes was the dominant genus in broilers treated by RLS. An ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based metabolomic analysis indicated that the sphingolipid metabolism, glycine, serine, and threonine metabolism, the gycerophospholipid metabolism, and the tryptophan metabolism were changed in broilers that were treated with RLS. CONCLUSION l-Tryptophan may be the medium for RLS to regulate the growth and physiological metabolism. Rhamnolipids can be used as a potential alternative to antibiotics, with similar functions to antibiotics in the diet of broilers. The optimal level of supplemented RLS in the diet was 1000 mg kg-1 . © 2021 Society of Chemical Industry.

Grape seed is rich in vitamin E, flavonoids, and proanthocyanidins and has the potential to be used as an antibiotic substitute in broilers. We investigated the effects of grape seed proanthocyanidin extract (GSPE) on growth performance, immune responses, cecal microflora, and serum metabolism in early stage broilers. Data indicated that GSPE improved broiler growth performance by strengthening antioxidant capacity, enhancing immune responses, and increasing cecal short chain fatty acids. 16S rRNA sequencing indicated that GSPE changed the predominant cecal microflora and induced the metabolism of amino acids, lipids, and carbohydrates. An UPLC-Q-TOF/MS-based metabolomics analysis identified 23 serum metabolites (mainly related to lipid, amino acid, and alkaloid) were extremely changed by GSPE treatment. The correlations between the changes of cecal microflora and serum metabolites in birds fed with GSPE were analyzed. Hence, GSPE potentially provides active ingredients that may be used as antibiotic substitute and reduces environmental pollution by grape by-products.

The 26 November 2005 Jiujiang-Ruichang, Jiangxi, Ms 5.7 earthquake occurred in a seismotectonic setting of moderate earthquake. The northwest-trending Xiangfan-Guangji fault (XFG) does not enter into the epicenter vicinity, but the northeast-trending Ruichang-Wuning fault (RWF) as a regional fault extends to the epicenter nearby, appearing as the Ruichang basin and its marginal faults. Tilting of the Ruichang Basin (RCB) in the Quaternary was controlled by the RCB southeast-marginal, buried fault (RSMBF). Shallow geophysical survey reveals that the RSMBF caused an offset of the reflection layers. Drill hole columnar section demonstrates that there are about 10–12 m displacement in the lower section of the middle-Pleistocene Series along the RSMBF, but no disruption is found in the upper section of the middle-Pleistocene Series. The RSMBF not only has activity in the Quaternary, but also coincides with the nodal plane I from the focal mechanism of the Jiujiang-Ruichang Ms 5.7 earthquake. This evidence, including aftershock distribution and isoseismic lines, strongly suggests that the RSMBF might be the seismogenic tectonics. The RWF is discontinuous at the surface, and consists of three en echelon Quaternary basins, which are the Ruichang, Fanzhen and Wuning basins. Three moderate earthquakes, the Fanzhen ML 4.9 earthquake, the Yejiapu ML 4.1 earthquake and the Jiujiang-Ruichang Ms 5.7 earthquake, have happened in the basins since 1995. The seismogenic tectonics of the Jiujiang-Ruichang Ms 5.7 earthquake is not isolated, but may be controlled by the RWF at depth, the slip of which causes the accumulation of energy for earthquake occurrence.

Background Colibacillosis caused by enterotoxigenic Escherichia coli (E. coli) results in economic losses in the poultry industry. Antibiotics are usually used to control colibacillosis, however, E. coli has varying degrees of resistance to different antibiotics. Therefore the use of probiotics is becoming accepted as an alternative to antibiotics. In this study, we evaluated the effects of Clostridium butyricum (C. butyricum) on growth performance, immune response, intestinal barrier function, and digestive enzyme activity in broiler chickens challenged with Escherichia coli (E. coli) K88. Methods The chickens were randomly divided into four treatment groups for 28 days. Negative control treatment (NC) consisted of birds fed a basal diet without E. coli K88 challenge and positive control treatment (PC) consisted of birds fed a basal diet and challenged with E. coli K88. C. butyricum probiotic treatment (CB) consisted of birds fed a diet containing 2 × 107 cfu C. butyricum/kg of diet and challenged with E. coli K88. Colistin sulfate antibiotic treatment (CS) consisted of birds fed a diet containing 20 mg colistin sulfate/kg of diet and challenged with E. coli K88. Results The body weight (BW) and average day gain (ADG) in the broilers of CB group were higher (P