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This study aimed to elucidate the influence of various culture medium components, including carbon sources, nitrogen sources, inorganic salts, suspension agents, and temperature, on the mycelial growth characteristics of Phallus dongsun. Employing single-factor experiments and response surface methodology within glass Petri dishes, the research identified that carrot powder, soybean powder, and ZnSO4 notably enhanced the proliferation of aerial mycelium, significantly augmenting the growth rate of P. dongsun mycelium. The resultant mycelium was observed to be dense, robust, and fluffy in texture. In particular, ZnSO4 markedly accelerated the mycelium growth rate. Furthermore, xanthan gum was found to effectively modulate the medium’s viscosity, ensuring a stable suspension and facilitating nutrient equilibrium. The optimal cultivation temperature was determined to be 25°C, with mycelial growth ceasing below 5°C and mycelium perishing at temperatures exceeding 35°C. The optimal medium composition was established as follows: wheat starch 5 g/l, carrot powder 5 g/l, soybean powder 7.50 g/l, glucose 10 g/l, ZnSO4 0.71 g/l, NH4Cl 0.68 g/l, xanthan gum 0.5 g/l, and agar 20 g/l. Under these optimized conditions, the mycelium of P. dongsun exhibited a rapid growth rate (1.04 ± 0.14 mm/day), characterized by a thick, dense, and well-developed structure. This investigation provides a theoretical foundation for the conservation, strain selection, and breeding of P. dongsun.

Objective To investigate the risk factors of postoperative infection after open wedge high tibial osteotomy (OWHTO). Methods Clinical data of 114 patients who underwent primary OWHTO in a Class A tertiary hospital from August 2017 to October 2020 were collected and retrospectively analyzed, and all of them completed regular postoperative follow-up. The patients were divided into control group and infection group according to the presence or absence of postoperative infection. Univariate and logistic regression analyses were used to explore the risk factors of infection after OWHTO. Results Among the 114 patients included in the study, 31 cases had postoperative infection, of which 20 cases were controlled within 2 weeks after operation, and the symptoms of infection were eliminated in the other 11 cases about 4 weeks postoperatively. Multivariate logistic regression analysis showed that diabetes mellitus (P=0.001), smoking (P < 0.001), osteotomy clearance ≥10 mm (P=0.001), bone graft (P=0.001), and operation duration longer than 3 h (P < 0.001) were independent risk factors for postoperative infection (P < 0.05). Conclusion The patients with diabetes mellitus, smoking, osteotomy space ≥10 mm, intraoperative graft and operation time ≥3 h have a higher risk of infection after OWHTO.

Mycoplasma pneumonia (MPS), caused by Mycoplasma hyopneumoniae (Mhp), is a chronic, airborne respiratory disease that poses a significant threat to the global swine industry. The P97 and P46 proteins are major antigens of Mhp, with the R1 region of P97 possessing full adhesive capability. Studies have shown that the main antigenic regions of Mhp P42 and P65 proteins exhibit strong immunogenicity. In this study, we first linked the genes encoding P97R1 and P46 proteins to form the P97R1P65 gene and subsequently constructed three shuttle plasmids: pFBD-P97R1P46, pFBD-P97R1P46-p65, and pFBD-P65-P42. These proteins were expressed using the Bac to Bac system and formulated into subunit vaccines for mouse immunization. Mouse experiments indicated that the P97R1P46 + P65-P42 protein combination elicited higher levels of specific antibodies, IL-2, IL-4, and CD8+ T cells compared to other subunit vaccine groups, a finding further validated in subsequent mouse challenge protection experiments. Therefore, we utilized the MultiBac expression system to co-express P97R1P46, P65, and P42 proteins in the pFastMultibacDual vector for immunization experiments in piglets. The piglet immunization experiments demonstrated that the Mhp subunit vaccine prepared in this study could induce specific antibodies against Mhp, with the combination of P97R1P46, P65, and P42 proteins inducing the highest level of humoral immunity. This study provides valuable insights for the development of Mhp subunit vaccines. [ABSTRACT FROM AUTHOR]

Recently, the emergence of HP-PRRSV (Highly Pathogenic porcine reproductive and respiratory syndrome virus) and the exacerbation of mixed infections of PRRSV and PCV have resulted in significant economic losses for the Chinese pig industry. This study collected a total of 226 samples suspected of infection with the aforementioned viruses from diverse pig farms in seven urban districts of central and northern Guangdong Province between 2020 and 2022. The positive rates of PRRSV, PCV2, and PCV3 in the samples were 33.2%, 37.6%, and 7.5%, respectively, and there were various mixed-infection scenarios present in the samples. This study successfully isolated multiple strains of PRRSV2 and PCV2 from their positive samples, and obtained the gene sequences of six PCV3 (ORF1 + ORF2) from samples. The associated sequences obtained were subjected to bioinformatic analysis and revealed the following:Predominantly prevalent strains of PRRSV in Guangdong Province include HP-PRRSV and NADC30-like variants, whereas PCV2 is primarily represented by the 2b and 2d subtypes. Specifically, the amino acid variation patterns exhibited by the PRRSV GP5 and NSP2 proteins of the strains sg_2108, qy_2008, and fs_2108 under environmental selective pressure are remarkably similar to the characteristics of Highly Pathogenic PRRSV; thus, it is inferred that they may possess higher virulence. The detected PCV3 strains were predominantly concentrated within the PCV3a-IM branch. All PRRSV strains involved in this study are wild-type-PRRSV (wt-PRRSV), comprising three recombinant strains and seven highly virulent strains. Among these strains, the ORF1a gene exhibited the highest variability in their genomes. Environmental selective pressure may enhance the virulence and immune evasion capabilities of PRRSV and drive mutations in the Cap proteins of PCV2 and PCV3. Conversely, PCV2 and PCV3 strains demonstrated greater stability in genetic evolution. In conclusion, this study enhances the epidemiological data regarding PRRSV, PCV2, and PCV3 in Guangdong Province, China, and is significant for the surveillance, prevention, and active control of these three diseases. [ABSTRACT FROM AUTHOR]

Spectrum map is a database that stores multidimensional representations of spectrum situation information. It provides support for spectrum sensing and endows wireless communication networks with intelligence. However, the ubiquitous deployment of monitoring devices leads to huge costs of operation and maintenance. It indicates that an approach is needed to reduce the number of monitoring devices, but prevent the degradation of data granularity. Therefore, this paper focuses on the accurate construction of the spectrum map. It aims to infer the fine‐grained spectrum situation of the target region based on coarse‐grained observation. In order to solve this problem, an inference framework based on deep residual network is developed in this paper. In the case of rule deployment for sensing nodes, it adopts the idea of super resolution to improve the accuracy of the spectrum map. The framework is composed of two major parts: an inference network, which generates fine‐grained spectrum maps from coarse‐grained counterparts by using feature extraction module and upsampling construction module; and a fusion network, which considers the influence of environmental factors to further improve the performance. A large number of experiments on simulated datasets verify the effectiveness of the proposed method. [ABSTRACT FROM AUTHOR]

Gas plays a crucial role in cell culture as cells require a specific gas environment to maintain their growth, reproduction, and function. Here, we propose a gas supply system for tri-gas multi-channel cell incubators to meet the specific needs of various cells. The system utilizes a circulating gas supply method powered by air pumps for each chamber. Gas inflow from the cylinder is independently controlled by Mass Flow Controllers (MFCs), and a quantitative step-by-step adjustment control strategy is employed to calculate the volume of different gases being introduced. Through mixing simulations and experiments, we identified the SV static mixer with an L/D ratio of 2.5 as the optimal choice. To evaluate the concentration accuracy and gas consumption of the gas system, we conduct gas mixing and distribution experiments under different conditions. The results show that the system could achieve a concentration range of 0–100% for O2 with an accuracy of ±0.5%, and a concentration range of 0–10% for CO2 with an accuracy of ±0.1%. The daily gas consumption during cultivation is 3570 mL of N2, 330 mL of CO2, and 115 mL of O2, significantly lower than conventional incubators. Overall, our system can effectively manage dynamic gas concentration changes, particularly in high O2 concentration environments. It offers advantages such as low gas consumption, a wide concentration range, and high accuracy compared to existing incubators. [ABSTRACT FROM AUTHOR]

The energy efficiency of vehicles is a crucial challenge relating to sustainable energy preservation and regeneration methods. Regenerative breaking has proven feasible, and there is interest in whether harvesting energy from a vehicle's suspension is similarly feasible. We here provide methods for estimating the amount of power that can be regenerated from the suspension for given vehicle and road parameters. We show that a reasonable road model is a generalised Gaussian process known as AR(1). Using this model, we can derive the key equation used in the ISO 8608 standard for measuring road roughness, such that the AR(1) parameters can be related to the measured road roughness data. We find that the road roughness coefficient of ISO 8608 and the diffusion coefficient of the AR(1) road are equal up to a factor. We provide an analytical expression for the maximum amount of power that can be generated for given road and car parameters, derived via Fourier analysis. We further model harvesting from large bumps using Simulink. These results help to estimate the potential power output given the measured road data. [ABSTRACT FROM AUTHOR]

The occurrence of classical swine fever (CSF) poses a significant threat to the global swine industry. Developing an effective and safe vaccine is crucial for preventing and controlling CSF. Here, we constructed self-assembled ferritin nanoparticles fused with the classical swine fever virus (CSFV) E2 protein and a derived B cell epitope (Fe-E2B) using a baculovirus expression system (BVES), demonstrating enhanced immunogenicity. Furthermore, we provide a detailed evaluation of the immunological efficacy of the FeE2B in rabbits. The results showed that robust and sustained antibody responses were detected in rabbits immunized with the Fe-E2B nanoparticle vaccine, comparable to those elicited by commercially available vaccines. Additionally, we demonstrated that the vaccine effectively activated crucial immune factors IFN-γ and IL-4 in vivo, increasing their levels by 1.41-fold and 1.39-fold, respectively. Immunization with Fe-E2B enabled rabbits to avoid viremia and stereotypic fever after CSFV challenge. In conclusion, this study highlights the potential of ferritin nanoparticles as antigen-presenting carriers to induce robust immune responses, proposing a candidate vaccine strategy for the prevention and control of CSF. [ABSTRACT FROM AUTHOR]

BACKGROUND: High-quality control of the gas environment in incubators is crucial for in vitro embryo development, which requires high accuracy, fast recovery, and low gas consumption. OBJECTIVE: In this study, we propose a novel gas mixing and distribution system and method as an alternative solution for multi-chamber embryo incubators. METHODS: The system-based embryo incubator enables a controllable gas circulation process and a quantitative supply of CO2 and N2. To determine the optimal parameters for the mixing time and flow rate of the circulated gases, we conducted contrast experiments on the system-based incubator. To evaluate the performance of the gas system in the incubator, we conducted tests under four different initial conditions, simulating various practical application scenarios. Furthermore, we performed a mouse embryo assay to assess the system's effectiveness. RESULTS: The results show that the system achieved a gas concentration accuracy of ± 0.2% (volume fraction) after stabilization, a minimum recovery time of 5 minutes, an average consumption of 8.9 L/d for N2 and 0.83 L/d for CO2 during routine operation, and a blastocyst rate exceeding 90% observed after 96 hours of culture in the incubator. CONCLUSION: The system and method demonstrate a significant advantage in terms of low gas consumption compared to existing incubators, while still maintaining high accuracy and fast recovery. [ABSTRACT FROM AUTHOR]

We have looked into cobalt disilicide (CoSi2) as a potential building block for superconducting quantum circuits. In order to achieve this, we annealed a thin layer of Co to create microwave cavities with thickness of d = 10–105 nm from CoSi2 embedded in the silicon substrate. The cavity properties were measured as a function of temperature and power. In the films measuring 10 and 25 nm, we find a significant kinetic inductance LK with a non-BCS power-law variation δLK ∝ T4.3±0.2 at low temperatures. The quality factor of the studied microwave resonances varied from 3 × 103 (d = 10 nm) to ∼5 × 104 (d = 105 nm) and increased as d(A − log d) with thickness, with two-level systems having very little effect. The power dependence of kinetic inductance was analyzed in terms of heat flow due to electron–phonon coupling, which was found to be stronger than estimated for heat relaxation by regular quasiparticles. [ABSTRACT FROM AUTHOR]