Search

International audience; In this work, we present a pre-analytical module based on the combination of microfluidic and electroacoustic technologies to intercept and sort submicron biological particles. It is composed by assembling a lithium niobate (LN) substrate for acoustic function and a micromachined glass substrate for microfluidic channel. The interference between the two surface acoustic waves (SAWs) generated by inter digital transducers (IDTs) create a distribution of an acoustic radiation force (ARF). This force affects differently particles depending on their physical proprieties. The device is powered by an electronic circuit with a phase shifter to move the node of the standing surface acoustic wave (SSAW) along the channel width. When the device is powered at resonance frequency, experience has shown a submicron particles alignement at a fixed position along the channel. By shifting the SSAW phase, the particles are driven to one of the three channel outlets.

International audience; Manipulating micro and nano-biological particles like extracellular vehicles (EVs), without extracting them from their biological media, presents a big challenge for diagnosis purposes. Here we present the design and fabrication of a sorting device based on the combination of microfluidic and electroacoustic modules that is capable of aligning and sorting submicron biological particles according to their size, compressibility or mass density, all in a tunable way. The device relies on a lithium niobate (LN) substrate to generate acoustic waves assembled with a micromachined glass layer for microfluidic circuits. The interference between the two surface acoustic waves (SAWs) generated by interdigitated transducers (IDTs) create a distribution of an acoustic radiation force (ARF). This force affects differently particles depending on their physical proprieties. The device is powered by an electronic circuit with a phase shifter to move the node of the standing surface acoustic wave (SSAW) along the channel width. When the device is powered at resonance frequency of the IDTs, experiment shows submicron particles alignment along the channel. By shifting the electrical signal between the two IDTs we can translate the pressure node at any targeted position in the channel width. The particles are then driven to one selected outlet.

International audience; Extracellular vesicles1 (EVs, among them exosomes and microvesicles) and subcellular species can be found in all biological fluids and present an increasing potential for biological applications. Succeeding in separating these vesicles from their physiological environment and analysing different subsets is of primary importance. The real challenge, for this kind of particles, is to find a reliable method, which works in an easy and a simple way, and that is reproducible and fast. For this purpose acoustofluidic particles-sorting devices have been demonstrated2,3. We propose a combination of several complementary approaches to isolate, detect and analyse biological microparticles. First, we developed an acoustofluidic device enabling to separate particles from a complex sample by using stationary acoustic waves. Secondly, we propose an other device, which consists in a fluidic chamber integrating a home-made gold biochip4, with a tailored design and allowing the capture of vesicles subsets on specific biofunctionalized spots of antibodies and receptors. Then different analytical technics could be used, firstly in solution to characterize sorted vesicles, by tuneable resistive pulse analysis (TRPS), and then on the biochip to characterize the size and phenotype of the captured material, by atomic force microscopy (AFM) and mass spectrometry (MS) respectively. These methods allow obtaining important information about these nano-vesicles which have high potential and relevancy in clinical diagnostics and early treatment of numerous diseases.

The aim of the project is to develop a plasmon-acoustic device for the real time detection of biomolecular interactions, in the prospect of cancer markers detection in blood samples from patients. In the context of early diagnosis, the detection of infinitesimal quantities of biomarkers is possible thanks to the breakdown of the equilibrium state of biochemical reactions. This could be obtained by the activation of the biological media using a system composed of a microdevice for generating acoustic waves coupled with a fluidic network. The latter system will be integrated on a detection device based on surface plasmon resonance. Here, we present design, realization and characterization of the acoustic activation device fabricated on silicon thanks to technological facilities of MIMENTO and ARCEN platforms