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Lentinula edodes (shiitake mushroom) is the medicinal macro-fungus popular in Japan and China. It is the second most well- known edible fungus in the global market after Agaricus bisporus Because of presence of dietary fibers, vitamins, proteins, minerals and carbohydrates, shiitake mushroom have significant nutritional worth. It is commonly cultivated in winter season and known as “elixir of life”. Bioactive compounds such as polysaccharides, amino acid derivatives, sterols, glycoproteins, chitin and sulfurous compounds are all extracted from shiitake mushrooms. Some of these are lentinans, eritadenine and KS-2-α- mannan peptide etc. Shiitake mushroom is used therapeutically to cure cancers, cardiovascular disorders, viral and fungal infections, hepatitis, hypertension and hepatic disorders. Shiitake mushroom have also grown in popularity as a result of their unusual and well-liked flavor.

Background: Hepatitis C Virus is a member of flaviviridae family in hepacivirus genome. HCV effect liver that develop in CHC contagion in maximum patients. About 160 million individuals are infected by HCV contagion that leads Long-lasting hepatitis C, liver cirrhosis, hepatocellular carcinoma. Direct damaged hepatic cells by HCV cause CHC and Chronic Hepatitis C leads lipid accumulation in liver. Objective: To evaluate the lipid peroxidation and Hepatic profile in hepatitis C patients receiving ribavirin and sofosbuvir. Methodology: Venous blood samples (5.0 ml) of 50 diagnosed Hepatitis C patients receiving sofosbuvir and ribavirin and 50 Blood samples (5.0 ml) of Healthy individuals were taken in clotted gel vials. Blood serum was further tested for estimation of Malondialdehyde (MDA), Nitric Oxide (NO), Advanced Glycation End products (AGE’s), Serum Liver Functions tests (LFTs), Lipid Profile, Urea and Creatinine by using spectrophotometer. Results: Serum MDA level significantly elevate (8.60) in diseased individuals receiving sofosbuvir and ribavirin than control (1.3) and NO level significantly increase in patients (35.21) as compare to healthy individuals (6.41). Serum hepatic profile status reveals that value of ALT significantly rises in disease persons (44.56) that control individuals (36.22). Serum AST level increase significantly in diseased individuals (40.33) as compare to healthy individuals (29.81). All parameters showed significant statistically (P = 0.000 < 0.05). Conclusions: Due to high lipid peroxidation, serum MDA level and NO level elevated remarkably in Hepatitis C patients that is highly significant. AGE’s level and Creatinine level decreased in Hepatitis C patients. ALT, AST, ALP, Total Bilirubin, Urea, Cholesterols and Triglyceride level increased in HCV patients that may leads to the progression of disease. Key Words: HCV, Ribavirin, Sofosbuvir, ALT, AST, MDA

Background: Ovariancancerrefers to the growth of any cancerous cells in the ovaries. Most often ovarian cancer arises from the epithelium (outer lining) of the ovary.Ovarian cancer considered as the major reason of death in gynecological malignancies. The ovaries are two small organs located on either side of the uterus in a woman’s body. Methodology: 5.0 ml blood sample of 50 ovarian cancer patients and 50 healthy persons was collected in EDTA vials from oncology department of Mayo and Jinnah Hospital Lahore. MDA, CAT, GSH, SOD, Micronutrients, serum Electrolytes were estimated Results: Serum MDA level in ovarian malignancy patients is remarkably inflated (8.42 ± 0.53) whereas there level in healthy individuals was extremely low (2.25±.24). In patients the level of GSH reduced (0.12 ±0.14) as compared to normal individual (6.35 ± 0.17). The CAT level is moderately decreased in patients (0.60±0.52) as compared to healthy individual is high (4.16±1.06). Ovarian cancer patients have remarkably deficient vitamin A level (2 .55±0.17) while its level was extremely high in healthy subjects (7.15±0.47). So it depicts that ovarian cancer patients shows the highly significant behavior (p꞊0.000).Conclusion: A fine balance between oxidants (ROS production) and antioxidants is predominant for the general defensive capacity of cells in contrast to oxidative damage. MDA level in ovarian malignancy patients is remarkably inflated due to high lipid peroxidation mechanism. Key Words: SOD, MDA, GSH, CAT, Ovarian Cancer&rsquo

Background: Dopaminergic movement in the prefrontal cortex are related with the shortfall side effects and psychological impedance of schizophrenia and that expanded action in the sub cortex is related with the insane manifestations of schizophrenia. A partial employment of glutamatergic transmission in oxytocin mediated choking of dopaminergic psycho stimulant induced practices has in like manner been proposed. Objective: To determine the Lipid Peroxidation status in schizophrenic patients receiving anti-psychotic drugs. Methodology: Comparative Study. Fourty patients of Schizophrenia and Fourty age and sex-matched healthy individuals were eligible for inclusion in the study at Mental Hospital Lahore. 5.0 ml blood sample was taken from each individual and subjected to centrifuge at 3500-4500 rpm for 10-15 minutes for the separation of serum. Oxidative stress biomarkers (NO, MDA, AOPP and AGE’s) and serum electrolyte profile, Serum LFT’s, Serum RFT’s and serum Lipid Profile were estimated. Results: NO level in schizophrenic patients is (0.17 ± 0.10), while NO level in sound people (12.3±2.05) Data also shows that NO is significant statistically (P = 0.000MDA level in schizophrenic patients is (0.23±0.08), while MDA level in healthy individuals (1.27±0.28). Information additionally demonstrates that MDA is critical factually (P = 0.000Na+ level in schizophrenic patient’s is (78.93±59.46), while Na+ level in healthy persons (141.5±2.05). Data other than presentations that Na+ is key truly (P = 0.000 Conclusion: Present study concluded that serum total protein, sodium (Na+), NO, AGE,s and AOPP level declined remarkably which is the cause for the progression of disease. Key Words: NO, MDA, AGE’s, AOPP, Lipid Peroxidation.

The objective of this study was to discover the scientific source these plants have much inhibitory effects against Vero cell lines. Medicinal plants have secondary metabolites in which more quality against many type of disordered and like flavonoids, phenol, taninns, and some other that have very good and special properties are occur them. DPPH assay screen test on the TLC shows very much anticancer activity. MTT assay proved that these plants are abundant inhibitory activity against VERO cell liens cell. These shows we can reduce that death rate of cancer and also control that cancer that are spared in other body organ and also inhibited the disorder. Key Words: TLC, DPPH, MTT, Anti-Cancer, Phytochemicals.