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Seventy-eight men with symptoms of chronic or subacute prostatitis were enrolled. Investigations for the presence of Chlamydia trachomatis in urethral swabs were carried out. The expressed prostatic secretions were additionally examined for Mycoplasma hominis, Ureaplasma urealyticum, Gardnerella vaginalis, other gram-negative and gram-positive bacteria, Trichomonas vaginalis, yeast-like fungi and leucocyte count. Furthermore, all patients were evaluated for the presence of serum anti-chlamydial IgG antibodies. Signs of inflammation on the basis of the count of leucocytes per hpf in the prostatic secretions were detected in 42 patients (group I). Prostatodynia was found in the remaining 36 men (group II). In group I, chlamydial antigen was detected in the urethra and expressed prostatic secretions (EPS) in 6 (14.3%) and 9 (21.4%) patients, respectively. No evidence of current chlamydial infection was found in group II. The presence of serum anti-chlamydial IgG antibodies was demonstrated in 13/42 (30.9%) patients with prostatitis and in 3/36 (8.3%) patients with prostatodynia (P < 0.01). The results suggest that chlamydia may be one of the causative agents of chronic prostatitis. [ABSTRACT FROM AUTHOR]

Purpose: The study objective was to evaluate the prevalence of Chlamydia trachomatis (C. trachomatis) infection in women with and without pathological lesions in the uterine cervix. Material and methods: A total of 120 patients, aged 15-57 (mean age 29), recruited for the study, were referred by gynaecological clinics in the Podlasie province. Gynaecological examinations confirmed cervicitis accompanied by erosions in 75 patients (group I) and cervicitis alone in 45 women (group II). The comparative group (control) consisted of 35 women, aged 16-48 years (mean age 29), who had no clinical symptoms or pathological lesions in the cervix. Direct immunofluorescence tests (MicroTrack, Syva) or polymerase chain reaction assays (PCR, Roche) were used to detect C. trachomatis infection in cervical samples. Antichlamydial IgG antibodies in the serum were determined using an immunoenzymatic assay (C. trachomatis IgG, EIA medac). Two-frequencies test was used for the statistical analysis of results. P values of <0.05 were considered statistically significant. Results: In the direct tests, C. trachomatis infection was found in group I in 9/75 women (12.2%), in group II in 9/45 (20%) and in the comparative group in 1/35 (2.9%) (group I vs control p>0.1252; group II vs control p<0.025). IgG specific antibodies were detected in group I in 17/49 patients (34.7%), in group II in 5/18 (27.8%) and in the comparative group in 2/35 (5.7%) women (group I vs control p<0.0022; group II vs control p<0.0319). Conclusions: Our results show a higher prevalence of C. trachomatis infection in female patients with cervical lesions as compared to unaffected women, thus suggesting that diagnostic tests for C. trachomatis infection should be included in the screening programs for women. [ABSTRACT FROM AUTHOR]

Introduction: The aim of the study was to evaluate the frequency of occurrence of Chlamydia trachomatis (C.t.) DNA in the prostate material in the group of individuals with the chronic prostatitis., Material and Methods: The study included 65 males aged between 47 and 68 years of age, reporting for transrectal prostate biopsy because of the elevated serum prostate-specific antigen concentration and/or abnormalities detected in prostate palpation per rectum. The urethral smear collection was performed in all the patients in order to detect C.t. DNA. After that, the transrectal prostate biopsy was performed (histopathology tests, C.t. DNA). Additionally, the levels of anti-C.t. IgG antibodies and anti-C.t. IgA antibodies were checked in the serum. The DNA isolation from prostate specimens was conducted with the use of the Chelex method, while the C.t. DNA detection - with the ligase chain reaction. Specific antibodies were detected with the use of the ELISA method., Results: C.t. DNA in the prostate gland was found in 7 out of 65 men (10.8%). In urethral smear, C.t. was found in none of the individuals. Anti-C.t. IgA antibodies were detected in the serum of 16/65 (24.6%), while anti-C.t. IgG antibodies in 6/65 (9.2%) of the examined males. IgA antibodies were found in two and IgG in one out of the 7 men who had C.t. infection in the prostate., Conclusions: The presence of C.t. DNA in the prostate gland may be indicative of the role of chlamydia in the development of chronic prostatitis., Competing Interests: The authors declare no conflicts of interest., (Copyright by Polish Urological Association.)

Objectives: The aim of the study was to evaluate the incidence of Chlamydia trachomatis in patients with reactive arthritis (ReA) within the area of the Podlaskie province (north eastern Poland)., Material and Methods: The study concerned 323 patients including 132 women and 191 men diagnosed with ReA. The material for C. trachomatis was collected from the urethra in men and the cervical canal in women. Also, every patient was tested for the presence of anti-C. trachomatis IgG class antibodies, while 121 individuals were additionally tested for IgA class antibodies. In the direct studies, the direct immunofluorescence (DIF) method or polymerase chain reaction (PCR) was used. The immunoenzymatic method was used to detect anti-C. trachomatis antibodies. The control group in the case of direct studies comprised 125 individuals, while in the case of serology research it included 127 (IgG) and 109 (IgA) persons., Results: Chlamydia trachomatis infection in the urethral and cervical smears was found in 42 patients (13.0%) including 20 women (15.2%) and 22 men (11.5%). In the control group chlamydia was detected in 3 patients (2.4%) including 4% of women and 2% of men. IgA class antibodies were present in 10/121 (8.3%) patients, similarly in women and in men (8.2% and 8.3% respectively). In the control group the specific IgA class antibodies were found in 3/85 patients (3.5%). Anti-C. trachomatis IgG antibodies were found in 70/323 patients (21.7%), similarly in men and women., Conclusions: Chlamydia trachomatis is a common bacterial factor observed in the genitourinary system of patients with ReA. The outcomes of studies within the Podlaskie province indicate less frequent presence of chlamydial infection compared with Dolnośląskie province.No correlations between detecting the presence of C. trachomatis in the urogenital tract and the presence of specific antibodies in the serum of ReA patients were observed.Concurrent direct studies of the urogenital tract and a serological blood test increase the chance of detecting C. trachomatis infection.

Introduction: The aim of the study was to determine the numbers of polymorphonuclear (PMN) leukocytes and PMN elastase and citric acid concentrations in chronic prostatitis patients regardless of etiology and in those with Chlamydia trachomatis infection of the prostate gland., Materials and Methods: The study involved 46 patients with chronic prostatitis. Expressed prostatic secretions (EPS) were obtained to determine the leukocyte count, PMN elastase (ELISA) and citric acid concentrations (UV method), and the occurrence of C. trachomatis infection (ligase chain reaction)., Results: Increased PMN cell counts (> or =10 per high-power field) were found in 73.9% of patients and increased PMN elastase concentration (<250 ng/ml) in 78.3%. In 44.4% of the patients the elastase concentration indicated moderate (250-1000 ng/ml) and in 55.6% acute infection (> or =1000 ng/ml). Decreased citric acid concentration (<18.12 mg/ml) in the EPS was found in 65.2% of the men. C. trachomatis prostate infection was detected in 17.4% of the patients and all of these men had higher inflammation parameters and lower citric acid concentrations., Conclusions: C. trachomatis prostate inflammation was accompanied by an increase in inflammation markers and a decrease in citric acid concentration.

Introduction: The aim of the study was to evaluate the correlation between the presence of anti-C. trachomatis (C.t.) antibodies in serum and expressed prostatic secretions (EPS) and the concentration of citric acid in patients with chronic prostatitis., Materials and Methods: The study involved 34 men with chronic prostatitis. The leukocyte count, presence of anti-C.t. antibodies (IgA, IgG), and citric acid concentration were determined in the EPS. The serum was examined for IgM, IgA, and IgG anti-C.t. antibodies. Specific antibodies were determined using the EIA method. The concentration of citric acid was measured using the ultraviolet method., Results: Inflammation of the prostate (210 PMN) was found in 61.8% of the patients. A reduction in citric acid concentration in the EPS was detected in 58.8% of the men. Specific serum antibodies were detected in 58.8% of the patients, including 23.5% with IgM, 32.4% with IgA, and 44.1% with IgG. In all patients, serum IgM and IgA antibody titers were low, while those of IgG antibodies were strongly positive in 46.7% of the patients. Anti-C.t. antibodies in the EPS were detected in 44.1% of the patients, including 32.4% with IgA and 35.3% with IgG. In contrast to serum, the titers of IgG antibodies in the EPS were low in all the patients, while those of IgA were strongly positive in 54.5% of cases. In patients with positive serological outcomes, 85% had reduced concentrations of citric acid., Conclusions: The occurrence of anti-C.t. antibodies is usually accompanied by a decrease in the concentration of citric acid in the prostatic secretion.

Purpose: The aim of the study was to assess the incidence of Ureaplasma urealyticum (U. urealyticum) and Mycoplasma hominis (M. hominis) infection in women with urogenital diseases., Material and Methods: M. hominis and U. urealyticum was assessed in 541 women from gynaecological and STD outpatient clinics, aged 18-55 years. A Mycoplasma IST 2 kit was used for biochemical determination of mycoplasmal infections (BioMerieux). Additionally, 248 of patients were examined for Chlamydia trachomatis (C. trachomatis), Trichomonas vaginalis (T. vaginalis) and Candida albicans (C. albicans) infection. C. trachomatis was detected by direct immunofluorescence method. The standard culture methods (Biomed) were applied to detect T. vaginalis and C. albicans., Results: U. urealyticum was detected in 161 (29.8%), and M. hominis in 20 (3.7%) women. U. urealyticum infection alone was observed in 37/79 (46.8%), and 1/8 (12.5%) patient had only M. hominis infection. The U. urealyticum infection showed most frequent coexistence with C. albicans (29.1%), and less frequent with C. trachomatis (13.9%) and M. hominis infection (10.1%). The highest percentage of mycoplasma-positive cultures was found in patients of STD clinic and in infertile women. In patients with ureaplasmal infection only the most common clinical symptom was vaginal discharge and vulval/vaginal irritation. In 8.1% of the women, the course of U urealyticum infection was asymptomatic., Conclusions: The incidence rate of genitourinary infections due to U urealyticum was significantly higher as compared to M. hominis infection. Sexual mycoplasmal infections were most frequently reported in the group of patients of STD clinic and correlated with age and sexual activity.

Purpose: The aim of this study was to estimate the incidence of M. hominis and U. urealyticum infections among men with urethritis and its complications., Material and Methods: Material for analysis were urethral swabs and EPS. Mycoplasma IST 2 kit was applied to diagnose mycoplasmal infections. All patients were additionaly tested for C. trachomatis, C. albicans and T. vaginalis and Gram stain specimens were obtained to identify the presence of PMN., Results: U. urealyticum was detected in 57/390 (14.6%), and M. hominis in 4/390 (1%) men. Exclusive U. urealyticum infection was found in 45 (11.5%) men, and only 2 patients had exclusive M. hominis infection. U. urealyticum infection the most frequently coexisted with C. trachomatis--5 (8.8%), next with C. albicans--4 (7%) and M. hominis--2 (3.5%) infections. Mycoplasmal infections were the most frequently found in patients aged 30 to 39 (35.1%) diagnosed with epididymitis (29.2%). The most commonly reported symptom was dysuria., Conclusions: U. urealyticum is the common pathogen among men with urethritis and its complications. The most common symptoms in U urealyticum patients were: dysuria, hypogastric pains and urethrorrhoea, however, clinical symptoms are not frequently observed.

Introduction: The objective of the study was to assess the relationship between Chlamydia trachomatis (C.t.) infection of the prostate and the concentration of citric acid., Materials and Methods: The study involved 60 patients with chronic prostatitis (NIH III). Urethral swabs and expressed prostatic secretions (EPS) were collected for analysis. The urethral swabs were tested for PMNs and the presence of Neisseria gonorrhoeae and C.t., while the EPS were analyzed to determine PMN count, C.t., and citric acid concentration. The DFA or LCR method was used for C.t. diagnosis. The concentration of citric acid was measured using the UV method., Results: Inflammation of the prostate (PMNs >/=10/field) was diagnosed in 58.3 of the patients. C.t. infection was found in 20%, including 8.3% with only the urethra affected and 10% with only the prostate. One patient had both the urethra and the prostate infected. A reduction in the concentration of citric acid in EPS was observed in 56.7% of the men. In 88.2% of the patients, reduced citric acid concentration was accompanied by an elevated PMN count in the EPS. All patients with C.t. infection of the prostate showed a reduced concentration of citric acid. In five patients with urethral infection, lack of a decrease in this parameter was noted in one. In all the patients with chlamydial infection, irrespective of localization, a high PMN count was observed in the EPS., Conclusions: Determination of the concentration of citric acid in the prostatic fluid is a good indicator of prostatitis. C.t. infection of the prostate gland is accompanied by a decrease in the concentration of citric acid.

In the literature from recent years it is stressed the significant epidemiological and clinical role of the sexually transmitted infections caused by so called "new generation" pathogens including mycoplasmas. The paper gives a review of the current literature concerning the epidemiology, clinical presentation, diagnosis and therapy of the infections of urogenital tract caused by mycoplasmas.

Chronic inflammation has long been linked to cancers with an infectious etiology. Subclinical intraprostatic inflammation is a frequent finding in prostatic specimens obtained during surgical procedures. Recent studies using biomolecular (amplification) methods showed that the incidence of cryptic bacterial infections of prostate gland may be underestimated. We present the case of 66 years old man with no symptoms of prostatitis in whom chronic Chlamydia trachomatis infection was diagnosed based on the patient's medical history and detection of chlamydial DNA in prostate biopsy specimen by means of ligase chain reaction. Two years later the patient was diagnosed with prostate cancer on repeated prostate biopsy.

The drug hypersensitivity syndrome (DHS) is a drug-induced reaction of an extremely severe clinical course and serious prognosis. It poses major diagnostic problems and is frequently unrecognized by clinicians. Based on literature data, the authors have presented the information on epidemiology, etiology, pathogenesis, clinical picture, diagnostics and treatment of DHS. This is the first report in Poland concerning this clinical interdisciplinary syndrome.

Aim: The aim of the study was to evaluate the prevalence of Chlamydia trachomatis (C. trachomatis) infection in women with adverse pregnancy outcomes., Material and Method: 258 patients aged 18-43 yrs were enrolled into the study. Among them, 162 women have had spontaneous abortions in the past (group A), 81 had history of intrauterine death of the foetus (group B) and 15 women had experienced preterm deliveries (group C). The control group (group D) included 131 women who were in the second or third trimester of uncomplicated pregnancy. C. trachomatis was investigated in cervical and urethral smears using direct immunofluorescence or Ligase chain reaction (LCR) for direct testing and immunoenzymatic assay (EIA) for serological testing and detection of specific IgG antibodies., Results: C. trachomatis was detected in 25.9% patients in group A, 35.8% in group B and 20% in group C and only in 12.7% in group D. IgG specific antibodies were present in 31.5%, 41.9%, 26.6% and 14.5% of patients in these groups respectively. The highest prevalence of chlamydial infections, regardless the diagnostic method used, was registered in the group of women with a history of 3 abortions (42.3% when direct testing and 45.5% when serological testing) while the lowest prevalence was in women who experienced only l abortion (23.2% and 28%)., Conclusion: C. trachomatis infection in pregnancy affects its duration as it can lead to miscarriage, death of the foetus and preterm delivery.

Based of literature data, the authors present informations on epidemiology, pathogenesis, clinical picture, diagnostics and treatment of Chlamydia trachomatis urogenital infections in children.

The antiphospholipid syndrome is considered to be one of the most common autoimmunological diseases. This paper is a review of contemporary literature data concerning pathogenesis and clinical and laboratory diagnostic criteria. Wide clinical symptomatology of the syndrome, differential diagnosis as well as diagnostic, therapeutic and preventive regimens are presented.

Based on a literature review, the authors present data on the epidemiology, pathogenesis, clinical picture, diagnostics and treatment of Chlamydia trachomatis urogenital infections and its complications in males.

Bacterial vaginosis (BV) is one of the most common lower genital tract infections among women of childbearing age. This paper is a survey of literature data concerning epidemiology, pathogenesis, clinical and laboratory diagnostic criteria of this clinical syndrome.

Introduction: The aim of the study was to evaluate the prevalence of anti-Chlamydia trachomatis (C.trachomatis) antibodies in serum and expressed prostatic secretion (EPS) in chronic prostatitis., Materials and Methods: Thirty-six patients with chronic prostatitis were examined. The presence of C. trachomatis was determined in urethral smears and EPS. Specific antibodies were determined in the serum (IgM, IgA, IgG) and in the EPS (IgA, IgG). In the direct diagnosis of chlamydial infection, the direct immunofluorescence method and the ligase chain reaction were employed, and for the serological diagnosis, the immunoenzymatic method., Results: C. trachomatis infection was detected in the urethra of 3 (8.3%) patients and in the prostatic gland of 3 (8.3%) patients; only one of these patients was found to have C. trachomatis in both the urethra and the EPS. In the control group, C. trachomatis was detected in the urethra of 1/50 (2%) of the men, but the EPS of all of them was free of C. trachomatis. Specific IgM antibodies were found in 7 (19.4%), IgA in 9 (25%), and IgG in 18 (50%) of the patients' serum, whereas IgAs were detected in 12 (33.3%) and IgGs in 13 (36.1%) of the patients' EPS. In the control group, anti-C. trachomatis antibodies of the IgG were detected in the serum of 2/35 (5.7%) of the men, whereas in the EPS neither IgA nor IgG antibodies were detected in any of these patients., Conclusions: Serological tests of the serum and EPS are useful as a complementary method in the diagnosis of chronic prostatitis.

Based on literature data, the authors have presented the informations on epidemiology, pathogenesis, clinical picture, diagnostics and treatment of Chlamydia trachomatis urogenital infections in men.

Chlamydia trachomatis (C. trachomatis) is one of the most common sexually transmitted bacterial agents. What distinguishes it from other organisms is its intracellular reproductive cycle. Up to now, four antigens have been identified in the Chlamydia genus: genus-specific antigen as well as species-specific, type-specific and subspecies-specific. C. trachomatis is a powerful immunogen which stimulates the host's immunological processes. The intracellular parasitism of the bacteria is the basis for both symptomatic or asymptomatic infection as well as for chronic ones. The primary infection leads to a local inflammatory reaction due to penetration and reproduction of the bacteria in the epithelial cells and to IgA secretory antibody production. In most cases the host's reaction to the primary infection is transient and does not cause tissue damage. In the course of chronic infection or reinfection, the most important processes are those of delayed hypersensitivity, which lead to a fast and intense immunological reaction of specifically sensitized Th1 lymphocytes. This reaction leads to progressive damage of the epithelial cells and to cicatrization and fibrosis, which means irreversible complications. Interferon gamma is of special importance in the process of C. trachomatis infection. High concentrations of it inhibit the bacteria's reproductive cycle, while lower concentrations promote the development of atypical, non-contagious forms of Chlamydia of diminished metabolic activity and altered antigenicity. The chlamydial heat shock proteins are considered to be of great importance lately. Their molecular weights of 60 and 10 kDa are a powerful stimulant of immunological reactions and show significant homology (40-90%) to human and other bacterial heat shock proteins.

The aim of the research was to determine the prevalence of C. trachomatis (C.t.) conjunctivitis and urogenital infections coexistence in adults. The study was carried out on 222 patients (109 women and 113 men) with clinical signs of conjunctivitis. Conjunctival swabs were taken from all patients for evaluation and urogenital swabs as well as blood from the patients with confirmed infection. Direct diagnostics of chlamydial infections involved IF and LCR methods while serologic diagnostics (specific IgG antibodies) used EIA and indirect IF methods. The control group for conjunctival swabs included 52 volunteers who did not have any ophthalmological symptoms while for the serological tests the control group consisted of 103 blood donors. Ocular C.t. infection was diagnosed in 42 out of 222 (18.9%) patients with conjunctivitis symptoms and in 2 out of 52 (3.8%) patients of the control group. Chlamydial conjunctivitis was mostly (70%) found in young people (18-30 yrs) and it was twice more common in women. It was usually bilateral and chronic. Urogenital C.t. infection was diagnosed in 24 out of 42 (57.1%) patients and it was more common in men (14 patients) than in women (10 patients). Only 4 out of 42 (9.5%) patients suffered from any urogenital symptoms. Specific IgG antibodies were found in the serum of 29 out of 42 (69%) patients with chlamydial conjunctivitis and only in 6 out of 103 (5.8%) patients of the control group. The diagnostics of the ocular infections, which are resistant to routine treatment, should involve the consideration of etiological role of C.t. Due to common urogenital chlamydial infection coexistence both sexual partners should be diagnosed and treated.

The aim of the research was to analyse the complaints and symptoms in acute Chlamydia trachomatis (C.t.) epididymitis. The analysis of data from history and physical examination was made in dependence on detected Chlamydia infection in the group of 39 patients with the symptoms of acute epididymitis (1--12 men C.t. (+), 2--27 men C.t.(-)). Chlamydial epididymitis more commonly occurred in younger patients, and the symptoms of the disease persisted for a longer time than in the patients with epididymitis of another etiology. None of the men had suffered from nongonorrhoeal epididymitis before, while two of them (16.7 percent) had had urethritis. The difference in etiology was also reflected in the patients' complaints. The discharge from the urethra was more common in the patients with Chlamydia infection (1--58.3 percent, 2--18.5 percent) while epididymis oedema and scrotum erythema where twofold scarcer. No exact correlation between C.t. presence and leukocyte reaction intensity in urethra was noticed. The occurrence of chlamydial epididymitis is not always preceded by symptoms of urethritis and only in some cases they are accompanied by the increase of polynuclear leukocytes in urethral discharge. Chlamydial epididymitis is of milder course when compared to epididymitis of another etiology.

The aim of the study was to evaluate the role of Chlamydia trachomatis (C.t.) in the etiopathogenesis of epididymitis. 39 patients with symptoms of acute epididymitis were examined. They were divided into two age groups: 1st--patients aged below 35 (24 patients) and 2nd--patients older than 35 yrs (15 patients). The patients' urethral swabs were examined and C.t., mycoplasma and other Gram-positive and Gram-negative bacteria were searched for as well as leukocyte count was assessed. Anti-Chlamydia trachomatis antibodies of IgG and IgM classes were assayed in serum. Direct immunofluorescence (antigen detection) and immunoenzymatic (antibodies detection) methods were used in C.t. infection diagnostics. C.t. infection in urethra was found in 30.8 percent of patients suffering from epididymitis, whereas in the control group in 2 percent. C.t. infection was significantly more commonly found in patients below 35 (45.8 percent) as compared with the older men (6.7 percent). The most common etiological factor in the younger group was C.t., while in the older group it was E. coli. IgG anti-C.t. antibodies were detected in the serum of 51.3 percent of the patients with acute epididymitis, as compared with 5.8 percent of the men in the control group. Specific IgG antibodies were found significantly more often in patients under 35 (66.7 percent) than in the older men (26.7 percent). Specific IgM antibodies were found in 15.4 percent of the patients, that is in 20.8 percent of the younger patients and in 6.7 percent of the older ones. C.t. is the main etiologic agent of epididymitis in men under 35.

The aim of our study was to determine the incidence of Chlamydia trachomatis (C. trachomatis) infections and its role in the etiopathogenesis of urinary tract infections in women. We studied 68 women, who where divided into two groups (I-with urethral syndrome, II-with other urologic diseases). With the use of direct diagnostic methods (immunofluorescence method, tissue culture) C. trachomatis infection was found in 25/68 (36.8%) women. In the group of women with urethral syndrome the percentage of positive results was higher in comparison to the second group, 53.8% and 26.2%, respectively. Specific antichlamydial antibodies of IgG class using direct immunoenzymatic method were found in 10/26 (38.5%) women with urethral syndrome. In the first group the infection was found more often in the urethra (urethra-85.7%, uterine cervix-50%) as in comparison to the second group. In the second group infection was found more often in the uterine cervix (urethra-45.5%, uterine cervix-72.7%). The incidence of C. trachomatis infection in women with urethral syndrome was in direct correlation with a larger number of polynuclear leukocytes in urine sediment (more than 3 in the viewing field). C. trachomatis infection was found in 76.5% of women with leukocyturia and 12.5% of women in which the number of leukocytes in urine sediment was less than 3 in the viewing field. The incidence of Ureaplasma urealyticum and Mycoplasma hominis infections between both study groups did not differ significantly. However, the incidence of yeast-like fungi infections in the group of women with other urologic diseases was double. The clinical sign most often found in women infected with C. trachomatis and with urethral syndrome was dysuria, which was present in all 14 women. In the group of women with other urologic diseases the clinical sign found most often was pollakisuria (72.7%).

Investigation included 254 women (18-75 years of age). In this number, 74 patients constituted a group with pathological changes in cervix uteri, while 180 women were seemingly healthy. The material was evaluated by a method of direct immunofluorescence (IF) with monoclonal antibody serum and a culture on McCoy cells. Antibodies in blood serum was detected by an immunoenzymatic test ELISA. Out of total number of 256 patients tested, elementary bodies of C. trachomatis were found in cylindrical cells of cervix uteri by the IF test in 54 (21.25%) of women. Analogical percentage of positive results was obtained by application of culture on McCoy cells. Positive serological results were found in 70 patients (27.55%). In group of 75 patients infected with C. trachomatis, 33 cases (44.59%) were confirmed by application of IF and cell culture, whereas positive serological results were found in 39 (52.70%) of investigated patients. In the group of seemingly healthy women in 11.60% elementary bodies of C. trachomatis were detected and in 17.22% of cases serological results were positive.