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In this paper, the Stellaria media plant, which is part of the Caryophyllaceae family, is highlighted from the point of view of its chemical composition through qualitative and quantitative methods such as Folin-Ciocalteu and HPLC-DAD_ESI for highlighting the total phenols. The biomedical properties were also tested, such as the antioxidant capacity through the DPPH and TEAC methods, the antimicrobial capacity compared to different antibiotics on E. coli and S. aureus, but also the cicatrizing capacity in vitro on normal human dermal fibroblast-type cells of the plant extract under two forms of application (extract 1 mg/ml respectively its liposomal form) compared to the positive control (liposomes without extract included and 50 mg/ml alanine) and negative control (untreated in vitro simulated wound). To be able to apply the liposomal formula with included extract on NHDF cells, liposomes were formulated using cholesterol and phosphatidylserine in a ratio of 2:3 by the lipid film hydration method (with phosphate buffer pH 7.6). The liposomal formula was characterized by microscopy, DLS analysis, and Extract Inclusion Efficiency (EE%). Last but not least, the samples applied to highlight the cicatrizing effect were tested by the MTS method regarding their toxicity on fibroblast cells. [ABSTRACT FROM AUTHOR]