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Sap flow represents water transport from roots to leaves through the xylem and is used to describe tree transpiration. This paper proposed and tested a procedure to estimate sap flow by calculating global radiation in a digital model of the tree canopy surface obtained by unmanned aerial vehicle imaging. The sap flow of nine trees was continuously measured in the field. In the digital surface model, individual canopies were automatically delineated, their parameters were determined and the global radiation incident on their surface on specific days was calculated. A polynomial relationship was found between sap flow and the calculated incident solar radiation during the morning hours with a coefficient of determination of 0.98, as well as a linear relationship between the decrease in radiation and sap flow during the afternoon with a correlation coefficient of 0.99. Using the Random Forest machine learning method, a model predicting the sap flow of the trees was created based on the global radiation and canopy parameters determined from the digital surface model of tree canopies. The resulting model was deployed on additional days and compared to field measurements of sap flow, achieving a correlation coefficient of 0.918. In addition, two linear regression models were created for a tree group, achieving coefficients of determination of 0.66 and 0.90.

Teak has been planted extensively by smallholders in Central America within reforestation programmes and has become one of the most valuable timbers. The five-year growth and mortality of teak cultivated in a plantation in central Nicaragua, representing sites at the low limit of teak ecological valence, were evaluated. From 2006 to 2010, 72 pure teak stands were established, with 48.93 ha in total. For afforestation, planting stock from five provenances was used and planted at 1 m × 1 m spacing to stimulate the height growth and reduce broad crowns formed by self-pruning. In the pure teak stands, 144 permanent sample plots of 0.01 ha in size were established in 2011. From 2011 until 2015, the tree height and stem girth of all individuals in the studied sample plots were measured, and mortality based on the stand density was assessed. Significant differences between the provenances were observed. The highest growth was noted in the Local provenance originated from the studied area, whereas the Semilla provenance from Costa Rica, characterised by the lowest growth ability, was characterized by lower radial increment and mean tree height with high mortality.

Agriculture and forestry have traditionally been one of the most hazardous occupations for workers. In both these sectors the tractor is one of the most used machinery. From a total of 89 detected serious and fatal accidents with tractors in the Czech Republic between the years 2009 and 2018 were 72 serious and 17 fatal. All the accidents affected men (no woman was affected). Men around 56 with low practice length were most at risk of injury. Categories created by the State Labour Inspection Office of the Czech Republic assign exactly one category to each injury. The most common cause of the accident was poor or insufficiently estimated risk (in 62 of 89 cases). Own accident categories were created, and more than one category of cause was assigned to one injury if found. The most common cause of the accident was an incorrect procedure and breach of rules. The analysis of accidents and related information revealed that out of 89 cases the injury became most often to a tractor operator (in 47 cases) and outside the cab (in 50 cases). Within the labour relations, 14% of the injuries were fatal and 86% were serious; outside of labour relations, 67% of the injuries were fatal and 13% were serious.

The leaf area index (LAI) is one of the most common leaf area and canopy structure quantifiers. Direct LAI measurement and determination of canopy characteristics in larger areas is unrealistic due to the large number of measurements required to create the distribution model. This study compares the regression models for the ALS-based calculation of LAI, where the effective leaf area index (eLAI) determined by optical methods and the LAI determined by the direct destructive method and developed by allometric equations were used as response variables. LiDAR metrics and the laser penetration index (LPI) were used as predictor variables. The regression models of LPI and eLAI dependency and the LiDAR metrics and eLAI dependency showed coefficients of determination (R2) of 0.75 and 0.92, respectively; the advantage of using LiDAR metrics for more accurate modelling is demonstrated. The model for true LAI estimation reached a R2 of 0.88.

The objective of the present paper is to confirm or reject the possible use of recycled asphalt to reinforce forest haul roads regarding the technical requirements set by the standards and directives relevant to the construction of forest road surfaces. The hypothesis is based on the presumption that recycled materials, if correctly used, can reach the same construction properties as standard materials, hence their application does not have a negative effect on reinforcement quality. On a selected stretch of the forest road, three test sections were constructed with the use of recycled asphalt, however, each of them with a different technological solution. The first section was reinforced with unbound mixture – Type1 without added water, the second section was constructed using a version of vibrated macadam technology, and recycled asphalt was applied to the third section by the method of basic compacting. In each of the sections, tacheometric cross profile measurement was carried out at monthly intervals to monitor the changes in the cross profile shape, and the number of passages of fully loaded logging trucks was registered; static load tests were performed at pre-defined time intervals to determine the deformation moduli such as deformation characteristics of the road surface structural layers. In all three reinforcement versions, the values of deformation moduli observed during the static load tests were between 68–90% of the values set by relevant standards for these technologies using natural aggregates. However, the tacheometric measurements did not reveal statistically significant changes in the shape of the reinforcement cross-section. Based on the obtained results, applying recycled asphalt to reinforce forest roads seems to be a suitable alternative to natural quarry aggregate used in unbound structural layers. Recycled material needs to meet the regulatory limits for foreign elements and pass ecotoxicity tests, which is evidenced by a certificate on material compliance issued by the test laboratory.

Forests make up 34.1% of the Czech Republic total area and forest roads account for nearly the same length (47,465 km) as all other roads administered by the state and its regions (55,738 km). Forest roads are not as intensively used as other roads. On the other hand, as logging trucks carry the maximum permitted load on roads and forests create a specific microclimate, forest roads are subject to rapid wear. A road wearing course is generally designed for 20 years of service and for a maximum damage level of 25% before they are supposed to be reconstructed. To ensure this life cycle is adhered to, more efficient, faster, and more flexible surface damage detection adaptable for forest environment is needed. As smartphones and their optical devices, i.e., new iPhones with LiDAR sensors, become more advanced, the option arises to perform laser scanning on road surfaces using smartphones applications. This work aimed to test this technology and its precision applicability to assessing damage to a forest wearing course and compare it with another hand-held personal laser scanner (PLShh), represented in this study by GeoSLAM ZEB Horizon scanner, and more precise terrestrial laser scanning (TLS) technology, represented in this study by Faro Focus 3D laser scanner, which have started to replace tacheometric wearing course damage surveying thanks to their greater precision. So, this study involved a comparison of three alternative laser scanning methods focused especially on these, which are implemented in new iPhones for tacheometric surveying. First, a Faro Focus 3D laser scanner was used for the TLS method. Second, the PLShh method was tested on a GeoSLAM ZEB Horizon scanner. Third, another PLShh method using an iPhone 13 Pro with applications 3D Scanner and Polycam was evaluated. If we are comparing positional height accuracy of PLShh to tacheometric surveying on reference cross position height coordinates, ZEB Horizon achieved devXY and devZ RMSE 0.108 m; 0.025 m; iPhone 13 Pro with 3D Scanner app devXY and devZ RMSE 0.185 m; 0.021 m, and with Polycam app devXY and devZ RMSE 0.31 m; 0.045. TLS achieved the best results with devXY RMSE 0.049 and devZ RMSE 0.0077. The results confirm that only the TLS scanner achieves precision values in height differences applicable for an assessment of forest road wearing course damage measurement comparable with tacheometric surveying. Surprisingly, comparing the PLShh scanners to the TLS technology, they achieved interesting results, comparing their transverse profiles and 3D objects as digital surface models (DSM) of the road to TLS in height position. In transverse profiles, ZEB Horizon achieved devZ RMSE 0.032 m; iPhone 13 Pro with 3D Scanner app devZ RMSE 0.017 m, and with Polycam app devZ RMSE 0.041 m compared to the TLS method measured using a Faro Focus 3D static laser scanner. Comparing forest road DSM to Faro Focus 3D, ZEB Horizon achieved devZ RMSE 0.028 m; iPhone 13 Pro with 3D Scanner app devZ RMSE 0.018 m and with Polycam devZ RMSE 0.041 m. These results in height differences show that the height accuracy of PLShh achieves precision, which is applicable to determining the current shape of forest road wearing course compared to the required roof shape gradient. However, further testing provided the insight that such a kind of PLShh measurement is still only possible to use for the identification of a transverse profile shape, as in length measurement the length error increases. All PLShh are able to capture the current shape of forest road cross profile, but still they cannot be used for any design or calculation of material measurement needed for wearing course repair.

The article is on the effects of woody vegetation growing on the roadside on the temperature of the surface of cycle paths. The main hypothesis of the study is that vegetation has the effect of lowering the temperature of the surroundings in its shadow and thus improves the comfort of users of cycle paths in the summer months. The second hypothesis is to find out which type of road surface is most suitable for the thermal well-being of users. This goal was achieved by measuring the temperature of selected locations on cycle paths with different types of construction surfaces with nearby woody vegetation using a contactless thermometer over several days at regular intervals. The positions of the selected locations were measured using GNSS and the whole locality of interest was photographed using an unmanned aerial vehicle (UAV), or drone, and subsequently a digital surface model (DSM) of the area was created using a Structure from Motion (SfM) algorithm. This model served for the calculation of incident solar radiation during the selected days using the Solar Area Graphics tool with ArcGIS software. Subsequently, the effect of the shade of the surrounding vegetation on the temperature during the day was analysed and statistically evaluated. The results are presented in many graphs and their interpretation used to evaluate the effects of nearby woody vegetation and the type of road surface on the surrounding air temperature and the comfort of users of these routes. The results demonstrate the benefits of using UAVs for the purpose of modelling the course of solar radiation during the day, showing the effect of roadside vegetation on reducing the surface temperature of the earth’s surface and thus confirming the need for planting and maintaining such vegetation.

Over the last decades, climate change has triggered an increase in the frequency of spruce bark beetle (Ips typographus L.) in Central Europe. More than 50% of forests in the Czech Republic are seriously threatened by this pest, leading to high ecological and economic losses. The exponential increase of bark beetle infestation hinders the implementation of costly field campaigns to prevent and mitigate its effects. Remote sensing may help to overcome such limitations as it provides frequent and spatially continuous data on vegetation condition. Using Sentinel-2 images as main input, two models have been developed to test the ability of this data source to map bark beetle damage and severity. All models were based on a change detection approach, and required the generation of previous forest mask and dominant species maps. The first damage mapping model was developed for 2019 and 2020, and it was based on bi-temporal regressions in spruce areas to estimate forest vitality and bark beetle damage. A second model was developed for 2020 considering all forest area, but excluding clear-cuts and completely dead areas, in order to map only changes in stands dominated by alive trees. The three products were validated with in situ data. All the maps showed high accuracies (acc > 0.80). Accuracy was higher than 0.95 and F1-score was higher than 0.88 for areas with high severity, with omission errors under 0.09 in all cases. This confirmed the ability of all the models to detect bark beetle attack at the last phases. Areas with no damage or low severity showed more complex results. The no damage category yielded greater commission errors and relative bias (CEs = 0.30–0.42, relB = 0.42–0.51). The similar results obtained for 2020 leaving out clear-cuts and dead trees proved that the proposed methods could be used to help forest managers fight bark beetle pests. These biotic damage products based on Sentinel-2 can be set up for any location to derive regular forest vitality maps and inform of early damage.

This research was focused on the study of visual exposure evolution in the locality of the Drátenická skála nature monument (in the Czech Republic) and the surrounding forest complex in terms of history and through modelling for further possible stand development. The local forests underwent conversion from a natural fir-beech composition to an intensive spruce monoculture with few insect pests or windbreak events to an actual bark beetle infestation. Historic maps, landscape paintings, photographs, and orthophotos served as the basic materials for the illustration of the past situation. Further development was modelled using canopy height models and spectral properties captured by unmanned aerial vehicles (UAVs). As an example, the possible situation of total mortality among coniferous spruce trees after a bark beetle outbreak was modelled. Other options and a practical use of such preprocessed data are, for example, a model for opening and transforming the stands around the rock as one of the ongoing outcrop management trends in the protected landscape area (PLA) of Žďárské vrchy.

Populations of Dracaena serrulata are disappearing at an alarming rate in the Arabian Peninsula. They are being destroyed by herders who use the leaves as fodder for camels, goats, and sheep during the dry season. Up until now, precise information about the current distribution and population status of D. serrulata in Oman have not been published. To fill this gap, the main aim of this work was to map the species distribution in the Dhofar Mountains (Oman) and to define the conservation and health status of the populations. Three isolated sub-populations of the study species were defined and mapped: the Jabal Samhan, Jabal al Qara, and Jabal al Qamar sub-populations. Dracaena serrulata occupies an area of 227 km2 in the Dhofar Mountains. More than 43,000 trees were counted, and 2387 trees were inventoried in total. The Jabal Samhan sub-population is an example of an extensively damaged population with 59% of the trees recorded as dead and only 21% healthy trees. Populationsin the western portions of the Dhofar Mountains., Jabal al Qamar, and Jabal al Qara are comparatively abundant stands of healthy trees with a higher proportion of seedlings. The health of trees is strongly influenced by accessibility and precipitation provided by the southwest summer monsoon: the healthy individuals predominate on the steep terrain along the seaward facing cliffs.

This paper reviews the use of modern 3D image-based and Light Detection and Ranging (LiDAR) methods of surface reconstruction techniques for high fidelity surveys of small rock outcrops to highlight their potential within structural geology and landscape protection. LiDAR and Structure from Motion (SfM) software provide useful opportunities for rock outcrops mapping and 3D model creation. The accuracy of these surface reconstructions is crucial for quantitative structural analysis. However, these technologies require either a costly data acquisition device (Terrestrial LiDAR) or specialized image processing software (SfM). Recent developments in augmented reality and smartphone technologies, such as increased processing capacity and higher resolution of cameras, may offer a simple and inexpensive alternative for 3D surface reconstruction. Therefore, the aim of the paper is to show the possibilities of using smartphone applications for model creation and to determine their accuracy for rock outcrop mapping.

Detailed, three-dimensional modeling of trees is a new approach in botanical taxonomy. Representations of individual trees are a prerequisite for accurate assessments of tree growth and morphological metronomy. This study tests the abilities of 3D modeling of trees to determine the various metrics of growth habit and compare morphological differences. The study included four species of the genus Dracaena: D. draco, D. cinnabari, D. ombet, and D. serrulata. Forty-nine 3D tree point clouds were created, and their morphological metrics were derived and compared. Our results indicate the possible application of 3D tree point clouds to dendrological taxonomy. Basic metrics of growth habit and coefficients derived from the 3D point clouds developed in the present study enable the statistical evaluation of differences among dragon tree species.

Currently, a large part of forest roads with a bituminous surface course constructed in the Czech Republic in the second half of the last century has been worn out. The aim of the study is to verify the possibility and the accuracy of the road wearing course damage detected by four different remote sensing methods: close range photogrammetry, terrestrial laser scanning, mobile laser scanning and airborne laser scanning. At the beginning of verification, cross sections of the road surface were surveyed geodetically and then compared with the cross sections created in the DTMs which were acquired using the four methods mentioned above. The differences calculated between particular models and geodetic measurements show that close range photogrammetry achieved an RMSE of 0.0110 m and the RMSE of terrestrial laser scanning was 0.0243 m. Based on these results, we can conclude that these two methods are sufficient for the monitoring of the asphalt wearing course of forest roads. These methods allow precise and objective localization, size and quantification of the road damage. By contrast, mobile laser scanning with an RMSE of 0.3167 m does not reach the required precision for the damage detection of forest roads due to the vegetation that affects the precision of the measurements. Similar results are achieved by airborne laser scanning, with an RMSE of 0.1392 m. As regards the time needed, close range photogrammetry appears to be the most appropriate method for damage detection of forest roads.

Abstract Objectives Resistance to antibiotics among bacteria of clinical importance, including Staphylococcus aureus, is a serious problem worldwide and the search for alternatives is needed. Some metal complexes have antibacterial properties and when combined with antibiotics, they may increase bacterial sensitivity to antimicrobials. In this study, we synthesized the iron complex and tested it in combination with ampicillin (Fe16 + AMP) against S. aureus. Methods An iron complex (Fe16) was synthesized and characterized using spectroscopy methods. Confirmation of the synergistic effect between the iron complex (Fe16) and ampicillin (AMP) was performed using ζ–potential, infrared spectra and FICI index calculated from the minimum inhibitory concentration (MIC) from the checkerboard assay. Cytotoxic properties of combination Fe16 + AMP was evaluated on eukaryotic cell line. Impact of combination Fe16 + AMP on chosen genes of S. aureus were performed by Quantitative Real-Time PCR. Results The MIC of Fe16 + AMP was significantly lower than that of AMP and Fe16 alone. Furthermore, the infrared spectroscopy revealed the change in the ζ–potential of Fe16 + AMP. We demonstrated the ability of Fe16 + AMP to disrupt the bacterial membrane of S. aureus and that likely allowed for better absorption of AMP. In addition, the change in gene expression of bacterial efflux pumps at the sub-inhibitory concentration of AMP suggests an insufficient import of iron into the bacterial cell. At the same time, Fe16 + AMP did not have any cytotoxic effects on keratinocytes. Conclusions Combined Fe16 + AMP therapy demonstrated significant synergistic and antimicrobial effects against S. aureus. This study supports the potential of combination therapy and further research.