Search

Abstract: Four solid-phase reagents have been tested for indirect determination of cyanide using flow injection analysis-flame atomic absorption spectrometry (FIA-FAAS). The method is based on insertion of aqueous cyanide solutions into an on-line Ag2X (where X are SO3 2−, Cr2O7 2−, C2O4 2− and CO3 2−) packed column (25%, m/m suspended on silica gel beads) and re-distilled water or sodium hydroxide are used as the carrier stream. The eluent containing the analyte as silver cyanide complexes, produced from reaction between Ag2X and cyanide, measured by flame atomic absorption spectrometry. The method is simple, fast and selective than other published FIA procedures. A relative standard deviation (R.S.D.) better than 1.12% was obtained in a repeatability study. The method was applied to the determination of cyanide in industrial electrolytic baths. [Copyright &y& Elsevier]

The porous materials known as metal-organic frameworks (MOFs) stand out for their enormous surface area, adaptable pore size and shape, and structural variety. These characteristics make them well-suited for various applications, especially in healthcare. This review thoroughly summarizes recent studies on the use of MOFs in drug delivery, biosensing, and therapeutics. MOFs may encapsulate medications, target certain cells or tissues, and regulate their release over time. Additionally, MOFs have the potential to be used in biosensing applications, allowing for the selective detection of chemical and biological substances. MOFs' optical or electrical characteristics may be modified to make biosensors that track physiological data. MOFs show potential for targeted drug delivery and the regulated release of therapeutic substances in cancer treatment. In addition, they may work as potent antibacterial agents, providing a less dangerous option than traditional antibiotics that increase antibiotic resistance. For practical applications, further research is required as well as more consideration for the problems with toxicity and biocompatibility. In addition to addressing the difficulties and promising possibilities in this area, this study intends to provide insights into the potential of MOFs in healthcare for drug delivery, biosensing, and treatment. Despite several essential reviews in this area, it was necessary to look into the most recent research on drug delivery, biosensing, and therapy as a combined concept.

Wearable sweat sensors are reshaping healthcare monitoring, providing real-time data on hydration and electrolyte levels with user-friendly, noninvasive devices. This paper introduces a highly portable two-channel microfluidic device for simultaneous sweat sampling and the real-time detection of volatile organic compound (VOC) biomarkers. This innovative wearable microfluidic system is tailored for monitoring diabetes through the continuous and noninvasive tracking of acetone and ammonia VOCs, and it seamlessly integrates with smartphones for easy data management. The core of this system lies in the utilization of carbon polymer dots (CPDs) and carbon dots (CDs) derived from monomers such as catechol, resorcinol, o -phenylenediamine, urea, and citric acid. These dots are seamlessly integrated into hydrogels made from gelatin and poly(vinyl alcohol), resulting in an advanced solid-state fluorometric sensor coating on a cellulose paper substrate. These sensors exhibit exceptional performance, offering linear detection ranges of 0.05-0.15 ppm for acetone and 0.25-0.37 ppm for ammonia, with notably low detection limits of 0.01 and 0.08 ppm, respectively. Rigorous optimization of operational parameters, encompassing the temperature, sample volume, and assay time, has been undertaken to maximize device performance. Furthermore, these sensors demonstrate impressive selectivity, effectively discerning between biologically similar substances and other potential compounds commonly present in sweat. As this field matures, the prospect of cost-effective, continuous, personalized health monitoring through wearable VOC sensors holds significant potential for overcoming barriers to comprehensive medical care in underserved regions. This highlights the transformative capacity of wearable VOC sweat sensing in ensuring equitable access to advanced healthcare diagnostics, particularly in remote or geographically isolated areas.

Monitoring the levels of L-Tryptophan (L-Trp) in body fluids is crucial due to its significant role in metabolism and protein synthesis, which ultimately affects neurological health. Herein, we have developed a novel magneto-responsive electrochemical enantioselective sensor for the recognition of L-Trp based on oriented biochar derived from Loofah, Fe 3 O 4 nanoparticles, and molecularly imprinted polydopamine (MIPDA) in xanthan hydrogel. The successful synthesis of these materials has been confirmed through physicochemical and electrochemical characterization. Various operational factors such as pH, response time, loading sample volume, and loading of active materials were optimized. As a result, the sensor exhibited an affordable linear range of 1.0-60.0 μM, with a desirable limit of detection of 0.44 μM. Furthermore, the proposed electrochemical sensor demonstrated good reproducibility and desirable selectivity for the determination of L-Trp, making it suitable for analyzing L-Trp levels in human plasma and serum samples. The development presented offers an appealing, easily accessible, and efficient strategy. It utilizes xanthan hydrogel to improve mass transfer and adhesion, biochar-stabilized Fe 3 O 4 to facilitate magnetic orientation and accelerate mass transfer and sensitivity, and polydopamine MIP to enhance selectivity. This approach enables on-site evaluation of L-Trp levels, which holds significant value for healthcare monitoring and early detection of related conditions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Septicemia, a severe bacterial infection, poses significant risks to human health. Early detection of septicemia by tracking specific biomarkers is crucial for a timely intervention. Herein, we developed a molecularly imprinted (MI) TiO 2 -Fe-CeO 2 nanozyme array derived from Ce[Fe(CN) 6 ] Prussian blue analogues (PBA), specifically targeting valine, leucine, and isoleucine, as potential indicators of septicemia. The synthesized nanozyme arrays were thoroughly characterized using various analytical techniques, including Fourier transform infrared spectroscopy, X-ray diffraction, field-emission scanning electron microscope, and energy-dispersive X-ray. The results confirmed their desirable physical and chemical properties, indicating their suitability for the oxidation of 3,3',5,5'-tetramethylbenzidine serving as a colorimetric probe in the presence of a persulfate oxidizing agent, further highlighting the potential of these arrays for sensitive and accurate detection applications. The MITiO 2 shell selectively captures valine, leucine, and isoleucine, partially blocking the cavities for substrate access and thereby hindering the catalyzed TMB chromogenic reaction. The nanozyme array demonstrated excellent performance with linear detection ranges of 5 μM to 1 mM, 10-450 μM, and 10-450 μM for valine, leucine, and isoleucine, respectively. Notably, the corresponding limit of detection values were 0.69, 1.46, and 2.76 μM, respectively. The colorimetric assay exhibited outstanding selectivity, reproducibility, and performance in the detection of analytes in blood samples, including C-reactive protein at a concentration of 61 mg/L, procalcitonin at 870 ng/dL, and the presence of Pseudomonas aeruginosa bacteria. The utilization of Ce[Fe(CN) 6 ]-derived MITiO 2 -Fe-CeO 2 nanozyme arrays holds considerable potential in the field of septicemia detection. This approach offers a sensitive and specific method for early diagnosis and intervention, thereby contributing to improved patient outcomes.

Quantifying creatinine (Cn) in biological fluids is crucial for clinically assessing renal insufficiency, thyroid irregularities, and muscle damage. Therefore, it is crucial for human health to have a simple, quick, and accurate Cn analysis technique. In this study, we have successfully synthesized a 3D ratiometric dual-metal-organic framework, namely, the amino-MIL-53@Mo/ZIF-8 and rhodamie B heterostructure, using an internal strategy for sustained growth. The dual-MOF functions as an adsorbent and preconcentrates Cn. The pH, reaction time, and volume ratio of amino-MIL-53@Mo/ZIF-8/rhodamie B were optimized using the one-variable-at-a-time technique in this study. The quantitative study of the Cn concentration for this RF biosensor was obtained under ideal conditions ( R 2 = 0.9962, n = 3), encompassing the linear range of 0.35-11.1 μM. The detection and quantitation limits were 0.18 and 0.54 nM, respectively. Both intra- and interday reproducibility showed high repeatability of the RF biosensor, UV-vis, and ZETA potential studies, and the Stern-Volmer relationship was used to clarify the fluorescence quenching process. These superior sensing capabilities and the benefits of simple manufacturing, acceptable stability, and practicality make the RF biosensor intriguing for ultrasensitive Cn detection in practical applications.

"The development and deployment of a practical and portable technology for on-site chiral identification of enantiomers hold immense significance in the fields of medical and biological sciences. Among the essential amino acids, Tryptophan (Trp) plays a crucial role in human metabolism and serves as a diagnostic marker for various metabolic disorders. In this study, we introduce an innovative approach that combines an enantio-selective ZIF-8-His MOF-MIPs packed-bed centrifugal microfluidic system with an enantioselective colorimetric sensor probe. This system is further integrated with smartphone-based on-site data recording. The basis of this colorimetric sensor's operation lies in the controlled morphology and surface passivation of gold nano-ovals (Au-NOs) through DL-Alanine. To confirm the successful synthesis of the chiral recognition elements, we employed various characterization techniques, including FE-SEM, TEM, FTIR, CD, UV-Vis, zeta potential, DLS, and XRD. Our focus was on optimizing operational parameters for the effective separation and determination of L-chiral tryptophan on-site. The sensor exhibited two linear ranges for L-Trp detection: 0-5.42 and 5.42-80.47 mM, with a detection limit of 0.5 mM. The integrated system possesses advantages such as ease of availability, preparation, high stability, desirable selectivity even in the presence of similar biomolecules, and rapid detection capabilities. Furthermore, our method demonstrated successful enantioselective sensing of L-Trp in various biological samples, including human blood plasma, urine, milk, and bovine serum albumin (BSA), yielding promising results. The integrated microfluidic platform follows a "sample-in and answer-out" approach, making it highly applicable in healthcare, environmental monitoring, food safety analysis, and point-of-care testing. The chiral recognition pretreatment assay and self-contained, automated colorimetric detection on the microfluidic disc represent a promising avenue for cutting-edge research in these domains"., Competing Interests: Declaration of competing interest There is no conflict of interest., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Herein, we introduce a novel integrated system that merges an enantio-discriminative bio-MOF-packed centrifugal microfluidic chip made from PDMS with a user-friendly on-site colorimetric sensor. This innovative approach enables the precise enantioselective recognition of L-tryptophane (L-Trp). This chiral recognition probe was successfully synthesized through meticulous control of nano-ovals-shaped gold nanoparticles morphology and surface passivation. The operational factor of this methodology was optimized to ensure simplicity, practicality, and efficiency. This optimization led to reduced reagent consumption and instantaneous analytical feedback. The integrated system was effectively applied for enantioselective separation and quantification of L-Trp across an extensive linear range of 50 μM-1.5 mM, impressive limit of detection as low as 15 μM. It is noteworthy that this integrated system demonstrated desirable selectivity even in the presence of similar biomolecules, showcasing its robust performance and rapid detection capability. Further extended the application of this strategy to exceptional performance across enantioselective sensing of L-Trp in various sample matrices, comprising bovine serum albumin, bovine milk, blood plasma and urine samples. This integrated microfluidic sample pretreatment, chiroptical sensing, and on-site signal recording with a smartphone hold tremendous potential for widespread implementation, practical applications engaging healthcare and environmental, food safety, and point-of-needs analysis, facilitating successive solution mixing and colorimetric detection., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Wearable sweat sensors present exciting opportunities for advancing personal health monitoring and noninvasive biomarker measurements. However, existing sensors often fall short in accurate detection of low analyte volumes and concentrations and lack multimodal sensing capabilities. Herein, we present a highly portable four-channel microfluidic device capable of conducting simultaneous sweat sampling and fluorometric sensing of potential biomarkers, such as l-Tyr, l-Trp, Crt, and NH 4 + , specifically designed for kidney disease monitoring. Our microfluidic device seamlessly integrates with smartphones, facilitating easy data retrieval and analysis. The core of the sensing array is a novel fluorometric solid-state mechanism utilizing carbon polymer dots derived from dopamine, catechol, and o -phenylenediamine monomers embedded in gelatin hydrogels. The sensors exhibit exceptional performance, offering linear ranges of 5-275, 6-170, 4-220, and 5-170 μM, with impressively low detection limits of 1.5, 1.2, 1.3, and 1.4 μM for l-Tyr, l-Trp, Crt, and NH 4 + , respectively. Through meticulous optimization of operational variables, comprising the temperature, sample volume, and assay time, we achieved the best performance of the device. Furthermore, the sensors exhibited remarkable selectivity, effectively distinguishing between biologically similar species and other potential biological compounds found in sweat. Our evaluation also extended to monitoring kidney diseases in patients and healthy individuals, showcasing the device's utility in world scenarios. Promising results showcase the potential of low-cost, multidiagnostic microfluidic sensor arrays, especially with synthetic skin integration, for enhanced disease detection and healthcare outcomes.

Investigating effective fluorescence strategies for real-time monitoring of dipicolinic acid (DPA) is of paramount importance in safeguarding human health. Herein, we present the design of a desirable red-emissive carbon nanostructure anchoring a molecularly imprinted Er-BTC MOF as a fluorescence biosensor for the visual determination of DPA. DPA is a biomarker of Bacillus anthracis , a subcategory of serious infectious diseases and bioweapons. We introduce a paper test strip sensitized with the aforementioned nanostructure, which is integrated with online UV excitation and smartphone digital imaging, resulting in a DPA signal-off sensing platform. The proposed fluorometric visual paper-based biosensor demonstrates wide linear ranges for DPA (10-125 μM) with a LOQ and LOD of 4.32 and 1.28 μM, respectively. The designed platform exhibits impressive emission properties and adaptable surface functional groups, which confirm its desirable selective sensing capabilities against other biological molecules and DPA isomers. As a proof of concept, DPA monitoring is successfully applied to real samples of tap water and urine. This integrated selective paper-based nano-biosensor, coupled with smartphone signal recording, holds great promise for state-of-the-art practical applications including fluorometric/colorimetric detection in healthcare and environmental monitoring, food safety analysis, and point-of-care testing.

The advantages of porosity and stable unpaired electrons of porphyrinic organic polymers (POPs) with free radicals are exclusive and potentially practical functionalities and combining the semiconductor-like characteristics of these materials and metal ions has been an effective way to assemble an efficient photocatalytic system. Herein, a new ruthenium (Ru) ion-encapsulated porphyrinic organic polymer (POP/Ru) is facilely synthesized as a proper photoresponsive nanozyme with unique photo-oxidase properties. Surprisingly, the proposed POP/Ru revealed outstanding photoresponsive oxidase-mimicking activity due to the synergetic effect of the integration of Ru and π-electrons of POP, which boosts charge separation and transport. POP/Ru was applied to the oxidation of o -phenylenediamine ( o -PDA) as a chromogenic probe for producing a colorimetric signal. The kinetic study reveals that these photo-oxidase mimics have a significant affinity for the o -PDA chromogenic agent owing to a lower K m and superior V max . Further findings demonstrate that the presence of the l-arginine (l-Arg) target causes an inhibition effect on the photo-nanozymatic colorimetry of POP/Ru. This research develops the applications of the comprehensive colorimetric strategy for ultrasensitive l-Arg monitoring with a limit of detection (LOD) of 15.2 nM in the dynamic range of 4.0 nM-340 μM and illuminates that the proposed photo-oxidase nanozyme as a visual strategy is feasible in l-Arg environmentally friendly colorimetric detection in juice samples.

Kidney dysfunction is a clinical disease that disables the kidneys to remove the waste products and uremic toxins from the circulation and may lead to fatal kidney failure. Hemodialysis is advantageous in this circumstance since it prevents the accumulation of waste products in the body and facilitates the removal of uremic toxins. However, hemodialysis cannot entirely remove some uremic toxins, such as urea and creatinine. In this paper, a high-performance fixed-bed column for urea and creatinine removal was offered. As a result, a MOF layer was built on SiO 2 , which was then amino-functionalized using APTES. Numerous assays were used to characterize the final adsorbent. The adsorption of urea and creatinine was evaluated in batch and continuous conditions. Thus, it was demonstrated that the adsorption behavior of A(0.2)-IRMOF-1@SiO 2 followed the Langmuir isotherm, and it exhibited the maximum adsorption capacity. The batch experiment determined that urea and creatinine had an adsorption capacity of 1325.73 and 625.00 mg·g -1 , respectively. The adsorption capacity was increased, which was due to the presence of amino groups (APTES) on the MOF surface. The continuous operation was evaluated using the A(0.2)-IRMOF-1@SiO 2 fixed-bed column. Thomas and Nelson's models were examined to achieve a better understanding of the adsorption behaviors. The A(0.2)-IRMOF-1@SiO 2 fixed-bed column successfully removed 92.57% of urea and 80.47% of creatinine. The separation factor for urea in comparison to creatinine was 2.40 in the A(0.2)-IRMOF-1@SiO 2 fixed-bed column.

This study reports the development of a vanadium-docked porphyrinic covalent organic framework as a novel class of highly polar photoactive materials. Thanks to its extended π-electron conjugation and high chemical stabilities, this framework can serve as an oxidase-Like photo-nanozyme for photocatalytic oxidation of o-phenylenediamine (o-PDA) and a colorimetric substrate for the production of the yellow-colored oxidized o-PDA (o-PDAox). The physicochemical properties of the as-prepared photo-nanozyme were characterized by several analytical techniques. Its enhanced light harvesting and charge separation and transfer were also verified by electrochemical and spectroscopic analysis. This photo-nonenzymatic colorimetric assay was applied for the sensitive L-Arginine (L-Arg) detection as a typical amino acid in the linear range of 8.1 nM-330 μM with a limit of detection (LOD) of 3.5 nM. The findings of this research confirmed the safety and feasibility of the proposed photo-nonenzymatic colorimetric sensing strategy for the detection of L-Arg and other similar biomolecules in food samples. Kinetic investigation revealed that the photo-responsive oxidase mimic exhibits satisfactory K m (0.47 mM) and V max (42.0 μM/s) values. This work broadened our insight into the development of modified porphyrinic-COF-based visible light-responsive oxidase-like photo-nanozyme for environmentally friendly colorimetric biosensing., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Colorimetric sensing is a low-cost and visual method for food freshness monitoring. We prepared a Vanadium-Iron Prussian blue analogue (V 1/5 Fe(CN) 6 PBA) impregnated with insulin (INS), as a peroxidase-like mimetic probe toward 3,3'5,5'-tetramethylbenzidine (TMB) oxidation and colorimetric monitoring of fish freshness. A specific chemical reaction between putrescine as spoilage marker and the Vanadium-Iron V 1/5 Fe(CN) 6 PBA impregnated with insulin (V 1/5 Fe(CN) 6 PBA/INS) induces a decreasing peroxidase-like activity of the V 1/5 Fe(CN) 6 PBA/INS, generating lower amounts of oxidized products of TMB. The proposed bio-strategy achieved highly sensitive responses in the range of 30 nM to 12 μM toward putrescine with a detection limit of 9.0 nM and outstanding stability, reproducibility, and selectivity. The freshness of five different types of fish samples determined and produces a noticeable color change consistent with the stale fish around the 10 μM, which can warn the oldness of the fish. This new strategy of integration between peptide and multi-metal PBA with intensified enzyme-like mimetics activity may be highly meaningful for thoroughly monitoring the amount of fish freshness., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

A photo-induced metal-organic framework-enzyme hybrid nanosystem was developed via a controllable physical embedding method that displays dual enzymatic and photo-non-enzymatic strategy which cause high stability and cascade catalytic performance to oxidation of o-phenylenediamine and generate a UV-Vis signal at 450 nm for the tracing and sensitive detection of putrescine (Put). Under optimal conditions, the present bioassay provides a wide detection range from 0.02 to 10 µM and 20-80 µM with a detection limit of 5.5 nM, which is more desirable than numerous previous reports. In addition, the established colorimetric photo-bioassay can selectively and accurately identify Put in the presence of other distributing species. The present work provides an elegant strategy to merged photo-nanozymes' and enzyme capabilities and also broadened the sensing strategies of photo-nanozymes with promising potential in the realm of cancer diagnosis and food quality monitoring as well as its potential in various bioassays and heterogeneous catalysis fields., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

Mercaptopurine (6-MP), an immunosuppressive drug, has been widely prescribed for treating leukemia and autoimmune diseases. The level of the 6-MP drug in body fluids is of great interest due to the severe health problems related to its overdose. This study used a facile microwave preparation route to synthesize carbon quantum dots (CQDs) using glutathione and formamide as carbon sources. The obtained monodispersed quantum dots showed dual fluorescence emission with a sensitive affinity toward the 6-MP drug. The sensor's response was optimized by tuning the temperature, pH, and volume ratio of the probe. The prepared ratiometric fluorescence method showed accurate measurements for determining mercaptopurine in aqueous solutions in the concentration range of 1.4-7.6 mg L -1 with the limit of detection of 1.3 mg L -1 . The sensor's performance was assessed in complex solutions, human urine, and human plasma sample and recovery values in the range of 88-127% were obtained. The reliable dual fluorometric sensor showed promising results for 6-MP determination and potential application for the determination of other chemical and biochemical species.

Cancer is one of the most difficult diseases to treat, threatening the lives of millions of people today. So far, various methods have been used to treat cancer, each having its drawbacks. One of these methods is treatment with anticancer drugs, which unfortunately have severe side effects. One of the causes of these complications is the nonspecific effects of anticancer drugs, which attack normal cells in addition to cancer cells and damage healthy tissues. In this study, we are trying to reduce the side effects and increase the efficacy of the drug by providing smart drug delivery. The metal-organic framework (MOF) was rapidly synthesized using a microwave method and at the nanoscale. The particle size of NMOF-5 was 18-20 nm, and its surface area was 2690 m 2 ·g -1 . A chitosan polymer coating was formed on the nanocarrier after 6-mercaptopurine was introduced. The biocompatible nanocarrier exhibited a high capacity to adsorb the drug. The biocompatible nanocarrier slowly and uniformly released 96.78% of the drug in a simulated solution at pH 5 and 20.52% at pH 7.4. This showed that CS-6-MP-NMOF-5 released the drug smartly and pH-sensitively. The stability of the biocompatible nanocarrier was studied at different pH values and remained stable at pH 5 for up to 48 h. The toxicity study of the MCF-7 cell line at different concentrations for 24 h showed the excellent performance of the biocompatible nanocarrier compared to the free drug in terms of toxicity to breast cancer cells.

Breast cancer is the most common cause of cancer death in women; therefore, its early detection and treatment are crucial. To achieve this goal, we designed an optical sensor based on direct interaction of trastuzumab [Herceptin (HER)], a monoclonal antibody used to treat HER2-positive breast cancer, with plasmonic nanoparticles. Surface-modified gold nanoparticles (AuNPs) have gained considerable attention in biosensing techniques over the last years, which actuated these nanoparticles to the heart of various biosensing notions. We have exploited the localized surface plasmon resonance (LSPR) of gold nanoparticles to determine HER in human serum. AuNPs were decorated with negatively charged citrate ions, yielding enhanced direct-surface interaction with HER antibodies. The AuNPs are mixed with silver nanoparticles (AgNPs) in an optimized ratio to increase selectivity and sensitivity further. AuNPs detect the HER antibodies using LSPR, whereas AgNPs help monitor interferences' effect on the sensing media. The three effective factors in HER sensing, including the nanoparticle ratio, temperature, and pH were optimized via response surface methodology (RSM) based on the central composite design (CCD). The sensor's response toward HER was achieved in the linear range of 0.5 × 10 -7 to 40 × 10 -7 M with the detection limit of 3.7 × 10 -9 M and relative standard deviation (RSD) less than 5%. The selectivity of the LSPR sensor was assessed by monitoring its response toward HER in the presence of other biological molecules with similar physicochemical properties. Rapid response time (less than 1 min), selectivity, and the simplicity of the developed LSPR-based sensor are the key advantages of the developed sensor.

In this work, amine-carbon quantum dots (CQDs)/rhodamine B (RhB) ratiometric fluorescent (RF) sensor was employed for effective and selective determination of tamsulosin hydrochloride (TMS) based on a dual-emission fluorescence system. Although the function of amine-CQDs is to transfer the specific interaction between TMS and sensor into detectable fluorescence (FL) signals, RhB as a reference unit has been employed to omit internal and external effects. The FL signal was quenched by adding the TMS at 442 nm; nevertheless, it did not change at 569 nm. The material characterization and investigation of the sensing mechanism were done. The optimization of pH, the volumetric ratio of CQDs to RhB, and interaction time parameters were carried out by the one-variable-at-a-time (OVAT) method. The quantitative analysis of the concentration of TMS for this RF sensor in a linear range of 0.446-7.083 μg mL -1 (1.091-17.338 μM) was obtained (R 2  = 0.9969, n = 3) under optimum conditions. The limit of detection and quantitation values were estimated to be 0.033 μg mL -1 (0.081 μM) and 0.109 μg mL -1 (0.267 μM), respectively. The repeatability of intra-day and inter-day were less than one percent. This inexpensive RF probe was well applied to determine TMS in biological fluids, and acceptable achievements were obtained., (© 2021. The Author(s).)

Optical biosensors show attractive performance in medical sensing in the event of using different nanoparticles in their design. Owing to their unique optical characteristics and biological compatibility, gold nanoparticles (GNPs), silver nanoparticles (AgNPs), bimetallic nanoparticles and magnetic nanoparticles have been broadly implemented in making sensing tools. The functionalization of these nanoparticles with different components provides an excellent opportunity to assemble selective and sensitive sensing materials to detect various biological molecules related to breast cancer. This review summarizes the recent application of optical biosensing devices based on nanomaterials and discusses their pros and cons to improve breast cancer detection in real samples. In particular, the main constituent elements of these optical biosensors including recognition and transducer elements, types of applied nanostructures, analytical sensing procedures, sensor detection ranges and limit of detection (LOD), are expressed in detail., (Copyright © 2021 Elsevier B.V. All rights reserved.)

This study was carried out to project a safe nano-drug carrier composed of chitosan and cyanocobalamin (CNCbl) to improve oral delivery of ciprofloxacin hydrochloride (CIP). CIP is classified in class IV of the biopharmaceutical classification system with low solubility and permeabilityA, so it has some problems if given orally. Novel conjugate of low molecular weight chitosan, as a natural biopolymer, and CNCbl was synthesized, and then drug loading and in-vitro drug release were assessed. The loading of CIP was optimized by the Design-Expert software and the central composite design method, and that the optimal drug loading efficiency (57%) was obtained via analysis of variance (ANOVA). In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl. Functionalized nano-drug-loaded carrier showed cytotoxicity as much as that of free drug, particle size less than 100 nm as well as positive zeta potential. Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model., Competing Interests: Declaration of competing interest None., (Copyright © 2021. Published by Elsevier B.V.)

In the present study, MIL-101(Fe) and amine-carbon quantum dots (CQDs) were combined via a post-synthetic modification (PSM) method; thus, a novel MIL-101(Fe)@amine-CQD hybrid fluorescent probe sensor for the detection of 6-mercaptopurine (6-MP) was synthesized. Amine-CQDs as a fluorescent material can convert the bonding interaction between MIL-101(Fe) and 6-MP into recognizable fluorescence signals, and MIL-101 (Fe) as an adsorbent can pre-concentrate 6-MP. Hereupon, this new sensor demonstrates high selectivity and sensitivity towards the detection of 6-MP. The addition of 6-MP to this probe quenches the fluorescence signal at 599 nm. In this study, factors such as pH, response time, and concentration of MIL-101(Fe)@amine-CQDs were optimized by the one-factor-at-a-time (OFAT) method. Under optimal conditions, the relationship between the fluorescence enhancement factor and the concentration of 6-MP for this sensor in the range of 0.1667-1.0000 μg L -1 was linear (R 2 = 0.9977, n = 3). The limit of detection and limit of quantitation were 55.70 ng L -1 and 202.06 ng L -1 , respectively, which are better than similar techniques. The repeatability of intra-day and inter-day was 2.4% and 4.7%, respectively. This fluorescent sensor was employed to determine 6-MP in real samples and exhibited acceptable results.

For the determination of Metformin in human serum, a facile colorimetric and spectrophotometric sensor was designed based on citrate-capped gold nanoparticles (citrate-GNPs). In this probe, the addition of Metformin to GNP solution generates a naked-eye color change resulting from the aggregation of GNPs. Study of this color conversion and quantity analysis of analyte is operated by spectrophotometric instruments. The three factors pH, time, and GNP ratio were selected to examine their effects on sensing results and their values optimization. The optimization of parameters was done by means of central composite design and one-at-a-time methods. The sensing results proved the highly selective and sensitive performance of the sensor for Metformin in a linear range of 6.25-133.3 ppm with a detection limit of 1.79 ppm. The relative standard deviation (RSD) of the reported method is 2.53%., Competing Interests: The authors declare no competing financial interest., (Copyright © 2019 American Chemical Society.)

A highly selective and sensitive optical sensor based on localised surface plasmon resonance (LSPR) of gold nanoparticles (AuNPs) for the determination of a sulphide ion in aqueous solution is presented. The existence of sulphide is change the intensity of the LSPR band around 520 nm. In this sensor, sulphide ions were recognised and measured by UV-vis spectrophotometry. Three factors such as pH, time and concentration of AuNPs have been studied. The optimisation of effective parameters is done by one at a time method. This method was simple, rapid and cost efficient for the detection of sulphide. The linear range is 1.00-10.00 (4.16-41.63 µM) ppm with the correlation coefficient of 0.9874 and the detection limit of 0.54 ppm. The relative standard deviation of the reported method is 1.01%.

A new and simple flow injection method followed by atomic absorption spectrometry was developed for indirect determination of sulfite. The proposed method is based on the oxidation of sulfite to sulphate ion using solid-phase manganese dioxide (30% W/W suspended on silica gel beads) reactor. MnO 2 will be reduced to Mn(II) by sample injection in to the column under acidic carrier stream of HNO 3 (pH 2) with flow rate of 3.5mLmin -1 at room temperature. Absorption measurement of Mn(II) which is proportional to the concentration of sulfite in the sample was carried out by atomic absorption spectrometry. The calibration curve was linear up to 25mgL -1 with a detection limit (DL) of 0.08mgL -1 for 400µL injection sample volume. The presented method is efficient toward sulfite determination in sugar and water samples with a relative standard deviation (RSD) less than 1.2% and a sampling rate of about 60h -1 ., (Copyright © 2017 Elsevier B.V. All rights reserved.)

In this work, the synthesis of the magnetic graphene oxide modified by 2-pyridinecarboxaldehyde thiosemicarbazone groups (Fe 3 O 4 @GO/2-PTSC) was utilized for preconcentration and determination of mercuric ions in a trace amount by inductively coupled plasma-optical emission spectrometry (ICP-OES). Characterization of the adsorbent was performed using various techniques, such as FT-IR, VSM, SEM and XRD analysis. Central composite design (CCD) under response surface methodology (RSM) was used for obtaining the most important parameters and probable interactions in variables. The variables such as adsorbent dosage, pH, desorption time, and eluent volume was optimized. These values were 8mg, 5.4min, 0.5mL (HCl, 0.1M), respectively. Sonication had an important role in shortening the adsorption time of Hg (II) ions by enhancing the dispersion of adsorbent in solution. Under the optimal conditions, the proposed method presented high enrichment factor of 193, an extraction percentage of 96.5, a detection limit of 0.0079µgL -1 and a relative standard deviation (RSD %) of 1.63%. Finally, the application of the synthesized material was evaluated for preconcentration and determination of mercuric ions from foods and environmental waters samples., (Copyright © 2017 Elsevier B.V. All rights reserved.)

A selective adsorbent based on the modification of mesoprous SBA-15 with N,N'-bis(salicylidene)-1,3-ethylenediamine Schiff base and decorated with Fe 3 O 4 nanoparticles (SBA-15-BSEA-Fe 3 O 4 -NPs) for Ce(III) ions removal was reported. The SBA-15-BSEA-Fe 3 O 4 -NPs was identified by XRD, FE-SEM, TEM, SEM, FT-IR, VSM, BET and BJH analysis. Central composite design (CCD) was applied to evaluate the main and interactive effects of adsorption variables and optimize the operational parameters. The important variable such as initial pH solution, SBA-15-BSEA-Fe 3 O 4 -NPs mass, shaking time and initial concentration of Ce 3+ ions were studied under batch mode. In desirability concession of 1.0 as optimum value for R% Ce(III) , the level of factors was as follows: shaking time 80min, SBA-15-BSEA-Fe 3 O 4 -NPs mass 0.05g, pH 5 and initial concentration of Ce(III) ions 40mgL -1 . The SBA-15-BSEA-Fe 3 O 4 -NPs exhibited high adsorption efficiency and very good selectivity through cerium removal even in the presence of other ions (La 3+ , Nb 3+ , Er 3+ , Cu 2+ , Cd 2+ , Cr 3+ , and Fe 2+ ions). The SBA-15-BSEA-Fe 3 O 4 -NPs was successfully regenerated and the response was reversible. The R.S.D. of the adsorption process was less than 1.02%., (Copyright © 2017 Elsevier Inc. All rights reserved.)

A novel adsorbent, based on modifying graphene oxide (GO) chemically with 2-pyridinecarboxaldehyde thiosemicarbazone (2-PTSC) as ligand, was designed by facile process for removal of Hg(II) from aqueous solution. Characterization of the adsorbent was performed using various techniques, such as FT-IR, XRD, XPS, SEM and AFM analysis. The adsorption capacity was affected by variables such as adsorbent dosage, pH solution, Hg(2+) initial concentration and sonicating time. These variables were optimized by rotatable central composite design (CCD) under response surface methodology (RSM). The predictive model for Hg(II) adsorption was constructed and applied to find the best conditions at which the responses were maximized. In this conditions, the adsorption capacity of this adsorbent for Hg(2+) ions was calculated to be 309mgg(-1) that was higher than that of GO. Appling the ultrasound power combined with adsorption method was very efficient in shortening the removal time of Hg(2+) ions by enhancing the dispersion of adsorbent and metal ions in solution and effective interactions among them. The adsorption process was well described by second-order kinetic and Langmuir isotherm model in which the maximum adsorption capacity (Qm) was found to be 555mgg(-1) for adsorption of Hg(2+) ions over the obtained adsorbent. The performance of adsorbent was examined on the real wastewaters and confirmed the applicability of adsorbent for practical applications., (Copyright © 2016 Elsevier B.V. All rights reserved.)

In present work, a graphene oxide chemically modified with 2,2'-dipyridylamine (GO-DPA), was synthesized by simple, fast and low-cost process for the simultaneous adsorption of four toxic heavy metals, Pb(II), Cd(II), Ni(II) and Cu(II), from aqueous solutions. The synthesized adsorbent was characterized by FT-IR, XRD, XPS, SEM and AFM measurements. The effects of variables such as pH solution, initial ion concentrations, adsorbent dosage and sonicating time were investigated on adsorption efficiency by rotatable central composite design. The optimum conditions, specified as 8mg of adsorbent, 20mgL(-1) of each ion at pH 5 and short time of 4min led to the achievement of a high adsorption capacities. Ultrasonic power had important role in shortening the adsorption time of ions by enhancing the dispersion of adsorbent in solution. The adsorption kinetic studies and equilibrium isotherms for evaluating the mechanism of adsorption process showed a good fit to the pseudo-second order and Langmuir model, respectively. The maximum adsorption capacities (Qm) of this adsorbent were 369.749, 257.201, 180.893 and 358.824mgg(-1) for lead, cadmium, nickel and copper ions, respectively. The removal performance of adsorbent on the real wastewater samples also showed the feasibility of adsorbent for applying in industrial purposes., (Copyright © 2016 Elsevier B.V. All rights reserved.)

MIL-68(Al) as a metal-organic framework (MOF) was synthesized and characterized by different techniques such as SEM, BET, FTIR, and XRD analysis. This material was then applied for simulations removal of malachite green (MG) and methylene blue (MB) dyes from aqueous solutions using second order derivative spectrophotometric method (SODS) which was applied to resolve the overlap between the spectra of these dyes. The dependency of dyes removal efficiency in binary solutions was examined and optimized toward various parameters including initial dye concentration, pH of the solution, adsorbent dosage and ultrasonic contact time using central composite design (CCD) under response surface methodology (RSM) approach. The optimized experimental conditions were set as pH7.78, contact time 5min, initial MB concentration 22mgL(-1), initial MG concentration 12mgL(-1) and adsorbent dosage 0.0055g. The equilibrium data was fitted to isotherm models such as Langmuir, Freundlich and Tempkin and the results revealed the suitability of the Langmuir model. The maximum adsorption capacity of 666.67 and 153.85mgg(-1) was obtained for MB and MG removal respectively. Kinetics data fitting to pseudo-first order, pseudo-second order and Elovich models confirmed the applicability of pseudo-second order kinetic model for description of the mechanism and adsorption rate. Dye-loaded MIL-68(Al) can be easily regenerated using methanol and applied for three frequent sorption/desorption cycles with high performance. The impact of ionic strength on removal percentage of both dyes in binary mixture was studied by using NaCl and KCl soluble salts at different concentrations. According to our findings, only small dosage of the proposed MOF is considerably capable to remove large amounts of dyes at room temperature and in very short time that is a big advantage of MIL-68(Al) as a promising adsorbent for adsorptive removal processes., (Copyright © 2016 Elsevier B.V. All rights reserved.)

In this study, a rapid flow injection-flame atomic absorption spectrometry for cyanide detection was developed. Different AgX (where X is Cl(-), Br(-), I(-) and N(3)(-)) solid-phase reagents (SPR) were tested for indirect determination of cyanide. In a single-line FIA system, the cyanide was allowed to react with AgX SPR, which in turn changed Ag ions in AgX to silver cyanide complexes in a sodium hydroxide carrier stream. The eluent containing the analyte as silver cyanide complexes was measured by FAAS. The calibration curve was linear up to 30 mg l(-1) with a detection limit of 0.05 mg l(-1) for cyanides. The sampling rate and the relative standard deviation were <1.09% and >200 h(-1), respectively. The method was applied to the determination of cyanide in electroplating wastewater.

Four solid-phase reagents have been tested for indirect determination of cyanide using flow injection analysis-flame atomic absorption spectrometry (FIA-FAAS). The method is based on insertion of aqueous cyanide solutions into an on-line Ag(2)X (where X are SO(3)(2-), Cr(2)O(7)(2-), C(2)O(4)(2-) and CO(3)(2-)) packed column (25%, m/m suspended on silica gel beads) and re-distilled water or sodium hydroxide are used as the carrier stream. The eluent containing the analyte as silver cyanide complexes, produced from reaction between Ag(2)X and cyanide, measured by flame atomic absorption spectrometry. The method is simple, fast and selective than other published FIA procedures. A relative standard deviation (R.S.D.) better than 1.12% was obtained in a repeatability study. The method was applied to the determination of cyanide in industrial electrolytic baths.