Search

SUMMARY: The indiscriminate use of anabolic steroids in gyms has been growing in a generalized way, among which, the most common is growth hormone (GH). In the short term GH may potentiate muscle growth, especially when taken in combination with resistance training. However, the effects of this hormone are not yet fully understood in the literature, especially in relation to collagen properties. The objective of this study was to evaluate the effect of the application of growth hormone (GH) and resistance training (RT) on the collagen properties of femoral bone tissue using Raman Spectroscopy. In this study 40 male rats were randomly distributed into four groups (n=10): control (C), control and GH application (GH), resistance training (T), and resistance training and GH application (GHT). The training consisted of four series of 10 water jumps, performed three times a week, with an overload corresponding to 50 % of body weight and duration of four weeks. GH was applied at a dosage of 0.2 IU/Kg (0.067 mg/kg) to each animal, three times a week, every other day. The animals were euthanized and the right femurs were collected for analysis of bone structure. Raman spectroscopy (RS) was used to observe the following compounds from their respective bands: type I collagen (662 cm-1), amide III (1243 cm-1), proteins including type I collagen (1278 cm-1), woven collagen (1322 cm-1), association of collagen, phospholipids, nucleic acid, and phosphate (1330 cm-1), and collagen and protein deformation (1448 cm-1). The results demonstrated an increase in the collagen properties in all analyzed variables, however, the T group presented a statistically significant difference (p

SUMMARY: The objective of this study was to evaluate the effects of growth hormone (GH) and muscle strength training (ST) on the composition of bone tissue of Wistar rats through Raman spectroscopy. In total, 40 male rats were randomly distributed into four groups: (N = 10) control (C), control with the application of GH (GHC), strength training (T), and strength training with the application of GH (GHT). The training consisted of four series of 10 water jumps, performed three times a week, with an overload corresponding to 50 % of body weight and duration of four weeks. GH was applied at a dose of 0.2 IU / kg in each animal three times a week and every other day. After four weeks, the animals were euthanized and the right femurs collected for analysis of the bone structure. Raman spectroscopy (ER) was used to observe the following compounds from their respective bands: Calcium Carbonate-Triglycerides (fatty acids) 1073 cm-1, Collagen type I 509 cm-1, Bone-DNA Phosphate (Protein) 589 cm-1, Phosphate Phospholipids 1078 cm-1. For the statistical analysis, the Shapiro-Wilk and ANOVA One-Way variance analysis normality tests were performed, followed by the Tukey post-test. The results showed an increase in the concentrations of calcium carbonate-triglycerides (fatty acids), type I collagen, bone phosphate-DNA (protein), and phosphate phospholipids in all experimental groups, with or without ST and/or GH , But only the isolated training group differed significantly from the control group (P

Submitted by Jakeline Ortega (jakortega@unoeste.br) on 2020-10-26T20:21:53Z No. of bitstreams: 2 Alessandro Pierucci.pdf: 1388988 bytes, checksum: 0fe31650826e6c958c89ff132cd3876d (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Made available in DSpace on 2020-10-26T20:21:53Z (GMT). No. of bitstreams: 2 Alessandro Pierucci.pdf: 1388988 bytes, checksum: 0fe31650826e6c958c89ff132cd3876d (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2020-05-05 Introduction: Smartphone applications for health (M-Health) seem to overcome barriers to access to Cardiovascular Rehabilitation Programs (CRP), favored by their high degree of acceptability and can positively influence the frequency of physical exercise in weight loss. Objective: Analyze the influence of the association between M-Health and CRP in cardiorespiratory fitness, adherence to CRP and in management of cardiovascular risk factors, when compared to cardiac patients inserted in isolated CRP (without association with M-Health). Design: Systematic Review and Meta-Analysis Methods: The following databases were used: Medline via Ovid, EMBASE, Central, PEDro and SPORTDiscus via EBSCOhost from the oldest record until December 20, 2019. Among the inclusion criteria are cardiac patients older than 18 years submitted to M- Health associated with CRP. Results: 14 randomized controlled trials (RCTs) were eligible. The risk of bias was considered moderate and the interventions with M-Health consisted of text messages, e-mails, and applications. In the primary endpoint, thirteen of these studies assessed VO2 peak, 6MWT and IPAQ; in secondary outcomes, eight studies addressed cardiovascular risk management and nine assessed adherences to interventions and feedback questionnaires. For quantitative analysis, there were estimates of effects favorable to the association of M-Health for the variables: cardiorespiratory fitness, resting heart rate, diastolic blood pressure and triglycerides. Conclusion: There is high quality of evidence and strength of recommendation favorable to the intervention of M-Health in improving cardiorespiratory fitness and triglycerides. This favorable effect extends to the behavior of heart rate and diastolic blood pressure, despite lower levels of quality of evidence. Introdução: Aplicativos de smatphones para saúde (M-Health) parecem superar barreiras ao acesso a Programas de Reabilitação Cardiovascular (PRCV), favorecido pelo seu alto grau de aceitabilidade e podendo influenciar positivamente na frequência do exercício físico. Objetivo: Analisar evidências sobre os benefícios da associação entre o uso de M-Health e PRCV nos desfechos primários e secundários (qualidade de vida, aderência e manejo de fatores de risco cardiovasculares). Desenho: Revisão Sistemática e Metanálise. Métodos: Foram utilizadas as bases de dados: PubMed, EMBASE e SPORTDiscus com texto completo (EBSCOhost) a partir do registro mais antigo até o dia 20 de abril de 2018. Entre os critérios de inclusão estão cardiopatas maiores de 18 anos submetidos ao M-Health associado ao PRCV. Resultados: 8 ensaios clinicos randomizados (ECR) foram elegíveis. O risco de viés foi considerado moderado e as intervenções com M-Health consistiram em mensagem de texto, e-mails e por aplicativos. Nos desfechos primários seis desses estudos avaliaram VO2pico, TC6min e IPAQ em curto e intermediário prazo. Nos desfechos secundários, seis estudos abordaram a aderência as intervenções, questionários de feedback e qualidade de vida. Para análise quantitativa houveram estimativa de efeito favorável a associação do M-Health e PRCV para as variáveis aptidão cardiorrespiratória, frequência cardíaca, colesterol total, LDL e triglicerídes. Conclusão: Há alta qualidade de evidência e força de recomendação favorável associação da intervenção do M-Health ao PRCV na melhora da aptidão cardiorrespiratória a curto prazo, e colesterol total e triglicérides para o período intermediário, já para frequência cardíaca e LDL a evidência é com fraca força de recomendação.