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Training, as a core device in the projects that use sport to increase employability, is today a much-cited element in papers in the sector. However, there seems to be little research that specifically delves into training processes. This contribution analyses the state of the art on the subject, focusing on the characteristics of the training courses mentioned in literature, highlighting some recurring critical issues. A proposal, which considers the limitations discussed above, is formulated as a result of this analysis. More specifically, we introduce, as a contribution to the debate, a training model for team sports coaches developed within the EU Erasmus + sport project SBSMED. Theoretical assumptions, methodology, contents and assessment methods of training effectiveness will be explained, highlighting valuable elements and the open issues that emerge from this experience., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (© 2023 Gozzoli, Palumbo and Zanoli.)

The Differential Display Reverse Transcriptase (DDRT) technique was adopted to isolate genetic markers specific for the two main grades of the Glial tumor, the Astrocytoma and the Glioblastoma. A total of 16 brain biopsies (4 Astrocytoma and 12 Glioblastoma) were analysed. The technique was modified in order to reduce the false-positive ratio by means of more stringent amplification conditions. Electrophoretic patterns with previously selected arbitrarily primers revealed differences between the grades, four of them were investigated through sequencing. These sequences did not show significant nucleotide and aminoacid similarity to any known sequences in the DataBase. Sensitivity of the method was documented by the evidence that only one of the selected markers was an artefact, while the others represented genetic markers of the human Glial neoplasm.