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2. Imaging interorganelle contacts at a glance.

Author

Zanellati, Maria Clara, Chih-Hsuan Hsu, and Cohen, Sarah

Abstract

Eukaryotic cells are compartmentalized into membrane-bound organelles that must coordinate their responses to stimuli. One way that organelles communicate is via membrane contact sites (MCSs), sites of close apposition between organelles used for the exchange of ions, lipids and information. In this Cell Science at a Glance article and the accompanying poster, we describe an explosion of new methods that have led to exciting progress in this area and discuss key examples of how these methods have advanced our understanding of MCSs. We discuss how diffraction-limited and super-resolution fluorescence imaging approaches have provided important insight into the biology of interorganelle communication.We also describe how the development of multiple proximity-based methods has enabled the detection ofMCSswith high accuracy and precision. Finally, we assess howrecent advances in electronmicroscopy (EM), considered the gold standard for detecting MCSs, have allowed the visualization of MCSs and associated proteins in 3D at ever greater resolution. [ABSTRACT FROM AUTHOR]

Published
2024
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8. A reference human induced pluripotent stem cell line for large-scale collaborative studies

Author

Pantazis, Caroline B., primary, Yang, Andrian, additional, Lara, Erika, additional, McDonough, Justin A., additional, Blauwendraat, Cornelis, additional, Peng, Lirong, additional, Oguro, Hideyuki, additional, Kanaujiya, Jitendra, additional, Zou, Jizhong, additional, Sebesta, David, additional, Pratt, Gretchen, additional, Cross, Erin, additional, Blockwick, Jeffrey, additional, Buxton, Philip, additional, Kinner-Bibeau, Lauren, additional, Medura, Constance, additional, Tompkins, Christopher, additional, Hughes, Stephen, additional, Santiana, Marianita, additional, Faghri, Faraz, additional, Nalls, Mike A., additional, Vitale, Daniel, additional, Ballard, Shannon, additional, Qi, Yue A., additional, Ramos, Daniel M., additional, Anderson, Kailyn M., additional, Stadler, Julia, additional, Narayan, Priyanka, additional, Papademetriou, Jason, additional, Reilly, Luke, additional, Nelson, Matthew P., additional, Aggarwal, Sanya, additional, Rosen, Leah U., additional, Kirwan, Peter, additional, Pisupati, Venkat, additional, Coon, Steven L., additional, Scholz, Sonja W., additional, Priebe, Theresa, additional, Öttl, Miriam, additional, Dong, Jian, additional, Meijer, Marieke, additional, Janssen, Lara J.M., additional, Lourenco, Vanessa S., additional, van der Kant, Rik, additional, Crusius, Dennis, additional, Paquet, Dominik, additional, Raulin, Ana-Caroline, additional, Bu, Guojun, additional, Held, Aaron, additional, Wainger, Brian J., additional, Gabriele, Rebecca M.C., additional, Casey, Jackie M., additional, Wray, Selina, additional, Abu-Bonsrah, Dad, additional, Parish, Clare L., additional, Beccari, Melinda S., additional, Cleveland, Don W., additional, Li, Emmy, additional, Rose, Indigo V.L., additional, Kampmann, Martin, additional, Calatayud Aristoy, Carles, additional, Verstreken, Patrik, additional, Heinrich, Laurin, additional, Chen, Max Y., additional, Schüle, Birgitt, additional, Dou, Dan, additional, Holzbaur, Erika L.F., additional, Zanellati, Maria Clara, additional, Basundra, Richa, additional, Deshmukh, Mohanish, additional, Cohen, Sarah, additional, Khanna, Richa, additional, Raman, Malavika, additional, Nevin, Zachary S., additional, Matia, Madeline, additional, Van Lent, Jonas, additional, Timmerman, Vincent, additional, Conklin, Bruce R., additional, Johnson Chase, Katherine, additional, Zhang, Ke, additional, Funes, Salome, additional, Bosco, Daryl A., additional, Erlebach, Lena, additional, Welzer, Marc, additional, Kronenberg-Versteeg, Deborah, additional, Lyu, Guochang, additional, Arenas, Ernest, additional, Coccia, Elena, additional, Sarrafha, Lily, additional, Ahfeldt, Tim, additional, Marioni, John C., additional, Skarnes, William C., additional, Cookson, Mark R., additional, Ward, Michael E., additional, and Merkle, Florian T., additional

Published
2022
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9. A reference human induced pluripotent stem cell line for collaborative studies

Author

Pantazis, Caroline B, Yang, Andrian, Lara, Erika, McDonough, Justin A, Blauwendraat, Cornelis, Peng, Lirong, Oguro, Hideyuki, Kanaujiya, Jitendra, Zou, Jizhong, Sebesta, David, Pratt, Gretchen, Cross, Erin, Blockwick, Jeffrey, Buxton, Philip, Kinner-Bibeau, Lauren, Medura, Constance, Tompkins, Christopher, Hughes, Stephen, Santiana, Marianita, Faghri, Faraz, Nalls, Mike A, Vitale, Daniel, Ballard, Shannon, Qi, Yue A, Ramos, Daniel M, Anderson, Kailyn M, Stadler, Julia, Narayan, Priyanka, Papademetriou, Jason, Reilly, Luke, Nelson, Matthew P, Aggarwal, Sanya, Rosen, Leah U, Kirwan, Peter, Pisupati, Venkat, Coon, Steven L, Scholz, Sonja W, Priebe, Theresa, Ottl, Miriam, Dong, Jian, Meijer, Marieke, Janssen, Lara JM, Lourenco, Vanessa S, van der Kant, Rik, Crusius, Dennis, Paquet, Dominik, Raulin, Ana-Caroline, Bu, Guojun, Held, Aaron, Wainger, Brian J, Gabriele, Rebecca MC, Casey, Jackie M, Wray, Selina, Abu-Bonsrah, Dad, Parish, Clare L, Beccari, Melinda S, Cleveland, Don W, Li, Emmy, Rose, Indigo VL, Kampmann, Martin, Aristoy, Carles Calatayud, Verstreken, Patrik, Heinrich, Laurin, Chen, Max Y, Schule, Birgitt, Dou, Dan, Holzbaur, Erika LF, Zanellati, Maria Clara, Basundra, Richa, Deshmukh, Mohanish, Cohen, Sarah, Khanna, Richa, Raman, Malavika, Nevin, Zachary S, Matia, Madeline, Van Lent, Jonas, Timmerman, Vincent, Conklin, Bruce R, Chase, Katherine Johnson, Zhang, Ke, Funes, Salome, Bosco, Daryl A, Erlebach, Lena, Welzer, Marc, Kronenberg-Versteeg, Deborah, Lyu, Guochang, Arenas, Ernest, Coccia, Elena, Sarrafha, Lily, Ahfeldt, Tim, Marioni, John C, Skarnes, William C, Cookson, Mark R, Ward, Michael E, and Merkle, Florian T

Subjects
Science & Technology, Cell Biology, DOPAMINE NEURONS, RISK LOCI, GENE, DISEASE, DIRECTIONAL GENOMIC HYBRIDIZATION, COPY NUMBER, DIFFERENTIATION, Cell & Tissue Engineering, HETEROGENEITY, Life Sciences & Biomedicine, A-BETA, GENERATION
Abstract

Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across research groups. To address this issue, we sub-cloned candidate human iPSC lines and deeply characterized their genetic properties using whole genome sequencing, their genomic stability upon CRISPR-Cas9-based gene editing, and their phenotypic properties including differentiation to commonly used cell types. These studies identified KOLF2.1J as an all-around well-performing iPSC line. We then shared KOLF2.1J with groups around the world who tested its performance in head-to-head comparisons with their own preferred iPSC lines across a diverse range of differentiation protocols and functional assays. On the strength of these findings, we have made KOLF2.1J and its gene-edited derivative clones readily accessible to promote the standardization required for large-scale collaborative science in the stem cell field. ispartof: CELL STEM CELL vol:29 issue:12 pages:1685-+ ispartof: location:United States status: published

Published
2022

10. A reference induced pluripotent stem cell line for large-scale collaborative studies

Author

Pantazis, Caroline B., primary, Yang, Andrian, additional, Lara, Erika, additional, McDonough, Justin A., additional, Blauwendraat, Cornelis, additional, Peng, Lirong, additional, Oguro, Hideyuki, additional, Kanaujiya, Jitendra, additional, Zou, Jizhong, additional, Sebesta, David, additional, Pratt, Gretchen, additional, Cross, Erin, additional, Blockwick, Jeffrey, additional, Buxton, Philip, additional, Kinner-Bibeau, Lauren, additional, Medura, Constance, additional, Tompkins, Christopher, additional, Hughes, Stephen, additional, Santiana, Marianita, additional, Faghri, Faraz, additional, Nalls, Mike A., additional, Vitale, Daniel, additional, Ballard, Shannon, additional, Qi, Yue A., additional, Ramos, Daniel M., additional, Anderson, Kailyn M., additional, Stadler, Julia, additional, Narayan, Priyanka, additional, Papademetriou, Jason, additional, Reilly, Luke, additional, Nelson, Matthew P., additional, Aggarwal, Sanya, additional, Rosen, Leah U., additional, Kirwan, Peter, additional, Pisupati, Venkat, additional, Coon, Steven L., additional, Scholz, Sonja W., additional, Priebe, Theresa, additional, Öttl, Miriam, additional, Dong, Jian, additional, Meijer, Marieke, additional, Janssen, Lara J.M., additional, Lourenco, Vanessa S., additional, van der Kant, Rik, additional, Crusius, Dennis, additional, Paquet, Dominik, additional, Raulin, Ana-Caroline, additional, Bu, Guojun, additional, Held, Aaron, additional, Wainger, Brian J., additional, Gabriele, Rebecca M.C., additional, Casey, Jackie M, additional, Wray, Selina, additional, Abu-Bonsrah, Dad, additional, Parish, Clare L., additional, Beccari, Melinda S., additional, Cleveland, Don W., additional, Li, Emmy, additional, Rose, Indigo V.L., additional, Kampmann, Martin, additional, Aristoy, Carles Calatayud, additional, Verstreken, Patrik, additional, Heinrich, Laurin, additional, Chen, Max Y., additional, Schüle, Birgitt, additional, Dou, Dan, additional, Holzbaur, Erika L.F., additional, Zanellati, Maria Clara, additional, Basundra, Richa, additional, Deshmukh, Mohanish, additional, Cohen, Sarah, additional, Khanna, Richa, additional, Raman, Malavika, additional, Nevin, Zachary S., additional, Matia, Madeline, additional, Lent, Jonas Van, additional, Timmerman, Vincent, additional, Conklin, Bruce R., additional, Chase, Katherine Johnson, additional, Zhang, Ke, additional, Funes, Salome, additional, Bosco, Daryl A., additional, Erlebach, Lena, additional, Welzer, Marc, additional, Kronenberg-Versteeg, Deborah, additional, Lyu, Guochang, additional, Arenas, Ernest, additional, Coccia, Elena, additional, Sarrafha, Lily, additional, Ahfeldt, Tim, additional, Marioni, John C., additional, Skarnes, William C., additional, Cookson, Mark R., additional, Ward, Michael E., additional, and Merkle, Florian T., additional

Published
2021
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13. APOE traffics to astrocyte lipid droplets and modulates triglyceride saturation and droplet size.

Author

Windham, Ian A., Powers, Alex E., Ragusa, Joey V., Wallace, E. Diane, Zanellati, Maria Clara, Williams, Victoria H., Wagner, Colby H., White, Kristen K., and Cohen, Sarah

Subjects
*DISEASE risk factors, *LIPIDS, *ALZHEIMER'S disease, *APOLIPOPROTEIN E4, *TRIGLYCERIDES
Abstract

The E4 variant of APOE strongly predisposes individuals to late-onset Alzheimer's disease. We demonstrate that in response to lipogenesis, apolipoprotein E (APOE) in astrocytes can avoid translocation into the endoplasmic reticulum (ER) lumen and traffic to lipid droplets (LDs) via membrane bridges at ER-LD contacts. APOE knockdown promotes fewer, larger LDs after a fatty acid pulse, which contain more unsaturated triglyceride after fatty acid pulse-chase. This LD size phenotype was rescued by chimeric APOE that targets only LDs. Like APOE depletion, APOE4-expressing astrocytes form a small number of large LDs enriched in unsaturated triglyceride. Additionally, the LDs in APOE4 cells exhibit impaired turnover and increased sensitivity to lipid peroxidation. Our data indicate that APOE plays a previously unrecognized role as an LD surface protein that regulates LD size and composition. APOE4 causes aberrant LD composition and morphology. Our study contributes to accumulating evidence that APOE4 astrocytes with large, unsaturated LDs are sensitized to lipid peroxidation, which could contribute to Alzheimer's disease risk. [ABSTRACT FROM AUTHOR]

Published
2024
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