Search

Your search keyword '"Zamudio, Roxana"' showing total 38 results
Start Over Author "Zamudio, Roxana"
38 results on '"Zamudio, Roxana"'

3. The genetic structure and adaptation of Andean highlanders and Amazonians are influenced by the interplay between geography and culture

Author

Borda, Víctor, Alvim, Isabela, Mendes, Marla, Silva-Carvalho, Carolina, Soares-Souza, Giordano B., Leal, Thiago P., Furlan, Vinicius, Scliar, Marilia O., Zamudio, Roxana, Zolini, Camila, Araújo, Gilderlanio S., Luizon, Marcelo R., Padilla, Carlos, Cáceres, Omar, Levano, Kelly, Sánchez, César, Trujillo, Omar, Flores-Villanueva, Pedro O., Dean, Michael, Fuselli, Silvia, Machado, Moara, Romero, Pedro E., Tassi, Francesca, Yeager, Meredith, O’Connor, Timothy D., Gilman, Robert H., Tarazona-Santos, Eduardo, and Guio, Heinner

Published
2020

4. Whole-genome analysis uncovers loss of blaZ associated with carriage isolates belonging to methicillin-resistant Staphylococcus aureus (MRSA) clone ST5-VI in Cape Verde

Author

Wysocka, Magdalena, Monteiro, Tamar, de Pina, Carine, Gonçalves, Deisy, de Pina, Sandrine, Ludgero-Correia, Antonio, Moreno, Joao, Zamudio, Roxana, Almebairik, Nada, Gray, Laura J., Pareek, Manish, Jenkins, David R., Aires-de-Sousa, Marta, De Lencastre, Herminia, Beleza, Sandra, Araujo, Isabel I., Conceição, Teresa, and Oggioni, Marco R.

Published
2021
Full Text
View/download PDF

7. Origin and dynamics of admixture in Brazilians and its effect on the pattern of deleterious mutations

Author

The Brazilian EPIGEN Project Consortium, Kehdy, Fernanda S. G., Gouveia, Mateus H., Machado, Moara, Magalhães, Wagner C. S., Horimoto, Andrea R., Horta, Bernardo L., Moreira, Rennan G., Leal, Thiago P., Scliar, Marilia O., Soares-Souza, Giordano B., Rodrigues-Soares, Fernanda, Araújo, Gilderlanio S., Zamudio, Roxana, Anna, Hanaisa P. Sant, Santos, Hadassa C., Duarte, Nubia E., Fiaccone, Rosemeire L., Figueiredo, Camila A., Silva, Thiago M., Costa, Gustavo N. O., Beleza, Sandra, Berg, Douglas E., Cabrera, Lilia, Debortoli, Guilherme, Duarte, Denise, Ghirotto, Silvia, Gilman, Robert H., Gonçalves, Vanessa F., Marrero, Andrea R., Muniz, Yara C., Weissensteiner, Hansi, Yeager, Meredith, Rodrigues, Laura C., Barreto, Mauricio L., Lima-Costa, M. Fernanda, Pereira, Alexandre C., Rodrigues, Maíra R., and Tarazona-Santos, Eduardo

Published
2015

9. Population, Epidemiological, and Functional Genetics of Gastric Cancer Candidate Genes in Peruvians with Predominant Amerindian Ancestry

Author

Zamudio, Roxana, Pereira, Latife, Rocha, Carolina D., Berg, Douglas E., Muniz-Queiroz, Thaís, Sant Anna, Hanaisa P., Cabrera, Lilia, Combe, Juan M., Herrera, Phabiola, Jahuira, Martha H., Leão, Felipe B., Lyon, Fernanda, Prado, William A., Rodrigues, Maíra R., Rodrigues-Soares, Fernanda, Santolalla, Meddly L., Zolini, Camila, Silva, Aristóbolo M., Gilman, Robert H., Tarazona-Santos, Eduardo, and Kehdy, Fernanda S. G.

Published
2016
Full Text
View/download PDF

10. Dynamics of Salmonella enterica and antimicrobial resistance in the Brazilian poultry industry and global impacts on public health

Author

Alikhan, Nabil-Fareed, primary, Moreno, Luisa Zanolli, additional, Castellanos, Luis Ricardo, additional, Chattaway, Marie Anne, additional, McLauchlin, Jim, additional, Lodge, Martin, additional, O’Grady, Justin, additional, Zamudio, Roxana, additional, Doughty, Emma, additional, Petrovska, Liljana, additional, Cunha, Marcos Paulo Vieira, additional, Knöbl, Terezinha, additional, Moreno, Andrea Micke, additional, and Mather, Alison E., additional

Published
2022
Full Text
View/download PDF

14. Lineage‐specific evolution and gene flow in Listeria monocytogenes are independent of bacteriophages

Author

Zamudio, Roxana, Haigh, Richard D, Ralph, Joseph D, De Ste Croix, Megan, Tasara, Taurai, Zurfluh, Katrin, Kwun, Min Jung, Millard, Andrew D, Bentley, Stephen D, Croucher, Nicholas J, Stephan, Roger, Oggioni, Marco R, University of Zurich, and Oggioni, Marco R

Subjects
1105 Ecology, Evolution, Behavior and Systematics, Ecology, Behavior and Systematics, Evolution, 2404 Microbiology, 570 Life sciences, biology, 610 Medicine & health, Microbiology, 10082 Institute of Food Safety and Hygiene
Published
2020

17. Diversity of blaCTX-M-1-carrying plasmids recovered from Escherichia coli isolated from Canadian domestic animals.

Author

Cormier, Ashley C., Chalmers, Gabhan, Zamudio, Roxana, Mulvey, Michael R., Mather, Alison E., and Boerlin, Patrick

Subjects
DOMESTIC animals, PLASMIDS, ESCHERICHIA coli, HORSE racetracks, BEEF cattle, GENETIC variation, POULTRY, HORSE breeds
Abstract

Conserved IncI1 and IncHI1 plasmids carrying blaCTX-M-1 have been found circulating in chickens and horses from continental Europe, respectively. In Canada, blaCTX-M-1 is overwhelmingly the most common blaCTX-M variant found in Escherichia coli from chicken and horses and can be recovered at lower frequencies in swine, cattle, and dogs. Whole-genome sequencing has identified a large genetic diversity of isolates carrying this variant, warranting further investigations into the plasmids carrying this gene. Therefore, the objective of this study was to describe the genetic profiles of blaCTX-M-1 plasmids circulating in E. coli from Canadian domestic animals and compare them to those recovered in animals in Europe. Fifty-one blaCTX-M-1 positive E. coli isolates from chicken (n = 14), horses (racetrack horses n = 11; community horses n = 3), swine (n = 7), turkey (n = 6), dogs (n = 5), beef cattle (n = 3), and dairy cattle (n = 2) were selected for plasmid characterization. Sequences were obtained through both Illumina and Oxford Nanopore technologies. Genomes were assembled using either Unicycler hybrid assembly or Flye with polishing performed using Pilon. blaCTX-M-1 was found residing on a plasmid in 45 isolates and chromosomally located in six isolates. A conserved IncI1/ST3 plasmid was identified among chicken (n = 12), turkey (n = 4), swine (n = 6), dog (n = 2), and beef cattle (n = 2) isolates. When compared against publicly available data, these plasmids showed a high degree of similarity to those identified in isolates from poultry and swine in Europe. These results suggest that an epidemic IncI1/ST3 plasmid similar to the one found in Europe is contributing to the spread of blaCTX-M-1 in Canada. A conserved IncHI1/FIA(HI1)/ST2 plasmid was also recovered from nearly all racetrack horse isolates (n = 10). Although IncHI1/ST2 plasmids have been reported among European horse isolates, IncHI1/ST9 plasmids appear to be more widespread. Further studies are necessary to understand the factors contributing to these plasmids' success in their respective populations. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

18. Lineage‐specific evolution and gene flow in Listeria monocytogenes are independent of bacteriophages

Author

Zamudio, Roxana, primary, Haigh, Richard D., additional, Ralph, Joseph D., additional, De Ste Croix, Megan, additional, Tasara, Taurai, additional, Zurfluh, Katrin, additional, Kwun, Min Jung, additional, Millard, Andrew D., additional, Bentley, Stephen D., additional, Croucher, Nicholas J., additional, Stephan, Roger, additional, and Oggioni, Marco R., additional

Published
2020
Full Text
View/download PDF

19. Origins, Admixture Dynamics, and Homogenization of the African Gene Pool in the Americas

Author

Gouveia, Mateus H, primary, Borda, Victor, primary, Leal, Thiago P, primary, Moreira, Rennan G, primary, Bergen, Andrew W, primary, Kehdy, Fernanda S G, primary, Alvim, Isabela, primary, Aquino, Marla M, primary, Araujo, Gilderlanio S, primary, Araujo, Nathalia M, primary, Furlan, Vinicius, primary, Liboredo, Raquel, primary, Machado, Moara, primary, Magalhaes, Wagner C S, primary, Michelin, Lucas A, primary, Rodrigues, Maíra R, primary, Rodrigues-Soares, Fernanda, primary, Sant Anna, Hanaisa P, primary, Santolalla, Meddly L, primary, Scliar, Marília O, primary, Soares-Souza, Giordano, primary, Zamudio, Roxana, primary, Zolini, Camila, primary, Bortolini, Maria Catira, primary, Dean, Michael, primary, Gilman, Robert H, primary, Guio, Heinner, primary, Rocha, Jorge, primary, Pereira, Alexandre C, primary, Barreto, Mauricio L, primary, Horta, Bernardo L, primary, Lima-Costa, Maria F, primary, Mbulaiteye, Sam M, primary, Chanock, Stephen J, primary, Tishkoff, Sarah A, primary, Yeager, Meredith, primary, and Tarazona-Santos, Eduardo, primary

Published
2020
Full Text
View/download PDF

21. The genetic structure and adaptation of Andean highlanders and Amazonian dwellers is influenced by the interplay between geography and culture

Author

Borda, Victor, primary, Alvim, Isabela, additional, Aquino, Marla M, additional, Silva, Carolina, additional, Soares-Souza, Giordano B, additional, Leal, Thiago P, additional, Scliar, Marilia O, additional, Zamudio, Roxana, additional, Zolini, Camila, additional, Padilla, Carlos, additional, Cáceres, Omar, additional, Levano, Kelly, additional, Sanchez, Cesar, additional, Trujillo, Omar, additional, Flores-Villanueva, Pedro O., additional, Dean, Michael, additional, Fuselli, Silvia, additional, Machado, Moara, additional, Romero, Pedro E., additional, Tassi, Francesca, additional, Yeager, Meredith, additional, O’Connor, Timothy D., additional, Gilman, Robert H, additional, Tarazona-Santos, Eduardo, additional, and Guio, Heinner, additional

Published
2020
Full Text
View/download PDF

23. Origins, admixture dynamics and homogenization of the African gene pool in the Americas

Author

Gouveia, Mateus H., primary, Borda, Victor, additional, Leal, Thiago P., additional, Moreira, Rennan G., additional, Bergen, Andrew W., additional, Aquino, Marla M., additional, Araujo, Gilderlanio S., additional, Araujo, Nathalia M., additional, Kehdy, Fernanda S.G., additional, Liboredo, Raquel, additional, Machado, Moara, additional, Magalhaes, Wagner C.S., additional, Michelin, Lucas A., additional, Rodrigues, Maíra R., additional, Rodrigues-Soares, Fernanda, additional, Sant Anna, Hanaisa P., additional, Santolalla, Meddly L., additional, Scliar, Marília O., additional, Soares-Souza, Giordano, additional, Zamudio, Roxana, additional, Zolini, Camila, additional, Dean, Michael, additional, Gilman, Robert H., additional, Guio, Heinner, additional, Rocha, Jorge, additional, Pereira, Alexandre C., additional, Barreto, Mauricio L., additional, Horta, Bernardo L., additional, Lima-Costa, Maria F., additional, Mbulaiteye, Sam M., additional, Chanock, Stephen J., additional, Tishkoff, Sarah A., additional, Yeager, Meredith, additional, and Tarazona-Santos, Eduardo, additional

Published
2019
Full Text
View/download PDF

24. Lineage-specific evolution in Listeria monocytogenes detected by analysis of a panel of Swiss isolates from food and human origin

Author

Zamudio, Roxana, primary, Ralph, Joseph D, additional, Haigh, Richard D, additional, De Ste Croix, Megan, additional, Tasara, Taurai, additional, Zurfluh, Katrin, additional, Kwun, Min Jung, additional, Croucher, Nicolas J, additional, Bentley, Stephen D, additional, Stephan, Roger, additional, and Oggioni, Marco R, additional

Published
2019
Full Text
View/download PDF

26. Whole-genome analysis uncovers loss of blaZassociated with carriage isolates belonging to methicillin-resistant Staphylococcus aureus(MRSA) clone ST5-VI in Cape Verde

Author

Wysocka, Magdalena, Monteiro, Tamar, de Pina, Carine, Gonçalves, Deisy, de Pina, Sandrine, Ludgero-Correia, Antonio, Moreno, Joao, Zamudio, Roxana, Almebairik, Nada, Gray, Laura J., Pareek, Manish, Jenkins, David R., Aires-de-Sousa, Marta, De Lencastre, Herminia, Beleza, Sandra, Araujo, Isabel I., Conceição, Teresa, and Oggioni, Marco R.

Abstract

•One of the first whole genome analyses of Staphylococcus aureuscarriage isolates in an African country.•Genome data allowed to place S. aureusisolates from Cape Verde in a phylogenetic context.•Loss of blaZ-carrying plasmids and transposons is not rare, which is also evident in other international MRSA isolates.•Steady increase in antimicrobial drug resistance in Cape Verde.•Data provide genomic information for the design of intervention measures to decrease antimicrobial resistance.

Published
2021
Full Text
View/download PDF

27. Planeamiento estratégico para la industria peruana de productos orgánicos de tocador

Author

Altez Villanueva, Lorena Violeta, Euribe Chumbiauca, Luis Felipe, Salazar Neyra, Fernando, Sotelo Zamudio, Roxana Sthefany, and Aguilar Rengifo, Juan Manuel

Subjects
Planificación estratégica, Cosméticos -- Industria y comercio -- Perú
Abstract

En los últimos años, la Industria de Productos de Tocador, se ha convertido en un sector estratégico para el desarrollo de la economía del país. Ésta genera un alto valor agregado y es impulsada por el creciente comercio internacional. Sin embargo, en el Perú no se puede hablar de una industria totalmente activa, específicamente por dos razones; (a) la tendencia de crecimiento que presenta la categoría de productos de cuidado personal, está compuesta, principalmente por productos importados, los cuales constituyen el 70% del mercado, y (b) la preferencia de los consumidores por los productos importados, es mucho más marcada en el segmento de productos de tocador, debido a sus mayores beneficios a precios mucho más económicos. Las grandes empresas del segmento de productos de tocador, en su mayoría son empresas trasnacionales, mientras que en el segmento de productos de limpieza, son las locales quienes comienzan a tener éxito en el mercado. Por este motivo, el presente plan estratégico debe ser considerado como una guía para impulsar a la Industria de Productos de Tocador local y alcanzar la visión planteada para el año 2027, de manera tal, que se posicione a esta industria peruana como la primera dentro de Sudamérica. El análisis realizado propone aplicar estrategias intensivas asociadas en: (a) desarrollo de mercado y producto, y (b) penetración de mercado, así como una estrategia para la integración de empresas conexas de industria, que permitan realizar alianzas con diversos proveedores. Finalmente, se propone una estrategia de integración vertical con el fin de obtener nuevas tecnologías que permita la salida de empresas locales, y que contribuya a posicionar a la Industria de Productos de Tocador Peruana, como referente dentro del ámbito internacional para el año 2027 In recent years, the Toiletry Industry has become a strategic sector for the development of the country's economy. It generates high added value and is driven by a growing international commerce. Even thought, in Peru we would not speak of an active industry, specifically for two reasons: (a) the growth trend in the category of personal care products is mainly composed of imported products, which constitute 70% of the total market and (b) the preference of consumers for imported products is higher than the national toiletries, because there exist the perception that they have more benefits for low cost. As we see the market, we can identify that in the Toiletry Industry the market preferences of the customers are with Multinational Enterprises. Otherwise in the past few years, the household industry, the national products has being gain more market piece. For this reason, to guaranty the national development of the Toiletry Industry, they need a Strategic Plan that should be considered as a guide to achieve the set vision for the 2027: Become the Peruvian Toiletries Products as the first in the Industry from South America. The present analysis proposes to apply intensive strategies related in: (a) market and product development, and (b) market penetration, in order to integrate linked companies to the industry such the alliances with suppliers. Finally, a vertical integration strategy is also proposed in this document, which will help to improve new technologies for local companies in order to support and help to position them with more market share in the Toiletries Industry, in the reference of the next decade Tesis

Published
2017

28. Supplementary Material from Population genetics of immune-related multilocus copy number variation in Native Americans

Author

Zuccherato, Luciana W., Schneider, Silvana, Tarazona-Santos, Eduardo, Hardwick, Robert J., Berg, Douglas E., Bogle, Helen, Gouveia, Mateus H., Machado, Lee R., Moara Machado, Rodrigues-Soares, Fernanda, Soares-Souza, Giordano B., Togni, Diego L., Zamudio, Roxana, Gilman, Robert H., Duarte, Denise, Hollox, Edward J., and Maíra R. Rodrigues

Abstract

Supplementary material, including text, figures and tables.

Published
2017
Full Text
View/download PDF

29. Interethnic Variability in CYP2D6, CYP2C9, and CYP2C19 Genes and Predicted Drug Metabolism Phenotypes Among 6060 Ibero- and Native Americans: RIBEF-CEIBA Consortium Report on Population Pharmacogenomics

Author

Naranjo, María-Eugenia G., primary, Rodrigues-Soares, Fernanda, additional, Peñas-Lledó, Eva M., additional, Tarazona-Santos, Eduardo, additional, Fariñas, Humberto, additional, Rodeiro, Idania, additional, Terán, Enrique, additional, Grazina, Manuela, additional, Moya, Graciela E., additional, López-López, Marisol, additional, Sarmiento, Alba P., additional, Calzadilla, Luis R., additional, Ramírez-Roa, Ronald, additional, Ortiz-López, Rocío, additional, Estévez-Carrizo, Francisco E., additional, Sosa-Macías, Martha, additional, Barrantes, Ramiro, additional, LLerena, Adrián, additional, Fcrreiro, Verónica, additional, Scliar, Marilia O., additional, Gouveia, Mateus H, additional, Borbón, Angélica, additional, Jiménez-Arce, Gerardo, additional, Céspedes-Garro, Carolina, additional, Álvárez, Mayra, additional, Delgado, René, additional, Remirez, Diadelis, additional, Pérez, Bárbaro, additional, Hernández, Francisco, additional, Terán, Santiago, additional, Rojas-Martinez, Augusto, additional, Garza-Ocañas, Lourdes, additional, Pérez-Páramo, Yadira X., additional, Ortega-Vázquez, Alberto, additional, Monroy-Jaramillo, Nancy, additional, Jung-Cook, Helgi, additional, Fricke-Galindo, Ingrid, additional, Galaviz-Hernández, Carlos, additional, Lares-Aseff, Ismael, additional, Lazalde-Ramos, Blanca P., additional, Altamirano Tinoco, Catalina, additional, Zamudio, Roxana, additional, Gilman, Robert H., additional, Cobaleda, Jesús, additional, de Andrés, Fernando, additional, Dorado, Pedro, additional, Naranjo, Eugenia G., additional, and E. Estévez-Carrizo, Francisco, additional

Published
2018
Full Text
View/download PDF

31. Association of caspase-1 polymorphisms with Chagas cardiomyopathy among individuals in Santa Cruz, Bolivia

Author

Fu, Katherine Yih-Jia, primary, Zamudio, Roxana, additional, Henderson-Frost, Jo, additional, Almuedo, Alex, additional, Steinberg, Hannah, additional, Clipman, Steven Joseph, additional, Duran, Gustavo, additional, Marcus, Rachel, additional, Crawford, Thomas, additional, Alyesh, Daniel, additional, Colanzi, Rony, additional, Flores, Jorge, additional, Gilman, Robert Hugh, additional, and Bern, Caryn, additional

Published
2017
Full Text
View/download PDF

32. Population genetics of immune-related multilocus copy number variation in Native Americans

Author

Zuccherato, Luciana W., primary, Schneider, Silvana, additional, Tarazona-Santos, Eduardo, additional, Hardwick, Robert J., additional, Berg, Douglas E., additional, Bogle, Helen, additional, Gouveia, Mateus H., additional, Machado, Lee R., additional, Machado, Moara, additional, Rodrigues-Soares, Fernanda, additional, Soares-Souza, Giordano B., additional, Togni, Diego L., additional, Zamudio, Roxana, additional, Gilman, Robert H., additional, Duarte, Denise, additional, Hollox, Edward J., additional, and Rodrigues, Maíra R., additional

Published
2017
Full Text
View/download PDF

33. Population, Epidemiological, and Functional Genetics of Gastric Cancer Candidate Genes in Peruvians with Predominant Amerindian Ancestry

Author

Zamudio, Roxana, primary, Pereira, Latife, additional, Rocha, Carolina D., additional, Berg, Douglas E., additional, Muniz-Queiroz, Thaís, additional, Sant Anna, Hanaisa P., additional, Cabrera, Lilia, additional, Combe, Juan M., additional, Herrera, Phabiola, additional, Jahuira, Martha H., additional, Leão, Felipe B., additional, Lyon, Fernanda, additional, Prado, William A., additional, Rodrigues, Maíra R., additional, Rodrigues-Soares, Fernanda, additional, Santolalla, Meddly L., additional, Zolini, Camila, additional, Silva, Aristóbolo M., additional, Gilman, Robert H., additional, Tarazona-Santos, Eduardo, additional, and Kehdy, Fernanda S. G., additional

Published
2015
Full Text
View/download PDF

34. Socioeconomic and Nutritional Factors Account for the Association of Gastric Cancer with Amerindian Ancestry in a Latin American Admixed Population

Author

Pereira, Latife, primary, Zamudio, Roxana, additional, Soares-Souza, Giordano, additional, Herrera, Phabiola, additional, Cabrera, Lilia, additional, Hooper, Catherine C., additional, Cok, Jaime, additional, Combe, Juan M., additional, Vargas, Gloria, additional, Prado, William A., additional, Schneider, Silvana, additional, Kehdy, Fernanda, additional, Rodrigues, Maira R., additional, Chanock, Stephen J., additional, Berg, Douglas E., additional, Gilman, Robert H., additional, and Tarazona-Santos, Eduardo, additional

Published
2012
Full Text
View/download PDF

36. Origin and dynamics of admixture in Brazilians and its effect on the pattern of deleterious mutations.

Author

Kehdy, Fernanda S. G., Gouveia, Mateus H., Machado, Moara, Magalhães, Wagner C. S., Horimoto, Andrea R., Horta, Bernardo L., Moreira, Rennan G., Leal, Thiago P., Scliar, Marilia O., Soares-Souza, Giordano B., Rodrigues-Soares, Fernanda, Araújo, Gilderlanio S., Zamudio, Roxana, Sant Anna, Hanaisa P., Santos, Hadassa C., Duarte, Nubia E., Fiaccone, Rosemeire L., Figueiredo, Camila A., Silva, Thiago M., and Costa, Gustavo N. O.

Subjects
POPULATION genetics, HUMAN genome, BAYESIAN analysis, GENOTYPES, DIASPORA
Abstract

While South Americans are underrepresented in human genomic diversity studies, Brazil has been a classical model for population genetics studies on admixture. We present the results of the EPIGEN Brazil Initiative, the most comprehensive up-to-date genomic analysis of any Latin-American population. A population-based genomewide analysis of 6,487 individuals was performed in the context of worldwide genomic diversity to elucidate how ancestry, kinship, and inbreeding interact in three populations with different histories from the Northeast (African ancestry: 50%), Southeast, and South (both with European ancestry >70%) of Brazil. We showed that ancestry-positive assortative mating permeated Brazilian history. We traced European ancestry in the Southeast/South to a wider European/Middle Eastern region with respect to the Northeast, where ancestry seems restricted to Iberia. By developing an approximate Bayesian computation framework, we infer more recent European immigration to the Southeast/South than to the Northeast. Also, the observed low Native-American ancestry (6-8%) was mostly introduced in different regions of Brazil soon after the European Conquest. We broadened our understanding of the African diaspora, the major destination of which was Brazil, by revealing that Brazilians display two within-Africa ancestry components: one associated with non-Bantu/western Africans (more evident in the Northeast and African Americans) and one associated with Bantu/eastern Africans (more present in the Southeast/ South). Furthermore, the whole-genome analysis of 30 individuals (42-fold deep coverage) shows that continental admixture rather than local post-Columbian history is the main and complex determinant of the individual amount of deleterious genotypes. [ABSTRACT FROM AUTHOR]

Published
2015
Full Text
View/download PDF

37. The New Klebsiellapneumoniae ST152 Variants with Hypermucoviscous Phenotype Isolated from Renal Transplant Recipients with Asymptomatic Bacteriuria—Genetic Characteristics by WGS.

Author

Wysocka, Magdalena, Zamudio, Roxana, Oggioni, Marco R, Gołębiewska, Justyna, Dudziak, Aleksandra, and Krawczyk, Beata

Subjects
*KIDNEY transplantation, *KLEBSIELLA infections, *URINARY tract infections, *BACTERIURIA, *HORIZONTAL gene transfer, *SINGLE nucleotide polymorphisms, *PLASMID genetics, *PLASMIDS
Abstract

Klebsiella pneumoniae (Kp) is one of the most important etiological factors of urinary tract infections in renal transplant (RTx) recipients. We described the antimicrobial susceptibility phenotypes and genomic features of two hypermucoviscous (HM) Kp isolates recovered from RTx recipients with asymptomatic bacteriuria (ABU). Using whole genome sequencing (WGS) data, we showed that the strains belong to the ST152 lineage with the KL149 capsular serotype, but without rmpA/magA genes, which is typical for HM+ hypervirulent Kp. These new strains carried virulence-associated genes that predispose for urinary tract infections (UTIs). Likewise, both strains carried the ecp gene encoding pilus common for extended-spectrum β-lactamase (ESBL) Escherichia coli. Although the two ST152 isolates were closely related and differed by only nine single nucleotide polymorphisms (SNPs) in their chromosomes, they had different plasmid compositions and chromosomal elements, with isolate KP28872 carrying an ESBL plasmid and an integrative conjugative element. These two isolates are an example of the high plasticity of the K. pneumoniae accessory genome. The identification of patients with ABU matched with the correct epidemiological profiling of isolates could facilitate interventions to prevent or rapidly treat K. pneumoniae infections. [ABSTRACT FROM AUTHOR]

Published
2020
Full Text
View/download PDF

38. Genomic Stability of Composite SCC mec ACME and COMER-Like Genetic Elements in Staphylococcus epidermidis Correlates With Rate of Excision.

Author

Almebairik N, Zamudio R, Ironside C, Joshi C, Ralph JD, Roberts AP, Gould IM, Morrissey JA, Hijazi K, and Oggioni MR

Abstract

The epidemiological success of methicillin-resistant Staphylococcus aureus USA300 has been associated with the presence of two mobile elements, the arginine catabolic mobile element (ACME) and the copper and mercury resistance (COMER) element. These two mobile elements are associated with resistance to copper, which has been related to host fitness and survival within macrophages. Several studies found that ACME is more prevalent, and exhibits greater diversity, in Staphylococcus epidermidis while COMER has not been identified in S. epidermidis or any other staphylococcal species. We aimed in this study to evaluate the presence and diversity of ACME and COMER-like elements in our S. epidermidis clinical isolates. The genomes of 58 S. epidermidis clinical isolates, collected between 2009 and 2018 in a Scottish hospital, were sequenced. A core-genome phylogenetic tree and genome based MLST typing showed that more than half of the isolates belong to the clinically predominant sequence type2 (ST2) and these isolates have been found to split into two lineages within the phylogenetic tree. Analysis showed the presence of SCC mec in the majority of isolates. Comparative analysis identified a cluster of ACME-positive isolates with most of them belonging to ST48. ACME showed high variation even between isolates of the same ACME type and ST. COMER-like elements have been identified in one of the two major hospital adapted drug resistant ST2 lineages; and showed high stability. This difference in stability at the genomic level correlates well with the up to one hundred times higher excision frequency found for the SCC mec elements in ACME-containing isolates compared to COMER-like element containing isolates. ACME/COMER-like element positive isolates did not show a significant phenotype of decreased copper susceptibility, while resistance to mercury was over-represented in COMER-like element positive isolates. To the best of our knowledge, this is the first molecular characterization of COMER-like elements in S. epidermidis isolates. The presence of the COMER-like elements is the most prominent accessory genome feature of these successful lineages suggesting that this chromosomal island contributes to the success and wide clinical distribution of ST2 S. epidermidis ., (Copyright © 2020 Almebairik, Zamudio, Ironside, Joshi, Ralph, Roberts, Gould, Morrissey, Hijazi and Oggioni.)

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results