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21 results on '"Zamora, Paula F."'

5. Adaptation and genomic erosion in fragmented Pseudomonas aeruginosa populations in the sinuses of people with cystic fibrosis

Author

Armbruster, Catherine R., primary, Marshall, Christopher W., additional, Garber, Arkadiy I., additional, Melvin, Jeffrey A., additional, Zemke, Anna C., additional, Moore, John, additional, Zamora, Paula F., additional, Li, Kelvin, additional, Fritz, Ian L., additional, Manko, Christopher D., additional, Weaver, Madison L., additional, Gaston, Jordan R., additional, Morris, Alison, additional, Methé, Barbara, additional, DePas, William H., additional, Lee, Stella E., additional, Cooper, Vaughn S., additional, and Bomberger, Jennifer M., additional

Published
2021
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6. Genomic and functional characterization of Pseudomonas aeruginosa-targeting bacteriophages isolated from hospital wastewater

Author

Nordstrom, Hayley R., primary, Evans, Daniel R., additional, Finney, Amanda G., additional, Westbrook, Kevin J., additional, Zamora, Paula F., additional, Iovleva, Alina, additional, Yassin, Mohamed H., additional, Bomberger, Jennifer M., additional, Shields, Ryan K., additional, Doi, Yohei, additional, and Van Tyne, Daria, additional

Published
2021
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8. A modified lysosomal organelle mediates nonlytic egress of reovirus

Author

U.S. Public Health Service, Fernández de Castro, Isabel, Tenorio, Raquel, Ortega-González, Paula, Knowlton, Jonathan J., Zamora, Paula F., Lee, Christopher H., Fernández, José J., Dermody, Terence S., Risco, Cristina, U.S. Public Health Service, Fernández de Castro, Isabel, Tenorio, Raquel, Ortega-González, Paula, Knowlton, Jonathan J., Zamora, Paula F., Lee, Christopher H., Fernández, José J., Dermody, Terence S., and Risco, Cristina

Abstract

Mammalian orthoreoviruses (reoviruses) are nonenveloped viruses that replicate in cytoplasmic membranous organelles called viral inclusions (VIs) where progeny virions are assembled. To better understand cellular routes of nonlytic reovirus exit, we imaged sites of virus egress in infected, nonpolarized human brain microvascular endothelial cells (HBMECs) and observed one or two distinct egress zones per cell at the basal surface. Transmission electron microscopy and 3D electron tomography (ET) of the egress zones revealed clusters of virions within membrane-bound structures, which we term membranous carriers (MCs), approaching and fusing with the plasma membrane. These virion-containing MCs emerged from larger, LAMP-1–positive membranous organelles that are morphologically compatible with lysosomes. We call these structures sorting organelles (SOs). Reovirus infection induces an increase in the number and size of lysosomes and modifies the pH of these organelles from ∼4.5–5 to ∼6.1 after recruitment to VIs and before incorporation of virions. ET of VI–SO–MC interfaces demonstrated that these compartments are connected by membrane-fusion points, through which mature virions are transported. Collectively, our results show that reovirus uses a previously undescribed, membrane-engaged, nonlytic egress mechanism and highlights a potential new target for therapeutic intervention.

Published
2020

9. A modified lysosomal organelle mediates nonlytic egress of reovirus

Author

National Institutes of Health (US), Fernández de Castro, Isabel, Tenorio, Raquel, Ortega-González, Paula, Knowlton, Jonathan J, Zamora, Paula F, Lee, Christopher H, Fernández, José J, Dermody, Terence S, Risco, Cristina, National Institutes of Health (US), Fernández de Castro, Isabel, Tenorio, Raquel, Ortega-González, Paula, Knowlton, Jonathan J, Zamora, Paula F, Lee, Christopher H, Fernández, José J, Dermody, Terence S, and Risco, Cristina

Abstract

Mammalian orthoreoviruses (reoviruses) are nonenveloped viruses that replicate in cytoplasmic membranous organelles called viral inclusions (VIs) where progeny virions are assembled. To better understand cellular routes of nonlytic reovirus exit, we imaged sites of virus egress in infected, nonpolarized human brain microvascular endothelial cells (HBMECs) and observed one or two distinct egress zones per cell at the basal surface. Transmission electron microscopy and 3D electron tomography (ET) of the egress zones revealed clusters of virions within membrane-bound structures, which we term membranous carriers (MCs), approaching and fusing with the plasma membrane. These virion-containing MCs emerged from larger, LAMP-1-positive membranous organelles that are morphologically compatible with lysosomes. We call these structures sorting organelles (SOs). Reovirus infection induces an increase in the number and size of lysosomes and modifies the pH of these organelles from ∼4.5-5 to ∼6.1 after recruitment to VIs and before incorporation of virions. ET of VI-SO-MC interfaces demonstrated that these compartments are connected by membrane-fusion points, through which mature virions are transported. Collectively, our results show that reovirus uses a previously undescribed, membrane-engaged, nonlytic egress mechanism and highlights a potential new target for therapeutic intervention.

Published
2020

10. Evolution and adaptation ofPseudomonas aeruginosain the paranasal sinuses of people with cystic fibrosis

Author

Armbruster, Catherine R., primary, Marshall, Christopher W., additional, Melvin, Jeffrey A., additional, Zemke, Anna C., additional, Garber, Arkadiy I., additional, Moore, John, additional, Li, Kelvin, additional, Zamora, Paula F., additional, Fritz, Ian L., additional, Manko, Christopher, additional, Weaver, Madison, additional, Gaston, Jordan, additional, Morris, Alison, additional, Methé, Barbara, additional, Lee, Stella E., additional, Cooper, Vaughn S., additional, and Bomberger, Jennifer M., additional

Published
2020
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11. A modified lysosomal organelle mediates nonlytic egress of reovirus

Author

Fernández de Castro, Isabel, primary, Tenorio, Raquel, additional, Ortega-González, Paula, additional, Knowlton, Jonathan J., additional, Zamora, Paula F., additional, Lee, Christopher H., additional, Fernández, José J., additional, Dermody, Terence S., additional, and Risco, Cristina, additional

Published
2020
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19. Adaptation and genomic erosion in fragmented Pseudomonas aeruginosapopulations in the sinuses of people with cystic fibrosis

Author

Armbruster, Catherine R., Marshall, Christopher W., Garber, Arkadiy I., Melvin, Jeffrey A., Zemke, Anna C., Moore, John, Zamora, Paula F., Li, Kelvin, Fritz, Ian L., Manko, Christopher D., Weaver, Madison L., Gaston, Jordan R., Morris, Alison, Methé, Barbara, DePas, William H., Lee, Stella E., Cooper, Vaughn S., and Bomberger, Jennifer M.

Abstract

Pseudomonas aeruginosanotoriously adapts to the airways of people with cystic fibrosis (CF), yet how infection-site biogeography and associated evolutionary processes vary as lifelong infections progress remains unclear. Here we test the hypothesis that early adaptations promoting aggregation influence evolutionary-genetic trajectories by examining longitudinal P. aeruginosafrom the sinuses of six adults with CF.Highly host-adapted lineages harbored mutator genotypes displaying signatures of early genome degradation associated with recent host restriction. Using an advanced imaging technique (MiPACT-HCR [microbial identification after passive clarity technique]), we find population structure tracks with genome degradation, with the most host-adapted, genome-degraded P. aeruginosa(the mutators) residing in small, sparse aggregates. We propose that following initial adaptive evolution in larger populations under strong selection for aggregation, P. aeruginosapersists in small, fragmented populations that experience stronger effects of genetic drift. These conditions enrich for mutators and promote degenerative genome evolution. Our findings underscore the importance of infection-site biogeography to pathogen evolution.

Published
2021
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20. A modified lysosomal organelle mediates nonlytic egress of reovirus.

Author

de Castro, Isabel Fernández, Tenorio, Raquel, Ortega-González, Paula, Knowlton, Jonathan J., Zamora, Paula F., Lee, Christopher H., Fernández, José J., Dermody, Terence S., and Risco, Cristina

Subjects
*TRANSMISSION electron microscopy, *LYSOSOMES, *VIRION, *ENDOTHELIAL cells, *ORGANELLES, *REOVIRUSES, *CELL compartmentation
Abstract

Mammalian orthoreoviruses (reoviruses) are nonenveloped viruses that replicate in cytoplasmic membranous organelles called viral inclusions (VIs) where progeny virions are assembled. To better understand cellular routes of nonlytic reovirus exit, we imaged sites of virus egress in infected, nonpolarized human brain microvascular endothelial cells (HBMECs) and observed one or two distinct egress zones per cell at the basal surface. Transmission electron microscopy and 3D electron tomography (ET) of the egress zones revealed clusters of virions within membrane-bound structures, which we term membranous carriers (MCs), approaching and fusing with the plasma membrane. These virion-containing MCs emerged from larger, LAMP-1–positive membranous organelles that are morphologically compatible with lysosomes. We call these structures sorting organelles (SOs). Reovirus infection induces an increase in the number and size of lysosomes and modifies the pH of these organelles from ∼4.5–5 to ∼6.1 after recruitment to VIs and before incorporation of virions. ET of VI–SO–MC interfaces demonstrated that these compartments are connected by membrane-fusion points, through which mature virions are transported. Collectively, our results show that reovirus uses a previously undescribed, membrane-engaged, nonlytic egress mechanism and highlights a potential new target for therapeutic intervention. [ABSTRACT FROM AUTHOR]

Published
2020
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21. Lytic bacteriophages interact with respiratory epithelial cells and induce the secretion of antiviral and proinflammatory cytokines.

Author

Zamora PF, Reidy TG, Armbruster CR, Sun M, Van Tyne D, Turner PE, Koff JL, and Bomberger JM

Abstract

Phage therapy is a therapeutic approach to treat multidrug resistant infections that employs lytic bacteriophages (phages) to eliminate bacteria. Despite the abundant evidence for its success as an antimicrobial in Eastern Europe, there is scarce data regarding its effects on the human host. Here, we aimed to understand how lytic phages interact with cells of the airway epithelium, the tissue site that is colonized by bacterial biofilms in numerous chronic respiratory disorders. We determined that interactions between phages and epithelial cells depend on specific phage properties as well as physiochemical features of the microenvironment. Although poor at internalizing phages, the airway epithelium responds to phage exposure by changing its transcriptional profile and secreting antiviral and proinflammatory cytokines that correlate with specific phage families. Overall, our findings indicate that mammalian responses to phages are heterogenous and could potentially alter the way that respiratory local defenses aid in bacterial clearance during phage therapy. Thus, besides phage receptor specificity in a particular bacterial isolate, the criteria to select lytic phages for therapy should be expanded to include mammalian cell responses.

Published
2024
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