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In response to evolving climatic conditions, plants frequently confront multiple abiotic stresses, necessitating robust adaptive mechanisms. This study focuses on the responses of Selenicereus undatus L. to both individual stresses (cadmium; Cd, salt; S, and drought; D) and their combined applications, with an emphasis on evaluating the mitigating effects of (M) melatonin. Through transcriptome analysis, this study identifies significant gene expression changes and regulatory network activations. The results show that stress decreases pitaya growth rates by 30%, reduces stem and cladode development by 40%, and increases Cd uptake under single and combined stresses by 50% and 70%, respectively. Under stress conditions, enhanced activities of H2O2, POD, CAT, APX, and SOD and elevated proline content indicate strong antioxidant defenses. We identified 141 common DEGs related to stress tolerance, most of which were related to AtCBP, ALA, and CBP pathways. Interestingly, the production of genes related to signal transduction and hormones, including abscisic acid and auxin, was also significantly induced. Several calcium-dependent protein kinase genes were regulated during M and stress treatments. Functional enrichment analysis showed that most of the DEGs were enriched during metabolism, MAPK signaling, and photosynthesis. In addition, weighted gene co-expression network analysis (WGCNA) identified critical transcription factors (WRKYs, MYBs, bZIPs, bHLHs, and NACs) associated with antioxidant activities, particularly within the salmon module. This study provides morpho-physiological and transcriptome insights into pitaya's stress responses and suggests molecular breeding techniques with which to enhance plant resistance. [ABSTRACT FROM AUTHOR]

Under changing climatic conditions, plants are simultaneously facing conflicting stresses in nature. Plants can sense different stresses, induce systematic ROS signals, and regulate transcriptomic, hormonal, and stomatal responses. We performed transcriptome analysis to reveal the integrative stress response regulatory mechanism underlying heavy metal stress alone or in combination with heat and drought conditions in pitaya (dragon fruit). A total of 70 genes were identified from 31,130 transcripts with conserved differential expression. Furthermore, weighted gene co‐expression network analysis (WGCNA) identified trait‐associated modules. By integrating information from three modules and protein–protein interaction (PPI) networks, we identified 10 interconnected genes associated with the multifaceted defense mechanism employed by pitaya against co‐occurring stresses. To further confirm the reliability of the results, we performed a comparative analysis of 350 genes identified by three trait modules and 70 conserved genes exhibiting their dynamic expression under all treatments. Differential expression pattern of genes and comparative analysis, have proven instrumental in identifying ten putative structural genes. These ten genes were annotated as PLAT/LH2, CAT, MLP, HSP, PB1, PLA, NAC, HMA, and CER1 transcription factors involved in antioxidant activity, defense response, MAPK signaling, detoxification of metals and regulating the crosstalk between the complex pathways. Predictive analysis of putative candidate genes, potentially governing single, double, and multifactorial stress response, by several signaling systems and molecular patterns. These findings represent a valuable resource for pitaya breeding programs, offering the potential to develop resilient "super pitaya" plants. [ABSTRACT FROM AUTHOR]

DNA binding proteins with one finger (Dof ) transcription factors are essential for seed development and defence against various biotic and abiotic stresses in plants. Genomic analysis of Dof has not been determined yet in pitaya (Selenicereus undatus). In this study, we have identified 26 Dof gene family members, renamed as HuDof-1 to HuDof-26, and clustered them into seven subfamilies based on conserved motifs, domains, and phylogenetic analysis. The gene pairs of Dof family members were duplicated by segmental duplications that faced purifying selection, as indicated by the Ka/Ks ratio values. Promoter regions of HuDof genes contain many cis-acting elements related to phytohormones including abscisic acid, jasmonic acid, gibberellin, temperature, and light. We exposed pitaya plants to different environmental stresses and examined melatonin’s influence on Dof gene expression levels. Signifcant expression of HuDof-2 and HuDof-6 were observed in different developmental stages of flower buds, flowers, pericarp, and pulp. Pitaya plants were subjected to abiotic stresses, and transcriptome analysis was carried out to identify the role of Dof gene family members. RNA-sequencing data and reverse transcription quantitative PCR-based expression analysis revealed three putative candidate genes (HuDof-1, HuDof-2, and HuDof-8), which might have diverse roles against the abiotic stresses. Our study provides a theoretical foundation for functional analysis through traditional and modern biotechnological tools for pitaya trait improvement. [ABSTRACT FROM AUTHOR]

Hair fescue is an economically destructive, tuft‐forming weed common in wild blueberry fields. The current management of hair fescue is almost completely reliant on pronamide (3,5‐dichloro‐N‐[2‐methylbut‐3‐yn‐2‐yl]benzamide) herbicide with few other products demonstrating effectiveness. Dichlobenil (2,6‐dichlorobenzonitrile) is a group 29 herbicide registered for use in wild blueberry; however, it has seen limited use because of its high product cost of over CAD 1800 ha−1 and limited research on the effect of varying application rates. Treatments (dichlobenil at 4400, 5700, and 7000 g a.i. ha−1, pronamide at 2240 g a.i. ha−1, and a nontreated control) were applied during the 2020 autumn prior to wild blueberry stem emergence in the nonbearing year (2021). Each treatment was replicated five times per field across three fields in central Nova Scotia. Dichlobenil at 4400, 5700, and 7000 g a.i. ha−1 reduced 2021 (nonbearing year) living tuft density by 59%, 71%, and 83%, respectively, and provided residual control in 2022 (bearing year) with densities reduced by 59%, 69%, and 78%, respectively. Comparatively, pronamide reduced 2021 and 2022 living tuft density by 95% and 91%. Reductions in living tuft density resulted in yield increases of 62%, 57%, and 94% in plots treated with dichlobenil at 4400, 5700, and 7000 g a.i. ha−1, respectively, and 73% for plots treated with pronamide. Results demonstrate that dichlobenil is an effective herbicide for managing hair fescue in wild blueberry. The potential for the selection of herbicide‐resistant biotypes to industry standard pronamide could be prolonged with integrated dichlobenil applications. Core Ideas: Hair fescue is a yield‐limiting weed with few effective herbicide options in wild blueberry.Alternative chemicals with a different mode‐of‐action than pronamide are needed to prevent resistant grass selection.Dichlobenil at the highest labeled rate controlled hair fescue and increased wild blueberry yield.Rotating pronamide with dichlobenil should reduce the potential for herbicide‐resistant grass selection. [ABSTRACT FROM AUTHOR]

Together with rice, weeds strive for nutrients and space in farmland, resulting in reduced rice yield and quality. Planting herbicide-resistant rice varieties is one of the effective ways to control weeds. In recent years, a series of breakthroughs have been made to generate herbicide-resistant germplasm, especially the emergence of biotechnological tools such as gene editing, which provides an inherent advantage for the knock-out or knock-in of the desired genes. In order to develop herbicideresistant rice germplasm resources, gene manipulation has been conducted to enhance the herbicide tolerance of rice varieties through the utilization of techniques such as physical and chemical mutagenesis, as well as genome editing. Based on the current research and persisting problems in rice paddy fields, research on the generation of herbicide-resistant rice still needs to explore genetic mechanisms, stacking multiple resistant genes in a single genotype, and transgene-free genome editing using the CRISPR system. Current rapidly developing gene editing technologies can be used to mutate herbicide target genes, enabling targeted genes to maintain their biological functions, and reducing the binding ability of target gene encoded proteins to corresponding herbicides, ultimately resulting in herbicide-resistant crops. In this review article, we have summarized the utilization of conventional and modern approaches to develop herbicide-resistant cultivars in rice as an effective strategy for weed control in paddy fields, and discussed the technology and research directions for creating herbicide-resistant rice in the future. [ABSTRACT FROM AUTHOR]