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Daging sapi dan olahannya merupakan sasaran pemalsuan. Pelaku pemalsuan daging sapi mencampur daging sapi dengan daging spesies hewan lain, sehingga berakibat pada berubahnya status kehalalan daging. Selain itu, kondisi tersebut dapat menjadi penyebab serius pada risiko kesehatan, seperti potensi keracunan, sumber penyakit maupun alergi. Pengawasan yang efektif diperlukan guna menjamin keaslian dan keamanan daging. Metode deteksi secara molekuler yang umum digunakan adalah multiplex-PCR karena efisien dan sensitif. Tujuan penelitian ini untuk mendeteksi sensitivitas multiplex-PCR primer gen 12S rRNA yang spesifik untuk spesies sapi, anjing, babi, dan tikus. Template DNA dibuat masing-masing enam konsentrasi, yaitu 25; 10; 1; 0,1; 0,01; 0,001 ng/ìL untuk proses simplex dan multiplex-PCR. Hasil uji sensitivitas menggunakan metode simplex-PCR menunjukkan bahwa primer gen 12S rRNA dapat mengamplifikasi spesies sapi, anjing, babi, dan tikus dengan akurat hingga konsentrasi 0,001 ng/ìL. Pengujian sensitivitas multiplex-PCR gen 12S rRNA dapat mengamplifikasi spesies sapi, anjing, babi hingga konsentrasi 0,001 ng/ìL dan pada spesies tikus hingga konsentrasi 1 ng/ìL. Metode PCR ini dapat digunakan sebagai solusi alternatif dalam studi halal dan verifikasi label pangan

The objective of this study was to detect three non-halal meat products consisted of dog, pork, and rat species in meatball using novel multiplex-PCR with 12S rRNA gene as target sites. A total of 33 self-made meatballs were used, and they were grouped into eleven types of meatball based on meat species origin contained in the meatballs. Each type consisted of three meatballs. Extraction of genomic DNA from the meatballs was used as a DNA template for simplex-, duplex-, and multiplex-PCR processes. The result of simplex-PCR, duplex-PCR, and multiplex-PCR showed that the 12S rRNA primer gene successfully amplified DNA for each species bovine, dog, pig, and rat, which are respectively indicated by 155, 244, 357, and 491 bp of DNA bands. In addition, multiplex-PCR with 12S rRNA gene primers can be uniquely and accurately used for detection bovine, dog, pig, and rat species on beef meatball in one reaction.

Tujuan penelitian ini adalah untuk untuk mengetahui pengaruh bahan penyusun briket dari kotoran puyuh dan limbah pertanian dengan tambahan perekat sebagai bahan bakar alternatif. Penelitian ini dilaksanakan pada bulan April sampai Juni 2019 di Laboraturium Balai Pelayanan dan Kesehatan Masyarakat Veteriner Boyolali, Laboraturium Universitas Diponegoro, dan di Laboraturium Universitas Boyolali. Materi yang digunakan adalah briket sebanyak 90 buah yang dibuat dengan 3 perlakuan dan 6 kali ulangan. Dalam penelitian ini terdapat 3 perlakuan, dengan bahan kotoran puyuh dan sekam dengan perbandingan 1:1, 2:1, dan 3:1. Parameter yang diteliti yaitu kadar air, kadar abu, lama bakar briket sampai menjadi abu, dan lama untuk mendidihkan 250 ml air. Metode penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan tiga perlakuan dan enam kali ulangan. Masing-masing ulangan terdiri dari 6 briket. Penelitian ini dilakukan dalam 2 tahap yaitu tahap persiapan dan tahap pelaksanaan. Hasil penelitian menunjukan bahwa lama nyala briket sampai menjadi abu dan lama untuk mendidihkan air tidak berpengaruh nyata sedangkan kadar abu dan kadar air berpengaruh nyata. Kesimpulan dari hasil penelitian ini adalah perbandingan sekam dan kotoran puyuh menghasilkan briket terbaik pada perlakuan perbandingan kotoran puyuh : sekam sebesar 22,5 : 7,5 karena mempunyai kadar abu dan kadar terendah.

The 12S rRNA gene is one of unique regions in mitochondrial genome usually used for phylogenetic studies and species identification. The objective of present study was to develop species specific primer s from mitochondrial 12S rRNA gene for identification of dog and rat in beef by using multiplex PCR assay . Three primer pairs of mitochondrial 12S rRNA gene specific for bovine, dog and rat were designed and selected to evaluate their specificity and fidelity. Moreover , a total of twelve DNA samples extracted from meat tissue were also prepared to test those primers using simplex and multiplex PCR. The PCR products were then visualized using 2% of agarose gel under the UV light and three of them were sequenced. In addition, sequence data were analyzed using Clustal Omega software and BLAST. The result showed that simplex PCR assay successfully amplified DNA targets which are respectively indicated by 155 bp (bovine), 244 bp (dog), and 491 bp (rat) of DNA bands. Furthermore, DNA sample sequences were identically similar to reference sequence used in this study. Multiplex and simplex PCR analyses also indicated that these primer pairs specifically amplified DNA target for each species in the samples containing various species. The results suggested that designed primers in this study could be used to identify dog and rat in raw beef containing these species meat. Further experiment should be conducted using meat-processed products and commercial meat products as samples.

Pemanfaatan antibiotik sebagai pemacu pertumbuhan ayam mulai dilarang karena adanya pengaruh merugikan bagi kesehatan manusia. Alternatif lain selain menggunakan antibiotik adalah menggunakan probiotik dan prebiotik. Penelitian tentang probiotik dan prebiotik telah dilakukan dalam beberapa tahun terakhir pada ternak ayam. Penggunaan kombinasi probiotik dan prebiotik yang diberikan secara bersama dapat meningkatkan proses penyerapan nutrien pada usus ayam, sehingga berdampak pada peningkatan performans, dan pada akhirnya meningkatkan kualitas daging. Eksplorasi sumber probiotik dan prebiotik masih perlu dilakukan terutama kombinasi dan kondisi yang optimal berdasarkan berbagai faktor seperti jenis ayam, jenis probiotik, dan jenis prebiotik.

Availability of commercial feeds for chicken force farmers to use them due to the practicality than hand formulated feed. Cannibalism and spread of infectious disease may be occurred by feed without knowing its contents. Therefore, the aim of this study was to determine species contained in commercial feeds for chicken using multiplex-PCR mitochondrial DNA Cytochrome b gene. This study used five commercial feeds for chicken produced by five different companies as samples. They were namely PK1, PK2, PK3, PK4, and PK5. Hand-made meat meals from goat, chicken, bovine, and pig were used as positive controls. Genomic DNA was extracted from those samples, and they were used as DNA template for PCR. The PCR products were then visualized using 2% agarose gels under the UV light. The results showed that some samples were positively containing animal derivatives. The PK1 sample contained chicken, bovine, and pig which are respectively indicated by 227, 274, and 398 bp of DNA bands. Moreover, PK4 and PK5 samples contained bovine species. On the other hand, PK2 and PK3 samples did not contain animal derivatives, especially four species used as markers in this study. It can be concluded that animal derivatives have been detected in three commercial feeds for chicken using the multiplex-PCR technique. This study recommended avoiding a commercial feed for chicken since it contained chicken derivatives to prevent cannibalism.

The objective of the study was to determine the effects of feeding Lactobacillus species (Lactobacillus sp.) and inulin derived from dahlia tuber powder on antioxidant activity, calcium mass, and protein mass of crossbred kampong chicken meat. A total of 168 birds of 21 days old crossbred kampong chickens were randomly allocated into 6 treatments with four replications per treatment. The present experiment was assigned in a completely randomized design with 2 x 3 factorial scheme. The first factor was levels of dahlia tuber powder, namely 0.8% (A1) and 1.2% (A2), and the second factor was levels of Lactobacillus sp., namely none (B0), 1.2 mL (108 cfu/mL/B1) and 2.4 mL (108 cfu/mL/B2). The parameters measured were antioxidant activity, meat calcium and protein mass. Data were subjected to analysis of variance and followed by Duncan multiple range test (P

The authenticity of meat bone meal (MBM) in animal feed is needed to be considered due to inappropriate feed ingredients for certain types of livestock may cause various diseases. This work was aimed to identify animal species in MBM using multiplex PCR assay with 12S rRNA gene as a target region. Total of eight DNA samples were extracted from three species (chicken, bovine, and porcine) which are used as positive controls and three MBM samples. The MBM samples were collected from three different importers. Additionally, a multiplex PCR assay has been performed to identify animal species in MBM. Multiplex PCR of 12S rRNA gene was designed to detect bovine, chicken, and porcine in MBM samples. The PCR products was visualized using 2% agarose gels under the UV light. The results showed that multiplex-PCR of 12S rRNA gene was able to identify bovine which is indicated by 155 bp of DNA band. Chicken and porcine were not found in MBM samples. Meat bone meal samples used in this study might be used to be a feedstuff for poultry, porcine, and other non-ruminants. In conclusion, Multiplex-PCR using mt-DNA 12S rRNA was effective and accurate technique to identify species contained in MBM samples.

Tujuan penelitian ini adalah untuk mengetahui pengaruh penggunaan lidah buaya ( Aloe vera ) dalam ransum terhadap produktivitas ayam broiler pejantan. Penelitian menggunakan Rancangan Acak Lengkap pola searah dilanjutkan dengan uji lanjut Duncans Multiple Range Test. Materi yang digunakan adalah 100 ekor DOC jantan yang dipelihara dalam 20 petak kandang yang terdiri dari 5 ekor ayam per unit percobaan. Perlakuan dalam penelitian ini yaitu T0 = Ransum kontrol, T1 = ransum komersial dan lidah buaya 0,75 %, T2 = ransum komersial dan lidah buaya 1,5%, T3 = ransum komersial dan lidah buaya 2%. Peubah yang diteliti yaitu bobot badan, konsumsi ransum, dan Feed convertion ratio . Hasil penelitian menunjukkan bahwa pemberian lidah buaya T3 sebanyak 2% dalam ransum berpengaruh terhadap pertambahan bobot badan harian dan persentase karkas tetapi tidak berpengaruh meningkatkan konsumsi ransum dibandingkan dengan kontrol. Sedangkan konversi pakan (FCR) lebih baik karena nilainya lebih rendah dibandingkan dengan control.