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6 results on '"Zahnow, Christian"'

1. Microbial High Cell Density Fermentations in a Stirred Single-Use Bioreactor

Author

Dreher, Thomas, Walcarius, Bart, Husemann, Ute, Klingenberg, Franziska, Zahnow, Christian, Adams, Thorsten, de Wilde, Davy, Casteels, Peter, Greller, Gerhard, Scheper, T., Series editor, Belkin, Shimshon, Series editor, Doran, Pauline M, Series editor, Endo, Isao, Series editor, Gu, Man Bock, Series editor, Hu, Wei Shou, Series editor, Mattiasson, Bo, Series editor, Nielsen, Jens, Series editor, Stephanopoulos, Gregory N., Series editor, Ulber, Roland, Series editor, Zeng, An-Ping, Series editor, Zhong, Jian-Jiang, Series editor, Zhou, Weichang, Series editor, Eibl, Dieter, editor, and Eibl, Regine, editor

Published
2014
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3. Standardized qualification of stirred bioreactors for microbial biopharmaceutical production processes

Author

Schirmer, Cedric, Blaschczok, Katharina, Husemann, Ute, Leupold, Marco, Zahnow, Christian, Rupprecht, Jens, Glöckler, Rainer, Greller, Gerhard, Pörtner, Ralf, Eibl-Schindler, Regine, Eibl, Dieter, Schirmer, Cedric, Blaschczok, Katharina, Husemann, Ute, Leupold, Marco, Zahnow, Christian, Rupprecht, Jens, Glöckler, Rainer, Greller, Gerhard, Pörtner, Ralf, Eibl-Schindler, Regine, and Eibl, Dieter

Abstract

An in 2016 published DECHEMA guideline concerning process engineering characterization and a new Escherichia coli model process were utilized for the qualification of two geometrically similar stirred stainless steel bioreactors (30 L and 100 L working volume). The achieved results demonstrate that performing an additional biological model process is a valuable complement to the process engineering characterization. Optical densities of 27 (100 L) and 39 (30 L) were reached in the batch cultivation process.

Published
2017

6. High Cell Density Escherichia coli Cultivation in Different Single-Use Bioreactor Systems.

Author

Dreher, Thomas, Husemann, Ute, Zahnow, Christian, de Wilde, Davy, Adams, Thorsten, and Greller, Gerhard

Abstract

During the last decades single-use bioreactors have become widely accepted in the biopharmaceutical industry. Single-use technologies bring many advantages over conventional solutions, such as a reduced investment and operational cost as well as an optimized time-to-market. So far, this type of bioreactor is mainly used for cell culture applications. Results for microbial fermentations are rarely found, commonly due to limited oxygen transfer rates. The aim of this study was to establish a high cell density fermentation process for a recombinant Escherichia coli considering a rocking motion type and an adapted stirred 50-L single-use bioreactor. By using the design space approach an OTRmax/OURmax model was established. The feeding strategies were optimized and verified based on a model for both single-use systems to achieve high cell densities. In both single-use bioreactors a recombinant protein was successfully expressed. [ABSTRACT FROM AUTHOR]

Published
2013
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