Search

Your search keyword '"Zago, Marco Antônio"' showing total 40 results
Start Over Author "Zago, Marco Antônio"
40 results on '"Zago, Marco Antônio"'

2. Shotgun Sequencing of the Human Transcriptome with ORF Expressed Sequence Tags

Author

Neto, Emmanuel Dias, Correa, Ricardo Garcia, Verjovski-Almeida, Sergio, Nagai, Maria Aparecida, da Silva,, Wilson, Zago, Marco Antonio, Bordin, Silvana, Costa, Fernando Ferreira, Goldman, Gustavo Henrique, Carvalho, Alex F., Matsukuma, Adriana, Baia, Gilson S., Simpson, David H., Brunstein, Adriana, Bucher, Philipp, Jongeneel, C. Victor, O'Hare, Michael J., Soares, Fernando, Brentani, Ricardo R., and de Souza, Sandro J.

Published
2000

3. DSP30 enhances the immunosuppressive properties of mesenchymal stromal cells and protects their suppressive potential from lipopolysaccharide effects: A potential role of adenosine

Author

Sangiorgi, Bruno, De Freitas, Helder Teixeira, Schiavinato, Josiane Lilian Dos Santos, Leão, Vitor, Haddad, Rodrigo, Orellana, Maristela Delgado, Faça, Vitor Marcel, Ferreira, Germano Aguiar, Covas, Dimas Tadeu, Zago, Marco Antônio, and Panepucci, Rodrigo Alexandre

Published
2016
Full Text
View/download PDF

5. High-content screen in human pluripotent cells identifies miRNA-regulated pathways controlling pluripotency and differentiation

Author

de Souza Lima, Ildercílio Mota, Schiavinato, Josiane Lilian dos Santos, Paulino Leite, Sarah Blima, Sastre, Danuta, Bezerra, Hudson Lenormando de Oliveira, Sangiorgi, Bruno, Corveloni, Amanda Cristina, Thomé, Carolina Hassibe, Faça, Vitor Marcel, Covas, Dimas Tadeu, Zago, Marco Antônio, Giacca, Mauro, Mano, Miguel, and Panepucci, Rodrigo Alexandre

Published
2019
Full Text
View/download PDF

14. Expression of cancer/testis antigens MAGE-A, MAGE-C1, GAGE and CTAG1B in benign and malignant thyroid diseases

Author

Melo, Daniel Hardy, Mamede, Rui Celso Martins, Neder, Luciano, Silva, Wilson Araújo, Barros-Filho, Mateus Camargo, Kowalski, Luiz Paulo, Pinto, Clóvis Antonio Lopes, Zago, Marco Antônio, Figueiredo, David Livingstone Alves, and Jungbluth, Achim A.

Subjects
endocrine system, biological tumor markers, thyroid neoplasms, thyroid nodule, immunohistochemistry, Articles
Abstract

Despite considerable advances in the understanding of thyroid gland biology, correctly diagnosing thyroid nodules and treating high-grade thyroid carcinoma remains challenging. Cancer/testis (CT) antigens have emerged as potential diagnostic tools as well as targets of potential cancer vaccinations. In the present study, a total of 117 patients who underwent surgical therapy for thyroid disease were available for analysis. The expression levels of melanoma-associated antigen (MAGE) A, MAGE-C1/CT7, cancer/testis antigen 1B (CTAG1B) and G antigen (GAGE) were analyzed by immunohistochemistry. None of the CT antigens were expressed in the normal thyroid or goiter. In papillary and follicular carcinoma, MAGE-A was present in 8.1% of cases, GAGE in 10.8% and CT/7MAGE-C1 and CTAG1B in 2.7% each. In medullary carcinoma, CT antigen expression was as follows: MAGE-A in 42.9% of patients; MAGE-C1/CT7 in 46.5%; GAGE in 92.9%; and CTAG1B in 3.6%. A statistically significant association was observed between the expression of G MAGE-C1/CT7 and patient gender as well as patient clinical stage (P=0.029 and 0.031, respectively). In poorly differentiated and anaplastic carcinoma cases, CT antigen expression was as follows: MAGE-A in 61.8% of cases; MAGE-C1 in 57.1%; GAGE in 66.7%; and CTAG1B in 14.4%. There was a statistically significant association between expression of GAGE and gender (P=0.043). However, there was no association between CT antigen expression and patient survival in any of the tumor entities analyzed. The current study identified a distinct expression pattern of CT antigens in malignant thyroid tumors indicating that CT antigens have the potential to outperform existing thyroid cancer biomarkers. The prevalence of CT antigens in high-grade carcinomas suggests that they serve an important biological role within malignant tumors.

Published
2017

16. A High-Content Screening Approach to Identify MicroRNAs Against Head and Neck Cancer Cell Survival and EMT in an Inflammatory Microenvironment

Author

Sangiorgi, Bruno, primary, de Souza, Felipe Canto, additional, Mota de Souza Lima, Ildercílio, additional, dos Santos Schiavinato, Josiane Lilian, additional, Corveloni, Amanda Cristina, additional, Thomé, Carolina Hassibe, additional, Araújo Silva, Wilson, additional, Faça, Vitor Marcel, additional, Covas, Dimas Tadeu, additional, Zago, Marco Antônio, additional, and Panepucci, Rodrigo Alexandre, additional

Published
2019
Full Text
View/download PDF

17. Host defense and inflammatory gene polymorphisms are associated with outcomes after HLA-identical sibling bone marrow transplantation: Presented in part at the Annual Meeting of the American Society of Hematology, Orlando, FL, December 7-11, 2001.

Author

Rocha, Vanderson, Franco, Rendrik F., Porcher, Raphaël, Bittencourt, Henrique, Silva, Wilson A., Jr, Latouche, Aurelien, Devergie, Agnès, Espérou, Hélène, Ribaud, Patricia, Socié, Gérard, Zago, Marco Antonio, and Gluckman, Eliane

Published
2002
Full Text
View/download PDF

21. Expression of eight genes of nuclear factor-kappa B pathway in multiple myeloma using bone marrow aspirates obtained at diagnosis

Author

Sampaio Almeida, Manuella S., Vettore, André L., Yamamoto, M., Chauffaille, Maria de Lourdes L.F., Zago, Marco Antônio, and Colleoni, Gisele W. B.

Subjects
musculoskeletal diseases, Multiple myeloma, 57 - Biología, Prognosis
Abstract

Purpose: To evaluate the expression of NF- κB pathway genes in total bone marrow samples obtained from MM at diagnosis using real-time quantitative PCR and to evaluate its possible correlation with disease clinical features and survival. Material and methods: Expression of eight genes related to NF-κB pathway (NFKB1, IKB, RANK, RANKL, OPG, IL6, VCAM1 and ICAM1) were studied in 53 bone marrow samples from newly diagnosed MM patients and in seven normal controls, using the Taqman system. Genes were considered overexpressed when tumor expression level was at least four times higher than that observed in normal samples. Results: The percentages of overexpression of the eight genes were: NFKB1 0%, IKB 22.6%, RANK 15.1%, RANKL 31.3%, OPG 7.5%, IL6 39.6%, VCAM1 10% and ICAM1 26%. We found association between IL6 expression level and International Staging System (ISS) (p=0.01), meaning that MM patients with high ISS scores have more chance of overexpression of IL6. The mean value of ICAM1 relative expression was also associated with the ISS score (p=0.02). Regarding OS, cases with IL6 overexpression present worse evolution than cases with IL6 normal expression (p=0.04). Conclusion: We demonstrated that total bone marrow aspirates can be used as a source of material for gene expression studies in MM. In this context, we confirmed that IL6 overexpression was significantly associated with worse survival and we described that it is associated with high ISS scores. Also, ICAM1 was overexpressed in 26% of cases and its level was associated with ISS scores.

Published
2009

22. A pesquisa clínica no Brasil

Author

Zago, Marco Antônio

Subjects
Scientific and technological development, Agenda de pesquisa, Research agenda, Desenvolvimento científico e tecnológico, Pesquisa clínica, Clinical research
Abstract

O fortalecimento da pesquisa clínica no Brasil implica a criação, expansão ou consolidação de novos centros, em especial na recuperação dos hospitais universitários e do fortalecimento do vínculo entre ensino e pesquisa. A interação entre pesquisa biomédica e básica é indispensável para geração de conhecimento nacional em questões específicas e para estruturar o setor produtivo de tecnologia ligada à saúde. As mudanças do perfil demográfico e epidemiológico do país trouxeram para a lista de prioridades de pesquisa em saúde doenças cardiovasculares, neoplasias, doenças mentais, doenças associadas a urbanização, acidentes e violência, e doenças do envelhecimento. O Brasil ainda apresenta endemias importantes no seu quadro epidemiológico: dengue, malária, esquistossomose, hanseníase e hepatites virais, entre outras. A consolidação da pesquisa em biologia celular e molecular aplicada à medicina é indispensável para que a prática médica no país se mantenha atualizada e competitiva, e para permitir interações com o setor produtivo e implantação de um parque biotecnológico nacional. Strengthening clinical research in Brazil involves the creation, expansion, or consolidation of new centers, and especially the recovery of university hospitals and a stronger link between teaching and research. Interaction between biomedical and basic research is indispensable to generate national knowledge on specific issues and to structure the health technology production sector. Changes in the country's demographic and epidemiological profile have brought cardiovascular diseases, neoplasms, psychiatric diseases, diseases associated with urbanization, accidents/violence, and diseases of ageing to the list of health research priorities. Brazil still displays important endemics in its epidemiological profile: dengue, malaria, schistosomiasis, Hansen's disease, and viral hepatites, among others. Consolidation of research in cellular and molecular biology applied to medicine is indispensable for medical practice in the country to remain up-to-date and competitive, as well as to allow interactions with the productive sector and the implementation of a national biotechnology complex.

Published
2004

24. Expression of cancer‐testis antigens MAGE‐A4 and MAGE‐C1 in oral squamous cell carcinoma

Author

Montoro, José Raphael de Moura Campos, primary, Mamede, Rui Celso Martins, additional, Neder Serafini, Luciano, additional, Saggioro, Fabiano Pinto, additional, Figueiredo, David Livingstone Alves, additional, Silva, Wilson Araújo da, additional, Jungbluth, Achim A., additional, Spagnoli, Giulio Cesare, additional, and Zago, Marco Antônio, additional

Published
2011
Full Text
View/download PDF

38. DSP30 enhances the immunosuppressive properties of mesenchymal stromal cells and protects their suppressive potential from lipopolysaccharide effects: A potential role of adenosine.

Author

FAÇA, VITOR MARCEL, FERREIRA, GERMANO AGUIAR, SANGIORGI, BRUNO, SANTOS SCHIAVINATO, JOSIANE LILIAN DOS, LEÃO, VITOR, ZAGO, MARCO ANTÔNIO, PANEPUCCI, RODRIGO ALEXANDRE, DE FREITAS, HELDER TEIXEIRA, ORELLANA, MARISTELA DELGADO, COVAS, DIMAS TADEU, and HADDAD, RODRIGO

Subjects
*MESENCHYMAL stem cells, *IMMUNOSUPPRESSION, *TOLL-like receptors, *PHYSIOLOGY
Abstract

Multipotent mesenchymal stromal cells (MSC) are imbued with an immunosuppressive phenotype that extends to several immune system cells. In this study, we evaluated how distinct Toll-like receptor (TLR) agonists impact immunosuppressive properties of bone marrow (BM)-MSC and explored the potential mechanisms involved. We show that TLR4 stimulation by lipopolysaccharide (LPS) restricted the ability of MSC to suppress the proliferation of T lymphocytes, increasing the gene expression of interleukin (IL)-1β and IL-6. In contrast, stimulation of TLR9 by DSP30 induced proliferation and the suppressive potential of BM-MSC, coinciding with reducing tumor necrosis factor (TNF)-α expression, increased expression of transforming growth factor (TGF)-β1, increased percentages of BM-MSC double positive for the ectonucleotidases CD39+CD73+ and adenosine levels. Importantly, following simultaneous stimulation with LPS and DSP30, BM-MSC's ability to suppress T lymphocyte proliferation was comparable with that of non-stimulated BM-MSC levels. Moreover, stimulation of BM-MSC with LPS reduced significantly the gene expression levels, on co-cultured T lymphocyte, of IL-10 and interferon (IFN)γ, a cytokine with potential to enhance the immunosuppression mediated by MSC and ameliorate the clinical outcome of patients with graft-versus-host disease (GVHD). Altogether, our findings reiterate the harmful effects of LPS on MSC immunosuppression, besides indicating that DSP30 could provide a protective effect against LPS circulating in the blood of GVHD patients who receive BM-MSC infusions, ensuring a more predictable immunosuppressive effect. The novel effects and potential mechanisms following the stimulation of BM-MSC by DSP30 might impact their clinical use, by allowing the derivation of optimal "licensing" protocols for obtaining therapeutically efficient MSC. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

39. Expression of eight genes of nuclear factor-kappa B pathway in multiple myeloma using bone marrow aspirates obtained at diagnosis.

Author

Sampaio MS, Vettore AL, Yamamoto M, Chauffaille Mde L, Zago MA, and Colleoni GW

Subjects
Adult, Aged, Aged, 80 and over, Bone Marrow Cells metabolism, Case-Control Studies, Female, Humans, I-kappa B Proteins, Male, Middle Aged, Multiple Myeloma diagnosis, NF-KappaB Inhibitor alpha, NF-kappa B genetics, Pilot Projects, DNA-Binding Proteins metabolism, Gene Expression, Intercellular Adhesion Molecule-1 metabolism, Interleukin-6 metabolism, Multiple Myeloma metabolism, NF-kappa B metabolism, NF-kappa B p50 Subunit metabolism, Osteoprotegerin metabolism, RANK Ligand metabolism, Receptor Activator of Nuclear Factor-kappa B metabolism, Vascular Cell Adhesion Molecule-1 metabolism
Abstract

Purpose: To evaluate the expression of NF-kappaB pathway genes in total bone marrow samples obtained from MM at diagnosis using real-time quantitative PCR and to evaluate its possible correlation with disease clinical features and survival., Material and Methods: Expression of eight genes related to NF-kappaB pathway (NFKB1, IKB, RANK, RANKL, OPG, IL6, VCAM1 and ICAM1) were studied in 53 bone marrow samples from newly diagnosed MM patients and in seven normal controls, using the Taqman system. Genes were considered overexpressed when tumor expression level was at least four times higher than that observed in normal samples., Results: The percentages of overexpression of the eight genes were: NFKB1 0%, IKB 22.6%, RANK 15.1%, RANKL 31.3%, OPG 7.5%, IL6 39.6%, VCAM1 10% and ICAM1 26%. We found association between IL6 expression level and International Staging System (ISS) (p=0.01), meaning that MM patients with high ISS scores have more chance of overexpression of IL6. The mean value of ICAM1 relative expression was also associated with the ISS score (p=0.02). Regarding OS, cases with IL6 overexpression present worse evolution than cases with IL6 normal expression (p=0.04)., Conclusion: We demonstrated that total bone marrow aspirates can be used as a source of material for gene expression studies in MM. In this context, we confirmed that IL6 overexpression was significantly associated with worse survival and we described that it is associated with high ISS scores. Also, ICAM1 was overexpressed in 26% of cases and its level was associated with ISS scores.

Published
2009
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results