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42 results on '"Zaccai, Michele"'

15. Increased substrate availability reveals the potential of scentless lisianthus flowers in producing fragrant benzenoid‐phenylpropanoids.

Author

Fang, Fang, Oliva, Moran, Ovadia, Rinat, Bar, Einat, Nissim‐Levi, Ada, Kumar, Varun, Wang, Ru, Neeman, Agmon, Zaccai, Michele, Lewinsohn, Efraim, and Oren‐Shamir, Michal

Subjects
BENZYL alcohol, CUT flower industry, FLOWERING of plants, METHYL benzoate, BENZOATES, PLANT cuttings, BENZALDEHYDE
Abstract

Lisianthus (Eustoma grandiflorum), a leading plant in the cut flower industry, is scentless. Here we show that lisianthus flowers have potential to produce several fragrant benzenoid‐phenylpropanoids when substrate availability is not limited. To enable hyperaccumulation of substrates for the production of volatile benzenoid‐phenylpropanoids, lisianthus commercial hybrid "Excalibur Pink" was transformed via floral dipping with a feedback‐insensitive Escherichia coli DAHP synthase (AroG*) and Clarkia breweri benzyl alcohol acetyltransferase (BEAT), under constitutive promoters. The T1 progeny of "Excalibur Pink" plants segregated into four visual phenotypes, with pink or white colored petals and multiple or single petal layers. Interestingly, transformation with AroG* and BEAT caused no significant effect in the pigment composition among phenotypes, but did increase the levels of down‐stream fragrant volatile benzenoids. All the transgenic lines exclusively accumulated methyl benzoate, a fragrant benzenoid, either in their petals or leaves. Furthermore, feeding with benzyl alcohol resulted in the accumulation of two novel benzenoids, benzyl acetate (the product of BEAT) and benzoate, as well as a dramatic increase in the concentrations of additional benzenoid‐phenylpropanoid volatiles. Presumably, the degree of benzaldehyde overproduction after benzyl alcohol feeding in both leaves and flowers revealed their reverse conversion in lisianthus plants. These findings demonstrate the concealed capability of lisianthus plants to produce a wide array of fragrant benzenoid‐phenylpropanoids, given high substrate concentrations, which could in turn open opportunities for future scent engineering. [ABSTRACT FROM AUTHOR]

Published
2021
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16. Auxin regulates bulbil initiation by mediating sucrose metabolism in Lilium lancifolium

Author

Xin, Yin, Chen, Xi, Liang, Jiahui, Wang, Shaokun, Pan, Wenqiang, Wu, Jingxiang, Zhang, Mingfang, Zaccai, Michele, Yu, Xiaonan, Zhang, Xiuhai, Wu, Jian, and Du, Yunpeng

Abstract

Lily bulbils, which serve as advantageous axillary organs for vegetative propagation, have not been extensively studied in terms of the mechanism of bulbil initiation. The functions of auxin and sucrose metabolism have been implicated in axillary organ development, but their relationship in regulating bulbil initiation remains unclear. In this study, exogenous indole-3-acetic acid (IAA) treatment increased the endogenous auxin levels at leaf axils and significantly decreased bulbil number, whereas treatment with the auxin polar transport inhibitor N-1-naphthylphthalamic acid (NPA), which resulted in a low auxin concentration at leaf axils, stimulated bulbil initiation and increased bulbil number. A low level of auxin caused by NPA spraying or silencing of auxin biosynthesis genes YUCCA FLAVIN MONOOXYGENASE-LIKE 6(LlYUC6) and TRYPTOPHAN AMINOTRANSFERASERELATED 1 (LlTAR1) facilitated sucrose metabolism by activating the expression of SUCROSE SYNTHASES 1(LlSusy1) and CELL WALL INVERTASE 2(LlCWIN2), resulting in enhanced bulbil initiation. Silencing LlSusy1or LlCWIN2hindered bulbil initiation. Moreover, the transcription factor BASIC HELIX-LOOP-HELIX 35 (LlbHLH35) directly bound the promoter of LlSusy1, but not the promoter of LlCWIN2, and activated its transcription in response to the auxin content, bridging the gap between auxin and sucrose metabolism. In conclusion, our results reveal that an LlbHLH35-LlSusy1module mediates auxin-regulated sucrose metabolism during bulbil initiation.

Published
2024
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23. Tulipa gesneriana and Lilium longiflorum PEBP Genes and Their Putative Roles in Flowering Time Control

Author

Leeggangers, Hendrika ACF, primary, Rosilio-Brami, Tamar, additional, Bigas-Nadal, Judit, additional, Rubin, Noam, additional, van Dijk, Aalt DJ, additional, Nunez de Caceres Gonzalez, Francisco F, additional, Saadon-Shitrit, Shani, additional, Nijveen, Harm, additional, Hilhorst, Henk WM, additional, Immink, Richard GH, additional, and Zaccai, Michele, additional

Published
2017
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25. Regeneration and Transformation of Echinops cv Veitsch Blue

Author

Zaccai Michele, Reuveni Moshe, Chen Xinlu, and Evenor Dalia

Subjects
Echinops, Agrobacterium, fungi, food and beverages, GUS reporter system, Vernalization, Horticulture, Biology, biology.organism_classification, Cutting, Axillary bud, Botany, Shoot, Weed
Abstract

Echinops Asteraceae/Compositae is widespread throughout southern Europe and Asia. It is a native plant in Israel and usually found as a weed in wheat fields. Due to the need of the plants for vernalization, horticultural practice is to chill the crowns for 2 month before planting to achieve early flowering. A method for the propagation of Echinops through axillary buds and root cutting cultures is described in this manuscript. Best conditions for propagation were using MS salt medium supplemented with 3 sucrose and 1 mg l-1 IAA plus 0.5 mg l-11 BA under 16 h of cool fluorescent light. Root cuttings produced shoots all along the root but each growth regulator combination affected where roots will sprout shoots. Rooted plants were successfully acclimatized after propagating on the above medium and eventually flowered. Agrobacterium transformation of Echinops was successfully achieved and expression of GUS reporter gene was tested under the control of two constitutive promoters.

Published
2006

26. The proof is in the bulb: glycerol influences key stages of lily development.

Author

Lazare, Silit, Bechar, Daniel, Fernie, Alisdair R., Brotman, Yariv, and Zaccai, Michele

Subjects
EASTER lily, VERNALIZATION, MERISTEMS, PLANT development, METABOLITES
Abstract

Summary: A bulb is a whole plant condensed into an underground organ. A geophyte's bulb comprises both food reserves and important developmental history that may affect its whole growth. In Easter lily (Lilium longiflorum), bulb size is associated with the plant's flowering pathway – vernalization or photoperiod – and also affects sprouting, flower quality and abortion rate. The aim of this study was to investigate the reasons for the major physiological differences between large and small bulbs. Lily bulbs start their development from secondary meristems along the stem, with large bulbs being heavier and bear more scales than small ones. Peeling the outer scales of a large bulb converts its physiological responses into those of a small bulb, implying that the physiological discrepancies in plants developing from large or small bulbs are mediated by factors inherent to the bulb. We therefore performed broad analyses of the metabolite composition in the scales of bulbs subjected to temperature regimes affecting further plant development. We found a striking association between the level of glycerol, a primary metabolite mostly synthesized in the outer scales, and a delay in sprouting and flowering time, and reduction in abortion rate. Exogenous glycerol application to the bulbs before planting corroborated these results. Moreover, transcriptome analyses showed that flowering‐promoting gene expression was downregulated in the bulb after glycerol treatment, while potential flowering inhibitor as well as a dormancy‐related gene expressions were upregulated. Based on these studies, we postulate that glycerol is a major factor influencing both vegetative and reproductive development in lily. Significance statement: In lily, bulb size is linked with the plant's flowering mechanism, vernalization or photoperiod, and affects important parameters during plant development. We show that glycerol, which is mainly produced in large bulbs, is strongly associated with physiological differences between large and small bulbs and is a major factor influencing both vegetative and reproductive development in lily. [ABSTRACT FROM AUTHOR]

Published
2019
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