Search

Plant wax ester synthase catalyzes the synthesis of wax esters from long-chain alcohols and fatty acids, and plays very important roles in plant wax synthesis and some resistances to drought, pathogenic bacteria, ultraviolet radiation, cold and insect invasion and other environmental stresses; cadmium (Cd) is one of the toxic heavy metals with the highest content in environment, and seriously threatens plant growth, development, quality, yield, and plant food safety. In order to explore the Cd stress expressions of wax ester synthase genes in Rehmannia glutinosa, we identified its wax ester synthase genes from its full-length transcriptome sequencing data, analyzed both physiochemical properties, phytogenetic trees and conserved domains with bioinformatics methods, and tissue expressions and Cd stress expressions using qRT-PCR. The results were as follows: (1) Two wax ester synthase genes, named as RgOATWSD1 and RgOATWSD2, were identified, whose coding proteins were unstable hydrophobic proteins with amino acid lengths of 463 aa and 473 aa, isoelectric points of 8.86 and 9.34 and molecular weights of 51.31 kD and 52.49 kD, respectively. (2) Both proteins contained a conserved acyl_WS_DGAT domain and DUF1298 superfamily, in which the former accounted for 92.65% to 94.50% of the amino acid sequence. (3) Both proteins were located in the endoplasmic reticulum and both secondary structures were mainly composed of random coil and α-helix;RgOATWSD2 was not transmembrane protein but RgOATWSD1. (4) Both were differentially expressed in the roots, stems and leaves of R. glutinosa plants. (5) Both expressions were highly responsive to Cd stress, but both expression change trends were different under Cd stress. This study identifies two wax ester synthase genes in response to Cd stress, and lays a foundation for further research on Cd stress expression and other functions of RgOATWSD.

In the process of offline first-class graduate course construction, teachers should update the teaching idea, explore the curriculum reform, strengthen the classroom teaching, innovative teaching mode, promote teaching, with student development as the centre, is committed to tap students 'inner potential and stimulate learning motivation, pay attention to students' morality, intellectual and physique all-round development, ensure the teaching quality. In order to cultivate students' professional ability and innovation ability, the article first of the modern agricultural innovation and rural revitalization of strategic course construction content introduction, and then expounds the modern agricultural innovation and rural revitalization of strategic course construction ideas, finally discusses the modern agricultural innovation and rural revitalization strategy course as offline first-class graduate course construction practice.

Promoting electric vehicles (EVs) adoption has become one of the important paths for countries around the world to address climate change and accelerate the transformation of energy system for achieving sustainable development. As one of the important psychological factors, the research on the explanatory power of emotions to EVs purchase intention is still insufficient. This paper collected 400 valid questionnaires all around China. By incorporating emotions and moral norms into the Theory of Planned Behavior (TPB) model, this study used structural equation model to estimate the impact of positive anticipated emotion (PAE), negative anticipated emotion (NAE), and moral norms together with TPB elements on EVs purchase intention. In order to explore the heterogeneity effect of the above factors on EVs purchase intention among consumers of different income groups, we divided the total sample into high-income subsample and low-income subsample according to the household monthly disposable income. We concluded as follows: for the total sample, PAE has the greatest impact on EVs purchase intention, followed by attitude, NAE, and perceived behavioral control (PBC). In particular, the purchase intention of high-income consumers mainly depends on NAE, while the purchase intention of low-income consumers mainly depends on PAE. Additionally, PBC has more significant impact on EVs purchase intention of high-income group. Finally, targeted policy implications are proposed to promote EVs purchase., Author(s): Zhengxia He [sup.1] , Yanqing Zhou [sup.4] , Jianming Wang [sup.2] , Wenxing Shen [sup.3] , Wenbo Li [sup.4] , Wenqi Lu [sup.4] Author Affiliations: (1) grid.410595.c, 0000 0001 [...]