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Objective To investigate the risk factors influencing the incidence of chemotherapy-induced myelosuppression (CIM) following first-line chemotherapy in patients with moderately advanced colorectal cancer (CRC), and to develop and validate a nomogram to assess the risk of CIM in the patients.Methods The clinical data of patients with stage II-IV CRC who received first-line chemotherapy at Anning First People's Hospital affiliated with Kunming University of Science and Technology between July 2021 and January 2024, were retrospectively analyzed. The World Health Organization classification standard for acute and subacute toxicity of anticancer drugs were used as the standards for diagnosing CIM, and the patients were subsequently categorized into CIM and non-CIM groups. The risk factors for CIM were analyzed and a multivariate Logistic regression model was employed to construct the predictive model. The model's discrimination and accuracy were assessed using the receiver operating characteristic curve (ROC) and area under curve (AUC) and the Hosmer-Lemeshow goodness of fit test. Additionally, the model's clinical utility was evaluated using calibration and clinical decision curves.Results A total of 257 patients were included, 112 individuals exhibited bone CIM, corresponding to an incidence rate of 43.58%. The most prevalent severity was classified as grade I-II CIM. Multivariate Logistic regression analysis identified the chemotherapy cycle, reductions in white blood cell count, hemoglobin levels, and platelet count prior to chemotherapy as independent risk factors for CIM in CRC patients (P

AIM: To analyze the clinical outcomes of cyclosporine combined with lacrimal plug in the treatment of dry eye in patients with primary Sjögren's syndrome.METHODS: The clinical data of 60 patients(120 eyes)who were admitted to the ophthalmology department and rheumatology and immunology department of Baoding No.1 Central Hospital and were diagonosed with siogren's syndrome dry eye after multidisciplinary consultation from June 2022 to September 2023 were retrospectively analyzed. All the patients received regular treatment of primary Sjögren's syndrome, and they were divided into three groups according to treatment methods: A, B and C, with 20 cases(40 eyes)in each group. The group A received 0.3% sodium hyaluronate eyedrops, the group B received 0.3% sodium hyaluronate eyedrops plus 0.05% cyclosporine eyedrops, and the group C received 0.3% sodium hyaluronate eyedrops plus 0.05% cyclosporine eyedrops combined with binocular lacrimal plugs. The ocular surface disease index(OSDI)score, conjunctival hyperemia score, tear film breakup time(BUT), tear meniscus height(TMH), corneal fluorescein staining(FL)score and tear secretion of the three groups of patients were compared before and at 4, 8 and 12 wk after treatment. The contents of inflammatory factors such as interleukin-6(IL-6), tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in tears were detected before and at 12 wk after treatment.RESULTS: At 4, 8 and 12 wk after treatment, the scores of OSDI, conjunctival hyperemia score and FL in the three groups of patients were lower than those before treatment, and the BUT, TMH and tear secretion were higher than those before treatment(all P0.05).CONCLUSION: The combined use of cyclosporine and lacrimal plug is safe and effective in improving the clinical symptoms of patients with moderate and severe dry eye, promoting the function of tear film and cornea, increasing tears secretion, and reducing the level of tear inflammatory factors.

The molecular dynamics method was used to simulate the microstructure dynamic evolution， dislocation， and pore motion characteristics of AlCoCrFeNi high-entropy alloy at temperature 300 K and strain rate of 1×109 s-1， and the failure mechanism was revealed. The simulation results show that the maximum load-bearing， longitudinal modulus， and ductility of the nano-polycrystalline AlCoCrFeNi high-entropy alloy are lower than those of nano-monocrystalline. The strain reduction and peak stress reduction of nano-polycrystalline before yield are 25% and the peak stress reduction is 23.8%. The phase transition， dislocation， hole， and failure mechanism of the two nanocrystallines are different during the stretching process. During the stretching process of nano-monocrystals， the FCC structure is mainly transformed into a non-crystalline structure. The atomic position changes after the phase change， accompanied by a large number of Shorkly dislocations， and moves with the growth direction of the non-crystalline structure. The hole nucleation， growth， penetration， and failure fracture of non-crystalline structure area are mainly amorphous perforation fault. During the stretching process of nano-polycrystalline， the FCC structure mainly transforms to HCP structure and non-crystalline structure， and the atomic position changes after the phase change， accompanied by a large number of 1/6〈112〉 （Shortly） dislocations and a small number of 1/6〈110〉 （Stair-rod） dislocations， 1/3〈100〉 （Hirth） dislocations， and other dislocations continue to be generated and annihilated.The material undergoes certain plastic deformation， with nucleation of pores in the non-crystalline structure area of the grain boundary， growth and expansion along the grain boundary， and penetration through the grain boundary until failure fracture， showing mainly intergranular fracture.

To explore the effect of peer education on improving the knowledge, attitude, practice level (KAP) to prevent human papilloma virus (HPV) infection among college students.

Abstract Integrating the Knowledge Graphs (KGs) into recommendation systems enhances personalization and accuracy. However, the long-tail distribution of knowledge graphs often leads to data sparsity, which limits the effectiveness in practical applications. To address this challenge, this study proposes a knowledge-aware recommendation algorithm framework that incorporates multi-level contrastive learning. This framework enhances the Collaborative Knowledge Graph (CKG) through a random edge dropout method, which constructs feature representations at three levels: user-user interactions, item-item interactions and user-item interactions. A dynamic attention mechanism is employed in the Graph Attention Networks (GAT) for modeling the KG. Combined with the nonlinear transformation and Momentum Contrast (Moco) strategy for contrastive learning, it can effectively extract high-quality feature information. Additionally, multi-level contrastive learning, as an auxiliary self-supervised task, is jointly trained with the primary supervised task, which further enhances recommendation performance. Experimental results on the MovieLens and Amazon-books datasets demonstrate that this framework effectively improves the performance of knowledge graph-based recommendations, addresses the issue of data sparsity, and outperforms other baseline models across multiple evaluation metrics.

Objective To investigate the imaging diagnosis and differential diagnosis methods for common tumors in the posterior fossa of children, and to propose a flow chart for the diagnosis of common tumors in the posterior fossa of children based on the limited diffusion of DWI according to the idea of localization diagnosis followed by qualitative diagnosis. Methods A total of 118 pediatric patients with posterior fossa tumors who underwent surgical resection in Huashan Hospital, Fudan University from January 2021 to January 2024 were enrolled, and all of them underwent head CT and MRI examinations, and preoperative localization diagnosis and qualitative diagnosis were carried out accordingly. Then, based on whether DWI was diffusion limited, a flow chart for the diagnosis of common tumors in the posterior fossa of children was proposed. Results A total of 118 children with posterior fossa tumors were localization and qualitatively diagnosed, including 41 cases of fourth ventricular tumors, including medulloblastoma (27 cases), pilocytic astrocytoma (7 cases), ependymoma (5 cases), choroid plexus papilloma (one case), and rosette-forming glioneuronal tumor (RGNT, one case). There were 38 cases of brainstem tumors, including 24 cases of diffuse midline glioma, H3 K27 - altered, 5 cases of pilocytic astrocytoma, 3 cases of cavernous hemangioma, 2 cases of pediatric-type diffuse high-grade glioma (prone to diffuse midline glioma, H3 wild type), 2 cases of ganglio glioma, one case of atypical teratoid/rhabdoid tumor (AT/RT), and one case of pediatric - type diffuse low - grade glioma. There were 9 cases of cerebellopontine angle (CPA) tumors, including 3 cases of pilocytic astrocytoma, 2 cases of cholesteatoma, one case of medulloblastoma, one case of diffuse midline glioma, H3 K27-altered, one case of pilomyxoid astrocytoma, and one case of Ewing sarcoma. There were 30 cases of cerebellar tumors, including 15 cases of pilocytic astrocytoma, 7 cases of medulloblastoma, 2 cases of cavernous hemangioma, one case of ependymoma, one case of pediatric-type diffuse low-grade glioma, one case of pediatric-type diffuse high- grade glioma (prone to diffuse midline glioma, H3 wild type), one case of dysembryoplastic neuroepithelial tumor (DNT), one case of hamartoma, and one case of extrarenal rhabdomyomatoid tumor cerebellar metastases. Localization diagnosis, the accuracy of preoperative CT was 93.22% (110/118), while the accuracy of preoperative MRI was 100% (118/118). Qualitative diagnosis, 74 cases (62.71%) had accurate qualitative diagnosis, 23 cases (19.49%) had general diagnosis, and 21 cases (17.80%) had wrong diagnosis. The imaging differential diagnosis of posterior fossa tumors in children focuses on medulloblastoma, pilocytic astrocytoma, ependymoma, and diffuse midline glioma, H3 K27-altered. The incidence of limited diffusion of DWI in medulloblastoma, pilocytic astrocytoma and ependymoma outside the brainstem was 100% (35/35), 4% (1/25) and 5/6, and the difference among the three tumors was statistically significant (Z = - 5.601, P = 0.000). The incidence of limited diffusion of DWI in diffuse midline glioma, H3 K27- altered and pilocytic astrocytoma in the brainstem was 79.17% (19/24) and 1/5, and the difference between the two tumors was also statistically significant (Fisher's exact possibility: P = 0.038). Conclusions The early diagnosis of posterior fossa tumors in children is difficult, and DWI is an important basis for differential diagnosis.

BackgroundAs society ages, the incidence of osteoporotic vertebral compression fractures steadily rises. Procedures like percutaneous kyphoplasty (PKP) and percutaneous vertebroplasty (PVP) have proven effective in significantly relieving pain in patients with these fractures. While PKP and PVP are minimally invasive, complications can still occur. However, most complications are not clinically significant, with cement leakage being the most common.Case presentationWe present the case of a patient with an osteoporotic vertebral compression fracture who underwent percutaneous kyphoplasty (PKP) and percutaneous vertebroplasty (PVP). On the night following the procedure, the patient experienced transient discomfort in the chest, which resolved on its own. A chest CT scan the next day revealed a 5 cm arc-shaped high-density shadow near the right atrium, along with multiple high-density lung spots. After consulting with cardiothoracic surgery, interventional vascular surgery, and radiology experts, and discussing options with the patient and their family, a thoracotomy was recommended to remove the bone cement from the heart. However, the attempt was unsuccessful. Despite this, the patient made a good recovery and was successfully discharged.ConclusionsVascular leakage of bone cement is a potentially life-threatening complication of PKP/PVP, and it warrants careful attention.

Abstract As the predominant pest of alfalfa, Odontothrips loti Haliday causes great damages over the major alfalfa-growing regions of China. The characteristics of strong mobility and fecundity make them develop rapidly in the field and hard to be controlled. There is a shortage of bioinformation and limited genomic resources available of O. loti for us to develop novel pest management strategies. In this study, we constructed a chromosome-level reference genome assembly of O. loti with a genome size of 346.59 Mb and scaffold N50 length of 18.52 Mb, anchored onto 16 chromosomes and contained 20128 genes, of which 93.59% were functionally annotated. The results of 99.20% complete insecta_odb10 genes in BUSCO analysis, 91.11% short reads mapped to the ref-genome, and the consistent tendency among the thrips in the distribution of gene length reflects the quality of genome. Our study provided the first report of genome for the genus Odontothrips, which offers a genomic resource for further investigations on evolution and molecular biology of O. loti, contributing to pest management.

Lung cancer (LC) is regarded as a fatal cancer, and insulin-like growth factor 1 (IGF1) and its receptor (IGF1R) have been found to play a key role in regulating tumor glycolytic metabolism. The aim of this study is to investigate LC proliferation regulated by metabolite-mediated IGF1R lactylation. IGF1R was highly expressed in LC tissues and cells, and the effects of IGF1R on protein stability were inhibited by Lactate dehydrogenase A (LDHA) inhibition. Moreover, the tightness of IGF1R binding to IGF1 was also enhanced by exogenous lactic acid but suppressed by LDHA silencing, while cell viability and proliferation were promoted by over-expression of IGF1R. Exogenous lactic acid further exacerbated the effects of the IGF1R gene, while LDHA knocking down reduced the IGF1R-induced malignant behaviors. The IGF1R and exogenous lactic acid were also found to increase extracellular acidification rate (ECAR) and decrease oxygen consumption rate to regulate glycolysis, which was inhibited by LDHA deficiency in LC cells. The study concluded that IGF1R-mediated aggressive behaviors of LC cells were associated with higher levels of IGF1R lactylation. Moreover, lactic acid can improve the protein stability of the IGF1R oncogene, thus promoting glycolysis and generating lactic acid, forming a closed loop. Therefore, targeting IGF1R is envisaged to provide a novel strategy for developing therapeutic agents against LC.

AIM:To measure the superficial capillary plexus(SCP)vessel density(VD), deep capillary plexus(DCP)VD, and the area, circumference, and roundness index of the foveal avascular zone(FAZ)in healthy individuals of four ethnic groups, namely, Uyghur, Han, Kazakh, and Hui by optical coherence tomography angiography(OCTA), and to investigate the differences of blood flow parameters in macular area of healthy individuals among different ethnic groups in China.METHODS: A total of 80 cases(80 eyes)of healthy subjects if each of the four ethnic groups who went to the Eye Center of the Friendship Hospital of Ili Kazak autonomous Prefecture from December 2022 to March 2023 and met the criteria were selected for the study, with 320 eyes totally. The patients were grouped and numbered according to their ethnicity, and the spherical equivalent and axial length were obtained by using an autorefractor and IOL Master. The blood flow images of the macular area in the range of 3 mm×3 mm were obtained by using a DRI Triton OCT detector, and the images were analyzed by using the built-in IMAGENET6 software to obtain the blood flow parameters. Furthermore, the differences in the blood flow parameters between different ethnic groups as well as the effects of gender, age, and axial length on macular blood flow parameters of different ethnic groups were compared.RESULTS:There were no statistically significant differences in gender, age, axial length, diopter, and image quality(IQ)among the four groups of subjects(all P>0.05). Ethnic differences: in SCP, Uyghur inferior VD is higher than Kazakh, Hui inferior VD is higher than Han and Kazakh, Han and Hui nasal VD is higher than Uyghur, Kazakh foveal VD is higher than Han and Hui, Kazakh FAZ area is smaller than the other 3 ethnic groups, Han and Hui FAZ perimeter is larger than Kazakh, and Hui FAZ circularity index is lower than the other 3 ethnic groups; in DCP, Uyghur foveal VD is higher than Han and Hui, Kazakh foveal VD is higher than Han and Hui, Kazakh FAZ area and perimeter are smaller than the other 3 ethnic groups and Kazakh FAZ circularity index is higher than the other 3 ethnic groups(all P

Abstract A low complexity resource allocation method is proposed for downlink non‐orthogonal multiple access system assisted by intelligent reflecting surface (IRS). Firstly, an optimisation problem is formulated to minimise power consumption, with power allocation and IRS phase shifts as variables. Then, the joint optimisation problem of power allocation and IRS phase shifts is transformed into the optimisation of IRS phase shifts, which is further decomposed into multiple single variable sub‐problems, the solutions to which can be obtained by using the function extremum method. Next, based on these sub‐problems, one‐level iterative algorithm is developed to optimise IRS phase shifts. Finally, the minimum power required by each user is calculated based on the IRS phase shifts obtained through iteration. Simulation results show that the proposed scheme is better than the existing schemes in the same scenario under the same rate requirement in terms of computational complexity and power consumption.

Spontaneous subarachnoid hemorrhage (SAH) is a kind of hemorrhagic stroke which causes neurological deficits in survivors. Huperzine A has a neuroprotective effect, but its role in SAH is unclear. Therefore, we explore the effect of Huperzine A on neurological deficits induced by SAH and the related mechanism. In this study, Evans blue assay, TUNEL staining, immunofluorescence, western blot analysis, and ELISA are conducted. We find that Huperzine A can improve neurological deficits and inhibit the apoptosis of nerve cells in SAH rats. Huperzine A treatment can improve the upregulation of brain water content, damage of blood-brain barrier, fibrinogen and matrix metalloprotein 9 expressions and the downregulation of ZO-1 and occludin expressions induced by SAH. Huperzine A inhibit the expressions of proteins involved in pyroptosis in endothelial cells in SAH rats. The increase in MDA content and decrease in SOD activity in SAH rats can be partly reversed by Huperzine A. The ROS inducer H2O2 can induce pyroptosis and inhibit the expressions of ZO-1 and occludin in endothelial cells, which can be blocked by Huperzine A. In addition, the increase in the entry of p65 into the nucleus in endothelial cells can be partly reversed by Huperzine A. Huperzine A may delay the damage of blood-brain barrier in SAH rats by inhibiting oxidative stress-mediated pyroptosis and tight junction protein expression downregulation through the NF-κB pathway. Overall, Huperzine A may have clinical value for treating SAH.

Abstract This paper aims to propose a table tennis target detection (TD) method based on deep learning (DL) and multi-scale feature fusion (MFF) to improve the detection accuracy of the ball in table tennis competition, optimize the training process of athletes, and improve the technical level. In this paper, DL technology is used to improve the accuracy of table tennis TD through MFF guidance. Initially, based on the FAST Region-based Convolutional Neural Network (FAST R-CNN), the TD is carried out in the table tennis match. Then, through the method of MFF guidance, different levels of feature information are fused, which improves the accuracy of TD. Through the experimental verification on the test set, it is found that the mean Average Precision (mAP) value of the target detection algorithm (TDA) proposed here reaches 87.3%, which is obviously superior to other TDAs and has higher robustness. The DL TDA combined with the proposed MFF can be applied to various detection fields and can help the application of TD in real life.

Abstract Purpose The adverse health consequences of premarital sex and childhood sexual abuse (CSA) are both global public health problems. Based on a random sample of college students from a Chinese city, this study investigated the relationship between CSA severity and premarital sex among students, focusing on sex differences. Methods A total of 2722 college students from 4 schools in Luzhou were recruited by multistage random sampling. Self-administered questionnaires were used to measure CSA experiences and premarital sex. Binary logistic regression analyses were conducted to analyse the relationship between CSA and premarital sex. Results The prevalence of CSA was 9.39%, and that of mild, moderate and severe CSA was 4.04%, 2.90% and 2.46%, respectively. The premarital sex reporting rate was 22.42%. After adjusting for confounding variables, CSA was positively associated with premarital sex. Notably, a cumulative effect of CSA on premarital sex was observed among students. Further stratification analyses showed that males who experienced CSA had a higher premarital sex rate than females, and this sex difference was also observed among students with different CSA severities. Conclusion CSA and its severity were associated with premarital sex among college students. Furthermore, this association was stronger for males than females. Therefore, it is important to emphasize CSA prevention, especially for boys. These findings can promote understanding of the effects of CSA on premarital sex, and CSA prevention and intervention strategies should consider CSA severity and sex differences.

ObjectiveTo ascertain the causes of a food poisoning incident and provide references for the prevention of similar incidents in the future.MethodsCase investigation was conducted through field epidemiological investigation methods， and suspicious meals and foods were searched by the analytical epidemiological method. A food hygiene investigation was conducted in the establishment involved and samples of suspicious food， processing steps， and cases were collected for laboratory testing.ResultsA total of 91 individuals meeting the case definition were identified， resulting in an attack rate of 14.97% （91/608）. The main clinical manifestations included abdominal pain （97.80%）， diarrhea （84.62%）， nausea （62.64%）， vomiting （72.53%）， fever （12.09%）， and increased white blood cells （90.11%）. The peak incidence occurred from 16：00 to 18：00 on June 15. The epidemic curve showed a point-source exposure pattern， with an incubation period of 1 h minimum and 10 h maximum， and an average of 5 h. Analytical epidemiological studies indicated that lunch on June 15 was the suspicious meal （χ2=38.78， P

Objective·To screen the mutations of NEUROD1 gene in families of maturity-onset diabetes of the young (MODY), and investigate the correlation between the mutation and MODY6 and its potential pathogenesis in Chinese.Methods·PCR-direct sequencing was used for screening NEUROD1 mutations from 96 MODY probands who were negative for mutations in the GCK/MODY2, HNF1A/MODY3 and HNF1B/MODY5 genes, and the genotypic frequency of NEUROD1 variations were compared between the 96 MODY probands and 100 non-diabetic control subjects. A de novo modeling method was used to predict the three-dimensional (3D) structures of wild type (WT) and mutated NEUROD1 proteins. Transcriptional activities of both WT and mutant of NEUROD1 on insulin gene were detected by using dual luciferase reporter gene system.Results·Glu59Gln (NM_002500.5, c.175G>C), a heterozygous missense mutation in the NEUROD1 gene, was identified in a MODY pedigree. 3D structural analysis showed that the mutation transformed the negatively charged Glu59 of WT into uncharged mutation Gln59, leading to the loss of Glu59-Arg54 and Glu59-Lys88, two salt bridge bonds, and the formation of Gln59-Arg54, one new hydrogen bond. Transcriptional activity of Glu59Gln mutant for insulin gene was reduced by 36.3% when compared with that of WT (P

Objective·To screen mutations of key genes in the leptin-melanocyte stimulating hormone (LEP-MSH) pathway by whole-exome sequencing (WES) in the obese cohort.Methods·A total of 119 obese patients aged 17－65 years old with body mass index (BMI)≥34 kg/m2, who underwent laparoscopic sleeve gastrectomy from January 2011 to July 2019 at Shanghai Sixth People′s Hospital, Shanghai Jiao Tong University School of Medicine were collected. The peripheral blood samples of the research subjects were collected, and whole genome DNA was extracted to perform WES. Bioinformatic methods were applied to detect the mutations in 16 genes in the LEP-MSH pathway (ADCY3, AGRP, BDNF, KSR2, LEP, LEPR, MC3R, MC4R, MCHR1, MRAP2, NTRK2, PCSK1, PHIP, POMC, SH2B1, and SIM1). Rare variants with the minor allele frequency in the total population less than 0.02 and in the East Asian population less than 0.01 in the 1000 Genome (1000G), Exome Aggregation Consortium (ExAC) and Genome Aggregation Database (gnomAD) were selected for subsequent analysis. Six pieces of prediction software were used to evaluate the deleteriousness of the mutations. Finally, based on the clinical information of each patient, the pathogenicity of all variants was determined according to the guidelines of America College of Medical Genetics and Genomics (ACMG), and only the "pathogenic", "likely pathogenic", and "uncertain significance" variants were retained.Results·A total of 26 variants, 22 kinds of variants were detected in 24 patients from 119 subjects, all of which were heterozygous mutations. The detected variants included 7 in SH2B1 gene (accounting for 26.92% of the total variants), 4 in MCHR1 gene (accounting for 15.38%), 3 in PHIP gene (accounting for 11.53%), 2 in ADCY3 and LEPR genes (accounting for 7.69%, respectively), and 1 in LEP, NTRK2, AGRP, KSR2, MC3R, MC4R, BDNF, and PCSK1 genes, respectively (accounting for 3.85%, respectively). There were 3 patients having the same mutation site in SH2B1 gene, and 2 patients having the same mutation sites in LEPR gene and MCHR1 gene, respectively. In addition, among these mutations, there were 12 ones not included in the East Asian population in 3 public databases, which were novel mutations in the East Asian population, located in SH2B1 (p.V209I, p.R67C, and p.L149F), KSR2 (p.P155T), LEP (p.D106N), LEPR (p.W132R), PHIP (p.K1461R), BDNF (p.N84S), PCSK1 (p.R282W), NTRK2 (p.T732M), MC3R (p.S71P), and MC4R (p.W174X).Conclusion·A total of 22 kinds of rare variations possibly associated with obesity in the LEP-MSH pathway are detected, 12 of which are novel in the East Asian population.

Objective·To identify the causative gene and mutations and describe the clinical traits in a Chinese diabetes pedigree suspected of Wolfram syndrome.Methods·A total of 12 subjects from one family were included. The proband was admitted to the Department of Endocrinology, The First Affiliated Hospital of Xinxiang Medical University, for the first time in May 2013. Then he visited the hospital for follow-up in July 2022 and in April 2023, respectively. The other members of this family included the proband′s sister, father, mother, paternal grandfather, paternal grandmother, uncle, aunt, as well as maternal grandfather, maternal grandmother, and two brothers of the proband′s mother. Clinical data of all subjects were collected. The whole exome sequencing was used to screen the pathogenic genes and mutation sites of six members of the family, and Sanger sequencing was used to verify the above results. Effects of the mutation of the pathogenic gene WFS1 in Wolfram syndrome on the function of the wolframin protein were evaluated by bioinformatics softwares, including CADD, DANN, MetaSVM, Polyphen-2, SIFT and M-CAP. The three-dimensional structures of wild-type and mutant wolframin proteins were constructed with Swiss-Model software, and visualized with PyMOL software. Cluster Omega software was used for evaluating species conservation of WFS1 gene mutation sites. JNetPRED software was used for online prediction of wolframin protein secondary structure.Results·The proband and his sister both carried R558H and S411Cfs*131 mutations, two compound heterozygous mutations of the Wolfram syndrome pathogenic gene WFS1. The proband′s father and parental grandfather both carried the R558H mutation, while the proband′s mother and maternal grandfather both carried the S411Cfs*131 mutation. The R558H mutation was a rare missense mutation, and the S411Cfs*131 mutation was a novel frameshift mutation. Bioinformatics analysis softwares predicted that the R558H mutation located in the α-helical structure of the wolframin protein. This mutation was a damage mutation and the amino acid sequence of the mutation region was highly conservative among 12 species with varying degrees of evolution, including humans.Conclusion·Two causative mutations of WFS1 gene are identified in a Chinese diabetes pedigree by whole exome sequencing. The study supplements the existing genotype and phenotype profiles of Wolfram syndrome, which can realize early diagnosis of diabetes pedigrees and help in performing timely follow-up of patients, so as to achieve early intervention and treatment of this disease.

In order to improve the reliability of two-dimensional nuclear magnetic resonance (2D NMR) measurement in shale oil reservoirs, the T2-T1 2D NMR response characteristics and influencing factors of shale oil reservoirs were analyzed in three scenarios: laboratory core analysis, wellsite mobile full-diameter core scanning and logging. For different application scenarios, the optimization method of T2-T1 NMR measurement parameters for shale oil reservoirs were proposed. Laboratory core measurements require attention to the choice of echo interval time (TE) and number of echo groups, in addition to the different magnetic field strengths and measurement sequences. When scanning full-diameter cores at the well site, it is necessary to focus on TE and minimum waiting time (Tw). To ensure the convergence in T1 dimension of quickly relaxed component, the minimum Tw should be set to at least 1 ms. NMR logging is limited by the acquisition conditions. It is necessary to focus on the distribution range and smoothing factor in data processing to interpret and modify data with different signal-to-noise ratios. A poor shale oil reservoir with NMR porosity less than 5% is difficult to ensure the accuracy of NMR results for its low signal-to-noise ratio. The systematic analysis of T2-T1 2D NMR measurement parameters provides a reference for the optimization of 2D NMR detection methods for shale oil reservoirs, which helps to improve the accuracy of NMR measurements and obtain more reliable reservoir information.

Objective To explore the clinical value of dual-source virtual non-contrast (VNC) CT combined with artificial intelligence (AI) in the diagnosis of acute intracranial hemorrhage. Methods A cross-sectional study was conducted on the imaging data of non-contrast CT and dual-energy CT angiography (CTA) in 67 patients with suspected intracranial hemorrhage. They were 32 males and 35 females, at an average age of 56.2±12.5 (35~89) years. The differences of average CT value, noise, signal to noise ratio (SNR), contrast noise ratio (CNR) and radiation dose between VNCT and NCCT were analyzed. Taken the common diagnosis results of two radiologists as the reference standard, the type of intracranial hemorrhage, the degree of midline structural deviation, the long and short diameters of the largest hemorrhage layer and the volume of hematoma were analyzed by AI software under VNCT and NCCT images. And the time taken by radiologists and AI to obtain the above quantitative evaluation indexes was compared. Results In the objective evaluation, the average CT value, SNR and CNR were significantly lower (P < 0.001), and the noise was higher in the VNCT images than the NCCT images (P < 0.001). The radiation dose of VNCT was lower by 75.6% than that of NCCT+CTA (P < 0.001). The qualitative analysis indicated that the accuracy of AI in diagnosing intracranial hemorrhage on NCCT and VNCT images was 94.0% and 89.6% respectively. The accuracy rates of VNCT+AI for IPH, IVH, SAH and SDH were 83.6%, 86.0%, 83.0% and 60.0%, respectively, which had no differences when compared with those of NCCT+AI (all P>0.05). In the quantitative analysis, VNCT+AI had good consistency with manual measurement results in terms of the above indicators (ICC=0.820, 0.942, 0.920, and 0.959, P < 0.001). In terms of the time required for AI diagnosis, VNCT need significantly shorter time than NCCT(50.6±15.9 vs 58.5±24.9 s, P < 0.001). Conclusion Dual-source VNC technology has low radiation dose, simple and fast examination process, and high accuracy in the diagnosis of acute intracranial hemorrhage when combined with AI. And the quantitative evaluation indexes of VNCT+AI are in good consistency with radiologists and has potential clinical application value.

The planetary gear train is an important rotating mechanical component, and its early faults diagnosis is an important means to avoid major accidents. Early faults diagnosis of planetary gear trains is bound to become the focus of scholars. The characteristics of early faults signals of planetary gear trains are analyzed, the research status of the early faults mechanism, signal acquisition methods, faults feature extraction and identification of planetary gear trains are combed, and the main problems in early faults diagnosis are summarized. Some suggestions are put forward, such as the research of early compound faults mechanism based on the dynamic model, the research of early faults signal acquisition method based on the multi-sensor, the research of early faults characteristic index based on multi-domain analysis, the research of early faults diagnosis based on numerical model and so on. It can be used as a reference for further research on early faults diagnosis of planetary gear trains.

ObjectiveTo understand the health-related life quality and its influencing factors in elderly community residents in Shanghai， and to provide reference for improving their life quality.MethodsDuring April to June 2021， a cross-sectional survey was conducted in five selected community health care centers， from Baoshan District， Minhang District， Songjiang District and Pudong New District of Shanghai， respectively. The elderly residents aged 65 and over who received physical examination were face-to-face interviewed using EQ-5D-5L scale. SPSS 26.0 and Stata 16 were used for statistical analysis. Binary logistic regression， multiple linear regression and Tobit regression were used to analyze influencing factors of each dimension in EQ-5D scale， EQ-VAS score and health utility value.ResultsA total of 1302 elderly residents were included in the study， and the proportions of problems in the five dimensions of EQ-5D-5L scale were pain/discomfort （13.9%）， mobility （9.1%）， daily activity （5.8%）， self-care ability （4.8%） and anxiety/depression （3.6%）， while age was a factor influencing problems in each dimension. The average score of EQ-VAS was 84.28±10.32， and the average health utility value based on Chinese population was 0.97±0.08， which was higher than the rest of the country. Tobit regression analysis showed that female （t=-3.96， P

The significance of the existence of clinical practice guidelines lies in their implementation, however, there are still many problems in the implementation of guidelines and bottlenecks in the translation of clinical evidence. The introduction of knowledge graph technology provides a solid foundation for guideline intel-ligence and a good tool for solving guideline implementation problems. The combined Traditional Chinese Medi-cine (TCM)/ Integrated Traditional Chinese and Western Medicine (IM) reflect the characteristics of the healthcare system in China. Using the current status of TCM/IM diagnosis and treatment of cardiovascular diseases and the current problems in the application of TCM/IM guidelines for cardiovascular diseases, this study defined the objectives of constructing a knowledge graph for TCM/IM clinical guidelines for cardiovascular diseases, and accordingly designed a knowledge graph framework for TCM/IM clinical guidelines that is con-sistent with professional knowledge and practical application. We analyzed the scope of the guidelines, ar-ranged their content and summarized their knowledge structure. We then established concept structure tables and entity semantic relationship tables. The design of the knowledge graph framework for TCM/IM guidelines proposed in this paper provides a basis for the digitization and communication of guidelines, and is conducive to promoting their implementation and dissemination.

Abstract Background In recent years, the number of adolescents engaging in premarital sex has increased, and an increasing number of childhood sexual abuse (CSA) cases have been reported in China. Many studies have indicated that CSA has a well-established association with risky sexual activities. However, only a limited number of studies have explored possible reasons for this association among middle school students, a population that may engage in premarital sex, which is critical for the development of interventions to prevent risky sexual behavior. Based on random samples of middle school students from a Chinese city, this article investigated the relationship between CSA and students’ premarital sexual permissiveness (PSP). Methods In a cross-sectional study conducted between 2016 and 2017 in Luzhou, China, 2292 middle school and high school students aged 12–18 years were recruited by multistage random sampling. All students were administered anonymous questionnaires. Multiple linear regression and binary logistic regression analyses were conducted to analyze the relationship between CSA and PSP. Results The prevalence of CSA was 15.4% (354/2292; 95% CI: 14–16.9%). A higher percentage of male respondents (18.2%) than female respondents (12.9%) had experienced CSA. A positive association between CSA and PSP was found among students. Respondents who had suffered CSA exhibited greater PSP, and this relationship was observed in the male sample, female sample and the total student sample in Luzhou (β = − 3.76, P

Ecological green development is the development of conforming to nature and promoting the harmonious coexistence of man and nature. As the most basic and supportive grassroots political unit, the county level occupies a special key position in the overall national green development strategy. How to evaluate the ecological green development of the county, protect the green earth, and respond to climate change, have become a new problem facing various countries around the world. To this end, China is determined to implement the establishment of a national key ecological functional zone pilot policy to study its ecological green development from the perspective of the county. Based on the literature review and theoretical mechanism, this paper selects 1997 county-level data indicators in mainland China from 2007 to 2019 to explore the role of the establishment of national key ecological function areas on the ecological green development of counties. The implementation of the national key ecological function zone policy can significantly increase the vegetation normalization index by about 0.035–0.037 units. The implementation of the policy has greatly expanded the capacity and range of above-ground and below-ground biomass. The reduction of large-scale investment by the government, the improvement of quality and efficiency of enterprises in the region, and the increase artificial afforestation are important transmission mechanisms for the implementation of policies to affect the green development of counties. The implementation of the policy has a positive green spillover effect on adjacent areas, which can realize regional coordinated ecological governance. Each additional hectare of artificial afforestation can absorb about 134,800 tons of carbon emissions each year, bringing additional benefits of 730 million yuan. It releases about 98,000 tons of oxygen a year, which can be used for 1.312 billion people for oxygen respiration consumption every year.

Objective: We aimed to explore a new method to reverse early relapse in patients with AML1-ETO–positive acute myeloid cell transplantation.Methods: A chidamide-based 3-drug combination regimen was used in our center to treat patients with AML1-ETO–positive AML post transplantation but negative flow cytometry results. A retrospective analysis was performed of the survival rate and possible influencing factors of patients with relapse treated with this regimen in our center from January 2018 to January 2022.Results: The overall response rate was 95.8% (23/24), and the median number of treatment courses was 4 (range, 3–12 courses). The total molecular complete response (MCR) was 79.1% (19/24) after all treatments, and the molecular complete response was 37.5% (9/24) after one cycle of treatment but reached 58.3% (14/24) after four cycles; overall, the proportion of MCR increased gradually with the increase in treatment cycles. The projected 5-year overall survival rate was 73.9%. The projected 5-year leukemia-free survival rate was 64.8%, and the projected 1-year cumulative relapse rate was 35.5%. The incidence of grade II–IV graft-versus-host diseases (GVHD) was 29.2% (7/24), and that of grade III–IV GVHD was 20.8% (5/24), which could be effectively controlled by glucocorticoid therapy combined with calcineurin inhibitors The total incidence of chronic GVHD was 29.2% (7/24), and all cases were localized chronic GVHD. The total infection rate was 33.3% (8/24), mainly involving bacterial and fungal infections, and the incidence of life-threatening infections was 4.17% (1/24). The treatment-related mortality rate was 0%; and the total mortality rate was 20.8% (5/24). Nausea and vomiting, thrombocytopenia, and neutropenia were common adverse reactions, all of which were Common Terminology Criteria for Adverse Events grade 2–3 events and reversible after drug withdrawal. In terms of immunity, Th1 cell counts gradually increased, Th17 cell counts gradually decreased, and the Th1/Th17 ratio gradually increased after treatment. The CD8+ T lymphocyte count increased gradually, while the CD4+ T lymphocyte count did not change significantly.Conclusion: Our chidamide-based 3-drug combination regimen led to a high remission rate and tolerable adverse reactions in patients with AML1-ETO–positive post-transplant relapse, and most patients can achieve long-term survival with this regimen.

Currently, the most direct and effective way to obtain cloud precipitation microphysical characteristics is from in-situ measurements acquired by airborne imaging probes. There are many studies based on CIP (cloud imaging probe) and PIP (precipitation imaging probe) detection data, which are mostly based on the limited output from the software PADS (Particle Analysis and Display System) provided by DMT (Droplet Measurement Technologies). Since PADS only outputs the second-by-second statistical results rather than the detailed particle-by-particle information, it greatly limits the deep mining and analyzing of cloud particle image data. Besides, particle shapes in previous studies are mainly classified through naked-eye observations, which is time consuming, subjective, and unreliable to conduct statistical analysis on thousands of cloud particle images. Therefore, it is impossible to calculate the hydrometeor content based on mass-dimension relationships for particles of different shapes in ice or mixed cloud observations.The operation principle of airborne two-dimensional optical array probes is introduced. Then techniques of recognition and elimination of shattering particles and fake particles are illustrated in detail. Particle shapes are divided into 8 types (tiny, linear, aggregated, graupel, spherical, plate, dendritic and irregular) based on geometric characteristics of particle shapes. Statistical characteristics of different cloud particle shapes and their areas are analyzed by using gray CIP data detected in three wintertime stratiform clouds in Henan Province. The recognition of particle shapes are basically consistent with results through naked-eye observations, and also consistent with dominant particle shapes in each temperature range obtained by previous studies. The hydrometeor content obtained using the mass-dimension relationship for particles of different shapes is compared with that from treating all particles as spherical liquid particles (i.e., the algorithm used by PADS). It is found that when all particles are treated as spherical liquid particles, the hydrometeor content is roughly one magnitude higher than that from considering different particle shapes, indicating the technique of particle shape classification can improve the accuracy of hydrometeor content in ice or mixed clouds. In addition, some matters needing attention in the use of two-dimensional particle image data are pointed out to ensure proper use of two-dimensional particle image data.

ObjectiveTo explore the possible mechanism of Yunaconitine poisoning by studying the changes of urine metabolic profile in rats chronically poisoned by Yunaconitine via non-targeted metabolomics.MethodsA rat model of Yunaconitine poisoning was established, and a metabolomics method based on UPLC-QTOF-MS technology was used to obtain the urine metabolic profile. Principal component analysis （PCA）, orthogonal projections to latent structures-discriminant analysis （OPLS-DA）, variable importance in projection （VIP） value greater than 1, fold change （FC） value greater than 3 or less than 0.33 and P value less than 0.05 were used to screen potential biomarkers related to the toxicity of Yunaconitine. The metabolic pathway analysis was performed through the MetaboAnalyst website and pathological changes of related tissues were observed.ResultsSixteen potential biomarkers including L-isoleucine were screened, which mainly involved six metabolic pathways including the biosynthesis and degradation of valine, leucine and isoleucine, pentose and glucuronate interconversions, and propanoate metabolism, alanine, aspartate and glutamate metabolism, tyrosine metabolism. Pathological studies showed that rat toxic change in nervous system, liver and cardiac caused by Yunaconitine.ConclusionYunaconitine may cause neurotoxicity, hepatotoxicity and cardiotoxicity by affecting amino acid and glucose metabolism.

For both New Zealand and China, agriculture is integral to the economy, supporting primary production in both intensive and extensive farming systems. Grasslands have important ecosystem and biodiversity functions, as well providing valuable grazing for livestock. However, production and persistence of grassland and forage species (e.g. alfalfa) is not only compromised by overgrazing, climate change and habitat fragmentation, but from a range of pests and diseases, which impose considerable costs on growers in lost production and income. Some of these pest species are native, but increasingly, international trade is seeing the rapid spread of exotic and invasive species. New Zealand and China are major trading partners with significant tourist flow between the two countries. This overview examines the importance of grasslands and alfalfa in both countries, the current knowledge on the associated insect pest complex and biocontrol options. Identifying similarities and contrasts in biology and impacts along with some prediction on the impact of invasive insect species, especially under climate change, are possible. However, it is suggested that coordinated longitudinal ecological research, carried out in both countries using sentinel grass and forage species, is critical to addressing gaps in our knowledge of biology and impact of potential pests, along with identifying opportunities for control, particularly using plant resistance or biological control.

A system failure probability analysis method of cohesive slope considering the spatial variability of undrained shear strength is proposed. In this method, the local averaging parameter of the random field of undrained shear strength on slip surface is introduced as an equivalent parameter. The statistical characters of the equivalent parameter and the correlation coefficient between different equivalent parameters are formulated. Then, the reliability index of a single failure mode and the correlation coefficient between different failure modes are calculated based on the equivalent parameters. By considering both the reliability index and correlation coefficient between different failure modes, the representative slip surfaces are searched step by step, and the system failure probability is assessed using those representative slip surfaces. Finally, to verify this method, three slopes are analyzed as examples. The results show that the equivalent parameter obtained by local averaging along the circular slip surface is feasible to describe the spatial variability of the undrained shear strength, and the proposed method can assess the system failure probability of cohesive slope with small error. Meanwhile, the correlation coefficient between failure modes increases with the spatial correlation of the random field, thus the number of representative slip surfaces required to achieve convergence will reduce.

In today’s society, with a high percentage of elderly people, floor heating to ensure constant temperature and heat jackets in winter play important roles in winter to them to live comfortable lives without compromising health – except tropical zones. Under floor heating maintains a comfortable temperature in a room without polluting the air and heat jackets allow for light clothing at comfortable temperatures. The two facilities are attributed to Joule heat generated by tunnel currents between adjacent short carbon fillers in flexible polymer matrixes under low voltage. The current between adjacent conductive fillers is due to electron transfer associated with elementary quantum mechanics. Most of undergraduate students investigating polymer physics will have learned about electron transfer in relation to the temperature dependence of the conductivity of conductive filler-insulator polymer composites as well as the phenomenon of Joule heat at high school. Despite their industrial importance, most students show little interest for investigating electric properties, since most of polymers are insulation materials. Polymer scientists have carried out qualitative analyses for tunneling current using well-known simplified equations derived from complicated mathematical process formulated by solid-state physicists. Hence this paper is focused on a teaching approach for temperature dependence on electric properties of the polymer-filler composites relating to tunnel current in terms of elementary quantum mechanics. The approach also attempts to bridge education and research by including reference to the application limit of the well-known theories to such complicated composite systems that fillers are dispersed uniformly in the polymer matrix.

C51H79O11Y, monoclinic, P21/c (no. 14), a = 8.8682(3) Å, b = 22.1328(8) Å, c = 27.9848(10) Å, β = 96.710(1)°, V = 5455.2(3) Å3, Z = 4, Rgt(F) = 0.0495, wRref(F2) = 0.1275, T = 296(2) K.

Wind Turbine Gearbox is an important part of the wind turbine,in the long-term operation will inevitably occur various types of faults,its inspection and maintenance methods will become the focus of scholars. By analyzing the research status of condition monitoring methods and means,fault feature extraction methods and maintenance strategies for wind turbine gearbox,the main problems in the research of inspection and maintenance methods are summarized. Some suggestions are put forward,such as the research of on-line monitoring method and means of wind turbine gearbox based on multi-sensor,the research of intelligent fault diagnosis method of wind turbine gearbox based on multi-source information fusion,the research of preventive maintenance strategy of wind turbine gearbox based on life distribution and real-time monitoring data,etc. It can be used for reference for the research of wind turbine gearbox inspection and maintenance method.