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The paper pointed out that chain network topology is suitable for underground wireless Mesh network and electromagnetic wave attenuation analysis and node space design should be considered combining with concrete roadway environment according to characteristics of underground roadway. It proposed a method combining reactive routing protocol and proactive routing protocol with MIC criterion for that current routing protocol cannot meet with requirements of chain network topology of underground wireless Mesh network. It gave general structure and characteristics of coal mine emergency rescue system based on wireless Mesh network on the above basis. The experiment result showed that applying wireless Mesh network in coal mine emergency rescue system can improve safety, reliability and feasibility of rescue work.

In this paper, the exciton binding energy and radiation lifetime in type I and II structures of CdTe/Cd 1 − x ZnxS strained core/shell spherical quantum dots under the hydrostatic pressure have been studied by using the variational method within the continuous dielectric model and effective mass approximation. The results show that for these two structures, the exciton binding energies with and without strain decrease with the increasing core radius but increase with the increasing pressure. The exciton binding energy with strain is smaller than that without strain. For type I structure, the effect of strain is small, and the radiation lifetime decreases monotonically with the increasing pressure. By contrast, for type II structure, the effects of shell radius and strain on the exciton binding energy are obvious, and the radiation lifetime increases first and then decreases with the increasing pressure. [ABSTRACT FROM AUTHOR]

Underwater Wireless Optical Communication (UWOC) has the advantages of large capacity, high speed and etc. However, it suffers from limited communication range and data rate in practice due to the complex underwater environment and the limited bandwidth of optical devices. Multiple-Input Multiple-Output (MIMO) technology has received considerable attention since it converts spatial single-stream data into multiple-stream data for parallel transmission. Therefore it can extend the UWOC range and alleviate the performance degradation caused by underwater absorption, scattering, and turbulence. In this paper, focusing on the MIMO technology in UWOC systems, we analyzed the channel modeling, research progress, experimental setups and prototypes, and the development and future trend of MIMO-UWOC technology.

Objective To study the effect of miR-499a-5p on injury induced by chemical hypoxia of pheochromocytoma cell(PC12). Methods PC12 cells were divided into control group, hypoxia group (treated with CoCl2 for 24 h), hypoxia+miR-NC or miR-mimic groups. Cell viability was detected by MTT assay; the expression of miR-499a-5p was detected by RT-qPCR; lactate dehydrogenase (LDH) activity was detected by kit; apoptosis was detected by flow cytometry; the expression of phosphatase and tensin homolog (PTEN) protein was detected by Western blot. The luciferase reporter gene was used to detect whether Pten was a direct target of miR-499a-5p. Results Compared with control group, the cell viability and miR-499a-5p expression decreased (P＜0.01 or P＜0.001), LDH activity and apoptosis increased (P＜0.01 or P＜0.001) in hypoxia group. Over-expression of miR-499a-5p enhanced survival rate and inhibited apoptosis of PC12 cells under hypoxia (P＜0.05 or P＜0.01). Pten was a direct target of miR-499a-5p. Conclusions miR-499a-5p alleviates hypoxic injury of PC12 cells through targeting at Pten.

BACKGROUND: Ovarian cancer (OC) is the fifth most common type of cancer in women worldwide. MiR-27a plays an important role in the development of ovarian cancer. However, the exact function and molecular mechanism of miR-27a in epithelial-mesenchymal transition (EMT) has not been thoroughly elucidated to date. METHODS: Quantitative real-time PCR (qRT-PCR) was used to determine the expression of miR-27a and FOXO1 mRNA in ovarian tissues and cells. The function of miR-27a in ovarian cancer was investigated through overexpression and knockdown of miR-27a in vitro. Wound healing and Transwell assays were performed to evaluate the migration and invasive capacity of the cells. A luciferase reporter assay was conducted to confirm the interaction between miR-27a and FOXO1. Western blotting was used to evaluate FOXO1, EMT and Wnt/ β -catenin relative protein expression. RESULTS: In our study, we found that the mRNA expression level of miR-27a was significantly higher in ovarian cancer tissues and in HO8910 and OV90 cells. Functional experiments showed that miR-27a overexpression potentiated the migration and invasion of HO8910 and OV90 cells, while miR-27a inhibition reduced the cells' migration and invasion. Moreover, miR-27a upregulated the expression of mesenchymal cell markers and downregulated the expression of epithelial cell markers, which were restored via silencing of miR-27a expression. Subsequently, miR-27a was found to directly target and suppress the expression of FOXO1. Finally, we demonstrated that miR-27a promoted the progression of ovarian cancer cells and induced the process of EMT via the Wnt/ β -catenin signalling pathway through inhibition of FOXO1. CONCLUSIONS: Taken together, these results indicate that targeting miR-27a and FOXO1 could represent a strategy for anticancer therapy in ovarian cancer. [ABSTRACT FROM AUTHOR]

Increasing occurrence of posterior reversible encephalopathy syndrome has been reported in children with acute lymphoblastic leukemia. However, the etiology of posterior reversible encephalopathy syndrome is not clear. To study the possible pathogenetic mechanisms and treatment of this complication, we reported 11 cases of pediatric acute lymphoblastic leukemia who developed posterior reversible encephalopathy syndrome after induction chemotherapy. After appropriate treatment, the clinical symptoms of posterior reversible encephalopathy syndrome in most cases disappeared even though induction chemotherapy continued. During the 1-year follow-up, no recurrence of posterior reversible encephalopathy syndrome was observed. Although the clinical and imaging features of posterior reversible encephalopathy syndrome may be diverse, posterior reversible encephalopathy syndrome should be recognized as a possible important complication of acute lymphoblastic leukemia when neurologic symptoms appear. In line with previous reports, our study also indicated that posterior reversible encephalopathy syndrome was reversible when diagnosed and treated at an early stage. Thus, the occurrence of posterior reversible encephalopathy syndrome should be considered and investigated to optimize the early induction scheme of acute lymphoblastic leukemia treatment. [ABSTRACT FROM AUTHOR]

The key issue of the purpose of detecting target vehicle speed based on video is how to obtain the accurate and efficient real-time information of moving targets using the video sequence in unstructured environment. Vehicle speed detection based on visual perception mechanism is a kind of new method. Visual mechanism inspired the method, first of all it should learn the over-complete characterization of primary visual cortex cells from nature images. Then it compared with the dynamic threshold to identify the moving target based on over-complete visual calculation model according to neuron response coefficient characterization and the difference of neuronal response coefficient between background images and goal images. Next, it achieved target tracking through the iterative method. Compared with the traditional method it got the displacement of the vehicle according to the matching method of characteristics in different video sequences on the basis of target tracking. Finally it could realize speed detection by this algorithm. Experimental results show that this algorithm can test the vehicle speed of different types in the actual road scene under real-time conditions and reach more than 96.55% accuracy. The algorithm improves the vehicle speed detection in accuracy and robustness compared with the traditional method. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of molar erection on periodontal tissue by measuring the levels of interleukin-1β, IL-6 and IL-8 in gingival crevicular fluid (GCF) during the orthodontic treatment. Methods 30 slanted molars were erected. During the treatment, the clinical parameters including bleeding index (BI), gingival index (GI) and gingival crevice depth (GCD) were detected at the point of pre-erecting, 1-week after beginning treatment, finishing treatment and 1-week after finishing treatment. GCF was also collected from buccal and lingual of medial site and distal site at each time point and the level of IL-1β, IL-6 and IL-8 were detected. Results At the point of the finishing treatment, GI, GCD and all GCF indexes showed significant differences as compared to 1-week after beginning treatment. And at the point of 1-week after finishing treatment, all indexes showed significant differences as compared to pre-erecting. Conclusion Erecting the molar has good effect on the periodontal tissue. [ABSTRACT FROM AUTHOR]

Abstract Background Accumulating evidence reveals that intrauterine growth retardation (IUGR) can cause varying degrees of pulmonary arterial hypertension (PAH) later in life. Moreover, epigenetics plays an important role in the fetal origin of adult disease. The goal of this study was to investigate the role of epigenetics in the development of PAH following IUGR. Methods The IUGR rats were established by maternal undernutrition during pregnancy. Pulmonary vascular endothelial cells (PVEC) were isolated from the rat lungs by magnetic-activated cell sorting (MACS). We investigated epigenetic regulation of the endothelin-1 (ET-1) gene in PVEC of 1-day and 6-week IUGR rats, and response of IUGR rats to hypoxia. Results The maternal nutrient restriction increased the histone acetylation and hypoxia inducible factor-1α (HIF-1α) binding levels in the ET-1 gene promoter of PVEC in IUGR newborn rats, and continued up to 6 weeks after birth. These epigenetic changes could result in an IUGR rat being highly sensitive to hypoxia later in life, causing more significant PAH or pulmonary vascular remodeling. Conclusions These findings suggest that epigenetics is closely associated with the development of hypoxic PAH following IUGR, further providing a new insight for improved prevention and treatment of IUGR-related PAH.

Only one dimensional displacement in the line of sight (LoS) can be detected using DInSAR technique. In order to overcome this limitation, the three-dimensional (3-D) model and algorithm which fusing multi-source SAR images acquired with different geometric parameters in different satellite platform was proposed and validated to determine the 3-D displacement due to underground mining. Comparing with leveling observation indicates that the displacement derives from the 3-D algorithm coincide with leveling data more closely, and the root mean square error (RMSE) is ±4 mm. May be due to the activation of old goafs, both the displacement and contour in vertical direction indicates that the subsidence basin extends to the old goaf. The displacement and contour in east-west direction accord with the law of surface movement caused by mining, and the effects on the displacement along LoS will be significantly different when imaging mode different. Due to the small sine value of heading angle, the 3-D algorithm is not sensitive to the displacement in the north-south direction. [ABSTRACT FROM AUTHOR]

On the basis of analyzing the mechanism of residual subsidence of old goaf, the model of calculating residual subsidence of gobs was deduced by combining the results of ruptured rock mass. Meanwhile the relationship were analyzed between the coefficient of residual subsidence and the characteristic of overburden strata, mining thickness, mining depth and building load. The research results show that while mining thickness is same, the shallower mining depth is, the larger coefficient of residual subsidence is, and there is larger surface residual subsidence in the future; While mining depth is same, the larger mining thickness is, the smaller coefficient of residual subsidence is ; When the propotion of constuction loads to overburden stress is the same,the smaller the mining depth is,the larger the augmenter of subsidence coefficient is, and has more effect old goafs. [ABSTRACT FROM AUTHOR]

Background: To investigate the clinical and neuroimaging characteristics of leukoencephalopathy among children with acute lymphoblastic leukemia (ALL), especially after chemotherapy., Methods: Clinical data for 17 pediatric patients with leukoencephalopathy and 17 matched controls were retrospectively analyzed. All participants were children with ALL admitted to the Children's Hospital of Soochow University from May 2011 to April 2021. The data mainly consisted of general information, laboratory studies, and imaging diagnostic results., Results: Overall, 94.12% of the patients experienced neurological symptoms. The most common symptoms were seizure (7/17, 41.18%), nausea (5/17, 29.41%), vomiting (5/17, 29.41%), paralysis (5/17, 29.41%), and numbness (4/17, 23.53%). On neuroimaging, multiple and irregular lesions were observed, distributed mainly in the periventricular area (9/17, 52.94%), parietal lobe (6/17, 35.29%), and basal ganglia (5/17, 29.41%). Moreover, there were significant differences in serum sodium (P=0.0001), C-reactive protein (P=0.0124) and blood pressure (P=0.0271) between patients with and without leukoencephalopathy. After aggressive treatment, the clinical symptoms (12/17, 70.59%) and imaging lesions (11/13, 84.62%) gradually improved in most patients., Conclusions: Chemotherapy is an important risk factor related to leukoencephalopathy. Although the clinical symptoms of leukoencephalopathy vary widely, there is a high degree of consistency in its radiological features. Abnormal laboratory results may also help the identification of leukoencephalopathy. Early detection and treatment can improve brain development in the long term., Competing Interests: Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at https://tcr.amegroups.com/article/view/10.21037/tcr-22-2180/coif). The authors have no conflicts of interest to declare., (2023 Translational Cancer Research. All rights reserved.)

Objective: To investigate the recurrence rate and risk factors of spontaneous symptomatic epileptic seizures after the first episode in infants and young children., Methods: The clinical data of infants and young children who experienced the first episode of spontaneous symptomatic epileptic seizures between April 2009 and April 2011 in Suzhou Children's Hospital were collected. Follow-up visits were performed once every 1-3 months, and the follow-up time was 1-60 months. The Kaplan-Meier method and Cox proportional hazards model were applied to calculate the recurrence rate of spontaneous symptomatic epileptic seizures and analyze the risk factors for seizure recurrence., Results: Sixty-three children experiencing a first episode of spontaneous symptomatic epileptic seizures were enrolled. Within 5 years after the first episode, 43 children experienced the recurrence of spontaneous symptomatic epileptic seizures, with a 5-year cumulative recurrence rate of 69.4%. Among all recurrent cases, 86% experienced recurrence within 1 year after the first episode. The multivariate analysis with the Cox proportional hazards model showed that epileptiform discharges on electroencephalography were the independent risk factor for recurrence of spontaneous symptomatic epileptic seizures (HR=5.349, 95%CI: 2.375-12.048)., Conclusions: The recurrence rate of spontaneous symptomatic epileptic seizures after the first episode is high in infants and young children. Epileptiform discharges on electroencephalography are the independent risk factor for the recurrence, and thus it is suggested to perform antiepileptic therapy for these children.

Objective: To study the diagnostic values of cerebrospinal concentrations of neopterin (NPT) and S100b for central nervous system infections in children., Methods: Enzyme-linked immunosorbent assay was used to determinate the cerebrospinal concentrations of NPT and S100b in children with central nervous system infections and control children. The two groups of children were compared in terms of the two indicators, and the diagnostic values of the two indicators were evaluated by ROC curve analysis., Results: Children with viral encephalitis had significantly increased cerebrospinal concentrations of NPT and S100b compared with the control group and children with purulent meningitis (P<0.01); there was no difference in the cerebrospinal concentration of NPT between children with purulent meningitis and the control group, while the concentration of S100b in the purulent meningitis group was significantly higher than in the control group (P<0.01). According to the ROC curves, S100b was more valuable than NPT in the diagnosis of viral encephalitis; when cerebrospinal concentration was more than 0.384 ng/mL, S100b had a sensitivity of 93.3% and a specificity of 97.9%; a combination of the two indicators had a higher diagnostic value for viral encephalitis, with a sensitivity of 96.7% and a specificity of 97.9%., Conclusions: Both NPT and S100b have certain values in the diagnosis of central nervous system infections in children, and S100b is better than NPT.

Objective: To investigate the correlation of sperm DNA damage and sperm-nucleoprotein transition with acrosin activity and seminal parameters., Methods: We collected 535 semen samples, assessed sperm DNA damage by sperm chromatin dispersion test, and analyzed the correlation of sperm DNA damage and sperm-nucleoprotein transition with acrosin activity and seminal parameters according to the WHO criteria., Results: Statistically significant differences were observed in sperm DNA damage among sperm-nucleoprotein transition, acrosin activity, sperm concentration and the percentage of grade a + b sperm (P < 0.01). Sperm DNA damage was positively correlated with age, sperm-nucleoprotein transition, sperm concentration and the percentage of grade d sperm (P < 0.01 or P < 0.05), but negatively correlated with acrosin activity (P < 0.001). Stepwise linear regression analysis demonstrated that age, sperm concentration, the percentage of grade d sperm, sperm-nucleoprotein transition and acrosin activity were independent variables related to the DNA fragmentation index (DFI). The abnormality rates of sperm-nucleoprotein transition, acrosin activity, sperm concentration and graded a + b sperm were significantly higher in the sperm DNA damage group (DFI > or = 30%) than in the normal control (DFI < 30%) (P < 0.01)., Conclusion: Sperm DNA damage is closely related with sperm-nucleoprotein transition, acrosin activity and seminal parameters, which may become another important independent parameter for the evaluation of sperm quality.

Objective: To investigate the antitumor effect of natural killer (NK) cells on human colorectal cancer cells HT-29 in vitro by blocking transforming growth factor-β (TGF-β) signaling in NK cells transfected with vector containing dominant negative TGF-β type 2 receptor (DNTβR2)., Methods: TGF-β1 was added at the final concentration of 10 ng/ml for HT-29 cells. Primary NK cells were transfected with recombinant plasmid pIRES2-AcGFP-DNTβR2 and control plasmid pIRES2-AcGFP using Amaxa Nucleofector technology respectively. The cytotoxicity of these two types of NK cells to HT-29 cells was detected and analyzed by cell counting kit-8., Results: The transfection efficiency of primary NK cells was 18.85% for the plasmid pIRES2-AcGFP-DNTβR2 and 35.28% for the control plasmid pIRES2-AcGFP. The expression of DNTβR2 in NK cells was confirmed by Western blotting and RT-PCR. Primary NK cells displayed significantly lower cytotoxicity against HT-29 cells incubated with TGF-β1 than that without TGF-β1 (effect-target cell ratio 10:1,14.40%∓ 2.00% vs. 26.14% ∓ 2.50%, P > 0.05; effect-target cell ratio 20:1, 19.18% ∓ 2.49% vs. 40.81% ∓ 3.50%, P > 0.05). The cytotoxicity of NK cells transfected with DNTβR2 vector was significantly higher than that with control vector against HT-29 cells cultured with 10 ng/ml TGF-β1 (effect-target cell ratio 10:1, 21.17% ∓ 2.49% vs. 11.48% ∓ 1.11% ,P > 0.05; and effect-target cell ratio 20:1, 35.30% ∓ 3.78% vs. 17.19% ∓ 2.29%, P > 0.05)., Conclusion: NK cells transfected with DNTβR2 vector show better antitumor effect, which may provide new method for NK-based adoptive immunotherapy for cancer.

In the title compound, C(9)H(11)N(3)O(2)S, intra-molecular O-H⋯O and N-H⋯N hydrogen bonds contribute to the planarity of the mol-ecular skeleton. Inter-molecular N-H⋯O hydrogen bonds link the mol-ecules into zigzag chains along the b axis; these mol-ecules are futher paired by π-π inter-actions [centroid-centroid distance 4.495 (5) Å]. The crystal structure also exhibits weak inter-molecular N-H⋯S and O-H⋯S hydrogen bonds.

Objective: To study the relationship between vulvovaginitis in pre-pubertal girls and pathogens as Chlamydia trachomatis (Ct), N. gonorrhoeae (Ng), Mycoplasma, Ureaplasma urealyticum (Uu), Mycoplasma hominis (Mh), M. genitalium (Mg), M. fermentans (Mf) and M. penetrans (Mpe), as well as to find out the proportion of mycoplasma which is correlated to sexually transmitted diseases (STD) and AIDS. METHODS Vulvae swab specimens from 285 pre-pubertal girls with vulvovaginitis (case group) and 128 healthy girls (control group) were collected and detected by nested polymerase chain reaction (nPCR) to identify the existence of pathogens as Ct, Ng, Uu, Mh, Mg, Mf and Mpe. nPCR with both high specificity and sensitivity, would not be influenced by the amount of pathogens in specimens or inactivated during the process of storage or transportation., Results: The rate of detection on pathogens was 59.65% in the 285 specimens from case group including 'one kind of pathogen in one specimen' as 37.54% and 'two kinds' as 16.84% and 'three kinds' as 5.26%. However, in the 128 specimens from control group, the detectable rate of pathogen was 6.25%. Relationships were found between Ng (P < 0.01), Ct (P < 0.01), Uu (P < 0.01), Mg (P < 0.01), Mf (P < 0.05), Mpe (P < 0.01) and vulvovaginitis in pre-pubertal girls. In control group the pathogens were detected from 7 specimens including 5 Uu and 2 Mh., Conclusion: Some of the pathogens were correlated to STD and were important in causing vulvovaginitis in pre-pubertal girls. Vulvovaginitis might have been caused by more than one kind of pathogen in pre-pubertal girls. The locations of Mg, Mf and Ng in outer genital tracts were correlated to seasonal change. Macrolide seemed to be quite effective clinically in treating urogenital tract infection caused by mycoplasma and Ct.

Objective: To analyze the meiotic segregation results of male reciprocal chromosome translocation by fluorescence in situ hybridization (FISH)., Methods: Multi-color FISH using 3 combined probes located in any 3 chromosome segments on both sides of two breakpoints was performed on the de-condensed sperm head to analyze the sperm chromosomal contents and segregation patterns., Results: Four male reciprocal translocation carriers were included in the study, with the karyotypes of 46, XY, t(2;18) (p16; q23); 46, XY, t(4;6) (q34;q21); 46, XY, t(8;13) (q23;q21) and 46, XY, t(4;5) (4q31;5q13), respectively. The results showed that 4 carriers had different proportions of various segregated spermatozoa. The spermatozoa of alternate, adjacent-1, adjacent-2, 3:1, non-disjunction in meiosis II, and 4:0 or diploidy accounted for 27.1%-49.4%, 26.9%-37.6%, 2.7%-15.7%, 8.6%-32.7%, 0.2%-1.9%, and 0.1%-0.4%, respectively., Conclusion: For each-reciprocal translocation carrier seems to have a particular meiotic segregation results, FISH analysis on sperm head should be done for each carrier in order to provide an accurate genetic counseling.

Objective: The present study aimed to clone and express three fragments of genomic RNA derived from SARS associated coronavirus (SARS-CoV) S1 domain and to study its immunogenicity., Methods: The S1 domain gene was amplified by PCR with specific primers and was inserted into the prokaryotic expression vector pQE-30. Three fragments (40-751, 746-1344 and 746-2001 bp) derived from S1 domain produced after the recombinant plasmid (pQE-30/S1) was digested by restriction endonucleases. The three fragments were cloned into pQE-30 and expressed in M15 strains of Escherichia coli. The expression products, designated S1a, S1b and S1c respectively, were purified by Ni affinity chromatography. The immunogenicity was analyzed by Western Blot and ELISA using serologically confirmed sera from SARS patients and the sera from healthy donors was used as control at the same assay., Results: Three recombinant plasmids (pQE-30/S1a, pQE-30/S1b, pQE-30/S1c) were constructed.Fusion proteins with relative molecular mass of 26,700, 22,500 and 46,000 dalton were successfully expressed with amounts of 35%, 35% and 30% of total cell protein and purified by Ni affinity chromatography, respectively. Western Blot and ELISA analysis showed that the S1c protein could be specifically recognized by the sera from SARS patients., Conclusion: The recombinant S1c protein was a good immunogen and has the potential to be used as a vaccine against SARS-CoV infection.

Objective: To develop a rapid and efficient method for preparing monoclonal antibodies (mAb) against SARS-associated coronavirus (SARS-Cov) nucleocapsid (N) protein., Methods: BALB/c mice were injected with the recombinant N protein of SARS-Cov into the foot-pads for the immunization, and the popliteal lymph nodes were isolated 15 d later for mAb-producing hybridomas, from which the mAbs against the N protein of SARS-Cov were screened. The identification of the mAb against the N protein of SARS-Cov was performed using indirect enzyme-linked immunosorbent assay (ELISA), indirect fluorescent-antibody assay (IFA), and Western immunoblotting., Results: Four strains of hybridomas were obtained that produced the mAb specific to the N protein without detectable cross-reactivity with other pathogens. Of the 4 strains, 2 were identified as the immunoglobulin G1 (IgG1) isotype, 1 IgG2a, and the other IgG2b, with affinity constants (Ka) of 2 of the strains being 4.14 x 10(-9)M and 3.19 x 10(-9)M respectively., Conclusion: This is the first report on the preparation of mAb that is specific to the SARS-Cov, and the high-specificity and high-affinity mAb produced by the 4 strains of hybridomas provide a basis for further researches on the pathogenesis and early diagnosis of SARS.