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This paper details the creation of a human gross anatomy laboratory from a defunct chemistry laboratory at West Liberty University, a small primarily undergraduate institution in West Virginia. The article highlights important considerations with regard to the development of a human gross anatomy laboratory including access to human gifts; assessment of the space for size, security, and privacy; assessment of the utilities; acquisition of a dissection table; ventilation; aesthetics in functional design; expenses; and sustainability. The report also identifies favorable conditions and potential pitfalls regarding the creation of a human gross anatomy laboratory. This paper demonstrates that a human gross anatomy laboratory can be created quickly and at little expense.

The preparation and processing of rodent brains for evaluation by immunohistochemistry is time-consuming. A large number of mouse brains are routinely used in experiments in neuroscience laboratories to evaluate several models of human diseases. Thus, methods are needed to reduce the time associated with processing brains for histology. A scalable method was developed to embed, section, and stain multiple mouse brains using supplies found in any common histology laboratory. Section collection schemes can be scaled to provide identical bregma locations between adjacent sections for immunohistochemistry, facilitating comprehensive, high-quality immunohistochemistry. As a result, sectioning and staining times are considerably reduced as sections from multiple blocks are stained simultaneously. This method improves on previous procedures and allows multiple embedding and subsequent immunostaining of brains easily with a dramatically reduced time requirement. Furthermore, we expand this method for use in numerous mouse tissues, rat brain tissue, and post-mortem human brain and arterial tissues. In summary, this procedure allows the processing of many rodent or human tissues from perfusion through microscopy in 10 days or less. [ABSTRACT FROM AUTHOR]

Introduction/Background: Anterior clinoidectomy is a routine neurosurgical procedure for the management of paraclinoid aneurysms and tumors in adults; however, it has been performed less extensively in children. The anterior clinoid process of the sphenoid bone is closely related to vital neurovascular structures including the optic nerve, internal carotid artery, and the ophthalmic artery. Therefore, sphenoidal variants, such as the accessory optic canal and caroticoclinoid foramen, pose serious complications in anterior clinoidectomy including potential blindness and death. Case presentation: This report presents a novel case of concomitant bilateral accessory optic canals and a right-sided caroticoclinoid foramen found within an infantile sphenoid of a 12-month-old black male. The case report documents two co-occurring, clinically relevant variants known to complicate anterior clinoidectomy in an individual from a population that has been underrepresented in the literature. Conclusions: Caroticoclinoid foramina and accessory optic canals can be mistaken for each other on preoperative imaging, and accessory optic canals can additionally be mistaken for pneumatization of the anterior clinoid process, lesser sphenoidal wing, or optic strut. A high index of suspicion for these anatomic variants on preoperative imaging could enable the prevention of adverse outcomes, including hemorrhaging of the internal carotid artery and/or ophthalmic artery and subsequent blindness or death. [ABSTRACT FROM AUTHOR]

Introduction: Bronchoalveolar lavage (BAL) is frequently used in pulmonary medicine though it requires further optimization. Practical obstacles such as patient safety and procedural limitation have to date precluded large, controlled trials aimed at standardization of BAL procedure. Indeed, BAL guidelines are based on observational data. Innovative research methods are necessary to advance the clinical practice of BAL. Methods: In our study, we evaluated the effect of injecting a gelatinized barium solution into different lobes and segments of cadaveric lungs. As the technique requires an irreversible injection into lung airspaces, it is not suitable for in vivo purposes. We measured the volume returned from BAL as well as the distribution of BAL injection via dissection. Segmental anatomic orientation was compared to a radiologist's impression of plain film radiographs taken of injected lungs. Results: Mean injected volume distributions were greatest in the upper lobes and lowest in the lower lobes; mean ratios of injected volume distribution to lung lobe volume also followed this trend. Cannulated bronchi orders favored lower branches in the upper lobe and higher branches in the lower lobes. Segmental anatomy varied by the lung lobe injected and was most varied in the lower lobes. Conclusion: This novel gelatinized‐barium injection technique provides a minimally complex method to yield clinically meaningful feedback on the performance of BAL. The technique is also adaptable to study of procedural parameters in the context of variable lung anatomies and pathologies. [ABSTRACT FROM AUTHOR]