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BackgroundThe use of enzymes within pig feed can reduce the challenges associated with antibiotic-free animal feeding. However, this enzymatic effect is often limited by the internal and external gut environment. This study aimed to improve diet quality and assess the impact of an enzymatically hydrolyzed diet (EHD) on growth performance, meat quality, and intestinal health in growing pigs.MethodsThe EHD was prepared by treating a liquid basal diet with a compound enzyme preparation (5 non-starch polysaccharides (NSP) enzymes: cellulase, pectinase, xylanase, β- glucanase, α-galactosidase; 3 exogenous digestive enzymes: amylase, lipase, protease; lysozyme, and glucose oxidase) in a 1:2.5 ratio with water and heated at 40°C for 1 h. Thirty-six growing pigs (average body weight 25 ± 0.5 kg; age 75 ± 3 days) from the Duroc × Landrace × Yorkshire crossbreed were randomly divided into three dietary groups: solid basal diet (S-CON), liquid basal diet (L-CON), and EHD.ResultsEnzymatic pre-treatment reduced the anti-nutritional factors (ANFs) in the diets (p

Abstract In museums, silk cultural relics are highly sensitive to light. However, lighting is inevitable due to the exhibition needs, despite any light would pose irreversible damage to silk. Although the solution of eliminating UV radiation was widely achieved in museum lighting environment, long-time accumulation of other light sources still would induce photodegradation of silk. This work therefore established the simulated light ageing experiments to assess the degradation behaviors of silk samples with different ageing degrees. The variation of color values and structure transformation of each sample group were determined by the means of scanning electron microscopy (SEM), colorimeter, Fourier Transform infrared spectroscopy (FTIR) and 13C CPMAS NMR. The results indicated that silk samples with different initial ageing degrees presented different discoloration tendency and structural variation in experimental lighting environment, suggesting the higher disordered structure makes the silk more vulnerable to light damage. It is of great significance to understand the long-time impacts of illumination on degraded silk and further provide methodology for predicting the duration of exhibited silk cultural relics.

AbstractPseudanthias pascalus (Jordan & Tanaka, 1927) (Perciformes: Serranidae) is a species of brightly colored saltwater fish found in tropical coastal reef communities. In this study, we reported the sequence of mitochondrial DNA from P. pascalus. The accession number is OP611422. The complete mitochondrial genome of P. pascalus was 16,863 bp in length, including 13 protein-coding genes (PCGs), 12S and 16S rRNAs, 22 tRNA genes, and one displacement loop (D-loop). Most PCGs had ATG-start codons and TAA-end codons. The A + T contents were 54.61%. Phylogenetic analysis showed that P. pascalus is most closely related to Pseudanthias huchtii. We sequenced the entire mitochondrial genome of P. pascalus, providing improved marker identification information for the classification of the family and species conservation. These data will be useful for relative ecological and phylogenetic studies.

Abstract Changzhi Guanyin Hall, a folk temple from the Ming Dynasty, embodies the essence of the ‘unity of the three religions’ philosophy, centred around Guanyin Bodhisattva. Over 500 sculptures reside within a modest 70 m2 space, encompassing representations from Confucianism, Buddhism and Taoism. Ranging from a towering 2 m to a mere 10 cm, these sculptures depict an array of characters and mythological scenes, bestowing considerable importance upon the painted sculptures of Guanyin Hall. Varying degrees of deterioration have affected these sculptures due to the passage of time and suboptimal preservation conditions, highlighting the imperative need for meticulous restoration efforts. However, the absence of comprehensive analyses pertaining to the production process of Guanyin Hall sculptures has hindered the restoration groundwork. This study focuses on fallen hanging sculptures and debris, encompassing intact sculptures, clay fragments, wooden sticks, plant fibres, wires and pigments. Employing a series of techniques, including X-ray photography, microscopic analysis, mineral analysis (MLA), laser particle size testing, ion chromatography, X-ray fluorescence spectroscopy (XRF), X-ray diffractometer (XRD) and energy dispersive spectrometer (SEM–EDS) analysis, this research characterises the constituent materials used in crafting these artifacts. Instead of a full-body skeleton, the findings reveal that the small hanging sculpture is supported by delicate wires that hold specific areas, such as the arms and the gown hems. The hanging sculptures are made from a single type of clay and are reinforced with either mulberry bast or wheat straw fibres. A Pinaceae wood stick affixes the head to the body, and the sculpture is then suspended on the wall through nails inserted into the feet. The moulded clay undergoes a sequence of carving, drying and polishing and is coated with a white ash layer before receiving intricate painting. Mineral pigments, including cinnabar, red lead, iron red, cerussite, atacamite, brochantite and azurite, adorn the surface of the sculptures, with gold foil embellishing the golden elements. The intricate artisanry and multifaceted material composition of Guanyin Hall’s painted sculptures render them unparalleled artistic treasures, not solely within Shanxi but across the entire nation. Furthermore, this study establishes a dependable foundation for conservation and restoration endeavours.

Irreversible fibrosis following myocardial infarction (MI) stiffens the infarcted myocardium, which remains challenging to restore. This study aimed to investigate whether the injectable RLP12 hydrogel, derived from recombinant resilin protein, could serve as a vehicle for stem cells to enhance the function of the infarcted myocardium. The RLP12 hydrogel was prepared and injected into the myocardium of rats with MI, and brown adipose-derived mesenchymal stem cells (BADSCs) were loaded. The survival and differentiation of BADSCs in vivo were investigated using immunofluorescence one week and four weeks after treatment, respectively. The heart function, MI area, collagen deposition, and microvessel density were further assessed four weeks after treatment through echocardiography, histology, immunohistochemistry, and immunofluorescence. The RLP12 hydrogel was prepared with a shear modulus of 10–15 kPa. Four weeks after transplantation, the RLP12 hydrogel significantly improved cardiac function by increasing microvessel density and reducing infarct area size and collagen deposition in MI rats. Furthermore, the distribution ratio of collagen III to I increased in both the centre and edge areas of the MI, indicating the improved compliance of the infarct heart. Moreover, the RLP12 hydrogel also promoted the survival and differentiation of BADSCs into cardiac troponin T- and α-smooth muscle-positive cells. The RLP12 hydrogel can be utilised as an injectable vehicle of BADSCs for treating MI and regulating collagen I and III expression profiles to improve the mechanical microenvironment of the infarct site, thereby restoring heart function. The study provides novel insights into the mechanical interactions between the hydrogel and the infarct microenvironment.

Dexamethasone (Dex) is a widely used glucocorticoid in medical practice, with applications ranging from allergies and inflammation to cerebral edema and shock. Despite its therapeutic benefits, Dex is classified as a prohibited substance for athletes due to its potential performance-enhancing effects. Consequently, there is a critical need for a convenient and rapid detection platform to enable prompt and accurate testing of this drug. In this study, we propose a label-free Förster Resonance Energy Transfer (FRET) aptasensor platform for Dex detection utilizing conjugated polymers (CPs), cationic conjugated polymers (CCPs), and gene finder probes (GFs). The system operates by exploiting the electrostatic interactions between positively charged CCPs and negatively charged DNA, facilitating sensitive and specific Dex detection. The label-free FRET aptasensor platform demonstrated robust performance in detecting Dex, exhibiting high selectivity and sensitivity. The system effectively distinguished Dex from interfering molecules and achieved stable detection across a range of concentrations in a commonly used sports drink matrix. Overall, the label-free FRET Dex detection system offers a simple, cost-effective, and highly sensitive approach for detecting Dex in diverse sample matrices. Its simplicity and effectiveness make it a promising tool for anti-doping efforts and other applications requiring rapid and accurate Dex detection.

As an inflammatory disease with a disrupted immune system, cytokine disorders in atopic dermatitis (AD) are closely related to the abnormal activation of JAK-STAT signal pathway. The critical relevance of the JAK-STAT signaling pathway to the pathogenesis of AD provides a strong rationale for JAK inhibitor research. Baricitinib, a small-molecule oral JAK inhibitor, has been proven to inhibit JAK-STAT signaling in a variety of diseases, including AD. It is currently available in China for off-label use. However, its efficacy in China and its mechanism are rarely reported. In our study, we found that the immune status of patients with moderate and severe AD was hyperactive. Among the 49 known immunotherapy targets, JAK1 and JAK2 genes on lymphocytes of AD patients were significantly upregulated, which was closely related to the symptom severity in moderate and severe AD patients. Baricitinib can improve immune hyperresponsiveness and clinical symptoms in moderate and severe AD by inhibiting the activation of Th2 cell subsets and the secretion of Th2-type cytokines through MAPK, mTOR and PI3K-Akt signaling pathways, providing an important theoretical basis for clinical off-label use of Baricitinib to treat moderate and severe AD.

Although significant progress has been made in developing autonomous vehicle technology, further research is still needed to reduce the risk of collision by planning a suitable path. Drawing vehicle paths through pedestrian collision avoidance action is worth further study. In this paper, vehicle path planning considering pedestrian collision avoidance action is proposed to improve vehicle driving safety further. First, the movement features reflecting different types of pedestrian collision avoidance intention are summarized through the actual human-vehicle collision accident video. Then, the data representing the pedestrian action features are obtained through the deep neural network and input into the trained Hidden Markov Model to predict pedestrian intention. Finally, the vehicle collision avoidance path is planned through the cubic polynomial combined with the pedestrian intention. The algorithm is verified through the pedestrian collision avoidance action in the simulation scene. The results show that this algorithm can predict the intention of different types of pedestrians to avoid collisions at frames 9, 8, and 10, respectively, which is 0.6 seconds, 0.4 seconds, and 0.6 seconds faster than the Long Short Term Memory algorithm. Finally, a suitable path is planned, and the collision was successfully avoided.

Abstract Cross-domain few-shot learning is one of the research highlights in machine learning. The difficulty lies in the accuracy drop of cross-domain network learning on a single domain due to the differences between the domains. To alleviate the problem, according to the idea of contour cognition and the process of human recognition, we propose a few-shot learning method based on pseudo-Siamese convolution neural network. The original image and the sketch map are respectively sent to the branch network in the pre-training and meta-learning process. While maintaining the original image features, the contour features are separately extracted as branch for training at the same time to improve the accuracy and generalization of learning. We conduct cross-domain few-shot learning experiments and good results have been achieved using mini-ImageNet as source domain, EuroSAT and ChestX as the target domains. Also, the results are qualitatively analyzed using a heatmap to verify the feasibility of our method.

Abstract Rural revitalization strategies are an important task in China. Currently, it is in the transition from poverty alleviation to rural revitalization. This paper proposes an evaluation index of rural revitalization and development potential based on a summary of previous studies. Together with the TOPSIS method, the corresponding coefficients of each index layer and the weight coefficient of the criterion layer were analyzed. This shows that during the process, the work direction of rural revitalization varies based on different revitalization types. In this study, diagnostic tools are utilized to conduct a potential development analysis of rural human settlements by identifying the main influencing factors for rural revitalization. In addition, an index system for improving rural human settlement strategies is established. Overall, it helps in defining the interventions of reducing and managing the risk of rural vitalization and evaluating the potential ability of rural revitalization. It also suggests that Anhui Province should focus on carrying out the comprehensive revitalization of rural areas according to the different functional positions of the countryside.

Cardiovascular disease remains one of the leading causes of death in China, with increasingly serious negative effects on people and society. Despite significant advances in preventing and treating cardiovascular diseases, such as atrial fibrillation/flutter and heart failure over the last few years, much more remains to be done. Therefore, developing innovative methods for identifying and managing cardiovascular disorders is critical. Nanomaterials provide multiple benefits in biomedicine, primarily better catalytic activity, drug loading, targeting, and imaging. Biomimetic materials and nanoparticles are specially combined to synthesize biomimetic nanoparticles that successfully reduce the nanoparticles’ toxicity and immunogenicity while enhancing histocompatibility. Additionally, the biological targeting capability of nanoparticles facilitates the diagnosis and therapy of cardiovascular disease. Nowadays, nanomedicine still faces numerous challenges, which necessitates creating nanoparticles that are highly selective, toxic-free, and better clinically applicable. This study reviews the scientific accomplishments in this field over the past few years covering the classification, applications, and prospects of noble metal biomimetic nanozymes and biomimetic nanocarriers.

This paper explores advanced shape control techniques for ultra-lightweight electro-actuated polymers with composite ferroelectric thin films. It begins with an overview of PVDF-TrFE film actuators used in the development of thin-shell composites, emphasizing the need to overcome constraints related to the electrode size for successful scalability. Strain generation in thin-film actuators is investigated, including conventional electrode-based methods and non-contact electron flux excitation. Numerical studies incorporate experimentally calibrated ferroelectric parameters, modeling non-contact actuation with an equivalent circuit representation. The potential distribution generated by electron flux injection highlights its potential for reducing print-through actuation issues. Additionally, the paper outlines a vision for the future of large thin-shell reflectors by integrating the discussed methods for charging ferroelectric polymer films. A hierarchical control strategy is proposed, combining macro- and micro-scale techniques to rectify shape errors in lightweight reflectors. These strategies offer the potential to enhance precision and performance in future spaceborne observation systems, benefiting space exploration and communication technologies.

This paper addresses the challenges of ground-based observation of Low Earth Orbit (LEO) targets using on-Earth telescopes and proposes solutions for improving image quality and tracking fast-moving objects under atmospheric turbulence conditions. The study investigates major challenges, including atmospheric turbulence-induced aberrations, blurring, telescope platform vibrations, and spatial variations caused by the target motion. A scenario simulation is conducted considering practical limitations, such as feasible time slots for monitoring and the number of available frames within the Field of View (FoV). The paper proposes a novel method for detecting LEO targets using Harris corner features and matching adjacent images upon the corrected frames by Adaptive Optics (AO), with a 38% reduction in the Mean Squared Error (MSE) achieved for certain frames within the isoplanatic angle. Additionally, a refinement strategy for deblurring images is proposed, combining the post-processing with Singular Value Decomposition (SVD) filtering; with a proper filtering factor (β=0.1), an almost complete collection of 14 corner nodes can be detected. The feasibility of continuously tracking objects with uncontrolled attitudes, such as space debris, is successfully demonstrated with continuous monitoring of certain features. The proposed methods offer promising solutions for ground-based observation of LEO targets, providing insights for further research and application in this field.

Abstract With exquisite design and unique style, the painted sculptures of Tutang Buddha and two attendants Buddha in Jingyin Temple are precious cultural heritages of China. The sculpture of Tutang Buddha, which was carved from a mound and painted by ancient craftsmen, was rarely found in ancient China. However, due to natural and human factors, the sculptures were severely damaged. Obviously, they require urgent and appropriate protection and restoration. In this study, samples taken from the sculptures were analysed through multiple analytical techniques, including scanning electron microscopy with energy dispersive spectrometry (SEM–EDS), Raman spectroscopy, X-ray diffraction (XRD), optical microscopy (OM) and granulometry. The analysis results enable us to infer the techniques used by the craftsmen in making the sculptures and provide a reliable evidence for the conservation and future protection of these and similar sculptures.

Abstract The restoration of incomplete artifacts is important for presenting the value of artifacts. Even though the existing methods provide rich solutions for the restoration of artifacts, they may not be the ultimate and perfect methods. The innovation of restoration methods may provide better restoration effects in some aspects or bring people a different visiting experience, which motivates people to explore new restoration methods. In this paper, based on virtual imaging technology, a virtual imaging restoration method for incomplete artifacts were proposed. In order to examine the effects and potential of the virtual imaging restoration, a bronze mirror and a mural were restored through our newly invented virtual imaging restoration device. The effects of the restorations were evaluated through the illumination test and the questionnaire surveys. The results proved that the virtual imaging restoration can visually achieve in situ restoration, and it is completely reversible. Virtual imaging restoration also performed well in terms of visiting experience, authenticity, discernibility, and economy. Limitations of this restoration method are related to the scope of application, the viewing angle, and the imaging effects. Overall, virtual imaging restoration is worth recommending and the potential of this method and its encouraging results may provide a choice or pretest tool for the restoration of some incomplete artifacts in the near future.

Abstract The precious collections of the Qing Dynasty paper-made theatrical figurines known as Shage xiren (纱阁戏人) represent a unique type of Chinese traditional folk art. The name, “Shage xiren” can be explained as the theatrical figurines that display in gauze curtains covered showcases. There were originally 36 showcases of Shage xiren of which 34 are preserved with 28 showcases exhibited at Qingxu Taoist Temple of Pingyao and the other damaged 6 are kept in the storeroom. It is rare that ancient paper-made figurines can be retained; therefore Shage xiren became a valuable source for understanding the traditional folk art as well as exploring rarely inherited crafts. Due to the material specificity and the poor preservation conditions, most of the figurines are found with various damages. It appears necessary to carry out appropriate conservative and restorative actions. To date, very few works have been reported on investigating the materials and crafts of making Shage xiren, resulting in a lack of basis for restoration. In this research, the samples collected from the damaged figurines, including wooden stick, plant fibers, paper pieces, clay fragments and textile fibers were studied. Polarized light microscope, X-ray diffraction, granulometry, Raman spectrum, digital microscopy and Fourier transform infrared spectrum analyses were employed to characterize the materials used in producing the cultural relics. The results indicated that not only paper, but also various materials were utilized in making the figurines. To form well-shaped bodies, the figurines have moso bamboo sticks applied as supporting structures, then covered with wheat straw and filled with natural cotton. The paper costumes and decorations were made from coniferous wood pulp and bamboo fibers respectively, and the textile belts were made from silk. The heads, feet and fingers of the figurines were shaped with fine local clay mixed with natural fibers to prevent cracking. The heads were further applied with lime to improve the surface smoothness and brightness. The pigments of red lead, basic lead carbonate, indigo and artificial ultramarine were used in painting and dyeing the clay heads and feet, and paper decorations. Although the figurines were made from accessible materials, the ingenious design and the sophisticated skills combining sculpturing, paper folding and elaborate ornamenting made it one of the unique artefacts. This research further provided reliable evidence in guiding the conservation and restoration work.

As an emerging 3D printing technology, 3D bioprinting has shown great potential in tissue engineering and regenerative medicine. Decellularized extracellular matrices (dECM) have recently made significant research strides and have been used to create unique tissue-specific bioink that can mimic biomimetic microenvironments. Combining dECMs with 3D bioprinting may provide a new strategy to prepare biomimetic hydrogels for bioinks and hold the potential to construct tissue analogs in vitro, similar to native tissues. Currently, the dECM has been proven to be one of the fastest growing bioactive printing materials and plays an essential role in cell-based 3D bioprinting. This review introduces the methods of preparing and identifying dECMs and the characteristic requirements of bioink for use in 3D bioprinting. The most recent advances in dECM-derived bioactive printing materials are then thoroughly reviewed by examining their application in the bioprinting of different tissues, such as bone, cartilage, muscle, the heart, the nervous system, and other tissues. Finally, the potential of bioactive printing materials generated from dECM is discussed.

This paper provides a multi-agent coordinated control system to improve the real-time performance of intelligent vehicle active collision avoidance. At first, the functions and characteristics of longitudinal and lateral collision avoidance agents are analyzed, which are the main components of the multi-agent. Then, a coordinated solution mechanism of an intelligent vehicle collision avoidance system is established based on hierarchical control and blackboard model methods to provide a reasonable way to avoid collision in complex situations. The multi-agent coordinated control system can handle the conflict between the decisions of different agents according to the rules. Comparing with existing control strategies, the proposed system can realize multi decisions and planning at the same time; thus, it will reduce the operation time lag during active collision avoidance. Additionally, fuzzy sliding mode control theory is introduced to guarantee accurate path tracking in lateral collision avoidance. Finally, co-simulation of Carsim and Simulink are taken, and the results show that the real-time behavior of intelligent vehicle collision avoidance can be improved by 25% through the system proposed.

Abstract Over the years, numerous gold and silver artifacts were excavated from tombs of the Tang Dynasty which gave evidence of the sophisticated metalworking techniques at that time. However, very few of them were studied and the manufacturing process was barely known. In this research, the microstructural analyses including microscopy and SEM–EDS were conducted on a delicate silver cosmetics box excavated from a Tang Dynasty tomb of Xiaolizhuang site. The silver artifact is made up of two symmetrical cambered pieces which were fabricated by alternating hammering and annealing. The shape and surface features were further modified through plastic forming. Afterwards, spinning technique was employed to ensure the interior surface is smooth and has consistent thickness. The surface of the silver box is fully decorated with multiple fine engraving including petals, fish-toe circles, flower pattern, twine pattern and the Tang grass pattern. According to the overlapping status of the notches, the patterns were processed in a certain order by using various gravers. The artifact was brought in its final appearance by gilding to form a thin gold film on the surface. These results overall reveal the sophisticated techniques applied in making the artifact and further enhance the understanding of metalworking practices in the Tang Dynasty.

Two-dimensional solids are of interest for energy storage due to their large accessible surface area, enabling rapid charge/discharge. Here, the authors quantify the point defects in oxide nanosheets, demonstrating that intentional introduction of charged point defects improves the charge storage behaviour.

CuAlO2 has been examined as a potential luminescent material by substituting Eu for Al cations in the delafossite structure. CuAlO2:Eu3+ nanofibers have been prepared via electrospinning for the ease of mitigating synthesis requirements and for future optoelectronics and emerging applications. Single-phase CuAlO2 fibers could be obtained at a temperature of 1100 °C in air. The Eu was successfully doped in the delafossite structure and two strong emission bands at ~405 and 610 nm were observed in the photoluminescence spectra. These bands are due to the intrinsic near-band-edge transition of CuAlO2 and the f-f transition of the Eu3+ activator, respectively. Further electrical characterization indicated that these fibers exhibit semiconducting behavior and the introduction of Eu could act as band-edge modifiers, thus changing the thermal activation energies. In light of this study, CuAlO2:Eu3+ fibers with both strong photoluminescence and p-type conductivity could be produced by tailoring the rare earth doping concentrations.

Pottery, bone implements, and stone tools are routinely found at Neolithic sites. However, the integrity of textiles or silk is susceptible to degradation, and it is therefore very difficult for such materials to be preserved for 8,000 years. Although previous studies have provided important evidence of the emergence of weaving skills and tools, such as figuline spinning wheels and osseous lamellas with traces of filament winding, there is a lack of direct evidence proving the existence of silk. In this paper, we explored evidence of prehistoric silk fibroin through the analysis of soil samples collected from three tombs at the Neolithic site of Jiahu. Mass spectrometry was employed and integrated with proteomics to characterize the key peptides of silk fibroin. The direct biomolecular evidence reported here showed the existence of prehistoric silk fibroin, which was found in 8,500-year-old tombs. Rough weaving tools and bone needles were also excavated, indicating the possibility that the Jiahu residents may possess the basic weaving and sewing skills in making textile. This finding may advance the study of the history of silk, and the civilization of the Neolithic Age.

Archaeological silk provides abundant information for studying ancient technologies and cultures. However, due to the spontaneous degradation and the damages from burial conditions, most ancient silk fibers which suffered the damages for thousands of years were turned into invisible molecular residues. For the obtained rare samples, extra care needs to be taken to accurately identify the genuine archaeological silk remains from modern contaminations. Although mass spectrometry (MS) is a powerful tool for identifying and analyzing the ancient protein residues, the traditional approach could not directly determine the dating and contamination of each sample. In this paper, a series of samples with a broad range of ages were tested by MS to find an effective and innovative approach to determine whether modern contamination exists, in order to verify the authenticity and reliability of the ancient samples. The new findings highlighted that the detected peptide types of the fibroin light chain can indicate the degradation levels of silk samples and help to distinguish contamination from ancient silk remains.

As an inflammatory disease with a disrupted immune system, cytokine disorders in atopic dermatitis (AD) are closely related to the abnormal activation of JAKSTAT signal pathway. The critical relevance of the JAK-STAT signaling pathway to the pathogenesis of AD provides a strong rationale for JAK inhibitor research. Baricitinib, a small-molecule oral JAK inhibitor, has been proven to inhibit JAKSTAT signaling in a variety of diseases, including AD. It is currently available in China for off-label use. However, its efficacy in China and its mechanism are rarely reported. In our study, we found that the immune status of patients with moderate and severe AD was hyperactive. Among the 49 known immunotherapy targets, JAK1 and JAK2 genes on lymphocytes of AD patients were significantly upregulated, which was closely related to the symptom severity in moderate and severe AD patients. Baricitinib can improve immune hyperresponsiveness and clinical symptoms in moderate and severe AD by inhibiting the activation of Th2 cell subsets and the secretion of Th2-type cytokines through MAPK, mTOR and PI3K-Akt signaling pathways, providing an important theoretical basis for clinical off-label use of Baricitinib to treat moderate and severe AD. [ABSTRACT FROM AUTHOR]

Thin films, thinner than the diameter of a human hair (~90µm and below), hold considerable promise across various applications such as flexible electronics, thin film devices, and biomedical applications due to their distinct characteristics. At this juncture, the mechanical properties of these films play a pivotal role as they underpin the stability of these applications. However, evaluating the mechanical properties of these thin films poses a significant technical challenge owing to their minimal thickness, where conventional technologies often prove inadequate. Over the past decade, notable advancements have been made in characterization techniques to surmount this challenge, particularly for ultra-thin films. This review focuses on recently developed experimental techniques used for characterizing the mechanical properties of thin films thinner than the diameter of a human hair, but beyond two-dimensional materials. We introduce these experimental testing techniques, analyze their pros and cons, and discuss their main applications. The mechanical properties of ultrathin films beyond 2D materials discussed in this study include elastic modulus, hardness, in-plane strength, and fracture toughness. We conclude this review article with our perspective on the applications and future research directions of thin films in the current state of the field. [ABSTRACT FROM AUTHOR]