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Persulfate (PS)-based advanced oxidation processes have drawn tremendous attention for the degradation of recalcitrant pollutants, and cobalt composites are effective for PS activation to generate reactive species. In this study, composites of cobalt species loaded on cherry core-derived biochar (Co/C) were prepared with a one-step pyrolysis method. The Co/C catalyst was applied as a catalyst for PS activation to degrade bisphenol A (BPA). Factors influencing the degradation efficiency were examined, including the ratio of raw materials, Co/C and PS dosages, temperature, and solution pH. The Co/C catalyst prepared when the ratio of raw material was 1 : 1 (Co/C-50) could efficiently activate both peroxymonosulfate (PMS) and peroxydisulfate (PDS). When the initial concentration of BPA was 20 mg L

A cell membrane barrier which dominates the therapeutic efficacy and systemic side effects is a major bottleneck in the field of drug delivery. Herein, a therapeutic system capable of photothermally triggered on-demand and cytosolic delivery was achieved by polydopamine (PDA) nanoparticle-stabilized colloidosomes. An organic phase change material (PCM, saturated fatty acids) was employed as the lipid core for Pickering emulsification and drug encapsulation, and arginine was utilized as a linker to induce the directional interactions between nanoemulsion droplets and heterogeneously nucleated PDA nanoparticles. Moreover, the PDA particle stabilizers concomitantly mediated the grafting of hydrophilic polymer PEG to further improve dispersibility. The resultant colloidosomes after cooling possess lowered melting points and superior dispersion stability over 7 days. When irradiated with near-infrared light (808 nm), sequential processes of fatty acid melting and direct drug perfusion into the cytosol took place within 10 min. The employment of vorinostat (SAHA, histone deacetylase inhibitor) as a model membrane-impermeable drug resulted in remarkable enhancement of anti-cancer effects both

A 4D dual-mode staring hyperspectral-depth imager (DSHI), which acquire reflectance spectra, fluorescence spectra, and 3D structural information by combining a staring hyperspectral scanner and a binocular line laser stereo vision system, is introduced. A 405 nm laser line generated by a focal laser line generation module is used for both fluorescence excitation and binocular stereo matching of the irradiated line region. Under the configuration, the two kinds of hyperspectral data collected by the hyperspectral scanner can be merged into the corresponding points in the 3D model, forming a dual-mode 4D model. The DSHI shows excellent performance with spectral resolution of 3 nm, depth accuracy of 26.2 µm. Sample experiments on a fluorescent figurine, real and plastic sunflowers and a clam are presented to demonstrate system’s with potential within a broad range of applications such as, e.g., digital documentation, plant phenotyping, and biological analysis.

A novel tactile sensor for two-dimensional force location measurements, based on polymer-based planar waveguide chirped Bragg gratings (PPCBGs) fabricated on sheet PMMA substrate, is presented. The planar waveguide and chirped Bragg grating are simultaneously generated using a KrF excimer laser and a phase mask covered by a quartz chrome mask. Location and magnitude of an applied force is measured by observing the change of the wavelength of a dip in the measured spectrum and a change in the reflectivity intensity. Experimental characterization indicates submillimeter spatial resolution of applied force in the range of 1-4 N with a sensitivity of 947.02 pm/mm.

Nanoquencher-based biosensors have emerged as powerful tools for the detection of tumor markers, where challenges in efficiently docking the π-electron interaction interface toward nucleic acid probes containing π-electron-rich units of bases and fluorescent dyes still remain. Herein, we present hybrid polydopamine/polypyrrole nanosheets (PDA-PPy-NS) with π electron coupling and ultranarrow band gap (0.29 eV) by interfacial engineering of polymer hybrids at the nanoscale. PDA-PPy-NS were first prepared through oxidant-induced polymerization of pyrrole on PDA nanosheets. By utilizing fluorescent-dye-labeled single-stranded DNA as a probe, the hybrid nanoquencher showed ultrahigh fluorescence quenching ability, i.e., a Cy5-ssDNA/nanoquencher mass ratio of 36.9 under the complete quenching condition, which is comparable to that of graphene oxide. It was demonstrated that the energy level coupling of nanosheets and nucleic acid dye (Cy5) was the key factor contributing to the efficient photoinduced electron transfer (PET). Subsequently, the nanoquencher/DNA probe was proved to possess superior sensitivity and selectivity for efficient and reliable detection of miRNA-21 with a detection limit of 23.1 pM. Our work proves that the π-electron-rich biosensor interface can significantly enhance the PET efficiency, providing a theoretical basis for developing novel high-performance sensors.

Heterogeneous nuclear ribonucleoproteins (hnRNPs) are RNA-binding proteins that are reported to play a crucial role in the pathogenic process of multiple malignancies. However, their expression patterns, clinical application significance and prognostic values in invasive breast carcinoma (BRCA) remain unknown. In this study, we investigated hnRNP family members in BRCA using accumulated data from Oncomine 4.5, UALCAN Web portal and other available databases. We explored the expression and prognostic value level of hnRNPs in BRCA. We further analyzed their association with the clinicopathological features of BRCA patients. Subsequently, we calculated the alteration frequency of hnRNPs, constructed the interaction network of hnRNPs, and examined the potential coexpression genes of hnRNPs, revealing that HNRNPU and SYNCRIP are the core molecular genes requiring further investigation for BRCA. We validated the immunohistochemistry (IHC) pattern to simulate clinical applications based on pathology. Cell function experiments conducted in vitro indicated that HNRNPU can promote epithelial-mesenchymal transition, functionally stimulating the invasion capacity and inhibiting the viability of invasive BRCA cells. In summary, our systematic analysis demonstrated that HNRNPU was the key molecule that played a fundamental role in BRCA metastasis, which may facilitate the development of new diagnostic and prognostic markers for the analysis of BRCA progression.

Polydopamine (PDA) is an artificial melanin polymer that has been spotlighted due to its extraordinary optoelectronic characteristics and advance theranosctic applications in biomaterial fields. Moreover, interactions on the nano-bio interface interplay whereby substances exchange in response to endogenous or exogenous stimuli, and electron transfer driven by light, energy-level transitions, or electric field greatly affect the functional performance of PDA-modified nanoparticles. The full utilization of potential in PDA's interfacial activities, optoelectrical properties and related responsiveness is therefore an attractive means to construct advanced nanostructures for regulating biological processes and metabolic pathways. Herein, we strive to summarize recent advances in the construction of functional PDA-based nanomaterials with state-of-the-art architectures prepared for modulation of photoelectric sensing and redox reversibility, as well as manipulation of photo-activated therapeutics. Meanwhile, contributions of interfacial electron transfer and matter conversion are highlighted by discussing the structure-property-function relationships and the biological effects in their featured applications including disease theranostics, antibacterial activities, tissue repair, and combined therapy. Finally, the current challenges and future perspectives in this emerging research field will also be outlined. Recent advances on polydopamine-based nanotherapeutics with an emphasis on their interfacial activities, optoelectrical properties and related responsiveness are reviewed for providing insightful guidance to the rational design of integrated theranostic nanoplatforms with high performance in the biomedical fields. This article is categorized under: Diagnostic ToolsDiagnostic Nanodevices Therapeutic Approaches and Drug DiscoveryNanomedicine for Oncologic Disease Nanotechnology Approaches to BiologyNanoscale Systems in Biology.

Fluorescent nanosensor-based tumor imaging holds great promise in cancer diagnosis and treatment assistance, yet the signal contrast is heavily hampered by the unspecific/unwanted activation at microscopic regions with a highly restricted local abundance of biomarkers. Herein, we developed an activation boosting strategy by the integration and manipulation of dual-factor coactivation of sensing and lysosome escape facilitated the rise of cytosolic biomarker accessibility. By employing hybrid DNA probes on gold nanoquenchers, ATP sensing initiated conformation switch of the corresponding aptamer units triggered the exposure of a hidden toehold in a loop structure. Sequentially, miRNA-21 sensing was triggered by toehold-mediated strand displacement and detachment of the binding complexes. The application of lysosomotropic agent chloroquine at optimized time interval facilitated the release of nanosensors into the cytosol and a ∼10.5-fold increment of intracellular fluorescence in vitro, while coactivation improved the cancer-to-normal cell signal ratio by ∼5.9 times. The synergy effects led to a high tumor-to-normal tissue ratio value of ∼7.9 in the in vivo imaging results. This strategy establishes a new paradigm of fluorescent nanosensors for selective and specific tumor imaging.

Funder: National Physical Laboratory; doi: http://dx.doi.org/10.13039/501100007851, Funder: Royal Society Dorothy Hodgkin Research Fellowship, The gas sensor market is growing fast, driven by many socioeconomic and industrial factors. Mid-infrared (MIR) gas sensors offer excellent performance for an increasing number of sensing applications in healthcare, smart homes, and the automotive sector. Having access to low-cost, miniaturized, energy efficient light sources is of critical importance for the monolithic integration of MIR sensors. Here, we present an on-chip broadband thermal MIR source fabricated by combining a complementary metal oxide semiconductor (CMOS) micro-hotplate with a dielectric-encapsulated carbon nanotube (CNT) blackbody layer. The micro-hotplate was used during fabrication as a micro-reactor to facilitate high temperature (>700 ∘C) growth of the CNT layer and also for post-growth thermal annealing. We demonstrate, for the first time, stable extended operation in air of devices with a dielectric-encapsulated CNT layer at heater temperatures above 600 ∘C. The demonstrated devices exhibit almost unitary emissivity across the entire MIR spectrum, offering an ideal solution for low-cost, highly-integrated MIR spectroscopy for the Internet of Things.

Electrochemical nanosensors by integrating functional nucleic acids and nanomaterials hold a great promise in the fast detection of biomarkers, yet the current systems possess limitations on the accessibility of target-probe and probe-electrode interactions and the repeatability of detection. Herein, a host-guest assembly strategy is developed to build redox nanosensors for an immobilization-free and ratiometric electrochemical detection system. Specifically, electroactive molecule (E

Metal-free polymer daytime radiative cooling coatings with hierarchical eye-like air pores are proposed and fabricated with a super-large-scale film-stretching method. The hierarchically porous film (HPF) can be further coated with polymethyl methacrylate (PMMA) micro-hemispheres, forming coated HPF (cHPF), which do not dramatically change the optical or thermal properties. The cHPF is slightly better with a lower solar absorptivity (2.4%) and a higher thermal emissivity over the atmospheric transparency window (90.1%). The low solar absorptivity is due to the strong scattering of the hierarchical eye-like air pores, while the molecular vibrations and the focusing effect of the PMMA micro-hemispheres contribute to the high emissivity. An average mid-day temperature reduction of 7.92 °C is achieved relative to the air temperature, and the average cooling power reaches 116.0 W m

An optical system for gaseous chloroform (CHCl3) detection based on wavelength modulation photoacoustic spectroscopy (WMPAS) is proposed for the first time by using a distributed feedback (DFB) laser with a center wavelength of 1683 nm where chloroform has strong and complex absorption peaks. The WMPAS sensor developed possesses the advantages of having a simple structure, high-sensitivity, and direct measurement. A resonant cavity made of stainless steel with a resonant frequency of 6390 Hz was utilized, and eight microphones were located at the middle of the resonator at uniform intervals to collect the sound signal. All of the devices were integrated into an instrument box for practical applications. The performance of the WMPAS sensor was experimentally demonstrated with the measurement of different concentrations of chloroform from 63 to 625 ppm. A linear coefficient R2 of 0.999 and a detection sensitivity of 0.28 ppm with a time period of 20 s were achieved at room temperature (around 20 °C) and atmosphere pressure. Long-time continuous monitoring for a fixed concentration of chloroform gas was carried out to demonstrate the excellent stability of the system. The performance of the system shows great practical value for the detection of chloroform gas in industrial applications.

In this work we present a novel multi-sensor unit, a.k.a. FireNose, to detect and discriminate both known and unknown gases in uncontrolled conditions to aid firefighters under harsh conditions. The unit includes three metal oxide (MOX) gas sensors with CMOS micro heaters, a plasmonic enhanced non-dispersive infrared (NDIR) sensor optimized for the detection of CO2, a commercial temperature humidity sensor, and a flow sensor. We developed custom film coatings for the MOX sensors (SnO2, WO3 and NiO) which greatly improved the gas sensitivity, response time and lifetime of the miniature devices. Our proposed system exhibits promising performance for gas sensing in harsh environments, in terms of power consumption (~ 35 mW at 350°C per MOX sensor), response time (< 10 s), robustness and physical size. The sensing unit was evaluated with plumes of gases in both, a laboratory setup on a gas testing rig and on-board a mobile robot operating indoors. These high sensitivity, high-bandwidth sensors, together with online unsupervised gas discrimination algorithms, are able to detect and generate their spatial distribution maps accordingly. In the robotic experiments, the resulting gas distribution maps corresponded well to the actual location of the sources. Therefore, we verified its ability to differentiate gases and generate gas maps in real-world experiments.

Currently, the simple integration of multiple therapeutic agents within a single nanostructure for combating multidrug resistance (MDR) tumors yet remains a challenge. Herein, we report a photoresponsive nanocluster (NC) system prepared by installing polydopamine (PDA) nanoparticle clusters on the surface of d-α-tocopheryl poly(ethylene glycol) 1000 succinate (TPGS) (a drug efflux inhibitor) micelles solubilized with IR780 (a photosensitizer) to achieve a combined chemotherapy (CT)/photothermal therapy (PTT)/photodynamic therapy (PDT) for drug-resistant breast cancer. Mediated by the fluorescence resonance energy transfer and radical scavenging properties of PDA, NC shows prominently quenched fluorescence emission (∼78%) and inhibited singlet oxygen generation (∼67%) upon exposure to near-infrared (NIR) light (808 nm, 0.5 W cm-2), favoring a highly efficient PTT module. Meanwhile, the photothermal heat can also boost the release of doxorubicin hydrochloride whose intracellular accumulation can be greatly enhanced by TPGS. Interestingly, the first NIR irradiation and subsequent incubation (∼24 h) can induce the gradual relocation and disintegration of PDA nanoparticles, thereby leading to activated PDT therapy under the second irradiation. Upon the temporally controlled sequential application of PTT/PDT, the developed NC exhibited a great potential to treat MDR cancer both in vitro and in vivo. These findings suggest that complementary interactions among PTT/PDT/CT modalities can enhance the efficiency of the combined therapy for MDR tumor.

The translation of mussel-inspired wet adhesion to biomedical engineering fields have catalyzed the emergence of polydopamine (PDA)-based nanomaterials with privileged features and properties of conducting multiple interfacial interactions. Recent concerns and progress on the understanding of PDA's hierarchical structure and progressive assembly are inspiring approaches toward novel nanostructures with property and function advantages over simple nanoparticle architectures. Major breakthroughs in this field demonstrated the essential role of π-π stacking and π-cation interactions in the rational intervention of PDA self-assembly. In this review, the recently emerging concepts in the preparation and application of PDA nanomaterials, including 3D mesostructures, low-dimensional nanostructures, micelle/nanoemulsion based nanoclusters, as well as other multicomponent nanohybrids by the segregation and organization of PDA building blocks on nanoscale interfaces are outlined. The contribution of π-electron interactions on the interfacial loading/release of π electron-rich molecules (nucleic acids, drugs, photosensitizers) and the exogenous coupling of optical energy, as well as the impact of wet-adhesion interactions on the nano-bio interface interplay, are highlighted by discussing the structure-property relationships in their featured applications including fluorescent biosensing, gene therapy, drug delivery, phototherapy, combined therapy, etc. The limitations of current explorations, and future research directions are also discussed.

At present, the traditional curriculum system of the hotel management major takes professional technology and skill courses as the core courses to cultivate the basic knowledge and service skills of modern hotel operation and management, and possess the applied first-line professionals required by the modern hotel industry. However, the current research and practice of Innovative entrepreneurship education in higher vocational colleges are mostly concentrated in the respective fields of "entrepreneurship education" and "professional education", and there is a widespread phenomenon of alienation between Innovative entrepreneurship education and majors and disciplines. Zun follows the modern vocational education concept of "building morality, facing everyone, and lifelong education". According to the needs of the development of tourism and accommodation industry for compound and innovative talents and the needs of students' individual career development, the talent training objectives and curriculum system are dynamically adjusted. Therefore, it is necessary to innovate hotel management work in time, so as to make it occupy an important position in the increasingly competitive market environment. This paper mainly focuses on the reform of entrepreneurial talents training mode in higher vocational colleges. The goal of entrepreneurship education can be effectively realized only by the combination of learning and thinking, the unity of knowing and doing, and teaching students in accordance with their aptitude.

This paper presents the development of a low cost and low power wearable Electronic Nose with IoT connectivity for detecting synthetic urine. This system was designed with the ultimate aim to relieve the burdens associated with urinary incontinence for both sufferers and healthcare workers in hospitals and care homes. Commercially proven metal oxide (MOX) gas sensors for Volatile Organic Compound (VOC) detection such as the SGP30 and MiCS-5524 series of gas sensors were used, as well as the custom-made WO3, SnO2 and CuO MOX sensors developed at the Microsensors and Bioelectronics laboratory at the University of Warwick. A web-application was created to facilitate real-time monitoring over the MQTT protocol and indicate when urine has been detected. The capabilities of the IoT for this purpose allow for enhanced patient mobility and simultaneous patient monitoring. An artificial neural network was used to classify synthetic urine from other odours. The model was able to identify synthetic urine with 100% accuracy based on prior training and testing datasets.

OBJECTIVE: To investigate serum levels of long non-coding RNA (lncRNA) TUSC7 in patients with esophageal squamous cell carcinoma (ESCC), its association with clinicopathological parameters and its role in promoting tumor metastasis and invasion. METHODS: Serum samples were collected from 60 patients with ESCC admitted between January, 2017 and May, 2019, with 60 age- and gender-matched healthy subjects as the control group. Serum level of TUSC7 in ESCC patients and its expression in 4 ESCC cell lines was detected with RT-qPCR. The association of serum TUSC7 level with the clinicopathological features of the patients was analyzed. KYSE-30 cell models with TUSC7 overexpression or knockdown were established, and the proliferation of the cells was examined with MTT assay and their migration and invasion were assessed using wound healing and Transwell assays. Western blotting was used to detect the cellular expressions of the proteins associated with epithelial-mesenchymal transition (EMT). RESULTS: The patients with ESCC had significantly lower serum TUSC7 level than the healthy control subjects (P < 0.05). The ESCC cell lines also expressed lower levels of TUSC7 than normal cells (P < 0.05). Serum TUSC7 level was negatively correlated with tumor staging, lymph node metastasis and infiltration (P < 0.05) but was not significantly correlated with other clinicopathological parameters in ESCC patients. In the in vitro cell experiment, overexpression of TUSC7 in KYSE-30 cells significantly inhibited cell migration and invasion (P < 0.05), enhanced the expression of the EMT marker protein E-cadherin and lowered the expressions of N-cadherin, Vimentin and MMP9 (P < 0.05); knocking down TUSC7 in the cells produced the opposite effects. CONCLUSION: The down-regulation of TUSC7 expression in the serum of ESCC patients and in ESCC cell lines is associated with the metastasis of ESCC and promotes tumor cell migration and invasion by promoting EMT, indicating the potential of serum TUSC7 level as a molecular marker for diagnosis, treatment and metastasis monitoring of ESCC.

Cerebral ischemia-reperfusion injury (CIRI) is an important pathophysiological process of ischemic stroke associated with various physiological and pathological processes, including autophagy and apoptosis. In this study, we examined the role and mechanism of long noncoding RNA CAMK2D-associated transcript 2 (C2dat2) in regulating CIRI in vivo and in vitro. C2dat2 up-regulation facilitated neuronal autophagy and apoptosis induced by CIRI. Mechanistically, C2dat2 acts as a competing endogenous RNA (ceRNA) to negatively regulate miR-30d-5p expression. More specifically, miR-30d-5p targeted the 3'-untranslated region of DNA damage-inducible transcript 4 (DDIT4) and silenced its target mRNA DDIT4. Additionally, C2dat2 binding with heat shock cognate 70/heat shock protein 90 blocked RNA-induced silencing complex assembly to abolish the miR-30d-5p targeting of DDIT4 and inhibited miR-30d-5p to silence its target mRNA DDIT4. Further analysis showed that C2dat2 knockdown conspicuously inhibited the up-regulation of DDIT4 and Beclin-1 levels and LC3B II/I ratio and the down-regulation of P62 and phosphorylated mammalian target of rapamycin (mTOR)/mTOR and phosphorylated-P70S6K/P70S6K ratio in Neuro-2a cells after oxygen-glucose deprivation/reoxygenation. This study first revealed that C2dat2/miR-30d-5p/DDIT4/mTOR forms a novel signaling pathway to facilitate autophagy and apoptosis induced by CIRI, contributing to the better understanding of the mechanisms of CIRI and enriching the ceRNA hypothesis in CIRI.

Monitoring particulate matter concentrations is of particular importance within the overall assessment of indoor and outdoor air quality and its impact on human health. Bulk acoustic wave (BAW) technology offers low cost, robust alternative to widely employed optical measurements. However, due to its reasonably new application within the area of particulate measurements, an in-depth characterisation of this technology to temperature and humidity variations that are inevitably present within the indoor and outdoor environment is necessary step in order to determine its suitability for possible commercialisation. This work presents the characterisation of the temperature and humidity dependence of solidly mounted resonators for particulate matter sensing. Both theoretical results, obtained through modelling and simulation, and experimental result, obtained within the laboratory conditions, are analysed and compared. A 1.5 GHz resonator with a zinc oxide thin film is modelled using a one-dimensional equivalent circuit model, and finite-element methods based on both two-dimensional model and the three-dimensional model. The simulation results show that the temperature dependence of the resonator is strongly dependent on the material properties and crystal structure of the zinc oxide film. Our models estimate the temperature coefficient of frequency to be -30 to -40 ppm/Centigrade. This theoretical temperature dependence was comparable to experimentally measured value of ca. -49 ppm/Centigrade. In addition to temperature characterisation of discrete devices, the resonator was combined with read-out circuitry, which was also simulated and tested experimentally. The temperature coefficient of frequency in this case was found to be much higher at -220 ppm/Centigrade demonstrating the necessity for temperature control or temperature compensation within the complete system in practical applications. The effect of humidity was also investigated. The experimental mean resonant frequency shift per percent increase in relative humidity of the ambient air was found to be -3.8 kHz/%RH, while the models showed negligible sensitivity to humidity variations. Finally, preliminary experiments were conducted within the controlled lab environment showing promising result for possible application of this technology in particulate matter monitoring. The resonant frequency shift of approximately 300 kHz was measured after mass loading the sensing area of the resonator with estimated 24 ng of standard Arizona dust particles.

Nanoscale colloidal capsules are promising drug delivery carriers currently while the demand for multiple-step syntheses and the difficulties in achieving high capsule stability are key obstacles that have greatly restricted their development. Herein, we report a polydopamine (PDA) nanoparticle stabilized nanocapsule as a drug delivery system based on the combination of nanoparticle formation and capsule assembly/stabilization in one pot. In this system, an arginine modified linoleic acid nanoemulsion was employed as the template for the in situ generation/assembly of interfacially active PDA nanoparticles, while directional interaction pairs of carboxylate-guanidine and amino-PDA linked by arginine are involved in the assembly process. The nanocapsules possess an average size of 100 nm, high stability in biological media, and efficient lipophilic transfer of the loaded lipophilic cargo. Notably, the high biocompatibility of the nanocapsules and the non-endocytotic delivery to the cytosol of cancer cells were demonstrated in vitro. Furthermore, the efficient delivery of paclitaxel, as well as paclitaxel/doxorubicin dual cargo, was realized, resulting in the high inhibition of cancer cells. Altogether, the PDA nanoparticle stabilized nanocapsules open new opportunities for the development of promising nanocapsule platforms for biomedical delivery.

Combination chemotherapy with a modulator and a chemotherapeutic drug has become one of the most promising strategies for the treatment of multidrug resistance (MDR) in cancer therapy. However, the development of nanocarriers with a high payload and sequential release of therapeutic agents poses a significant challenge. In this work, we report a type of hybrid nanocarriers prepared by polydopamine (PDA) mediated integration of the mesoporous MSN core and the microporous zeolite imidazolate frameworks-8 (ZIF-8) shell. The nanocarriers exploit storage capacities for drugs based on the high porosity and molecular sieving capabilities of ZIF-8 for sequential drug release. Particularly, large amounts of an anticancer drug (DOX, 607 μg mg