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The objective of this paper is to study the global DNA Methylation in monozygotic (MZ) twins with cerebral palsy. Two pairs of twins (a cerebral palsy children, a normal child) admitted to the First Affiliated Hospital of Zhengzhou University were selected as subjects. The phenol-chloroform method was used to extract DNA from venous blood and micro satellite DNA genotyping technique was used to identify the eggs of the twins. DNA methylation fragments were enriched by MBD affinity column chromatography, followed by Solexa sequencing and bioinformatics analysis. In this study, we found that there were different DNA hypermethylation regions between each pair of twins and normal children through global DNA methylation analysis technique by analyzing the blood cells of two pairs of monozygotic twins with cerebral palsy and normal infants. The results revealed the region of DNA methylation and the protein coding genes of promoter region of common methylation of cerebral palsy were both higher than normal children. These common promoter hypermethylation genes in cerebral palsy are involved in a variety of biological processes such as membrane protein transport, neuronal development, apoptosis, and metabolism. Moreover, DNA methylation plays an important role in gene expression. We hypothesized that the onset of cerebral palsy in twins is associated with hypermethylation of the promoter which inhibiting the expression of hypermethylation genes in children with cerebral palsy. The current research provided a basis for further study of the large sample of twins and sporadic cerebral palsy.

Neonatal lactose intolerance syndrome is a series of digestive system symptoms caused by the lack of lactase, and could not fully digest the lactose in breast milk or cow milk. Lactose is one of the disaccharides mainly existed in mammalian milk. Lactose content in breast milk is 7.2g/100ml, cow milk is 4.7g/100ml. Dairy products are the main energy sources for the newborn, and lactose provides 20% energy for infants. During the growth of the newborn, lactose not only play a significant role in energy supply, but also involve in the development of the brain growing. This study mainly studied the lactose development features, the reasons for lactose intolerance, and the measures to treat lactose deficiency.

2124 neonates were monitored from February 2013 to August 2014, among which 1119 were admitted from outpatient department (outpatient group), 782 were transferred from other departments (other department group), and 223 were from other hospitals (other hospital group). Through it we explore the distribution of multidrug resistant organism in neonates, which were admitted to the hospital through various ways, and therefore analyze the risk factors of nosocomial infection to avoid cross infection of multi drug resistant organism in neonatology department. The results showed that 105 strains of multi drug resistant bacteria were detected in the neonatal department. Among them, there were 57 strains from the outpatient group, 27 from the other department group, and 21 from the other hospital group. Neonates with the hospitalization time of more than 14 days and low birth weight infants (1500 g) were the high-risk groups of drug-resistant strains in nosocomial infection. So the infection in neonates from other departments or hospitals should be strengthen, especially the prevention and control in neonates with the hospitalization time than 14 days and low birth weight infants (1500 g) in order to reduce the occurrence of multiple drug-resistant strains cross infection.