120 results on '"Yusuke Kito"'
Search Results
2. Feasibility of newly designed rotatable sphincterotome for endoscopic sphincterotomy (with video)
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Yasuki Hori, Kazuki Hayashi, Itaru Naitoh, Fumihiro Okumura, Kaiki Anbe, Katsuyuki Miyabe, Atsuyuki Hirano, Hiroki Takada, Naruomi Jinno, Michihiro Yoshida, Akihisa Kato, Kenta Kachi, Hidenori Sahashi, Akihisa Adachi, Tadashi Toyohara, Kayoko Kuno, Yusuke Kito, and Hiromi Kataoka
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Pancreatobiliary (ERCP/PTCD) ,ERC topics ,Quality and logistical aspects ,Training ,Quality management ,Performance and complications ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Published
- 2024
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3. Comparison of 19-gauge conventional and Franseen needles for the diagnosis of lymphadenopathy and classification of malignant lymphoma using endoscopic ultrasound fine-needle aspiration
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Mitsuru Okuno, Keisuke Iwata, Tsuyoshi Mukai, Yusuke Kito, Takuji Tanaka, Naoki Watanabe, Senji Kasahara, Yuhei Iwasa, Akihiko Sugiyama, Youichi Nishigaki, Yuhei Shibata, Junichi Kitagawa, Takuji Iwashita, Eiichi Tomita, and Masahito Shimizu
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19-gauge ,endoscopic ultrasound-guided fine-needle aspiration ,franseen needle ,lymphadenopathy ,malignant lymphoma ,Internal medicine ,RC31-1245 ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Background/Aims Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) using a 19-gauge needle is an efficient sampling method for the diagnosis of lymphadenopathy. This study compared 19-gauge conventional and Franseen needles for the diagnosis of lymphadenopathy and classification of malignant lymphoma (ML). Methods Patient characteristics, number of needle passes, puncture route, sensitivity, specificity, and accuracy of cytology/histology for lymphadenopathy were analyzed in patients diagnosed with lymphadenopathy by EUS-FNA using conventional or Franseen needles. Results Between 2012 and 2022, 146 patients met the inclusion criteria (conventional [n=70] and Franseen [n=76]). The median number of needle passes was significantly lower in the conventional group than in the Franseen group (3 [1–6] vs. 4 [1–6], p=0.023). There were no significant differences in cytological/histological diagnoses between the two groups. For ML, the immunohistochemical evaluation rate, sensitivity of flow cytometry, and cytogenetic assessment were not significantly different in either group. Bleeding as adverse events (AEs) were observed in three patients in the Franseen group. Conclusions Both the 19-gauge conventional and Franseen needles showed high accuracy in lymphadenopathy and ML classification. Considering sufficient tissue collection and the avoidance of AEs, the use of 19-gauge conventional needles seems to be a good option for the diagnosis of lymphadenopathy.
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- 2024
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4. Discrepancy in the Location of Prostate Cancer Indicated on Biparametric Magnetic Resonance Imaging and Pathologically Diagnosed Using Surgical Specimens
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Masayuki Tomioka, Keita Nakane, Makoto Kawase, Koji Iinuma, Daiki Kato, Kota Kawase, Tomoki Taniguchi, Yuki Tobisawa, Fumiya Sugino, Tetsuro Kaga, Hiroki Kato, Masayuki Matsuo, Yusuke Kito, Chiemi Saigo, Natsuko Suzui, Takayasu Ito, Tatsuhiko Miyazaki, Tamotsu Takeuchi, and Takuya Koie
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prostate cancer ,biparametric magnetic resonance imaging ,prostate biopsy ,tumor location ,diagnostic discrepancy ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Accurate diagnosis of the localization of prostate cancer (PCa) on magnetic resonance imaging (MRI) remains a challenge. We aimed to assess discrepancy between the location of PCa pathologically diagnosed using surgical specimens and lesions indicated as possible PCa by the Prostate Imaging Reporting and Data System on MRI. The primary endpoint was the concordance rate between the site of probable clinically significant PCa (csPCa) identified using biparametric MRI (bpMRI) and location of PCa in the surgical specimen obtained using robot-assisted total prostatectomy. Among 85 lesions identified in 30 patients; 42 (49.4%) were identified as possible PCa on MRI. The 85 PCa lesions were divided into positive and negative groups based on the bpMRI results. None of the patients had missed csPCa. Although the diagnostic accuracy of bpMRI was relatively high for PCas located in the middle of the prostate (p = 0.029), it was relatively low for PCa located at the base of the prostate, all of which were csPCas. Although current modalities can accurately diagnose PCa, the possibility that PCa is present with multiple lesions in the prostate should be considered, even if MRI does not detect PCa.
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- 2024
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5. The novel technique of drainage stenting using a tapered sheath dilator in endoscopic ultrasound‐guided biliary drainage
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Akihisa Kato, Michihiro Yoshida, Yasuki Hori, Kenta Kachi, Hidenori Sahashi, Tadashi Toyohara, Akihisa Adachi, Kayoko Kuno, Yusuke Kito, and Hiromi Kataoka
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biliary drainage ,bile leakage ,Endosheather ,EUS‐BD ,tapered sheath dilator ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Abstract During endoscopic ultrasound‐guided biliary drainage (EUS‐BD), there is a risk for bile leakage until stent deployment, which can result in severe peritonitis, particularly when passing a drainage stent becomes challenging despite tract dilation. There is no established method or dedicated device to optimize EUS‐BD. Therefore, we have developed a novel stent deployment technique using the tapered sheath dilator. To address the safety and technical aspects of the EUS‐BD technique, we retrospectively analyzed 11 consecutive patients who underwent EUS‐BD using the tapered sheath dilator. The procedure involved the insertion of a guidewire, followed by mechanical dilation using the tapered sheath dilator. Subsequently, the inner catheter was removed and drainage stents (up to 6 Fr in diameter) were deployed through the outer sheath. We found a 100% technical success rate for tract dilation and stent deployment; moreover, all patients achieved clinical success. The median time for dilation was 40 s (range, 8–198), whereas the median time from dilation to stent deployment was 10 min (range, 6–19). Notably, no cases of bile leakage or peritonitis were observed. In conclusion, the use of the integrated device for tract dilation and stent delivery system might provide a safe and straightforward technique for drainage stenting during EUS‐BD.
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- 2024
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6. Potential of Anti-Leukotriene Drugs as New Therapeutic Agents for Inhibiting Cholangiocarcinoma Progression
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Yusuke Kito, Kenta Kachi, Michihiro Yoshida, Yasuki Hori, Akihisa Kato, Hidenori Sahashi, Tadashi Toyohara, Kayoko Kuno, Akihisa Adachi, Kenji Urakabe, and Hiromi Kataoka
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cholangiocarcinoma ,montelukast ,zileuton ,drug repositioning ,cysteinyl leukotriene receptor 1 ,leukotriene D4 ,Organic chemistry ,QD241-441 - Abstract
Cholangiocarcinoma (CCA) is a cancer with a poor prognosis due to difficulties in diagnosis and limited treatment options, highlighting the urgent need for new targeted therapies. In a clinical setting, we found that leukotriene levels in bile were higher than in serum. Immunohistochemical analysis of surgically resected samples also revealed that CysLT receptor 1 (CysLTR1) was more highly expressed in CCA than in normal bile duct tissue, prompting us to investigate leukotriene as a potential therapeutic target in CCA. In vitro studies using CCA cell lines expressing CysLTR1 showed that leukotriene D4, a major ligand of CysLTR1, promoted cell proliferation, with increased phosphorylation of AKT and extracellular signal-regulated kinase 1/2 (ERK1/2). Additionally, treatment with two clinically available anti-allergic drugs—zileuton, an inhibitor of CysLT formation, and montelukast, a CysLTR1 inhibitor—had inhibitory effects on cell proliferation and migratory capacity, accompanied by the reduced phosphorylation of AKT and ERK1/2. Furthermore, the simultaneous administration of both drugs synergistically enhanced the inhibitory effect on cell proliferation. Our study suggests that use of these drugs may represent a novel approach to treat CCA through drug repositioning.
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- 2024
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7. Potential activity of adiponectin‐expressing regulatory T cells against triple‐negative breast cancer cells through the cell‐in‐cell phenomenon
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Wakana Chikaishi, Toshiya Higashi, Hirokatsu Hayashi, Yuki Hanamatsu, Yusuke Kito, Manabu Futamura, Nobuhisa Matsuhashi, Chiemi Saigo, and Tamotsu Takeuchi
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adiponectin ,adoptive cell therapy ,cell‐in‐cell ,Treg ,triple‐negative breast cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background A population of regulatory T cells (Treg), which reside within thymic nurse cell complexes, express adiponectin and abrogate breast cancer development in transgenic mice. In this study, we examined whether adiponectin‐expressing Treg could impair triple‐negative breast cancer, which is defined by a lack of estrogen receptors, progesterone receptors, and human epidermal growth factor receptor‐2. Methods CD4‐ and CD25‐positive cells were sorted from cultured T lymphocytes of a previously characterized experimental thymic tumor model composed of thymic nurse cells and abundant lymphoid stroma. These sorted cells were examined for FOXP3 and adiponectin immunoreactivity and subsequently exposed to triple‐negative breast cancer MDA‐MB‐157 and ‐231 cells. Results Adiponectin‐expressing Treg were obtained by CD4‐ and CD25‐positive sorting and cell death was induced in triple‐negative breast cancer cells through the cell‐in‐cell phenomenon. Conclusions Adiponectin‐expressing Treg may be candidates for adoptive cell therapy against triple‐negative breast cancer.
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- 2023
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8. Experimental model of micronodular thymic neoplasm with lymphoid stroma
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Riko Niwa, Yuki Hanamatsu, Yusuke Kito, Chiemi Saigo, and Tamotsu Takeuchi
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lymphoid stroma ,micronodular thymic neoplasm with lymphoid stroma ,three‐dimensional cell culture ,thymic epithelial cell ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Micronodular thymic neoplasm with lymphoid stroma (MNT), a subtype of thymic tumor, is histopathologically characterized by micronodular thymic epithelial cell nests with lymphoid stroma. Despite the distinct histopathology of MNT, its pathogenesis remains unclear. Methods In this study, we aimed to examine a thymic tumor harboring thymic epithelial and lymphoid cells in a nonobese diabetic/severe combined immunodeficiency mouse. Results The excised tumor cells were cultured in vitro and comprised epithelial tumor cells and lymphoid cells. During a three‐dimensional cell culture, the epithelial tumor cells formed micronodular cell nests surrounded by lymphoid stroma. Notably, the lymphoid cells underwent apoptosis when they were separated from the epithelial tumor cells. Cutaneous transplantation of the cultured epithelial cells with splenocytes from BALB/c mice led to tumor formation, and these cells demonstrated a histopathology similar to that of human MNT in a nonobese diabetic/severe combined immunodeficiency mouse. Conclusion Given its overlapping features with human MNT, the transplanted tumor could serve as an experimental model of this disease.
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- 2023
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9. An unusual case of hepatosplenic T‐cell lymphoma‐like unclassifiable T/NK‐cell lymphoma accompanied by acute myeloid leukemia
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Shin Lee, Yusuke Kito, Kei Fujita, Hiroto Wakayama, Masaki Kimura, Keisuke Kawashima, Tetsuya Tabata, Tadashi Yoshino, Takeshi Hara, and Hisashi Tsurumi
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acute myeloid leukemia ,CD56 ,hepatosplenic T‐cell lymphoma ,splenomegaly ,TCR rearrangement ,Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
Abstract We describe a case of unclassifiable T/NK‐cell lymphoma with concomitant acute myeloid leukemia (AML). A 73‐year‐old Japanese man was diagnosed with AML by bone marrow smear, but the presence of splenomegaly and liver tumor was incompatible with AML. Splenectomy and hepatic resection were performed to resolve the thrombocytopenia and define the diagnosis. The pathological findings showed sinusoidal involvement of abnormal lymphoid cells that were CD3‐positive but negative for T‐cell receptor (TCR) rearrangement. Our case could not be categorized as hepatosplenic T‐cell lymphoma because of the lack of immunohistological expression of TCR, despite the clinical similarity.
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- 2022
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10. Impact of initial empirical antimicrobial choice and cause of in-hospital death in patients with nursing and healthcare-associated pneumonia (NHCAP): A retrospective study
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Kenichi Kato, Kazunobu Kuwabara, Kiyotaka Ono, Yusuke Kito, Tatsuyoshi Yokoi, Takazumi Yoshida, Keisuke Kato, Masahiro Hirose, Daijo Inaguma, and Takahiko Horiguchi
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nursing and healthcare-associated pneumonia ,pneumonia ,empiric antimicrobial therapy ,Medicine (General) ,R5-920 - Abstract
Objectives: To examine the differences in antimicrobial selection outcomes in nursing and healthcare-associated pneumonia (NHCAP) patients with and without risk factors for drug-resistant pathogen (RDRP) infection, and to identify the cause of in-hospital death after improvement of NHCAP. Methods: We conducted a retrospective analysis of the medical records of hospitalized adult patients with NHCAP. NHCAP patients were divided into the RDRP and non-RDRP groups. The RDRP group was further classified into the narrow and broad subgroups according to the type of empirical antimicrobial agent selected. The difference in mortality between these subgroups was then examined. The cause of all in-hospital deaths was also evaluated. Results: We evaluated 220 patients with NHCAP. There was no difference in mortality between the narrow and broad subgroups (11.8% vs. 15.4%, p=0.655). Among the group with improved NHCAP, 11.3% (n=23/203) died in hospital before discharge. Although the causes of death in patients who improved after NHCAP were diverse, the most common was recurrence of pneumonia. Conclusions: Empirical antimicrobial therapy for NHCAP may not always require selection of broad-spectrum antimicrobial agents, as has been previously reported. Patients with NHCAP may die from other causes, even after NHCAP has improved.
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- 2022
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11. Tumor suppressor effect of an antibody on xenotransplanted sarcomatoid mesothelioma cells
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Masayoshi Hasegawa, Yuki Hanamatsu, Chiemi Saigo, Yusuke Kito, and Tamotsu Takeuchi
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alternatively spliced isoform ,AX10 ,mesothelioma ,sarcolemma‐associated protein ,therapeutic antibody ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background As mesothelioma generally has an unfavorable prognosis, further advances are needed to improve the outcomes in patients with mesothelioma. In the present study, we generated and characterized a monoclonal antibody that could inhibit mesothelioma cell proliferation in a xenotransplantation mouse model. Methods We generated monoclonal antibodies by immunizing mice with cultured mesothelioma cells. These antibodies were then characterized by immunofluorescence staining, immunohistochemical staining, secondary antibody‐drug conjugate assay, antibody inoculation in a xenotransplantation mesothelioma mouse model, and mass spectrometry followed by small interfering RNA (siRNA) analysis. 5' rapid amplification of complementary DNA ends followed by sequencing was performed to deduce the amino acid sequences of the variable regions of the light and heavy chains of AX10. Results An IgG2b κ‐type AX10 antibody against the cell surface membrane of sarcomatoid mesothelioma cells was generated. AX10 immunoreactivity was detected in 12 out of 22 different mesothelioma tissue specimens, but there was little AX10 immunoreactivity in a normal human tissue array. AX10 decreased Matrigel invasion by MPM‐1 cells but did not affect cell proliferation. Notably, AX10 significantly inhibited the proliferation of MPM‐1 cells xenotransplanted into Severe combined immunodeficiency‐Nonobese diabetic mice. Matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry followed by siRNA silencing indicated that AX10 reacted to a unique alternatively spliced isoform of sarcolemma‐associated protein. AX10 is composed of as yet unregistered amino acid sequences in its variable region. Conclusions AX10 could have therapeutic potential for patients with sarcomatoid mesothelioma.
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- 2022
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12. Thymic neuroendocrine cell tumor with blood‑filled caverns
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Yuki Hanamatsu, Chiemi Saigo, Riko Niwa, Yusuke Kito, Hiroyasu Komuro, Koyo Shirahashi, Hisashi Iwata, and Tamotsu Takeuchi
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Thymic carcinoid tumor ,SOX17 ,Angiomatoid neuroendocrine carcinoma ,Endothelial cell ,Pathology ,RB1-214 - Abstract
Three cases of primary thymic neuroendocrine tumors resembling a vascular neoplasm were reported as “Angiomatoid neuroendocrine carcinoma of the thymus.” Recently, we encountered another case of a thymic neuroendocrine cell tumor that grossly mimicked a vascular neoplasm. A man in his early 60 s, who presented with right thoracic pain, was admitted to our hospital. He had a 45 × 35 mm vascular-rich tumor in the thymus and underwent total thymectomy. Histopathological examination revealed that the tumor was composed of many blood‑filled caverns lined with stratified conventional neuroendocrine tumor cells expressing insulinoma-associated protein 1 and synaptophysin immunoreactivity. Notably, the blood-filled caverns were not lined with CD31-positive endothelial cells, as previously reported. By contrast, the caverns were focally lined with cells expressing SRY-Box Transcription Factor 17, which is well characterized to drive the conversion of fibroblast progenitor cells to endothelial cells by its transcriptional property. However, SRY-Box Transcription Factor 17 immunoreactivity was not restricted to the nucleus in blood-filled caverns and was also detected in the nucleus of endothelial cells in tumor vessels in the canonical carcinoid area. Dismaturation of endothelial cells might participate in the angiomatoid features of thymic neuroendocrine cell tumors.
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- 2023
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13. Seasonal variation in the prevalence of Gram-negative bacilli in sputum and urine specimens from outpatients and inpatients
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Yusuke Kito, Kazunobu Kuwabara, Kiyotaka Ono, Kenichi Kato, Tatsuyoshi Yokoi, Kohki Horiguchi, Keisuke Kato, Masahiro Hirose, Tomomi Ohara, Kenta Goto, Yumi Nakamura, Yoshikatsu Koike, and Takahiko Horiguchi
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pseudomonas aeruginosa ,klebsiella pneumoniae ,hospital infection ,seasonality ,Medicine (General) ,R5-920 - Abstract
Objectives: To determine whether the prevalence of gram-negative bacilli (GNB; Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli) in sputum and urine specimens from outpatients and inpatients differed by season and according to temperature and humidity changes. Methods: In this retrospective study, microbiologic data for adult patients from 2008 to 2019 were retrieved from the electronic database of a hospital in Japan. Data were categorized by specimen type (sputum and urine) and specimen collection (outpatient and inpatient). Associations between variables were assessed using Spearman’s rank correlation coefficients. Differences between groups were assessed using Pearson’s chi-square test and analysis of discrete variance. Results: Among inpatients, the frequencies of P. aeruginosa and K. pneumoniae isolation from sputum specimens were higher in summer and autumn. The frequency of P. aeruginosa isolation from urine specimens was higher in autumn. These seasonal trends were observed in specimens from both outpatients and inpatients. No seasonal trend was observed in the frequency of E. coli isolation. Mean monthly temperature was positively correlated with the frequency of isolating P. aeruginosa (r=0.2198, p=0.0081) and K. pneumoniae (r=0.3443, p=0.00002) from sputum as well as with the frequency of isolating K. pneumoniae (r=0.1905, p=0.0222) from urine. Mean monthly humidity was positively correlated with the frequency of isolating K. pneumoniae (r=0.2602, p=0.0016) from sputum. Conclusions: GNB were isolated more frequently in summer and autumn than in other seasons. These seasonal trends were observed for both outpatient and inpatient specimens. Seasonality should be considered for optimal infection control of GNB in hospitals.
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- 2022
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14. Thorahcic SMARCA4-deficient undifferentiated tumors with ganglioneuroma and enchondroma: implications for SLC7A11 and ARID1A expression: a case report
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Yusuke Kito, Keisuke Kawashima, Chiemi Saigo, Masayoshi Hasegawa, Shusuke Nomura, Takuya Mikamo, Yuki Hanamatsu, Yasuhiro Matsuo, and Tamostu Takeuchi
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SMARCA4-deficient thoracic sarcoma ,SMARCA4-deficient non-small cell lung carcinoma ,Thoracic SMARCA4-deficient undifferentiated tumors ,ARID1A ,SLC7A11 ,Ganglioneuroma ,Pathology ,RB1-214 - Abstract
Abstract Background SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A, member 4-deficient thoracic sarcoma (SMARCA4-DTS) is a rare disease that has recently been described as an entity. It is characterized by an aggressive clinical course and specific genetic alterations. As an immunohistological feature, the tumors are deficient in SMARCA4 and SMARCA2 and express sex-determining region Y (SRY)-box 2 (SOX2). Occasionally, there are cases that are less frequent and difficult to distinguish from SMARCA4-deficient non-small cell lung carcinoma (SMARCA4-dNSCLC). Therefore, the 5th edition of the World Health Organization (WHO) classification describes thoracic SMARCA 4-deficient undifferentiated tumors (SMARCA4-UT). In contrast, Carney’s triad is a syndrome that combines three rare soft tissue tumors: gastric leiomyosarcoma, pulmonary chondroma, and extra-adrenal paraganglioma. Protein kinase cAMP-dependent type I regulatory subunit alpha (PRKAR1A) has been proposed as the causative gene. Both diseases are valuable cases; moreover, there have been no previous reports of their coexistence. Case presentation A 43-year-old man visited our hospital because of respiratory distress. Computed tomography revealed a large mass measuring 55 mm in the upper lobe of the right lung and front mediastinum, with metastases in the surrounding lymph nodes. Needle biopsy was performed for diagnosis, and histological examination of the samples revealed monotonous epithelioid-like cells with loose binding and sheet-form proliferation. The tumor cells had distinct nuclei with some rhabdoid-like cells. Immunohistochemical analysis revealed that the tumor cells were positive for AE1AE3, SOX2, CD34, and p53 and negative for SMARCA4 and SMARCA2. The patient died 6 months after admission, without any treatment. Autopsy revealed ganglioneuroma and enchondroma suggestive of an incomplete Carney complex. Conclusion SMARCA4-UT is a rare and recently established disease. While it is difficult to diagnose, it is necessary to distinguish undifferentiated carcinoma, large cell carcinoma, Ewing sarcoma, and epithelioid sarcoma when diagnosing tumors involving the mediastinum. Moreover, cases of SMARCA4-UT with ganglioneuroma and enchondroma are very rare. We discuss and report a case of SMARCA4-UT in which we also examined ARID1A and SLC7A11expression.
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- 2022
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15. Theoretical step approach with 'Three-pillar' device assistance for successful endoscopic transpapillary gallbladder drainage.
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Michihiro Yoshida, Itaru Naitoh, Kazuki Hayashi, Yasuki Hori, Akihisa Kato, Kenta Kachi, Go Asano, Hidenori Sahashi, Tadashi Toyohara, Kayoko Kuno, Yusuke Kito, and Hiromi Kataoka
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Medicine ,Science - Abstract
BackgroundEndoscopic transpapillary gallbladder drainage (ETGBD) has been reported as an alternative procedure for acute cholecystitis but remains a challenging procedure.AimsTo elucidate the efficacy of a strategic approach for ETGBD that utilizes a four-step classification system and the optional use of 'Three-pillar' assistance with the following devices: cholangioscopy (SpyGlass DS, SG), a flex-type guidewire (Flex-GW), and a 3-Fr microcatheter (3-Fr Micro).MethodsA total of 115 patients undergoing ETGBD were studied retrospectively. Characteristics and technical outcomes were compared between conventional ETGBD technique (Classical ETGBD, N = 50) and strategic ETGBD with optional Three-pillar assistance (Strategic ETGBD, N = 65).ResultsSG-assistance (15/65, 23.1%) was as an excellent troubleshooter in Category 1 (failure to identify the cystic duct [CD] orifice) and Category 2 (failure to advance the GW across the CD takeoff due to unfavorable angle). Flex-GW (19/65, 29.2%) worked for Category 3b (failure of GW access to the GB due to multiple tortuosities). 3-Fr Micro (11/65, 16.9%) was effective for Category 3a (failure of GW access to the GB due to CD obstruction) and Category 4 (failure of drainage stent insertion to the GB). The overall technical success rate was significantly higher for Strategic ETGBD (63/65, 96.9%) compared with Classical ETGBD (36/50, 72.0%) (p = 0.0001).ConclusionsStrategic ETGBD, which includes the Three-pillar assistance options of SG in the initial steps, Flex-GW for tortuous CD, and 3-Fr Micro for stenotic CD, achieved a significantly higher success rate than for Classical ETGBD.
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- 2023
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16. Urolithin A targets the AKT/WNK1 axis to induce autophagy and exert anti-tumor effects in cholangiocarcinoma
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Hidenori Sahashi, Akihisa Kato, Michihiro Yoshida, Kazuki Hayashi, Itaru Naitoh, Yasuki Hori, Makoto Natsume, Naruomi Jinno, Kenta Kachi, Go Asano, Tadashi Toyohara, Yusuke Kito, Sudhakar Ammanamanchi, and Hiromi Kataoka
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Urolithin A ,UA ,cholangiocarcinoma ,autophagy ,WNK1 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Urolithin A (UA; 3,8-dihydroxybenzo[c]chromen-6-one), a metabolite generated by intestinal bacteria during the biotransformation of ellagitannins, has gained considerable attention in treating several cancers. Cholangiocarcinoma (CCA) remains one of the most lethal cancers; it grows in a special environment constantly exposed to both blood and bile. Since UA is known to undergo enterohepatic recirculation, we hypothesized that UA might have significant antitumor effects in CCA. Here, we investigated the therapeutic potential of UA in CCA and aimed to elucidate its mechanisms, including autophagy. UA treatment inhibited cell proliferation and induced G2/M phase cell cycle arrest in CCA cells. UA also suppressed cell migration and invasion, but did not cause apoptosis. Furthermore, Western blotting and immunocytochemistry demonstrated increased LC3-II accumulation, while electron microscopy demonstrated induced autophagosomes after UA treatment, suggesting that UA upregulated autophagy in CCA cells. In xenograft mice treated with UA, tumor growth was inhibited with increased LC3-II levels. On the other hand, phospho-kinase array demonstrated downregulation of the AKT/WNK1 pathway. LC3-II expression was elevated in WNK1 knocked down cells, indicating that WNK1 is the key signal for regulating autophagy. Thus, UA exerted antitumor effects by suppressing the AKT/WNK1 signaling pathway and inducing autophagy. In conclusion, UA, a natural, well-tolerated compound, may be a promising therapeutic candidate for advanced CCA.
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- 2022
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17. Evaluation of the pneumococcal urinary antigen test (PUT): a retrospective study
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Tatsuyoshi Yokoi, Kazunobu Kuwabara, Kiyotaka Ono, Yusuke Kito, Kenichi Kato, Keisuke Kato, Masahiro Hirose, Rieko Kondo, and Takahiko Horiguchi
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pneumococcal pneumonia ,urinary antigen testing ,streptococcus pneumoniae ,bacterial pneumonia ,Medicine (General) ,R5-920 - Abstract
Objectives: To determine the usefulness of the pneumococcal urinary antigen test (PUT) and to describe the characteristics of pneumococcal pneumonia. Methods: In this retrospective study, we examined the effects of prior antibiotic treatment, pneumonia onset period, and sputum quality on the results of PUT. Clinical information was collected via medical records from all adult patients who were hospitalized at the Fujita Health University Bantane Hospital with “pneumonia” as a new diagnosis from April 2015 to March 2018. Results: A total of 482 patients with pneumonia were included, of whom 103 had pneumococcal pneumonia. The frequency of PUT positivity did not differ significantly in patients with a pneumonia onset period of ≥3 days compared with those with a period of ≤2 days (P=0.514). Patients with a history of prior antimicrobial therapy had a significantly lower rate of positive sputum culture vs those with no such history (P=0.005); however, PUT positivity in the two groups did not differ significantly (P=0.367). Conclusions: Our results showed that urinary antigen testing for pneumococcal pneumonia is useful for diagnosis regardless of prior antibiotic treatment and time since symptom onset.
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- 2021
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18. Diagnosis and treatment of primary central nervous system lymphoma with the primary lesion in the hypothalamus: a case report
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Ken Takao, Ayaka Tani, Tetsuya Suwa, Yayoi Kuwabara-Ohmura, Kenta Nonomura, Yanyan Liu, Takehiro Kato, Masami Mizuno, Takuo Hirota, Mayumi Enya, Katsumi Iizuka, Yukio Horikawa, Chiemi Saigo, Yusuke Kito, Tatsuhiko Miyazaki, Naoyuki Ohe, Toru Iwama, and Daisuke Yabe
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PCNSL ,Hypothalamus ,Hypopituitarism ,Case report ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Abstract Background Primary central nervous system lymphoma is a rare extra-nodal lymphoma of the central nervous system. Primary central nervous system lymphoma lesions usually appear in the vicinity of the ventricle, and there are few reports of primary central nervous system lymphoma with hypothalamic-pituitary lesions. Case presentation We treated a 56-year-old male with primary central nervous system lymphoma with the primary lesion in the hypothalamus, which was found by magnetic resonance imaging after sudden onset of endocrinological abnormalities. Initially, he was hospitalized to our department for hyponatremia. Endocrinological examination in conjunction with head magnetic resonance imaging and endoscopic biopsy revealed hypothalamic hypopituitarism and tertiary hypoadrenocorticism caused by a rapidly growing, diffuse large B-cell lymphoma in the hypothalamus. Remission of the tumor was achieved by high-dose methotrexate with whole brain radiotherapy, and some of the hormone responses were normalized. Conclusions While primary central nervous system lymphoma is rare, it is important to note that hypopituitarism can result and that the endocrinological abnormalities can be partially restored by its remission.
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- 2021
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19. Overexpression of JAG2 is related to poor outcomes in oral squamous cell carcinoma
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Kiichi Hatano, Chiemi Saigo, Yusuke Kito, Toshiyuki Shibata, and Tamotsu Takeuchi
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JAG2 ,Notch ligand ,oral squamous cell carcinoma ,prognosis ,Dentistry ,RK1-715 - Abstract
Abstract Objectives JAG2 is one of Notch ligands, which recently appear to exert various carcinogenesis. In the present study, we aimed to unveil the relation of JAG2 expression and clinicopathological features in oral squamous cell carcinoma (OSCC). Materials and Methods We examined JAG2 expression in OSCC plus adjacent nontumorous epithelia in eight patients. Ninety‐one OSCC tissue specimens were immunohistochemically stained with specific antibodies to JAG2. The immunoreactivities of JAG2 were correlated with clinicopathological factors, including the prognosis of patients. Chi‐square test, Kaplan–Meier survival, and Cox proportional hazard analysis were used to determine the statistical value of JAG2 expression in OSCC. Results JAG2 mRNA expression was much expressed in OSCC tissues compared with adjacent tissue specimens in five of eight patients. JAG2 immunoreactivity was found at invasion front in 31 of 91 OSCC. JAG2 immunoreactivity was significantly associated with age, less than 50 years old of patients (P = .048). Kaplan–Meier analysis demonstrated that the patients with JAG2 immunoreactvty have a short overall survival. With the Cox proportional hazard regression mode, the independent factors predictive of poor overall survival included JAG2 immunoreactivity (P < .05). Conclusions The present findings suggest that JAG2 overexpression, especially at the cancer invasion front, has potential prognostic value.
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- 2020
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20. Submucosal tumor-like esophageal cancer mimicking a cutaneous sweat gland carcinoma: A case report
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Chiemi Saigo, Yuki Hanamatsu, Masayoshi Hasegawa, Shusuke Nomura, Takuya Mikamo, Yusuke Kito, Itaru Yasufuku, Hideharu Tanaka, Kazuhiro Yoshida, and Tamotsu Takeuchi
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Esophageal cancer ,Esophageal submucosal gland ,SMT-like tumor ,Squamoid ductal eccrine carcinoma ,Sweat gland tumor of the skin ,Pathology ,RB1-214 - Abstract
Esophageal cancer rarely grows as an intramural tumor with an intact surface epithelium. A previous report described submucosal tumor-like esophageal cancer that had an overlapping morphological phenotype similar to that of a cutaneous sweat gland tumor. Here, we encountered another case of submucosal esophageal cancer mimicking skin adnexal carcinoma. A man in his early 60s who presented with difficulty in swallowing was admitted to our hospital. He had a 30 × 30 mm submucosal tumor in the abdominal esophagus and underwent partial esophagogastrectomy. Histopathological examination revealed that the tumor was composed of cord-like squamoid cell nests that was partially accompanied by gland structure in the inner layer, as found in cutaneous squamoid ductal eccrine carcinoma. The tumor exhibited CK7 immunoreactivity as did the neighboring dilated ducts of the esophageal gland. We describe a unique submucosal tumor-like esophageal cancer that had overlapping morphological features with squamoid ductal eccrine carcinoma and putatively originated in the esophageal submucosal gland ducts.
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- 2021
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21. Incidental cytokeratin-positive interstitial reticulum cell tumor of the lymph node accompanied by breast cancer: Status of YAP/TAZ expression in tumor cells
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Chiemi Saigo, Yusuke Kito, Masayoshi Hasegawa, Shusuke Nomura, Takuya Mikamo, Yuki Hanamatsu, Ryutaro Mori, Manabu Futamura, Kazuhiro Yoshida, and Tamotsu Takeuchi
- Subjects
Cytokeratin-positive interstitial reticulum cell tumor ,YAP/TAZ ,Fibroblastic reticular cells ,Lymphoid stromal tumor ,Pathology ,RB1-214 - Abstract
Approximately 24 cases of cytokeratin-positive interstitial reticulum cell tumors have been reported in English literature. In this paper, we report a case of cytokeratin-positive interstitial reticulum cell tumor that was localized in the parafollicular region of the axillary lymph node and was an incidental finding in a patient with breast cancer. A 50 year-old woman underwent a mastectomy and lymph node dissection following a preoperative diagnosis of bilateral breast cancer. Histopathological examination revealed that the patient had non-invasive and invasive ductal carcinoma in the right and left mammary glands. Although we could not detect breast cancer metastasis in the lymph nodes, one of eight right axillary lymph nodes harbored an 11 × 8 mm discohesive sheet of large polygonal epithelioid tumor cells in the parafollicular area. Tumor cells were immunoreactive for AE1/AE3, CAM5.2, CK18 and l-caldesmon and negative for CD1a, CD21, CD23, CD31, CD68, and S‑100 protein markers. Based on these findings, the lesion was diagnosed as a cytokeratin-positive interstitial reticulum cell tumor of the lymph node. Notably, this early-stage cytokeratin-positive interstitial reticulum cell tumor demonstrated focal nuclear YAP/TAZ proto-oncogene products, which were recently identified as critical for differentiation, maturation, and function of fibroblastic reticular cells. To the best of our knowledge, this is the first report to describe nuclear YAP/TAZ immunoreactivity in cytokeratin-positive interstitial reticulum cell tumors.
- Published
- 2021
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22. A unique case of splenic tumor exhibiting a serous carcinoma phenotype
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Keisuke Kawashima, Yusuke Kito, Koya Tochii, Masaki Kimura, Taro Takeda, Takafumi Sekino, Tsuneko Ikeda, and Tamotsu Takeuchi
- Subjects
Medicine (General) ,R5-920 - Abstract
The spleen has no epithelial element; thus, primary carcinoma of the spleen is quite rare. We present the case of a patient with serous carcinoma of the spleen. A 76-year-old woman with no significant medical history presented with a huge lesion in the spleen. Except this lesion, clinical examination, including imaging examination, revealed no remarkable findings. She underwent excision of the spleen for treatment and diagnosis. Postoperative pathological examination revealed neoplastic cells with pleomorphic and hyperchromatic nuclei, prominent nucleoli, and frequent mitotic activity. The neoplastic cells exhibited a papillary pattern with psammoma bodies. Immunohistochemistry showed positivity for cytokeratin 7, PAX-8, WT-1, p16, p53, and Ber-EP4 and negativity for cytokeratin 20, thyroid transcription factor-1, carcinoembryonic antigen, CD10, estrogen receptor, calretinin, D2-40, intelectin-1, and sialylated HEG1. We inferred that this tumor was a primary splenic serous carcinoma. Serous tubal intraepithelial carcinoma is the plausible origin of most pelvic serous carcinomas. However, the origin of serous carcinoma of the spleen remains unknown. We speculated that endosalpingiosis might be the origin of the tumor.
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- 2021
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23. Combination of Clptm1L and TMEM207 Expression as a Robust Prognostic Marker in Oral Squamous Cell Carcinoma
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Kimika Hano, Kiichi Hatano, Chiemi Saigo, Yusuke Kito, Toshiyuki Shibata, and Tamotsu Takeuchi
- Subjects
OSCC ,prognosis ,TMEM207 ,CLPTM1L ,ER-stress ,WWOX ,Dentistry ,RK1-715 - Abstract
Overexpression of Cleft Lip and Palate Transmembrane 1-Like (Clptm1L) confers cancer cell survival through the endoplasmic reticulum (ER) stress survival signaling pathway, while TMEM207 impairs the tumor suppressor function of WW domain containing oxidoreductase (WWOX), which sensitizes cancer cells to ER stress-induced apoptosis. In the present study, we examined whether these two ER stress-related proteins, Clptm1L and TMEM207, could be prognostic markers in oral squamous cell carcinoma (OSCC). Immunohistochemical staining using specific antibodies to Clptm1L or TMEM207 revealed that 31 of 89 tissue specimens exhibited concomitant expression of Clptm1L and TMEM207 at the cancer invasion front. A Kaplan–Meier plot of the patient survival curve followed by a log-rank test revealed that the coexpression of Clptm1L and TMEM207 was significantly associated with poor outcome in patients with OSCC (P = 0.00252). Coexpression of Clptm1L and TMEM207 was closely related to lymph node metastasis (P=0.000574). Both univariate and multivariate analyses demonstrated that coexpression of Clptm1L and TMEM207 predicted the poor prognosis of the patients with OSCC. The present study indicated that the double positive Clptm1L and TMEM207 immunoreactivity was closely related to lymph node metastasis with prognostic value in patients with OSCC.
- Published
- 2021
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24. A unique transgenic mouse model exhibiting a myeloproliferative disease-like phenotype
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Yusuke Kito, Yuki Hanamatsu, Keisuke Kawashima, Chiemi Saigo, and Tamotsu Takeuchi
- Subjects
myeloproliferative diseases ,mouse model ,tmem207 ,atg4b ,Science ,Biology (General) ,QH301-705.5 - Abstract
Transmembrane protein 207 (TMEM207) is an important molecule involved in invasiveness of gastric signet ring cell carcinoma. To understand the pathobiological effects of TMEM207, we generated thirteen transgenic mouse lines, designated C57BL/6-Tg (ITF-TMEM207), where mouse TMEM207 is expressed heterotrophically, regulated by the proximal promoter of the murine intestinal trefoil factor (ITF) gene (also known as Tff3). A C57BL/6-Tg (ITF-TMEM207) mouse line unexpectedly exhibited a high incidence of a spontaneous condition resembling myeloproliferative disease-like phenotype. Increased numbers of CD117+ cells and appearance of dysplastic myeloid cells in bone marrow were observed. These histopathological features suggested human myeloproliferative disease or its precursor manifestations, and were found in almost all mice within 1 year. TMEM207 immunoreactivity was identified in megakaryocytes and erythroblasts of the transgenic mice. The ITF-TMEM207 construct was inserted into Atg4b on murine chromosome 1. Myeloproliferative disease was not observed in other C57BL/6-Tg (ITF-TMEM207) transgenic mouse lines. However, although several other genetically manipulated animal models of myeloproliferative disease and Atg4b knockout mice exist, this mouse line harboring a mutated Atg4b gene, and with overexpression of TMEM207 protein, has not been reported as a model of myeloproliferative disease to date. The present study demonstrated that the C57BL/6-Tg (ITF-TMEM207) mouse may be a valuable model for improved understanding of human myeloproliferative disease.
- Published
- 2019
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25. A Case of Gastric Amphicrine Signet-Ring Cell Carcinoma
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Yuki Hanamatsu, Chiemi Saigo, Nami Asano, Yusuke Kito, Kazuhiko Nakada, Yohei Takeda, and Tamotsu Takeuchi
- Subjects
Pathology ,RB1-214 - Abstract
“Amphicrine” (in Greek, amphi - means “both” or “double”) refers to cells that synchronously exhibit the endocrine and exocrine phenotypes. Gastric amphicrine carcinoma is very rare, and only a few case reports are found in the English literature; thus, its pathobiological features remain unclear. Here, we report a case of amphicrine gastric carcinoma. A woman in her sixth decade of life presented with anemia and underwent upper endoscopy, followed by histopathological examination of biopsy specimens. She appeared to have gastric cancer with a tumor measuring 5.0 cm × 4.0 cm in size. Subsequently, the patient underwent total gastrectomy with lymph node dissection. Histopathological examination revealed a poorly cohesive carcinoma that sparsely coexisted with signet-ring cell carcinoma cells with regional lymph node metastasis. Interestingly, synaptophysin immunoreactivity with the coexistence of Alcian blue was found in individual signet-ring cell carcinoma cells. Furthermore, the present amphicrine carcinoma cells immunohistochemically expressed CD44 variant 9, a functional cancer stem cell marker. We believe that the present case findings may support the idea of multipotent stem cells being an origin of amphicrine gastric cancers.
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- 2019
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26. Cancerous Protein Network That Inhibits the Tumor Suppressor Function of WW Domain-Containing Oxidoreductase (WWOX) by Aberrantly Expressed Molecules
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Chiemi Saigo, Yusuke Kito, and Tamotsu Takeuchi
- Subjects
WW domain ,WWOX ,PPxY motif ,cancer ,aerobic glycolysis ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Recent findings indicate that the WW domain-containing oxidoreductase (WWOX) is a tumor suppressor protein that contains two N-terminal WW domains and a central short-chain dehydrogenase/reductase domain. WWOX protein mediates multiple signaling networks that suppress carcinogenesis through binding of its first WW domain to various cancer-associated proteins, i.e., p73, AP-2γ, and others. Although the tumor suppressor property of WWOX is inarguable, WWOX is not inactivated in the manner characteristic of the canonical Knudson hypothesis. Impairment of both alleles of WWOX is thought to be a rare event, only occurring in a few cancer cell lines. How is the tumor suppressor function of WWOX impaired in cancer cells? Recent advances highlight that a small transmembrane protein possessing a PPxY motif, called TMEM207, and its relatives are aberrantly expressed in various cancer cells and hinder the tumor suppressor function of WWOX through inhibiting its WW domain. Here, we review the recent findings related to the pathobiological properties of TMEM207 and its relatives based on clinicopathological and experimental pathological studies.
- Published
- 2018
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27. Therapeutic potential of CPI-613 for targeting tumorous mitochondrial energy metabolism and inhibiting autophagy in clear cell sarcoma.
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Yuki Egawa, Chiemi Saigo, Yusuke Kito, Toshiaki Moriki, and Tamotsu Takeuchi
- Subjects
Medicine ,Science - Abstract
Clear cell sarcoma (CCS) is an aggressive type of soft tissue tumor that is associated with high rates of metastasis. In the present study, we found that CPI-613, which targets tumorous mitochondrial energy metabolism, induced autophagosome formation followed by lysosome fusion in HS-MM CCS cells in vitro. Interestingly, CPI-613 along with chloroquine, which inhibits the fusion of autophagosomes with lysosomes, significantly induced necrosis of HS-MM CCS cell growth in vitro. Subsequently, we established a murine orthotropic metastatic model of CCS and evaluated the putative suppressive effect of a combination of CPI-613 and chloroquine on CCS progression. Injection of HS-MM into the aponeuroses of the thigh, the most frequently affected site in CCS, resulted in massive metastasis in SCID-beige mice. By contrast, intraperitoneal administration of CPI-613 (25 mg/kg) and chloroquine (50 mg/kg), two days a week for two weeks, significantly decreased tumor growth at the injection site and abolished metastasis. The present results imply the inhibitory effects of a combination of CPI-613 and chloroquine on the progression of CCS.
- Published
- 2018
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28. Transgenic mouse model of cutaneous adnexal tumors
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Yusuke Kito, Chiemi Saigo, Kurabayashi Atsushi, Furihata Mutsuo, and Takeuchi Tamotsu
- Subjects
Cutaneous adnexal tumor ,Mouse model ,TMEM207 ,Medicine ,Pathology ,RB1-214 - Abstract
TMEM207 was first characterized as being an important molecule for the invasion activity of gastric signet-ring cell carcinoma cells. In order to unravel the pathological properties of TMEM207, we generated several transgenic mouse lines, designated C57BL/6-Tg (ITF-TMEM207), in which murine TMEM207 was ectopically expressed under a truncated (by ~200 bp) proximal promoter of the murine intestinal trefoil factor (ITF) gene (also known as Tff3). Unexpectedly, a C57BL/6-Tg (ITF-TMEM207) mouse line exhibited a high incidence of spontaneous intradermal tumors with histopathological features that resembled those of various human cutaneous adnexal tumors. These tumors were found in ~14% female and 13% of male 6- to 12-month-old mice. TMEM207 immunoreactivity was found in hair follicle bulge cells in non-tumorous skin, as well as in cutaneous adnexal tumors of the transgenic mouse. The ITF-TMEM207 construct in this line appeared to be inserted to a major satellite repeat sequence at chromosome 2, in which no definite coding molecule was found. In addition, we also observed cutaneous adnexal tumors in three other C57BL/6-Tg (ITF-TMEM207) transgenic mouse lines. We believe that the C57BL/6-Tg (ITF-TMEM207) mouse might be a useful model to understand human cutaneous adnexal tumors.
- Published
- 2014
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29. Cervical Symmetric Dumbbell Ganglioneuromas Causing Severe Paresis
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Akira Hioki, Kei Miyamoto, Yoshinobu Hirose, Yusuke Kito, Kazunari Fushimi, and Katsuji Shimizu
- Subjects
Ganglioneuroma ,Symmetric ,Dumbbell tumor ,Cervical spine ,Medicine - Abstract
We report an extremely rare case with bilateral and symmetric dumbbell ganglioneuromas of the cervical spine in an elderly patient. A 72-year-old man came by ambulance to our hospital due to progressive incomplete paraplegia. Magnetic resonance imaging demonstrated bilateral symmetric dumbbell tumors at the C1/2 level. We performed total resection of the intracanalar tumor, aiming at complete decompression of the spinal cord, and partial and subtotal resection of foraminal outside portions. Histopathological examination of the surgical specimen indicated the tumor cells to be spindle cells with the presence of ganglion cells and no cellular pleomorphism, suggesting a diagnosis of ganglioneuroma. Although the surgery was not curative, the postoperative course was uneventful and provided a satisfactory outcome. This is the fourth known case of cervical ganglioneuromas of the bilateral symmetric dumbbell type.
- Published
- 2014
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30. Expression of beclin-1 in the microenvironment of invasive ductal carcinoma of the breast: correlation with prognosis and the cancer-stromal interaction.
- Author
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Akemi Morikawa, Tamotsu Takeuchi, Yusuke Kito, Chiemi Saigo, Takuji Sakuratani, Manabu Futamura, and Kazuhiro Yoshida
- Subjects
Medicine ,Science - Abstract
We examined the pathobiological properties of beclin-1, which is a key regulator of autophagosome formation in invasive ductal carcinoma of the breast, with a particular focus on the cancer microenvironment. Immunohistochemistry demonstrated that cancer cells and stromal mesenchymal cells expressed beclin-1 in 68 and 38 of 115 invasive ductal cancers, respectively. Expression of beclin-1 in cancer or stromal cells alone did not correlate with patient prognosis. In contrast, loss of beclin-1 in cancer cells and overexpression in stromal mesenchymal cells was associated with local cancer recurrence, postoperative lymph node metastasis, and a poor disease-free survival rate. A comprehensive gene expression analysis was performed on a co-culture of breast cancer cells and mesenchymal stromal cells, that latter of which either expressed beclin-1 or was depleted of beclin-1 by siRNA. Notably, siRNA-mediated downregulation of beclin-1 in mesenchymal cells co-cultured with breast cancer cells decreased the levels of various pro-inflammatory cytokines, their receptors, and collagen receptors. Quantitative reverse transcription polymerase chain reaction analysis confirmed that reduction of stromal beclin-1 expression decreased the expression of IL-1β and collagen receptor discoidin domain receptor 2 (DDR2). Microenvironmental IL-1β is believed to play an important role in tumor invasion. Recent work has also indicated that overexpression of DDR2 contributes to breast cancer invasion and lymph node metastasis. Taken together, these findings indicate beclin-1 expression in the stroma might be important for shaping the breast cancer microenvironment and thus could be a potent molecular target in patients with invasive ductal carcinoma of the breast.
- Published
- 2015
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31. Interaction support systems between teachers and visual content for effortless creation of program visualization.
- Author
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Koichi Yamashita, Miyu Suzuki, Yusuke Kito, Yusuke Suzuki, Satoru Kogure, Yasuhiro Noguchi, Raiya Yamamoto, Tatsuhiro Konishi, and Yukihiro Itoh
- Published
- 2023
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32. The great results of our unique intrahospital cooperation alert system for positive hepatitis C test results
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Yusuke Kito, Takashi Mizushima, Kenji Urakabe, Ryo Ishihara, Akihisa Minowa, Yuta Suzuki, Kenichi Haneda, Hironobu Tsukamoto, and Fumihiro Okumura
- Subjects
Hepatology - Published
- 2022
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33. Two synchronous solid pseudopapillary neoplasms detected only by EUS
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Kenichi HANEDA, Yusuke KITO, Yuta SUZUKI, Norio OKUMURA, Masaki KAJIKAWA, Kazuko WATANABE, and Fumihiro OKUMURA
- Published
- 2021
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34. Two cases of steroid-resistant nivolumab-induced sclerosing cholangitis
- Author
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Yuta Suzuki, Kazuko Watanabe, Yusuke Kito, Kenji Urakabe, Takashi Mizushima, Hironobu Tsukamoto, Kenichi Haneda, Fumihiro Okumura, Yasuaki Fujita, Ryo Ishihara, and Akihisa Minowa
- Subjects
Hepatology ,business.industry ,Cancer research ,Medicine ,Nivolumab ,business ,Steroid resistant - Published
- 2021
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35. Experimental model of micronodular thymic neoplasm with lymphoid stroma
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Riko Niwa, Yuki Hanamatsu, Yusuke Kito, Chiemi Saigo, and Tamotsu Takeuchi
- Subjects
Pulmonary and Respiratory Medicine ,Oncology ,General Medicine - Abstract
Micronodular thymic neoplasm with lymphoid stroma (MNT), a subtype of thymic tumor, is histopathologically characterized by micronodular thymic epithelial cell nests with lymphoid stroma. Despite the distinct histopathology of MNT, its pathogenesis remains unclear.In this study, we aimed to examine a thymic tumor harboring thymic epithelial and lymphoid cells in a nonobese diabetic/severe combined immunodeficiency mouse.The excised tumor cells were cultured in vitro and comprised epithelial tumor cells and lymphoid cells. During a three-dimensional cell culture, the epithelial tumor cells formed micronodular cell nests surrounded by lymphoid stroma. Notably, the lymphoid cells underwent apoptosis when they were separated from the epithelial tumor cells. Cutaneous transplantation of the cultured epithelial cells with splenocytes from BALB/c mice led to tumor formation, and these cells demonstrated a histopathology similar to that of human MNT in a nonobese diabetic/severe combined immunodeficiency mouse.Given its overlapping features with human MNT, the transplanted tumor could serve as an experimental model of this disease.
- Published
- 2022
36. Clinical impact of bile-derived exosomal microRNAs as novel diagnostic and prognostic biomarkers for biliary tract cancers
- Author
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Michihiro Yoshida, Hiroshi Yukawa, Kazuki Hayashi, Itaru Naitoh, Katsuyuki Miyabe, Yasuki Hori, Makoto Natsume, Naruomi Jinno, Akihisa Kato, Kenta Kachi, Go Asano, Hidenori Sahashi, Tadashi Toyohara, Kayoko Kuno, Yusuke Kito, Hiromu Kondo, Atsuyuki Hirano, Fumihiro Okumura, Kaiki Anbe, Yoshinobu Baba, Hiromi Kataoka, and Yasuhito Tanaka
- Subjects
Cancer Research ,MicroRNAs ,Biliary Tract Neoplasms ,Oncology ,Gene Expression Profiling ,Biomarkers, Tumor ,Humans ,Bile ,General Medicine ,Prognosis ,Exosomes ,Biomarkers - Abstract
Sampling of bile juice during endoscopic retrograde cholangiopancreatography (ERCP) has potential benefit of being amenable to the identification of novel biomarkers in liquid biopsy. This study reports the results of a global investigation of exosomal microRNAs (miRNAs) in bile to identify potential biomarkers for biliary tract cancers (BTCs). Eighty-eight bile samples collected during ERCP (45 BTC and 43 noncancer control samples) were enrolled in this study. Eleven BTC samples and nine control samples were assigned as the discovery set. Exosomes in bile and serum samples were collected using a glass membrane column with size-controlled macroporous glass (MPG), and exosomal miRNA expression profiles were evaluated using comprehensive miRNA microarray analysis (3D-Gene). For validation, exosomal miRNA in the bile samples of 34 BTCs and 34 controls were comprehensively evaluated using 3D-Gene. In the discovery set, eight exosomal miRNAs in bile were identified as significant aberrant expression markers, while no miRNA with aberrant expression in serum was identified. In a comparison of the discovery and validation sets, miR-451a and miR-3619-3p were identified as reproducible upregulated markers, and the combination of the two bile miRNAs showed an excellent area under the curve (0.819) value for diagnosing BTCs. In addition, high miR-3619-3p expression in bile reflects poorer prognosis of BTCs (hazard ratio = 2.89). The MPG-extracted exosomal miRNAs in bile aspirated during ERCP provide a convenient new approach for diagnosing biliary diseases. Bile-derived miRNA analysis with miR-451a and miR-3619-3p represents a potentially valuable diagnostic strategy for identifying BTCs as well as a predictive indicator of BTC prognosis.
- Published
- 2022
37. Use of Endoscopic Scraper and Cell Block Technique as a Replacement for Conventional Brush for Diagnosing Malignant Biliary Strictures
- Author
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Akihisa Kato, Hiroyuki Kato, Itaru Naitoh, Kazuki Hayashi, Michihiro Yoshida, Yasuki Hori, Kenta Kachi, Go Asano, Hidenori Sahashi, Tadashi Toyohara, Kayoko Kuno, Yusuke Kito, Satoru Takahashi, and Hiromi Kataoka
- Subjects
Cancer Research ,Oncology ,endoscopic scraper ,cell block ,biliary strictures ,conventional brush - Abstract
Histological evidence is essential for diagnosing malignant biliary strictures. However, conventional brush cytology remains the primary method used worldwide, despite its low diagnostic sensitivity and accuracy, as it is technically easy, rapid, and cost-effective. The aim of this study was to evaluate the diagnostic performance of a recently introduced endoscopic scraper, the simplicity of which is comparable to that of a conventional brush, by comparing diagnostic yields and the number of collected cells. The sensitivity of the endoscopic scraper when using the cell block technique was significantly higher than when using brush cytology or a brush with the cell block technique (53.6% vs. 30.9%, p < 0.001; 53.6% vs. 31.6%, p = 0.024, respectively). Quantitative digital image analysis of cell block sections revealed that the median number of cells obtained with the endoscopic scraper was significantly higher than when using the brush (1917 vs. 1014 cells, p = 0.042). Furthermore, seven cases (8.3%; 7/84) were diagnosed by immunohistochemical analysis of the cell block section obtained from the endoscopic scraper. Given its simplicity and greater capacity for sample acquisition, use of the endoscopic scraper in conjunction with the cell block technique could replace brush cytology for the histological diagnosis of malignant biliary strictures.
- Published
- 2022
38. A Case of Concomitant Pancreatic Ductal Adenocarcinoma and Type 1 Autoimmune Pancreatitis: A Potential Issue in the Diagnosis of Carcinoma by Endoscopic Ultrasound-guided Fine-needle Biopsy
- Author
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Kenta Kachi, Itaru Naitoh, Tesshin Ban, Kazuki Hayashi, Michihiro Yoshida, Yasuki Hori, Makoto Natsume, Akihisa Kato, Yusuke Kito, Kenta Saito, Yoichi Matsuo, Hiroyuki Kato, Aya Naiki-Ito, Satoru Takahashi, Kenji Notohara, and Hiromi Kataoka
- Subjects
Internal Medicine ,General Medicine - Abstract
We herein report a 64-year-old man with concomitant pancreatic ductal adenocarcinoma (PDAC) and type 1 autoimmune pancreatitis (AIP). An endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) from the pancreatic head mass revealed level 2 histology of AIP and atypical glands. We diagnosed definitive focal AIP using the clinical diagnostic criteria. Computed tomography revealed that the pancreatic mass had not been reduced by steroid therapy. Surgery was performed after a histological PDAC diagnosis was made via a transpapillary biliary biopsy. The resected specimen revealed PDAC associated with AIP. It is important to consider the cooccurrence of PDAC and AIP even if the histological diagnosis via an EUS-FNB is AIP.
- Published
- 2022
39. Human colorectal cancer-associated carbohydrate antigen on annexin A2 protein
- Author
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Hideharu Tanaka, Yoshinori Iwata, Tamotsu Takeuchi, Kazuhiro Yoshida, Chiemi Saigo, Yusuke Kito, and Itaru Yasufuku
- Subjects
Glycosylation ,Colorectal cancer ,medicine.drug_class ,Fluorescent Antibody Technique ,Monoclonal antibody ,Epitope ,Epitopes ,03 medical and health sciences ,0302 clinical medicine ,Antigen ,Drug Discovery ,Biomarkers, Tumor ,medicine ,Humans ,Antigens, Tumor-Associated, Carbohydrate ,Amino Acid Sequence ,Annexin A2 ,Genetics (clinical) ,biology ,Chemistry ,Antibodies, Monoclonal ,Cancer ,Prognosis ,medicine.disease ,Immunohistochemistry ,Survival Analysis ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,biology.protein ,Cancer research ,Molecular Medicine ,Antibody ,Colorectal Neoplasms ,030215 immunology - Abstract
Cancer-associated antigens are not only a good marker for monitoring cancer progression but are also useful for molecular target therapy. In this study, we aimed to generate a monoclonal antibody that preferentially reacts with colorectal cancer cells relative to noncancerous gland cells. We prepared antigens composed of HT-29 colorectal cancer cell lysates that were adsorbed by antibodies to sodium butyrate-induced enterocytically differentiated HT-29 cells. Subsequently, we generated a monoclonal antibody, designated 12G5A, which reacted with HT-29 colon cancer cells, but not with sodium butyrate-induced differentiated HT-29 cells. Immunohistochemical staining revealed 12G5A immunoreactivity in all 73 colon cancer tissue specimens examined at various degrees, but little or no immunoreactivity in noncancerous gland cells. Notably, high 12G5A immunoreactivity, which was determined as more than 50% of colon cancer cells intensively stained with 12G5A antibody, exhibited significantly higher association with a poor overall survival rate of patients with colorectal cancer (P = 0.0196) and unfavorable progression-free survival rate of patients with colorectal cancer (P = 0.0418). Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, si-RNA silencing analysis, enzymatic deglycosylation, and tunicamycin treatment revealed that 12G5A recognized the glycosylated epitope on annexin A2 protein. Our findings indicate that 12G5A identified a cancer-associated glycosylation epitope on annexin A2, whose expression was related to unfavorable colorectal cancer behavior. KEY MESSAGE: • 12G5A monoclonal antibody recognized a colorectal cancer-associated epitope. • 12G5A antibody recognized the N-linked glycosylation epitope on annexin A2. • 12G5A immunoreactivity was related to unfavorable colorectal cancer behavior.
- Published
- 2021
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40. Diagnosis and treatment of primary central nervous system lymphoma with the primary lesion in the hypothalamus: a case report
- Author
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Masami Mizuno, Katsumi Iizuka, Naoyuki Ohe, Toru Iwama, Yusuke Kito, Chiemi Saigo, Yukio Horikawa, Yayoi Kuwabara-Ohmura, Yanyan Liu, Kenta Nonomura, Tatsuhiko Miyazaki, Tetsuya Suwa, Takehiro Kato, Ayaka Tani, Takuo Hirota, Daisuke Yabe, Ken Takao, and Mayumi Enya
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Antimetabolites, Antineoplastic ,Hormone Replacement Therapy ,Endocrinology, Diabetes and Metabolism ,Central nervous system ,Hypothalamus ,Hypopituitarism ,Endocrine System Diseases ,lcsh:Diseases of the endocrine glands. Clinical endocrinology ,Central Nervous System Neoplasms ,03 medical and health sciences ,0302 clinical medicine ,Adrenal Cortex Hormones ,hemic and lymphatic diseases ,Case report ,medicine ,Humans ,PCNSL ,lcsh:RC648-665 ,medicine.diagnostic_test ,business.industry ,Primary central nervous system lymphoma ,Magnetic resonance imaging ,General Medicine ,Chemoradiotherapy ,Middle Aged ,medicine.disease ,Combined Modality Therapy ,Magnetic Resonance Imaging ,Lymphoma ,medicine.anatomical_structure ,Methotrexate ,Treatment Outcome ,030220 oncology & carcinogenesis ,Lymphoma, Large B-Cell, Diffuse ,Hypothalamic Neoplasms ,Hyponatremia ,business ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Background Primary central nervous system lymphoma is a rare extra-nodal lymphoma of the central nervous system. Primary central nervous system lymphoma lesions usually appear in the vicinity of the ventricle, and there are few reports of primary central nervous system lymphoma with hypothalamic-pituitary lesions. Case presentation We treated a 56-year-old male with primary central nervous system lymphoma with the primary lesion in the hypothalamus, which was found by magnetic resonance imaging after sudden onset of endocrinological abnormalities. Initially, he was hospitalized to our department for hyponatremia. Endocrinological examination in conjunction with head magnetic resonance imaging and endoscopic biopsy revealed hypothalamic hypopituitarism and tertiary hypoadrenocorticism caused by a rapidly growing, diffuse large B-cell lymphoma in the hypothalamus. Remission of the tumor was achieved by high-dose methotrexate with whole brain radiotherapy, and some of the hormone responses were normalized. Conclusions While primary central nervous system lymphoma is rare, it is important to note that hypopituitarism can result and that the endocrinological abnormalities can be partially restored by its remission.
- Published
- 2021
41. Anti-fibrotic properties of an adiponectin paralog protein, C1q/TNF-related protein 6 (CTRP6), in diffuse gastric adenocarcinoma
- Author
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Yoshinori Iwata, Yusuke Kito, Chiemi Saigo, Tamotsu Takeuchi, Kazuhiro Yoshida, and Itaru Yasufuku
- Subjects
0301 basic medicine ,Stromal cell ,03 medical and health sciences ,diffuse-type gastric cancer ,0302 clinical medicine ,desmoplastic reaction ,Fibrosis ,medicine ,Fibroblast ,business.industry ,digestive, oral, and skin physiology ,cancer stromal microenvironment ,Cancer ,medicine.disease ,myofibroblast ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,Immunohistochemistry ,Tumor necrosis factor alpha ,CTRP6 ,business ,Myofibroblast ,Research Paper - Abstract
Patients with advanced gastric cancer, especially diffuse-type gastric cancer, which is often accompanied by stromal fibrosis, commonly exhibit a poor prognosis. This study was designed to unravel the potential roles of C1q/TNF-related protein 6 (CTRP6) in the fibrotic cancer microenvironment of diffuse-type gastric adenocarcinoma. A total of 49 diffuse-type gastric cancer samples were evaluated in this study, and 23 of these samples exhibited focal CTRP6 immunoreactivity. CTRP6 immunoreactivity was found to be correlated with favorable survival outcomes, in terms of both overall and relapse-free survival rates, but this trend did not reach significance (P = 0.15). By contrast, CTRP6 immunoreactivity was significantly correlated with relapse-free survival rates in patients with diffuse-type gastric cancer at a distal site (P = 0.028). Notably, most gastric cancer cells at the cancer invasive front were CTRP6 negative, especially in areas of robust fibrosis. Double immunohistochemical staining demonstrated an inverse expression profile for CTRP6 and the activated fibroblast marker alpha smooth muscle actin (α-sma) in stromal and gastric cancer cells at the cancer invasion front. The addition of recombinant CTRP6 protein attenuated the TGF-β-induced α-sma expression in cultured human fibroblasts but did not alter the proliferation rate or Matrigel-invasion activity of the cultured gastric cancer cells. In addition, CTRP6 did not affect the viability of normal human gastric epithelial cells. This study suggests that CTRP6 may have potential application in combating stromal fibrosis in diffuse-type gastric cancers.
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- 2021
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42. Downregulation of ARID1A in gastric cancer cells: a putative protective molecular mechanism against the Harakiri-mediated apoptosis pathway
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Itaru Yasufuku, Takuji Sakuratani, Chiemi Saigo, Yusuke Kito, Yoshinori Iwata, Tamotsu Takeuchi, and Kazuhiro Yoshida
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Adult ,Male ,0301 basic medicine ,ARID1A ,Down-Regulation ,Antineoplastic Agents ,Apoptosis ,Sulfides ,Biology ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Mediator ,Downregulation and upregulation ,Stomach Neoplasms ,Cell Line, Tumor ,Biomarkers, Tumor ,Humans ,Molecular Biology ,Gene ,Aged ,Retrospective Studies ,Aged, 80 and over ,Cell Biology ,General Medicine ,Metabolism ,Middle Aged ,Prognosis ,DNA-Binding Proteins ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,030220 oncology & carcinogenesis ,Apoptosis pathway ,Cancer cell ,Cancer research ,Female ,Caprylates ,Apoptosis Regulatory Proteins ,Signal Transduction ,Transcription Factors - Abstract
This study was designed to unravel the pathobiological role of impaired ARID1A expression in gastric carcinogenesis. We examined ARID1A expression immunohistochemically in 98 gastric cancer tissue specimens with regard to the clinicopathological features. Based on the proportion and intensity of ARID1A immunoreactivity at the cancer invasion front, we subdivided the specimens into low- and high-expression ARID1A groups. Notably, low ARID1A expression was significantly correlated with overall survival of the patients. Subsequently, we determined the molecular signature that distinguished ARID1A low/poor prognosis from ARID1A high/good prognosis gastric cancers. A comprehensive gene profiling analysis followed by immunoblotting revealed that a mitochondrial apoptosis mediator, Harakiri, was less expressed in ARID1A low/poor prognosis than ARID1A high/good prognosis gastric cancers. siRNA-mediated ARID1A downregulation significantly reduced expression of the Harakiri molecule in cultured gastric cancer cells. Interestingly, downregulation of ARID1A conferred resistance to apoptosis induced by the mitochondrial metabolism inhibitor, devimistat. In contrast, enforced Harakiri expression restored sensitivity to devimistat-induced apoptosis in ARID1A downregulated gastric cancer cells. The present findings indicate that impaired ARID1A expression might lead to gastric carcinogenesis, putatively through gaining resistance to the Harakiri-mediated apoptosis pathway.
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- 2020
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43. Overexpression of JAG2 is related to poor outcomes in oral squamous cell carcinoma
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Toshiyuki Shibata, Yusuke Kito, Kiichi Hatano, Tamotsu Takeuchi, and Chiemi Saigo
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Male ,Oncology ,JAG2 ,Notch ligand ,medicine.medical_specialty ,Mrna expression ,Kaplan-Meier Estimate ,medicine.disease_cause ,stomatognathic system ,Internal medicine ,Biomarkers, Tumor ,medicine ,Overall survival ,Humans ,Basal cell ,General Dentistry ,Aged ,Squamous Cell Carcinoma of Head and Neck ,business.industry ,Mouth Mucosa ,Cancer ,Original Articles ,Middle Aged ,Prognosis ,medicine.disease ,Immunohistochemistry ,lcsh:RK1-715 ,oral squamous cell carcinoma ,stomatognathic diseases ,Specific antibody ,lcsh:Dentistry ,Original Article ,Female ,Mouth Neoplasms ,Jagged-2 Protein ,business ,Carcinogenesis ,Invasion front - Abstract
Objectives JAG2 is one of Notch ligands, which recently appear to exert various carcinogenesis. In the present study, we aimed to unveil the relation of JAG2 expression and clinicopathological features in oral squamous cell carcinoma (OSCC). Materials and Methods We examined JAG2 expression in OSCC plus adjacent nontumorous epithelia in eight patients. Ninety‐one OSCC tissue specimens were immunohistochemically stained with specific antibodies to JAG2. The immunoreactivities of JAG2 were correlated with clinicopathological factors, including the prognosis of patients. Chi‐square test, Kaplan–Meier survival, and Cox proportional hazard analysis were used to determine the statistical value of JAG2 expression in OSCC. Results JAG2 mRNA expression was much expressed in OSCC tissues compared with adjacent tissue specimens in five of eight patients. JAG2 immunoreactivity was found at invasion front in 31 of 91 OSCC. JAG2 immunoreactivity was significantly associated with age, less than 50 years old of patients (P = .048). Kaplan–Meier analysis demonstrated that the patients with JAG2 immunoreactvty have a short overall survival. With the Cox proportional hazard regression mode, the independent factors predictive of poor overall survival included JAG2 immunoreactivity (P < .05). Conclusions The present findings suggest that JAG2 overexpression, especially at the cancer invasion front, has potential prognostic value.
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- 2020
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44. Facile and secure deployment of plastic stent through an endoscopic tapered sheath for endoscopic ultrasound-guided drainage
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Yusuke Kito, Akihisa Kato, Michihiro Yoshida, Makoto Natsume, Yasuki Hori, Itaru Naitoh, and Kazuki Hayashi
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Treatment Outcome ,Gastroenterology ,Humans ,Drainage ,Stents ,Plastics ,Ultrasonography, Interventional ,Endosonography - Published
- 2022
45. Clinical significance of oxidative stress for untreated patients with diffuse large B‑cell lymphoma
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Takuro Matsumoto, Takeshi Hara, Yusuke Kito, Hiroshi Nakamura, Masahito Shimizu, Hisashi Tsurumi, Ryoko Mabuchi, Tsuyoshi Takami, Junichi Kitagawa, Nobuhiro Kanemura, Tatsuhiko Miyazaki, Tamotsu Takeuchi, Nobuhiko Nakamura, and Soranobu Ninomiya
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Cancer Research ,medicine.medical_specialty ,Antioxidant ,business.industry ,medicine.medical_treatment ,Articles ,medicine.disease ,medicine.disease_cause ,Molecular medicine ,Gastroenterology ,Lymphoma ,Non-Hodgkin's lymphoma ,Oncology ,Apoptosis ,Internal medicine ,medicine ,Clinical significance ,business ,Diffuse large B-cell lymphoma ,Oxidative stress - Abstract
Oxidative stress serves an important role in carcinogenesis. The present study investigated the clinical significance of oxidative stress as a prognostic factor for diffuse large B-cell lymphoma (DLBCL). The participants comprised 55 consecutive patients with DLBCL. A commercially available derivatives of reactive oxygen metabolites (d-ROMs) test kit was used to assess oxidant levels. Similarly, a commercially available biological antioxidant potential (BAP) test was used to assess antioxidant levels. The antioxidative/oxidative stress ratio was calculated as d-ROMs/BAP. The median serum concentration of d-ROMs was 425 µM. The levels of d-ROMs were significantly higher in patients with DLBCL than in healthy volunteers (P
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- 2021
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46. Various innovative roles for 3-Fr microcatheters in pancreaticobiliary endoscopy
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Tadashi Toyohara, Akihisa Kato, Michihiro Yoshida, Kazuki Hayashi, Kenta Kachi, Yasuki Hori, Itaru Naitoh, Go Asano, Hidenori Sahashi, Makoto Natsume, Yusuke Kito, and Hiromi Kataoka
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medicine.medical_specialty ,Catheters ,medicine.diagnostic_test ,business.industry ,Gastroenterology ,Troubleshooting ,Endoscopy, Gastrointestinal ,Endoscopy ,Catheterization ,Catheter ,medicine.anatomical_structure ,medicine ,Cystic duct ,Humans ,Radiology, Nuclear Medicine and imaging ,Radiology ,business - Abstract
With the development of newer devices and technical innovations, pancreaticobiliary endoscopy is expanding to assume more advanced therapeutic roles. As with other devices, slimmed-down "3-Fr microcatheters" are considered to be opening new windows toward entirely new therapeutic techniques for various purposes. Our practical experience with a total of 34 consecutive patients in whom 3-Fr microcatheters were applied during pancreaticobiliary endoscopic procedures clarified the potential roles of this instrument in pancreaticobiliary endoscopy. The major benefits of 3-Fr microcatheters involve their slimness and flexibility. Applications of 3-Fr microcatheters could be categorized into three groups according to the characteristics of usage: (1) utilization as a cannulation catheter for peroral digital cholangioscopy (n = 15); (2) selective advancement through deep flexures or severely stenotic ducts (n = 11); or (3) two-devices-in-one-channel technique (n = 8). The microcatheter worked successfully for cannulation of cholangioscopy in all but one case (14/15, 93.3%). For selective advancement, the microcatheter worked for troubleshooting in 9 of 11 cases (81.8%). With the two-devices-in-one-channel technique, the microcatheter proved satisfactory in all cases (8/8, 100%). In total, the microcatheter was successfully maneuvered in 31 of 34 cases (91.1%), following the failure of procedures using conventional endoscopic techniques. In terms of adverse events, cystic duct injury was only observed in two cases (5.8%), who recovered under conservative observation, because its slimness could minimize the damage. We believe that 3-Fr microcatheters offer effective and safe salvage troubleshooting during various endoscopic pancreaticobiliary procedures that face troublesome situations with conventional strategies.
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- 2021
47. Concomitant Pancreatic Ductal Adenocarcinoma and Type 1 Autoimmune Pancreatitis: A Potential Issue in the Diagnosis of Carcinoma by Endoscopic Ultrasound-guided Fine-needle Biopsy.
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Kenta Kachi, Itaru Naitoh, Tesshin Ban, Kazuki Hayashi, Michihiro Yoshida, Yasuki Hori, Makoto Natsume, Akihisa Kato, Yusuke Kito, Kenta Saito, Yoichi Matsuo, Hiroyuki Kato, Aya Naiki-Ito, Satoru Takahashi, Kenji Notohara, and Hiromi Kataoka
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- 2023
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48. Incidental cytokeratin-positive interstitial reticulum cell tumor of the lymph node accompanied by breast cancer: Status of YAP/TAZ expression in tumor cells
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Shusuke Nomura, Masayoshi Hasegawa, Manabu Futamura, Takuya Mikamo, Ryutaro Mori, Yusuke Kito, Yuki Hanamatsu, Tamotsu Takeuchi, Chiemi Saigo, and Kazuhiro Yoshida
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CD31 ,Pathology ,medicine.medical_specialty ,Axillary lymph nodes ,CD68 ,business.industry ,medicine.medical_treatment ,medicine.disease ,Pathology and Forensic Medicine ,Lymphoid stromal tumor ,medicine.anatomical_structure ,Breast cancer ,Reticular cell ,Fibroblastic reticular cells ,medicine ,YAP/TAZ ,RB1-214 ,Lymph ,business ,Lymph node ,Mastectomy ,Cytokeratin-positive interstitial reticulum cell tumor - Abstract
Approximately 24 cases of cytokeratin-positive interstitial reticulum cell tumors have been reported in English literature. In this paper, we report a case of cytokeratin-positive interstitial reticulum cell tumor that was localized in the parafollicular region of the axillary lymph node and was an incidental finding in a patient with breast cancer. A 50 year-old woman underwent a mastectomy and lymph node dissection following a preoperative diagnosis of bilateral breast cancer. Histopathological examination revealed that the patient had non-invasive and invasive ductal carcinoma in the right and left mammary glands. Although we could not detect breast cancer metastasis in the lymph nodes, one of eight right axillary lymph nodes harbored an 11 × 8 mm discohesive sheet of large polygonal epithelioid tumor cells in the parafollicular area. Tumor cells were immunoreactive for AE1/AE3, CAM5.2, CK18 and l-caldesmon and negative for CD1a, CD21, CD23, CD31, CD68, and S‑100 protein markers. Based on these findings, the lesion was diagnosed as a cytokeratin-positive interstitial reticulum cell tumor of the lymph node. Notably, this early-stage cytokeratin-positive interstitial reticulum cell tumor demonstrated focal nuclear YAP/TAZ proto-oncogene products, which were recently identified as critical for differentiation, maturation, and function of fibroblastic reticular cells. To the best of our knowledge, this is the first report to describe nuclear YAP/TAZ immunoreactivity in cytokeratin-positive interstitial reticulum cell tumors.
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- 2021
49. Impact of initial empirical antimicrobial choice and cause of in-hospital death in patients with nursing and healthcare-associated pneumonia (NHCAP): A retrospective study
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Kenichi, Kato, Kazunobu, Kuwabara, Kiyotaka, Ono, Yusuke, Kito, Tatsuyoshi, Yokoi, Takazumi, Yoshida, Keisuke, Kato, Masahiro, Hirose, Daijo, Inaguma, and Takahiko, Horiguchi
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Empiric antimicrobial therapy ,Pneumonia ,Nursing and healthcare-associated pneumonia - Abstract
To examine the differences in antimicrobial selection outcomes in nursing and healthcare-associated pneumonia (NHCAP) patients with and without risk factors for drug-resistant pathogen (RDRP) infection, and to identify the cause of in-hospital death after improvement of NHCAP.We conducted a retrospective analysis of the medical records of hospitalized adult patients with NHCAP. NHCAP patients were divided into the RDRP and non-RDRP groups. The RDRP group was further classified into the narrow and broad subgroups according to the type of empirical antimicrobial agent selected. The difference in mortality between these subgroups was then examined. The cause of all in-hospital deaths was also evaluated.e evaluated 220 patients with NHCAP. There was no difference in mortality between the narrow and broad subgroups (11.8% vs. 15.4%, p=0.655). Among the group with improved NHCAP, 11.3% (n=23/203) died in hospital before discharge. Although the causes of death in patients who improved after NHCAP were diverse, the most common was recurrence of pneumonia.Empirical antimicrobial therapy for NHCAP may not always require selection of broad-spectrum antimicrobial agents, as has been previously reported. Patients with NHCAP may die from other causes, even after NHCAP has improved.
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- 2021
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50. An adiponectin paralog protein, CTRP6 decreased the proliferation and invasion activity of oral squamous cell carcinoma cells: possible interaction with laminin receptor pathway
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Chiemi Saigo, Yusuke Kito, Kiichi Hatano, Kimika Hano, Toshiyuki Shibata, and Tamotsu Takeuchi
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Male ,Mice, SCID ,Inhibitory postsynaptic potential ,medicine.disease_cause ,law.invention ,Receptors, Laminin ,Mice ,Adipokines ,law ,Laminin ,Cell Line, Tumor ,Genetics ,medicine ,Animals ,Humans ,Neoplasm Invasiveness ,Receptor ,Molecular Biology ,Cell Proliferation ,Adiponectin ,biology ,Chemistry ,Binding protein ,General Medicine ,Xenograft Model Antitumor Assays ,Carcinoma, Squamous Cell ,Recombinant DNA ,Cancer research ,biology.protein ,Mouth Neoplasms ,Tumor necrosis factor alpha ,Collagen ,Carcinogenesis - Abstract
C1q/tumor necrosis factor-related protein-6 (CTRP6), also known as CTRP6 is identified adiponectin paralog. Although recent studies have revealed that adiponectin has an inhibitory role in carcinogenesis, the role of CTRP6 in carcinogenesis remains unclear. In this study, we found that eukaryotic recombinant CTRP6 protein bound to the cell surface membrane of cultured oral squamous cell carcinoma cells by immunofluorescence staining. Screening of CTRP6 binding protein in expression library followed by co-immunoprecipitation assay revealed that CTRP6 bound to the precursor of laminin receptor. CTRP6 disturbed the binding of laminin to the laminin receptor. Interestingly, the eukaryotic recombinant CTRP6 protein significantly suppressed the proliferation and Matrigel invasion activity of oral squamous cell carcinoma SAS cells in a dose-dependent manner. Moreover, administration of CTRP6 significantly attenuated the growth of SAS cells in xenoplant mice model. Laminin and laminin receptor are known to be overexpressed and promote the tumor growth in OSCC. Combined together, the present findings suggest that CTRP6 could repress progression of oral squamous cell carcinoma cells, putatively through disrupting the laminin-laminin receptor axis.
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- 2019
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